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P0AEC3

- ARCB_ECOLI

UniProt

P0AEC3 - ARCB_ECOLI

Protein

Aerobic respiration control sensor protein ArcB

Gene

arcB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.

    Catalytic activityi

    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. histidine phosphotransfer kinase activity Source: EcoCyc
    3. phosphorelay response regulator activity Source: InterPro
    4. phosphorelay sensor kinase activity Source: EcoCyc

    GO - Biological processi

    1. phosphorelay signal transduction system Source: EcoCyc
    2. protein autophosphorylation Source: CACAO
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. signal transduction by phosphorylation Source: GOC
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ARCB-MONOMER.
    ECOL316407:JW5536-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aerobic respiration control sensor protein ArcB (EC:2.7.13.3)
    Gene namesi
    Name:arcB
    Ordered Locus Names:b3210, JW5536
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10062. arcB.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: EcoCyc
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi292 – 2921H → Q: Loss of activity. 1 Publication
    Mutagenesisi576 – 5761D → A: Loss of activity. 1 Publication
    Mutagenesisi717 – 7171H → Q: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 778778Aerobic respiration control sensor protein ArcBPRO_0000074684Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei292 – 2921Phosphohistidine; by autocatalysis1 PublicationPROSITE-ProRule annotation
    Modified residuei576 – 57614-aspartylphosphateCurated
    Modified residuei717 – 7171Phosphohistidine1 Publication

    Post-translational modificationi

    Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP0AEC3.
    PRIDEiP0AEC3.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AEC3.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47915N.
    IntActiP0AEC3. 7 interactions.
    MINTiMINT-98513.
    STRINGi511145.b3210.

    Structurei

    Secondary structure

    1
    778
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi27 – 4519
    Beta strandi50 – 545
    Turni58 – 603
    Helixi61 – 7818
    Helixi660 – 6645
    Helixi667 – 67610
    Helixi679 – 70527
    Helixi709 – 72517
    Helixi729 – 73810
    Helixi746 – 77530

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A0BX-ray2.06A654-778[»]
    1BDJX-ray2.68B654-778[»]
    1FR0NMR-A654-778[»]
    2A0BX-ray1.57A654-778[»]
    2KSDNMR-A1-115[»]
    ProteinModelPortaliP0AEC3.
    SMRiP0AEC3. Positions 15-89, 276-646, 659-776.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEC3.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini47 – 5711PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini79 – 778700CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei58 – 7821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 22371PASPROSITE-ProRule annotationAdd
    BLAST
    Domaini226 – 27853PACPROSITE-ProRule annotationAdd
    BLAST
    Domaini289 – 507219Histidine kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini527 – 643117Response regulatoryPROSITE-ProRule annotationAdd
    BLAST
    Domaini678 – 77194HPtPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 histidine kinase domain.PROSITE-ProRule annotation
    Contains 1 HPt domain.PROSITE-ProRule annotation
    Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
    Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0642.
    HOGENOMiHOG000272667.
    KOiK07648.
    OMAiAPRLWQR.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP0AEC3.

    Family and domain databases

    Gene3Di1.10.287.130. 1 hit.
    1.10.287.970. 1 hit.
    1.20.120.160. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR027460. ArcB_TM.
    IPR011006. CheY-like_superfamily.
    IPR003661. EnvZ-like_dim/P.
    IPR003594. HATPase_ATP-bd.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR013767. PAS_fold.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR008207. Sig_transdc_His_kin_Hpt_dom.
    IPR014409. Sig_transdc_His_kin_hyb_ArcB.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    PF01627. Hpt. 1 hit.
    PF00989. PAS. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003182. ArcB. 1 hit.
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    SM00073. HPT. 1 hit.
    SM00091. PAS. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47226. SSF47226. 1 hit.
    SSF47384. SSF47384. 1 hit.
    SSF52172. SSF52172. 1 hit.
    SSF55785. SSF55785. 1 hit.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50109. HIS_KIN. 1 hit.
    PS50894. HPT. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AEC3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV    50
    ESIDVIRSIF FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER 100
    DLSLNVQLKD NIAQLNQEIA VREKAEAELQ ETFGQLKIEI KEREETQIQL 150
    EQQSSFLRSF LDASPDLVFY RNEDKEFSGC NRAMELLTGK SEKQLVHLKP 200
    ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA CFEIRKVPYY 250
    DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG 300
    IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ 350
    LDNQPVDFTS FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR 400
    QILWNLISNA VKFTQQGQVT VRVRYDEGDM LHFEVEDSGI GIPQDELDKI 450
    FAMYYQVKDS HGGKPATGTG IGLAVSRRLA KNMGGDITVT SEQGKGSTFT 500
    LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV ARSVLEKLGN 550
    SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL 600
    PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE 650
    STVTTEENSK SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL 700
    ESNLTAQDKK GIVEEGHKIK GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV 750
    GEWIEEMKEE WRHDVEVLKA WVAKATKK 778
    Length:778
    Mass (Da):87,983
    Last modified:December 6, 2005 - v1
    Checksum:iDD61EA6ECF95AD30
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti469 – 4702Missing in AAA58012. (PubMed:9278503)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53315 Genomic DNA. Translation: CAA37397.1.
    U18997 Genomic DNA. Translation: AAA58012.1.
    U00096 Genomic DNA. Translation: AAT48172.1.
    AP009048 Genomic DNA. Translation: BAE77254.1.
    PIRiD65112. RGECAR.
    RefSeqiYP_026207.1. NC_000913.3.
    YP_491395.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAT48172; AAT48172; b3210.
    BAE77254; BAE77254; BAE77254.
    GeneIDi12934428.
    947887.
    KEGGiecj:Y75_p3130.
    eco:b3210.
    PATRICi32121840. VBIEscCol129921_3304.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53315 Genomic DNA. Translation: CAA37397.1 .
    U18997 Genomic DNA. Translation: AAA58012.1 .
    U00096 Genomic DNA. Translation: AAT48172.1 .
    AP009048 Genomic DNA. Translation: BAE77254.1 .
    PIRi D65112. RGECAR.
    RefSeqi YP_026207.1. NC_000913.3.
    YP_491395.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A0B X-ray 2.06 A 654-778 [» ]
    1BDJ X-ray 2.68 B 654-778 [» ]
    1FR0 NMR - A 654-778 [» ]
    2A0B X-ray 1.57 A 654-778 [» ]
    2KSD NMR - A 1-115 [» ]
    ProteinModelPortali P0AEC3.
    SMRi P0AEC3. Positions 15-89, 276-646, 659-776.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47915N.
    IntActi P0AEC3. 7 interactions.
    MINTi MINT-98513.
    STRINGi 511145.b3210.

    Proteomic databases

    PaxDbi P0AEC3.
    PRIDEi P0AEC3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT48172 ; AAT48172 ; b3210 .
    BAE77254 ; BAE77254 ; BAE77254 .
    GeneIDi 12934428.
    947887.
    KEGGi ecj:Y75_p3130.
    eco:b3210.
    PATRICi 32121840. VBIEscCol129921_3304.

    Organism-specific databases

    EchoBASEi EB0060.
    EcoGenei EG10062. arcB.

    Phylogenomic databases

    eggNOGi COG0642.
    HOGENOMi HOG000272667.
    KOi K07648.
    OMAi APRLWQR.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P0AEC3.

    Enzyme and pathway databases

    BioCyci EcoCyc:ARCB-MONOMER.
    ECOL316407:JW5536-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AEC3.
    PROi P0AEC3.

    Gene expression databases

    Genevestigatori P0AEC3.

    Family and domain databases

    Gene3Di 1.10.287.130. 1 hit.
    1.10.287.970. 1 hit.
    1.20.120.160. 1 hit.
    3.30.565.10. 1 hit.
    InterProi IPR027460. ArcB_TM.
    IPR011006. CheY-like_superfamily.
    IPR003661. EnvZ-like_dim/P.
    IPR003594. HATPase_ATP-bd.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR013767. PAS_fold.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR008207. Sig_transdc_His_kin_Hpt_dom.
    IPR014409. Sig_transdc_His_kin_hyb_ArcB.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    PF01627. Hpt. 1 hit.
    PF00989. PAS. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003182. ArcB. 1 hit.
    PRINTSi PR00344. BCTRLSENSOR.
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    SM00073. HPT. 1 hit.
    SM00091. PAS. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47226. SSF47226. 1 hit.
    SSF47384. SSF47384. 1 hit.
    SSF52172. SSF52172. 1 hit.
    SSF55785. SSF55785. 1 hit.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50109. HIS_KIN. 1 hit.
    PS50894. HPT. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The arcB gene of Escherichia coli encodes a sensor-regulator protein for anaerobic repression of the arc modulon."
      Iuchi S., Matsuda Z., Fujiwara T., Lin E.C.C.
      Mol. Microbiol. 4:715-727(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: SEQUENCE REVISION TO 469-470.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "In vitro phosphorylation study of the arc two-component signal transduction system of Escherichia coli."
      Georgellis D., Lynch A.S., Lin E.C.C.
      J. Bacteriol. 179:5429-5435(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: M15.
    6. "Signal decay through a reverse phosphorelay in the arc two-component signal transduction system."
      Georgellis D., Kwon O., De Wulf P., Lin E.C.C.
      J. Biol. Chem. 273:32864-32869(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: M15.
    7. "Phosphorelay as the sole physiological route of signal transmission by the arc two-component system of Escherichia coli."
      Kwon O., Georgellis D., Lin E.C.C.
      J. Bacteriol. 182:3858-3862(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT HIS-292 AND HIS-717, MUTAGENESIS OF HIS-292; ASP-576 AND HIS-717.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    8. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: K12 / MG1655 / ATCC 47076.
    9. "Insights into multistep phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB."
      Kato M., Mizuno T., Shimizu T., Hakoshima T.
      Cell 88:717-723(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 660-778.
    10. "Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY."
      Kato M., Mizuno T., Hakoshima T.
      Acta Crystallogr. D 54:140-142(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 659-776 IN COMPLEX WITH CHEY.
    11. "Refined structure of the histidine-containing-phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57-A resolution."
      Kato M., Mizuno T., Shimizu T., Hakoshima T.
      Acta Crystallogr. D 55:1842-1849(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 659-776.

    Entry informationi

    Entry nameiARCB_ECOLI
    AccessioniPrimary (citable) accession number: P0AEC3
    Secondary accession number(s): P22763, Q2M902
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3