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UniProtKB - P0AEC3 (ARCB_ECOLI)

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Protein

Aerobic respiration control sensor protein ArcB

Gene

arcB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionKinase, Transferase
Biological processTranscription, Transcription regulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARCB-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Aerobic respiration control sensor protein ArcB (EC:2.7.13.3)
Gene namesi
Name:arcB
Ordered Locus Names:b3210, JW5536
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10062 arcB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Topological domaini47 – 57PeriplasmicSequence analysisAdd BLAST11
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Topological domaini79 – 778CytoplasmicSequence analysisAdd BLAST700

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
View the complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi292H → Q: Loss of activity. 1 Publication1
Mutagenesisi576D → A: Loss of activity. 1 Publication1
Mutagenesisi717H → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746841 – 778Aerobic respiration control sensor protein ArcBAdd BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei292Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1
Modified residuei5764-aspartylphosphateCurated1
Modified residuei717Phosphohistidine1 Publication1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AEC3
PaxDbiP0AEC3
PRIDEiP0AEC3

PTM databases

iPTMnetiP0AEC3

Interactioni

Protein-protein interaction databases

BioGridi4263422, 413 interactors
DIPiDIP-47915N
IntActiP0AEC3, 7 interactors
MINTiP0AEC3
STRINGi316385.ECDH10B_3385

Structurei

Secondary structure

1778
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi27 – 45Combined sources19
Beta strandi50 – 54Combined sources5
Turni58 – 60Combined sources3
Helixi61 – 78Combined sources18
Helixi660 – 664Combined sources5
Helixi667 – 676Combined sources10
Helixi679 – 705Combined sources27
Helixi709 – 725Combined sources17
Helixi729 – 738Combined sources10
Helixi746 – 775Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0BX-ray2.06A654-778[»]
1BDJX-ray2.68B654-778[»]
1FR0NMR-A654-778[»]
2A0BX-ray1.57A654-778[»]
2KSDNMR-A1-115[»]
ProteinModelPortaliP0AEC3
SMRiP0AEC3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEC3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 223PASPROSITE-ProRule annotationAdd BLAST71
Domaini226 – 278PACPROSITE-ProRule annotationAdd BLAST53
Domaini289 – 507Histidine kinasePROSITE-ProRule annotationAdd BLAST219
Domaini527 – 643Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini678 – 771HPtPROSITE-ProRule annotationAdd BLAST94

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
COG0642 LUCA
COG0784 LUCA
COG2198 LUCA
HOGENOMiHOG000272667
InParanoidiP0AEC3
KOiK07648
OMAiAMLEQYI
PhylomeDBiP0AEC3

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
cd00088 HPT, 1 hit
cd00130 PAS, 1 hit
cd00156 REC, 1 hit
Gene3Di1.10.287.970, 1 hit
1.20.120.160, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR027460 ArcB_TM_sf
IPR011006 CheY-like_superfamily
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR036641 HPT_dom_sf
IPR000014 PAS
IPR000700 PAS-assoc_C
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR004358 Sig_transdc_His_kin-like_C
IPR008207 Sig_transdc_His_kin_Hpt_dom
IPR014409 Sig_transdc_His_kin_hyb_ArcB
IPR001789 Sig_transdc_resp-reg_receiver
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PF01627 Hpt, 1 hit
PF00989 PAS, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF003182 ArcB, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SM00073 HPT, 1 hit
SM00091 PAS, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF47226 SSF47226, 1 hit
SSF47384 SSF47384, 1 hit
SSF52172 SSF52172, 1 hit
SSF55785 SSF55785, 1 hit
SSF55874 SSF55874, 1 hit
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit
PS50894 HPT, 1 hit
PS50113 PAC, 1 hit
PS50112 PAS, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV 
        60         70         80         90        100
ESIDVIRSIF FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER 
       110        120        130        140        150
DLSLNVQLKD NIAQLNQEIA VREKAEAELQ ETFGQLKIEI KEREETQIQL 
       160        170        180        190        200
EQQSSFLRSF LDASPDLVFY RNEDKEFSGC NRAMELLTGK SEKQLVHLKP 
       210        220        230        240        250
ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA CFEIRKVPYY 
       260        270        280        290        300
DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG 
       310        320        330        340        350
IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ 
       360        370        380        390        400
LDNQPVDFTS FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR 
       410        420        430        440        450
QILWNLISNA VKFTQQGQVT VRVRYDEGDM LHFEVEDSGI GIPQDELDKI 
       460        470        480        490        500
FAMYYQVKDS HGGKPATGTG IGLAVSRRLA KNMGGDITVT SEQGKGSTFT 
       510        520        530        540        550
LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV ARSVLEKLGN 
       560        570        580        590        600
SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL 
       610        620        630        640        650
PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE 
       660        670        680        690        700
STVTTEENSK SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL 
       710        720        730        740        750
ESNLTAQDKK GIVEEGHKIK GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV 
       760        770 
GEWIEEMKEE WRHDVEVLKA WVAKATKK
Length:778
Mass (Da):87,983
Last modified:December 6, 2005 - v1
Checksum:iDD61EA6ECF95AD30
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti469 – 470Missing in AAA58012 (PubMed:9278503).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53315 Genomic DNA Translation: CAA37397.1
U18997 Genomic DNA Translation: AAA58012.1
U00096 Genomic DNA Translation: AAT48172.1
AP009048 Genomic DNA Translation: BAE77254.1
PIRiD65112 RGECAR
RefSeqiWP_000809774.1, NZ_LN832404.1
YP_026207.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48172; AAT48172; b3210
BAE77254; BAE77254; BAE77254
GeneIDi947887
KEGGiecj:JW5536
eco:b3210
PATRICifig|1411691.4.peg.3519

Similar proteinsi

Entry informationi

Entry nameiARCB_ECOLI
AccessioniPrimary (citable) accession number: P0AEC3
Secondary accession number(s): P22763, Q2M902
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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