UniProtKB - P0AEC3 (ARCB_ECOLI)
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Protein
Aerobic respiration control sensor protein ArcB
Gene
arcB
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.
Catalytic activityi
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histidine phosphotransfer kinase activity Source: EcoCyc
- phosphorelay sensor kinase activity Source: EcoCyc
GO - Biological processi
- phosphorelay signal transduction system Source: EcoCyc
- protein autophosphorylation Source: EcoCyc
- regulation of transcription, DNA-templated Source: UniProtKB-KW
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Transcription, Transcription regulation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ARCB-MONOMER. |
BRENDAi | 2.7.13.3. 2026. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:arcB Ordered Locus Names:b3210, JW5536 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG10062. arcB. |
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 25 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 26 – 46 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 47 – 57 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 58 – 78 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 79 – 778 | CytoplasmicSequence analysisAdd BLAST | 700 |
GO - Cellular componenti
- integral component of membrane Source: EcoCyc
- intracellular Source: GOC
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 292 | H → Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 576 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 717 | H → Q: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074684 | 1 – 778 | Aerobic respiration control sensor protein ArcBAdd BLAST | 778 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 292 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 576 | 4-aspartylphosphateCurated | 1 | |
Modified residuei | 717 | Phosphohistidine1 Publication | 1 |
Post-translational modificationi
Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P0AEC3. |
PaxDbi | P0AEC3. |
PRIDEi | P0AEC3. |
PTM databases
iPTMneti | P0AEC3. |
Interactioni
Protein-protein interaction databases
BioGridi | 4263422. 413 interactors. |
DIPi | DIP-47915N. |
IntActi | P0AEC3. 8 interactors. |
MINTi | P0AEC3. |
STRINGi | 316385.ECDH10B_3385. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 27 – 45 | Combined sources | 19 | |
Beta strandi | 50 – 54 | Combined sources | 5 | |
Turni | 58 – 60 | Combined sources | 3 | |
Helixi | 61 – 78 | Combined sources | 18 | |
Helixi | 660 – 664 | Combined sources | 5 | |
Helixi | 667 – 676 | Combined sources | 10 | |
Helixi | 679 – 705 | Combined sources | 27 | |
Helixi | 709 – 725 | Combined sources | 17 | |
Helixi | 729 – 738 | Combined sources | 10 | |
Helixi | 746 – 775 | Combined sources | 30 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A0B | X-ray | 2.06 | A | 654-778 | [»] | |
1BDJ | X-ray | 2.68 | B | 654-778 | [»] | |
1FR0 | NMR | - | A | 654-778 | [»] | |
2A0B | X-ray | 1.57 | A | 654-778 | [»] | |
2KSD | NMR | - | A | 1-115 | [»] | |
ProteinModelPortali | P0AEC3. | |||||
SMRi | P0AEC3. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEC3. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 153 – 223 | PASPROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 226 – 278 | PACPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 289 – 507 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 219 | |
Domaini | 527 – 643 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 678 – 771 | HPtPROSITE-ProRule annotationAdd BLAST | 94 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4105BZU. Bacteria. COG0642. LUCA. COG0784. LUCA. COG2198. LUCA. |
HOGENOMi | HOG000272667. |
InParanoidi | P0AEC3. |
KOi | K07648. |
OMAi | AMLEQYI. |
PhylomeDBi | P0AEC3. |
Family and domain databases
CDDi | cd00075. HATPase_c. 1 hit. cd00082. HisKA. 1 hit. cd00088. HPT. 1 hit. cd00130. PAS. 1 hit. cd00156. REC. 1 hit. |
Gene3Di | 1.10.287.970. 1 hit. 1.20.120.160. 1 hit. 3.30.565.10. 1 hit. |
InterProi | View protein in InterPro IPR027460. ArcB_TM_sf. IPR011006. CheY-like_superfamily. IPR003594. HATPase_C. IPR036890. HATPase_C_sf. IPR005467. His_kinase_dom. IPR003661. HisK_dim/P. IPR036097. HisK_dim/P_sf. IPR036641. HPT_dom_sf. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR035965. PAS-like_dom_sf. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_Hpt_dom. IPR014409. Sig_transdc_His_kin_hyb_ArcB. IPR001789. Sig_transdc_resp-reg_receiver. |
Pfami | View protein in Pfam PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF01627. Hpt. 1 hit. PF00989. PAS. 1 hit. PF00072. Response_reg. 1 hit. |
PIRSFi | PIRSF003182. ArcB. 1 hit. |
PRINTSi | PR00344. BCTRLSENSOR. |
SMARTi | View protein in SMART SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00073. HPT. 1 hit. SM00091. PAS. 1 hit. SM00448. REC. 1 hit. |
SUPFAMi | SSF47226. SSF47226. 1 hit. SSF47384. SSF47384. 1 hit. SSF52172. SSF52172. 1 hit. SSF55785. SSF55785. 1 hit. SSF55874. SSF55874. 1 hit. |
TIGRFAMsi | TIGR00229. sensory_box. 1 hit. |
PROSITEi | View protein in PROSITE PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. |
i Sequence
Sequence statusi: Complete.
P0AEC3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV
60 70 80 90 100
ESIDVIRSIF FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER
110 120 130 140 150
DLSLNVQLKD NIAQLNQEIA VREKAEAELQ ETFGQLKIEI KEREETQIQL
160 170 180 190 200
EQQSSFLRSF LDASPDLVFY RNEDKEFSGC NRAMELLTGK SEKQLVHLKP
210 220 230 240 250
ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA CFEIRKVPYY
260 270 280 290 300
DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG
310 320 330 340 350
IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ
360 370 380 390 400
LDNQPVDFTS FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR
410 420 430 440 450
QILWNLISNA VKFTQQGQVT VRVRYDEGDM LHFEVEDSGI GIPQDELDKI
460 470 480 490 500
FAMYYQVKDS HGGKPATGTG IGLAVSRRLA KNMGGDITVT SEQGKGSTFT
510 520 530 540 550
LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV ARSVLEKLGN
560 570 580 590 600
SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL
610 620 630 640 650
PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE
660 670 680 690 700
STVTTEENSK SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL
710 720 730 740 750
ESNLTAQDKK GIVEEGHKIK GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV
760 770
GEWIEEMKEE WRHDVEVLKA WVAKATKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 469 – 470 | Missing in AAA58012 (PubMed:9278503).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53315 Genomic DNA. Translation: CAA37397.1. U18997 Genomic DNA. Translation: AAA58012.1. U00096 Genomic DNA. Translation: AAT48172.1. AP009048 Genomic DNA. Translation: BAE77254.1. |
PIRi | D65112. RGECAR. |
RefSeqi | WP_000809774.1. NZ_LN832404.1. YP_026207.1. NC_000913.3. |
Genome annotation databases
EnsemblBacteriai | AAT48172; AAT48172; b3210. BAE77254; BAE77254; BAE77254. |
GeneIDi | 947887. |
KEGGi | ecj:JW5536. eco:b3210. |
PATRICi | fig|1411691.4.peg.3519. |
Similar proteinsi
Entry informationi
Entry namei | ARCB_ECOLI | |
Accessioni | P0AEC3Primary (citable) accession number: P0AEC3 Secondary accession number(s): P22763, Q2M902 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | March 28, 2018 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |