Reviewed, UniProtKB/Swiss-Prot P0AEC3 (ARCB_ECOLI)

Names and origin

Protein names

Recommended name:
    Aerobic respiration control sensor protein arcB
    EC=2.7.13.3

Gene names

Name:	arcB
Ordered Locus Names:	b3210, JW5536

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system arcB/arcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates arcA via a four-step phosphorelay. ArcB can also dephosphorylate arcA by a reverse phosphorelay involving His-717 and Asp-576.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.8.
Post-translational modification	Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 HPt domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 778

778

Aerobic respiration control sensor protein arcB

PRO_0000074684

Regions

Topological domain

1 – 25

25

Cytoplasmic Potential

Transmembrane

26 – 46

21

Potential

Topological domain

47 – 57

11

Periplasmic Potential

Transmembrane

58 – 78

21

Potential

Topological domain

79 – 778

700

Cytoplasmic Potential

Domain

153 – 223

71

PAS

Domain

226 – 278

53

PAC

Domain

289 – 507

219

Histidine kinase

Domain

527 – 643

117

Response regulatory

Domain

678 – 771

94

HPt

Amino acid modifications

Modified residue

292

1

Phosphohistidine; by autocatalysis

Modified residue

576

1

4-aspartylphosphate Probable

Modified residue

717

1

Phosphohistidine Probable

Experimental info

Mutagenesis

292

1

H → Q: Loss of activity. Ref.7

Mutagenesis

576

1

D → A: Loss of activity. Ref.7

Mutagenesis

717

1

H → Q: Loss of activity. Ref.7

Sequence conflict

469 – 470

2

Missing in AAA58012. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

778

Helix Strand Turn

Details...

Helix

661 – 664

4

Helix

667 – 676

10

Helix

679 – 705

27

Helix

709 – 725

17

Helix

729 – 738

10

Helix

746 – 775

30

Sequences

	Sequence		Length	Mass (Da)
	P0AEC3-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: DD61EA6ECF95AD30	FASTA	778	87,983
	10 20 30 40 50 60 MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV ESIDVIRSIF 70 80 90 100 110 120 FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER DLSLNVQLKD NIAQLNQEIA 130 140 150 160 170 180 VREKAEAELQ ETFGQLKIEI KEREETQIQL EQQSSFLRSF LDASPDLVFY RNEDKEFSGC 190 200 210 220 230 240 NRAMELLTGK SEKQLVHLKP ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA 250 260 270 280 290 300 CFEIRKVPYY DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG 310 320 330 340 350 360 IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ LDNQPVDFTS 370 380 390 400 410 420 FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR QILWNLISNA VKFTQQGQVT 430 440 450 460 470 480 VRVRYDEGDM LHFEVEDSGI GIPQDELDKI FAMYYQVKDS HGGKPATGTG IGLAVSRRLA 490 500 510 520 530 540 KNMGGDITVT SEQGKGSTFT LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV 550 560 570 580 590 600 ARSVLEKLGN SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL 610 620 630 640 650 660 PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE STVTTEENSK 670 680 690 700 710 720 SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL ESNLTAQDKK GIVEEGHKIK 730 740 750 760 770 GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV GEWIEEMKEE WRHDVEVLKA WVAKATKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The arcB gene of Escherichia coli encodes a sensor-regulator protein for anaerobic repression of the arc modulon." Iuchi S., Matsuda Z., Fujiwara T., Lin E.C.C. Mol. Microbiol. 4:715-727(1990) [PubMed: 2201868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION TO 469-470.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"In vitro phosphorylation study of the arc two-component signal transduction system of Escherichia coli." Georgellis D., Lynch A.S., Lin E.C.C. J. Bacteriol. 179:5429-5435(1997) [PubMed: 9286997] [Abstract] Cited for: CHARACTERIZATION. Strain: M15.
[6]	"Signal decay through a reverse phosphorelay in the arc two-component signal transduction system." Georgellis D., Kwon O., De Wulf P., Lin E.C.C. J. Biol. Chem. 273:32864-32869(1998) [PubMed: 9830034] [Abstract] Cited for: CHARACTERIZATION. Strain: M15.
[7]	"Phosphorelay as the sole physiological route of signal transmission by the arc two-component system of Escherichia coli." Kwon O., Georgellis D., Lin E.C.C. J. Bacteriol. 182:3858-3862(2000) [PubMed: 10851007] [Abstract] Cited for: MUTAGENESIS OF HIS-292; ASP-576 AND HIS-717. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
[9]	"Insights into multistep phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB." Kato M., Mizuno T., Shimizu T., Hakoshima T. Cell 88:717-723(1997) [PubMed: 9054511] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 660-778.
[10]	"Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY." Kato M., Mizuno T., Hakoshima T. Acta Crystallogr. D 54:140-142(1998) [PubMed: 9761838] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 659-776 IN COMPLEX WITH CHEY.
[11]	"Refined structure of the histidine-containing-phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli at 1.57-A resolution." Kato M., Mizuno T., Shimizu T., Hakoshima T. Acta Crystallogr. D 55:1842-1849(1999) [PubMed: 10531481] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 659-776.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X53315 Genomic DNA. Translation: CAA37397.1.
U18997 Genomic DNA. Translation: AAA58012.1.
U00096 Genomic DNA. Translation: AAT48172.1.
AP009048 Genomic DNA. Translation: BAE77254.1.

PIR

RGECAR. D65112.

RefSeq

AP_003753.1.
YP_026207.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A0B	X-ray	2.06	A	654-778	[»]
1BDJ	X-ray	2.68	B	654-778	[»]
1FR0	NMR	-	A	654-778	[»]
2A0B	X-ray	1.57	A	654-778	[»]

SMR

P0AEC3. Positions 150-301, 264-504, 525-720.

ModBase

Search...

Protein-protein interaction databases

STRING

P0AEC3.

Genome annotation databases

GeneID

947887.

GenomeReviews

Gene locus JW5536 in contig AP009048_GR.
Gene locus b3210 in contig U00096_GR.

KEGG

ecj:JW5536.
eco:b3210.

Organism-specific databases

EchoBASE

EB0060.

EcoGene

EG10062. arcB.

CMR

Search...

Phylogenomic databases

eggNOG

COG0642.

HOGENOM

HBG297001.

OMA

AGADAME.

Enzyme and pathway databases

BioCyc

EcoCyc:ARCB-MONOMER.

Gene expression databases

Genevestigator

P0AEC3.

Family and domain databases

InterPro

IPR003594. ATPase-like_ATP-bd.
IPR011006. CheY-like.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014409. Sig_transdc_His_kin_hyb_ArcB.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]

Gene3D

G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.

Pfam

PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]

PIRSF

PIRSF003182. ArcB. 1 hit.

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00229. sensory_box. 1 hit.

PROSITE

PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ARCB_ECOLI

Accession

Primary (citable) accession number: P0AEC3
Secondary accession number(s): P22763, Q2M902

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)