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Protein

Protoheme IX farnesyltransferase

Gene

cyoE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.2 Publications

Pathwayi: heme O biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes heme O from protoheme.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoheme IX farnesyltransferase (cyoE)
This subpathway is part of the pathway heme O biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes heme O from protoheme, the pathway heme O biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • protoheme IX farnesyltransferase activity Source: EcoliWiki

GO - Biological processi

  • heme O biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Heme biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:HEMEOSYN-MONOMER.
ECOL316407:JW0418-MONOMER.
MetaCyc:HEMEOSYN-MONOMER.
BRENDAi2.5.1.B36. 2026.
UniPathwayiUPA00834; UER00712.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoheme IX farnesyltransferase (EC:2.5.1.-)
Alternative name(s):
Heme B farnesyltransferase
Heme O synthase
Gene namesi
Name:cyoE
Ordered Locus Names:b0428, JW0418
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10182. cyoE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicCurated
Transmembranei10 – 2819HelicalCuratedAdd
BLAST
Topological domaini29 – 379PeriplasmicCurated
Transmembranei38 – 5619HelicalCuratedAdd
BLAST
Topological domaini57 – 7822CytoplasmicCuratedAdd
BLAST
Transmembranei79 – 9719HelicalCuratedAdd
BLAST
Topological domaini98 – 10710PeriplasmicCurated
Transmembranei108 – 12619HelicalCuratedAdd
BLAST
Topological domaini127 – 19771CytoplasmicCuratedAdd
BLAST
Transmembranei198 – 21619HelicalCuratedAdd
BLAST
Topological domaini217 – 22812PeriplasmicCuratedAdd
BLAST
Transmembranei229 – 24719HelicalCuratedAdd
BLAST
Topological domaini248 – 26821CytoplasmicCuratedAdd
BLAST
Transmembranei269 – 28719HelicalCuratedAdd
BLAST
Topological domaini288 – 2969PeriplasmicCurated

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Protoheme IX farnesyltransferasePRO_0000162895Add
BLAST

Proteomic databases

PaxDbiP0AEA5.

Interactioni

Protein-protein interaction databases

BioGridi4259839. 2 interactions.
DIPiDIP-47946N.
STRINGi511145.b0428.

Structurei

3D structure databases

ProteinModelPortaliP0AEA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F1R. Bacteria.
COG0109. LUCA.
HOGENOMiHOG000237290.
InParanoidiP0AEA5.
KOiK02301.
OMAiIWWLGMA.
PhylomeDBiP0AEA5.

Family and domain databases

CDDicd13957. PT_UbiA_Cox10. 1 hit.
HAMAPiMF_00154. CyoE_CtaB. 1 hit.
InterProiIPR006369. Protohaem_IX_farnesylTrfase.
IPR000537. UbiA_prenyltransferase.
IPR030470. UbiA_prenylTrfase_CS.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01473. cyoE_ctaB. 1 hit.
PROSITEiPS00943. UBIA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFKQYLQVT KPGIIFGNLI SVIGGFLLAS KGSIDYPLFI YTLVGVSLVV
60 70 80 90 100
ASGCVFNNYI DRDIDRKMER TKNRVLVKGL ISPAVSLVYA TLLGIAGFML
110 120 130 140 150
LWFGANPLAC WLGVMGFVVY VGVYSLYMKR HSVYGTLIGS LSGAAPPVIG
160 170 180 190 200
YCAVTGEFDS GAAILLAIFS LWQMPHSYAI AIFRFKDYQA ANIPVLPVVK
210 220 230 240 250
GISVAKNHIT LYIIAFAVAT LMLSLGGYAG YKYLVVAAAV SVWWLGMALR
260 270 280 290
GYKVADDRIW ARKLFGFSII AITALSVMMS VDFMVPDSHT LLAAVW
Length:296
Mass (Da):32,248
Last modified:December 6, 2005 - v1
Checksum:iD3AFC612ECFB450E
GO

Sequence cautioni

The sequence AAB40184 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA. Translation: AAA23635.1.
U82664 Genomic DNA. Translation: AAB40184.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73531.1.
AP009048 Genomic DNA. Translation: BAE76208.1.
PIRiE42226.
RefSeqiNP_414962.1. NC_000913.3.
WP_000971336.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73531; AAC73531; b0428.
BAE76208; BAE76208; BAE76208.
GeneIDi945073.
KEGGiecj:JW0418.
eco:b0428.
PATRICi32116007. VBIEscCol129921_0445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA. Translation: AAA23635.1.
U82664 Genomic DNA. Translation: AAB40184.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73531.1.
AP009048 Genomic DNA. Translation: BAE76208.1.
PIRiE42226.
RefSeqiNP_414962.1. NC_000913.3.
WP_000971336.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259839. 2 interactions.
DIPiDIP-47946N.
STRINGi511145.b0428.

Proteomic databases

PaxDbiP0AEA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73531; AAC73531; b0428.
BAE76208; BAE76208; BAE76208.
GeneIDi945073.
KEGGiecj:JW0418.
eco:b0428.
PATRICi32116007. VBIEscCol129921_0445.

Organism-specific databases

EchoBASEiEB0179.
EcoGeneiEG10182. cyoE.

Phylogenomic databases

eggNOGiENOG4105F1R. Bacteria.
COG0109. LUCA.
HOGENOMiHOG000237290.
InParanoidiP0AEA5.
KOiK02301.
OMAiIWWLGMA.
PhylomeDBiP0AEA5.

Enzyme and pathway databases

UniPathwayiUPA00834; UER00712.
BioCyciEcoCyc:HEMEOSYN-MONOMER.
ECOL316407:JW0418-MONOMER.
MetaCyc:HEMEOSYN-MONOMER.
BRENDAi2.5.1.B36. 2026.

Miscellaneous databases

PROiP0AEA5.

Family and domain databases

CDDicd13957. PT_UbiA_Cox10. 1 hit.
HAMAPiMF_00154. CyoE_CtaB. 1 hit.
InterProiIPR006369. Protohaem_IX_farnesylTrfase.
IPR000537. UbiA_prenyltransferase.
IPR030470. UbiA_prenylTrfase_CS.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01473. cyoE_ctaB. 1 hit.
PROSITEiPS00943. UBIA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYOE_ECOLI
AccessioniPrimary (citable) accession number: P0AEA5
Secondary accession number(s): P18404, P77115, Q2MBZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.