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Protein

Aerobic respiration control sensor protein ArcB

Gene

arcB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: CACAO
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARCB-MONOMER.
ECOL316407:JW5536-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Aerobic respiration control sensor protein ArcB (EC:2.7.13.3)
Gene namesi
Name:arcB
Ordered Locus Names:b3210, JW5536
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10062. arcB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Topological domaini47 – 57PeriplasmicSequence analysisAdd BLAST11
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Topological domaini79 – 778CytoplasmicSequence analysisAdd BLAST700

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi292H → Q: Loss of activity. 1 Publication1
Mutagenesisi576D → A: Loss of activity. 1 Publication1
Mutagenesisi717H → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746841 – 778Aerobic respiration control sensor protein ArcBAdd BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei292Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1
Modified residuei5764-aspartylphosphateCurated1
Modified residuei717Phosphohistidine1 Publication1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AEC3.
PaxDbiP0AEC3.
PRIDEiP0AEC3.

PTM databases

iPTMnetiP0AEC3.

Interactioni

Protein-protein interaction databases

BioGridi4263422. 370 interactors.
DIPiDIP-47915N.
IntActiP0AEC3. 7 interactors.
MINTiMINT-98513.
STRINGi511145.b3210.

Structurei

Secondary structure

1778
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi27 – 45Combined sources19
Beta strandi50 – 54Combined sources5
Turni58 – 60Combined sources3
Helixi61 – 78Combined sources18
Helixi660 – 664Combined sources5
Helixi667 – 676Combined sources10
Helixi679 – 705Combined sources27
Helixi709 – 725Combined sources17
Helixi729 – 738Combined sources10
Helixi746 – 775Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0BX-ray2.06A654-778[»]
1BDJX-ray2.68B654-778[»]
1FR0NMR-A654-778[»]
2A0BX-ray1.57A654-778[»]
2KSDNMR-A1-115[»]
ProteinModelPortaliP0AEC3.
SMRiP0AEC3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEC3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 223PASPROSITE-ProRule annotationAdd BLAST71
Domaini226 – 278PACPROSITE-ProRule annotationAdd BLAST53
Domaini289 – 507Histidine kinasePROSITE-ProRule annotationAdd BLAST219
Domaini527 – 643Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini678 – 771HPtPROSITE-ProRule annotationAdd BLAST94

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 HPt domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0642. LUCA.
COG0784. LUCA.
COG2198. LUCA.
HOGENOMiHOG000272667.
InParanoidiP0AEC3.
KOiK07648.
OMAiIGSSIMR.
PhylomeDBiP0AEC3.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.10.287.970. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR027460. ArcB_TM.
IPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014409. Sig_transdc_His_kin_hyb_ArcB.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF003182. ArcB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV
60 70 80 90 100
ESIDVIRSIF FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER
110 120 130 140 150
DLSLNVQLKD NIAQLNQEIA VREKAEAELQ ETFGQLKIEI KEREETQIQL
160 170 180 190 200
EQQSSFLRSF LDASPDLVFY RNEDKEFSGC NRAMELLTGK SEKQLVHLKP
210 220 230 240 250
ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA CFEIRKVPYY
260 270 280 290 300
DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG
310 320 330 340 350
IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ
360 370 380 390 400
LDNQPVDFTS FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR
410 420 430 440 450
QILWNLISNA VKFTQQGQVT VRVRYDEGDM LHFEVEDSGI GIPQDELDKI
460 470 480 490 500
FAMYYQVKDS HGGKPATGTG IGLAVSRRLA KNMGGDITVT SEQGKGSTFT
510 520 530 540 550
LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV ARSVLEKLGN
560 570 580 590 600
SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL
610 620 630 640 650
PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE
660 670 680 690 700
STVTTEENSK SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL
710 720 730 740 750
ESNLTAQDKK GIVEEGHKIK GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV
760 770
GEWIEEMKEE WRHDVEVLKA WVAKATKK
Length:778
Mass (Da):87,983
Last modified:December 6, 2005 - v1
Checksum:iDD61EA6ECF95AD30
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti469 – 470Missing in AAA58012 (PubMed:9278503).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53315 Genomic DNA. Translation: CAA37397.1.
U18997 Genomic DNA. Translation: AAA58012.1.
U00096 Genomic DNA. Translation: AAT48172.1.
AP009048 Genomic DNA. Translation: BAE77254.1.
PIRiD65112. RGECAR.
RefSeqiWP_000809774.1. NZ_LN832404.1.
YP_026207.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48172; AAT48172; b3210.
BAE77254; BAE77254; BAE77254.
GeneIDi947887.
KEGGiecj:JW5536.
eco:b3210.
PATRICi32121840. VBIEscCol129921_3304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53315 Genomic DNA. Translation: CAA37397.1.
U18997 Genomic DNA. Translation: AAA58012.1.
U00096 Genomic DNA. Translation: AAT48172.1.
AP009048 Genomic DNA. Translation: BAE77254.1.
PIRiD65112. RGECAR.
RefSeqiWP_000809774.1. NZ_LN832404.1.
YP_026207.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0BX-ray2.06A654-778[»]
1BDJX-ray2.68B654-778[»]
1FR0NMR-A654-778[»]
2A0BX-ray1.57A654-778[»]
2KSDNMR-A1-115[»]
ProteinModelPortaliP0AEC3.
SMRiP0AEC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263422. 370 interactors.
DIPiDIP-47915N.
IntActiP0AEC3. 7 interactors.
MINTiMINT-98513.
STRINGi511145.b3210.

PTM databases

iPTMnetiP0AEC3.

Proteomic databases

EPDiP0AEC3.
PaxDbiP0AEC3.
PRIDEiP0AEC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48172; AAT48172; b3210.
BAE77254; BAE77254; BAE77254.
GeneIDi947887.
KEGGiecj:JW5536.
eco:b3210.
PATRICi32121840. VBIEscCol129921_3304.

Organism-specific databases

EchoBASEiEB0060.
EcoGeneiEG10062. arcB.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0642. LUCA.
COG0784. LUCA.
COG2198. LUCA.
HOGENOMiHOG000272667.
InParanoidiP0AEC3.
KOiK07648.
OMAiIGSSIMR.
PhylomeDBiP0AEC3.

Enzyme and pathway databases

BioCyciEcoCyc:ARCB-MONOMER.
ECOL316407:JW5536-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AEC3.
PROiP0AEC3.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.10.287.970. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR027460. ArcB_TM.
IPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014409. Sig_transdc_His_kin_hyb_ArcB.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF003182. ArcB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARCB_ECOLI
AccessioniPrimary (citable) accession number: P0AEC3
Secondary accession number(s): P22763, Q2M902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.