ID CYSG_ECOLI Reviewed; 457 AA. AC P0AEA8; P11098; P76685; Q2M734; DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot. DT 06-DEC-2005, sequence version 1. DT 16-JUN-2009, entry version 39. DE RecName: Full=Siroheme synthase; DE Includes: DE RecName: Full=Uroporphyrinogen-III C-methyltransferase; DE Short=Urogen III methylase; DE EC=2.1.1.107; DE AltName: Full=SUMT; DE AltName: Full=Uroporphyrinogen III methylase; DE Short=UROM; DE Includes: DE RecName: Full=Precorrin-2 dehydrogenase; DE EC=1.3.1.76; DE Includes: DE RecName: Full=Sirohydrochlorin ferrochelatase; DE EC=4.99.1.4; GN Name=cysG; OrderedLocusNames=b3368, JW3331; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX MEDLINE=89282391; PubMed=2543955; DOI=10.1093/nar/17.10.3865; RA Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.; RT "Cloning of binding sequences for the Escherichia coli transcription RT activators, FNR and CRP: location of bases involved in discrimination RT between FNR and CRP."; RL Nucleic Acids Res. 17:3865-3874(1989). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX MEDLINE=90345936; PubMed=2200672; RX DOI=10.1111/j.1432-1033.1990.tb19125.x; RA Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S., RA Harborne N., Wootton J., Nicolson R., Cole J.A.; RT "Nucleotide sequence, organisation and structural analysis of the RT products of genes in the nirB-cysG region of the Escherichia coli K-12 RT chromosome."; RL Eur. J. Biochem. 191:315-323(1990). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [5] RP FUNCTION, AND PROTEIN SEQUENCE OF 1-18. RX MEDLINE=90169130; PubMed=2407558; DOI=10.1016/0014-5793(90)80640-5; RA Warren M.J., Stolowich N.J., Santander P.J., Roessner C.A., Sowa B.A., RA Scott A.I.; RT "Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a RT novel pyrrocorphin by uroporphyrinogen III methylase."; RL FEBS Lett. 261:76-80(1990). RN [6] RP FUNCTION. RX MEDLINE=90165870; PubMed=2407234; RA Warren M.J., Roessner C.A., Santander P.J., Scott A.I.; RT "The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent RT uroporphyrinogen III methylase."; RL Biochem. J. 265:725-729(1990). RN [7] RP FUNCTION. RX MEDLINE=94063079; PubMed=8243665; DOI=10.1016/0014-5793(93)80438-Z; RA Spencer J.B., Stolowich N.J., Roessner C.A., Scott A.I.; RT "The Escherichia coli cysG gene encodes the multifunctional protein, RT siroheme synthase."; RL FEBS Lett. 335:57-60(1993). CC -!- FUNCTION: Multifunctional enzyme that catalyzes the SAM-dependent CC methylation of uroporphyrinogen III at position C-2 and C-7 to CC form precorrin-2 and then position C-12 or C-18 to form CC trimethylpyrrocorphin 2. It also catalyzes the conversion of CC precorrin-2 into siroheme. This reaction consists of the NAD- CC dependent oxidation of precorrin-2 into sirohydrochlorin and its CC subsequent ferrochelation into siroheme. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + uroporphyrinogen III CC = S-adenosyl-L-homocysteine + precorrin-1. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + precorrin-1 = S- CC adenosyl-L-homocysteine + precorrin-2. CC -!- CATALYTIC ACTIVITY: Precorrin-2 + NAD(+) = sirohydrochlorin + CC NADH. CC -!- CATALYTIC ACTIVITY: Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+). CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; CC precorrin-2 from uroporphyrinogen III: step 1/1. CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; CC sirohydrochlorin from precorrin-2: step 1/1. CC -!- PATHWAY: Porphyrin metabolism; siroheme biosynthesis; precorrin-2 CC from uroporphyrinogen III: step 1/1. CC -!- PATHWAY: Porphyrin metabolism; siroheme biosynthesis; siroheme CC from sirohydrochlorin: step 1/1. CC -!- PATHWAY: Porphyrin metabolism; siroheme biosynthesis; CC sirohydrochlorin from precorrin-2: step 1/1. CC -!- INTERACTION: CC P18843:nadE; NbExp=1; IntAct=EBI-1132623, EBI-548960; CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; X14202; CAA32419.1; -; Genomic_DNA. DR EMBL; U18997; AAA58165.1; -; Genomic_DNA. DR EMBL; U00096; AAC76393.1; -; Genomic_DNA. DR EMBL; AP009048; BAE77922.1; -; Genomic_DNA. DR PIR; C65131; C65131. DR RefSeq; AP_004421.1; -. DR RefSeq; NP_417827.1; -. DR HSSP; O87696; 1CBF. DR SMR; P0AEA8; 1-457. DR IntAct; P0AEA8; 1. DR GeneID; 947880; -. DR GenomeReviews; AP009048_GR; JW3331. DR GenomeReviews; U00096_GR; b3368. DR KEGG; ecj:JW3331; -. DR KEGG; eco:b3368; -. DR EchoBASE; EB0185; -. DR EcoGene; EG10188; cysG. DR HOGENOM; P0AEA8; -. DR OMA; P0AEA8; MCRRDAE. DR BioCyc; EcoCyc:SIROHEMESYN-MON; -. DR BioCyc; MetaCyc:SIROHEMESYN-MON; -. DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:HAMAP. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:EC. DR GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:HAMAP. DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:HAMAP. DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW. DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:HAMAP. DR HAMAP; MF_01646; -; 1. DR InterPro; IPR000878; 4pyrrol_Mease. DR InterPro; IPR014777; 4pyrrole_Mease_sub1. DR InterPro; IPR014776; 4pyrrole_Mease_sub2. DR InterPro; IPR006366; CobA_cysG_C. DR InterPro; IPR016040; NAD(P)-bd_dom. DR InterPro; IPR019478; Sirohaem_synthase_dimer_dom. DR InterPro; IPR006367; Sirohaem_synthase_N. DR InterPro; IPR003043; Uropor_MeTrfase_CS. DR Gene3D; G3DSA:3.40.1010.10; 4pyrrole_Mease_sub1; 1. DR Gene3D; G3DSA:3.30.950.10; 4pyrrole_Mease_sub2; 1. DR Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1. DR Pfam; PF10414; CysG_dimeriser; 1. DR Pfam; PF00590; TP_methylase; 1. DR TIGRFAMs; TIGR01469; cobA_cysG_Cterm; 1. DR TIGRFAMs; TIGR01470; cysG_Nterm; 1. DR PROSITE; PS00839; SUMT_1; 1. DR PROSITE; PS00840; SUMT_2; 1. PE 1: Evidence at protein level; KW Cobalamin biosynthesis; Complete proteome; Direct protein sequencing; KW Lyase; Methyltransferase; Multifunctional enzyme; NAD; Oxidoreductase; KW Porphyrin biosynthesis; S-adenosyl-L-methionine; Transferase. FT CHAIN 1 457 Siroheme synthase. FT /FTId=PRO_0000150378. FT REGION 218 457 Uroporphyrinogen-III C-methyltransferase. FT CONFLICT 26 26 R -> P (in Ref. 1 and 2). FT CONFLICT 90 91 SE -> RQ (in Ref. 1 and 2). SQ SEQUENCE 457 AA; 49951 MW; DDDE0DC90C5CF2F4 CRC64; MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLDAGARLTV NALAFIPQFT AWADAGMLTL VEGPFDESLL DTCWLAIAAT DDDALNQRVS EAAEARRIFC NVVDAPKAAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR EKLESLLPLH LGQVAKYAGQ LRGRVKQQFA TMGERRRFWE KLFVNDRLAQ SLANNDQKAI TETTEQLINE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADVVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK GGDPFIFGRG GEELETLCNA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLITGHL KTGGELDWEN LAAEKQTLVF YMGLNQAATI QQKLIEHGMP GEMPVAIVEN GTAVTQRVID GTLTQLGELA QQMNSPSLII IGRVVGLRDK LNWFSNH //