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Protein

Transcriptional regulatory protein CpxR

Gene

cpxR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).3 Publications

Enzyme regulationi

The two-component system is activated by envelope stress such as overexpression of some (misfolded) periplasmic proteins (PubMed:7883164, PubMed:9351822). Activated by spheroplasting (which removes the periplasm) in an autoregulatory cpxA-cpxR-dependent fashion (PubMed:10972835). Cpx two-component system is activated at pH 8.0; in a degP deletion mutant activation is halved (PubMed:9473036, PubMed:16166523). The CpxA kinase activity is inhibited by periplasmic accessory protein CpxP; proteolysis of CpxP relieves inhibition (PubMed:16166523, PubMed:17259177, PubMed:25207645).5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi131 – 230100OmpR/PhoB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:CPXR-MONOMER.
ECOL316407:JW3883-MONOMER.
RETL1328306-WGS:GSTH-6652-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein CpxR
Gene namesi
Name:cpxR
Synonyms:yiiA
Ordered Locus Names:b3912, JW3883
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10020. cpxR.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of the Cpx envelope stress response (PubMed:10972835). A double cpxR-cpxA mutant decreases transcription of degP (PubMed:7883164). Cells are less sensitive to killing by nalidixic acid; double cpxR-srkA disruption mutants are as sensitive to killing as single srkA mutants, suggesting the SrkA protein kinase is partially regulated by CpxR. Hypersensitive to alkaline pH (greater than pH 8.8) (PubMed:9473036).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Transcriptional regulatory protein CpxRPRO_0000081078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 5114-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by CpxA.1 Publication2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AE88.
PaxDbiP0AE88.

Interactioni

Subunit structurei

Interacts with cognate sensor kinase CpxA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
lysAP008612EBI-550918,EBI-553837

Protein-protein interaction databases

BioGridi4262645. 289 interactions.
DIPiDIP-47991N.
IntActiP0AE88. 11 interactions.
MINTiMINT-1292432.
STRINGi511145.b3912.

Structurei

Secondary structure

1
232
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi11 – 2313Combined sources
Beta strandi27 – 337Combined sources
Helixi34 – 407Combined sources
Beta strandi47 – 504Combined sources
Beta strandi55 – 573Combined sources
Helixi59 – 668Combined sources
Turni67 – 693Combined sources
Beta strandi74 – 796Combined sources
Helixi83 – 919Combined sources
Beta strandi95 – 1017Combined sources
Helixi104 – 12320Combined sources
Beta strandi134 – 1363Combined sources
Beta strandi139 – 1424Combined sources
Turni143 – 1464Combined sources
Beta strandi147 – 1504Combined sources
Helixi159 – 17012Combined sources
Turni171 – 1733Combined sources
Helixi178 – 1869Combined sources
Helixi196 – 20712Combined sources
Beta strandi217 – 2215Combined sources
Turni222 – 2243Combined sources
Beta strandi225 – 2284Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UHJX-ray1.90A/B/C1-123[»]
4UHKX-ray2.60A/B/C1-123[»]
4UHSX-ray5.00A/B/C1-123[»]
4UHTX-ray1.15A/B131-231[»]
ProteinModelPortaliP0AE88.
SMRiP0AE88. Positions 3-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 115113Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105E3W. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP0AE88.
KOiK07662.
OMAiRRDGHPW.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0AE88.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AE88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILLVDDD RELTSLLKEL LEMEGFNVIV AHDGEQALDL LDDSIDLLLL
60 70 80 90 100
DVMMPKKNGI DTLKALRQTH QTPVIMLTAR GSELDRVLGL ELGADDYLPK
110 120 130 140 150
PFNDRELVAR IRAILRRSHW SEQQQNNDNG SPTLEVDALV LNPGRQEASF
160 170 180 190 200
DGQTLELTGT EFTLLYLLAQ HLGQVVSREH LSQEVLGKRL TPFDRAIDMH
210 220 230
ISNLRRKLPD RKDGHPWFKT LRGRGYLMVS AS
Length:232
Mass (Da):26,312
Last modified:December 6, 2005 - v1
Checksum:i667FC2B246082F46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14579 Unassigned DNA. Translation: AAC36868.1.
L19201 Genomic DNA. Translation: AAB03045.1.
U00096 Genomic DNA. Translation: AAC76894.1.
AP009048 Genomic DNA. Translation: BAE77397.1.
X13307 Genomic DNA. Translation: CAA31686.1.
M36795 Genomic DNA. Translation: AAA23599.1.
PIRiC65197.
RefSeqiNP_418348.1. NC_000913.3.
WP_001033722.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76894; AAC76894; b3912.
BAE77397; BAE77397; BAE77397.
GeneIDi948404.
KEGGiecj:JW3883.
eco:b3912.
PATRICi32123337. VBIEscCol129921_4029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14579 Unassigned DNA. Translation: AAC36868.1.
L19201 Genomic DNA. Translation: AAB03045.1.
U00096 Genomic DNA. Translation: AAC76894.1.
AP009048 Genomic DNA. Translation: BAE77397.1.
X13307 Genomic DNA. Translation: CAA31686.1.
M36795 Genomic DNA. Translation: AAA23599.1.
PIRiC65197.
RefSeqiNP_418348.1. NC_000913.3.
WP_001033722.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UHJX-ray1.90A/B/C1-123[»]
4UHKX-ray2.60A/B/C1-123[»]
4UHSX-ray5.00A/B/C1-123[»]
4UHTX-ray1.15A/B131-231[»]
ProteinModelPortaliP0AE88.
SMRiP0AE88. Positions 3-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262645. 289 interactions.
DIPiDIP-47991N.
IntActiP0AE88. 11 interactions.
MINTiMINT-1292432.
STRINGi511145.b3912.

Proteomic databases

EPDiP0AE88.
PaxDbiP0AE88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76894; AAC76894; b3912.
BAE77397; BAE77397; BAE77397.
GeneIDi948404.
KEGGiecj:JW3883.
eco:b3912.
PATRICi32123337. VBIEscCol129921_4029.

Organism-specific databases

EchoBASEiEB0019.
EcoGeneiEG10020. cpxR.

Phylogenomic databases

eggNOGiENOG4105E3W. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP0AE88.
KOiK07662.
OMAiRRDGHPW.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0AE88.

Enzyme and pathway databases

BioCyciEcoCyc:CPXR-MONOMER.
ECOL316407:JW3883-MONOMER.
RETL1328306-WGS:GSTH-6652-MONOMER.

Miscellaneous databases

PROiP0AE88.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The deduced amino-acid sequence of the cloned cpxR gene suggests the protein is the cognate regulator for the membrane sensor, CpxA, in a two-component signal transduction system of Escherichia coli."
    Dong J., Iuchi S., Kwan H.-S., Lu Z., Lin E.
    Gene 136:227-230(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: L547.
  2. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The cpx proteins of Escherichia coli K12. Structure of the cpxA polypeptide as an inner membrane component."
    Weber R.F., Silverman P.M.
    J. Mol. Biol. 203:467-478(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-232.
    Strain: K12.
  6. "The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP."
    Danese P.N., Snyder W.B., Cosma C.L., Davis L.J., Silhavy T.J.
    Genes Dev. 9:387-398(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRANSCRIPTIONAL ACTIVATOR, ENZYME REGULATION, PHOSPHORYLATION BY CPXA, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100.
  7. "The chaperone-assisted membrane release and folding pathway is sensed by two signal transduction systems."
    Jones C.H., Danese P.N., Pinkner J.S., Silhavy T.J., Hultgren S.J.
    EMBO J. 16:6394-6406(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  8. "Transduction of envelope stress in Escherichia coli by the Cpx two-component system."
    Raivio T.L., Silhavy T.J.
    J. Bacteriol. 179:7724-7733(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION BY CPXA, DNA-BINDING.
    Strain: K12 / MC4100.
  9. "CpxP, a stress-combative member of the Cpx regulon."
    Danese P.N., Silhavy T.J.
    J. Bacteriol. 180:831-839(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100.
  10. "Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response."
    Raivio T.L., Laird M.W., Joly J.C., Silhavy T.J.
    Mol. Microbiol. 37:1186-1197(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100.
  11. "Cpx signal transduction is influenced by a conserved N-terminal domain in the novel inhibitor CpxP and the periplasmic protease DegP."
    Buelow D.R., Raivio T.L.
    J. Bacteriol. 187:6622-6630(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
    Strain: K12 / MC4100.
  12. "Purification, reconstitution, and characterization of the CpxRAP envelope stress system of Escherichia coli."
    Fleischer R., Heermann R., Jung K., Hunke S.
    J. Biol. Chem. 282:8583-8593(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, PHOSPHORYLATION BY CPXA.
  13. "YihE kinase is a central regulator of programmed cell death in bacteria."
    Dorsey-Oresto A., Lu T., Mosel M., Wang X., Salz T., Drlica K., Zhao X.
    Cell Rep. 3:528-537(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  14. "Dynamic interaction between the CpxA sensor kinase and the periplasmic accessory protein CpxP mediates signal recognition in E. coli."
    Tschauner K., Hoernschemeyer P., Mueller V.S., Hunke S.
    PLoS ONE 9:E107383-E107383(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, INTERACTION WITH CPXA, SUBUNIT.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCPXR_ECOLI
AccessioniPrimary (citable) accession number: P0AE88
Secondary accession number(s): P16244, P76777, Q2M8K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.