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Reviewed, UniProtKB/Swiss-Prot P0AE82 (CPXA_ECOLI)

Last modified November 3, 2009. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sensor protein cpxA
    EC=2.7.13.3
Gene names
Name: cpxA
Synonyms: ecfB, eup, ssd
Ordered Locus Names: b3911, JW3882
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This protein is involved in several diverse cellular processes, such as the functioning of acetohydroxyacid synthetase I, in the biosynthesis of isoleucine and valine, the traJ protein activation activity for tra gene expression in F plasmid, and the synthesis, translocation, or stability of cell envelope proteins. Activates cpxR by phosphorylation.

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Contains 1 HAMP domain.

Contains 1 histidine kinase domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457Sensor protein cpxA
PRO_0000074739

Regions

Topological domain1 – 77Cytoplasmic Probable
Transmembrane8 – 2922 Probable
Topological domain30 – 163134Periplasmic Probable
Transmembrane164 – 18421 Probable
Topological domain185 – 457273Cytoplasmic Probable
Domain185 – 23753HAMP
Domain245 – 455211Histidine kinase

Amino acid modifications

Modified residue2481Phosphohistidine; by autocatalysis By similarity

Experimental info

Sequence conflict681W → WW in AAA23600. Ref.1
Sequence conflict681W → WW in CAA31687. Ref.2
Sequence conflict3301K → R in AAA23600. Ref.1
Sequence conflict3301K → R in AAA72540. Ref.1
Sequence conflict3301K → R in CAA31687. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P0AE82-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 720EE4A62885BA33

FASTA45751,624
        10         20         30         40         50         60 
MIGSLTARIF AIFWLTLALV LMLVLMLPKL DSRQMTELLD SEQRQGLMIE QHVEAELAND 

        70         80         90        100        110        120 
PPNDLMWWRR LFRAIDKWAP PGQRLLLVTT EGRVIGAERS EMQIIRNFIG QADNADHPQK 

       130        140        150        160        170        180 
KKYGRVELVG PFSVRDGEDN YQLYLIRPAS SSQSDFINLL FDRPLLLLIV TMLVSTPLLL 

       190        200        210        220        230        240 
WLAWSLAKPA RKLKNAADEV AQGNLRQHPE LEAGPQEFLA AGASFNQMVT ALERMMTSQQ 

       250        260        270        280        290        300 
RLLSDISHEL RTPLTRLQLG TALLRRRSGE SKELERIETE AQRLDSMIND LLVMSRNQQK 

       310        320        330        340        350        360 
NALVSETIKA NQLWSEVLDN AAFEAEQMGK SLTVNFPPGP WPLYGNPNAL ESALENIVRN 

       370        380        390        400        410        420 
ALRYSHTKIE VGFAVDKDGI TITVDDDGPG VSPEDREQIF RPFYRTDEAR DRESGGTGLG 

       430        440        450 
LAIVETAIQQ HRGWVKAEDS PLGGLRLVIW LPLYKRS

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References

« Hide 'large scale' references
[1]"The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product."
Albin R., Weber R.F., Silverman P.M.
J. Biol. Chem. 261:4698-4705(1986) [PubMed: 3007473] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The cpx proteins of Escherichia coli K12. Structure of the cpxA polypeptide as an inner membrane component."
Weber R.F., Silverman P.M.
J. Mol. Biol. 203:467-478(1988) [PubMed: 3058985] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The Cpx proteins of Escherichia coli K-12: evidence that cpxA, ecfB, ssd, and eup mutations all identify the same gene."
Rainwater S., Silverman P.M.
J. Bacteriol. 172:2456-2461(1990) [PubMed: 2185221] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases

M13493 Genomic DNA. Translation: AAA72540.1. Different initiation.
M36795 Genomic DNA. Translation: AAA23600.1.
X13307 Genomic DNA. Translation: CAA31687.1.
L19201 Genomic DNA. Translation: AAB03044.1.
U00096 Genomic DNA. Translation: AAC76893.1.
AP009048 Genomic DNA. Translation: BAE77398.1.
PIRS40855.
RefSeqAP_003897.1.
NP_418347.1.

3D structure databases

HSSPHSSP built from PDB template 1BXD based on UniProtKB P02933.
ModBaseSearch...

Protein-protein interaction databases

STRINGP0AE82.

Genome annotation databases

GeneID948405.
GenomeReviewsGene locus JW3882 in contig AP009048_GR.
Gene locus b3911 in contig U00096_GR.
KEGGecj:JW3882.
eco:b3911.

Organism-specific databases

EchoBASEEB0161.
EcoGeneEG10163. cpxA.
CMRSearch...

Phylogenomic databases

HOGENOMP0AE82.
OMASTNETKV.

Enzyme and pathway databases

BioCycEcoCyc:CPXA-MON.

Gene expression databases

GenevestigatorP0AE82.

Family and domain databases

InterProIPR003594. ATP_bd_ATPase.
IPR003660. HAMP_linker_domain.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
PfamPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSPR00344. BCTRLSENSOR.
SMARTSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
PROSITEPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCPXA_ECOLI
AccessionPrimary (citable) accession number: P0AE82
Secondary accession number(s): P08336, Q2M8K8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 6, 2005
Last modified: November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents