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Protein

Bifunctional adenosylcobalamin biosynthesis protein CobU

Gene

cobU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.By similarity

Catalytic activityi

ATP or GTP + adenosylcobinamide = adenosylcobinamide phosphate + ADP or GDP.By similarity
GTP + adenosylcobinamide phosphate = diphosphate + adenosylcobinamide-GDP.By similarity

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 5 and 6 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (btuR)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  6. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47GMP-histidine intermediateBy similarity1
Binding sitei59GTPBy similarity1
Binding sitei81GTP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 14GTPBy similarity8
Nucleotide bindingi31 – 33GTPBy similarity3
Nucleotide bindingi48 – 51GTPBy similarity4

GO - Molecular functioni

  • adenosylcobinamide kinase activity Source: UniProtKB-EC
  • ATP binding Source: UniProtKB-KW
  • cobinamide kinase activity Source: EcoCyc
  • cobinamide phosphate guanylyltransferase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:COBU-MONOMER
UniPathwayiUPA00148; UER00236
UPA00148; UER00237

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional adenosylcobalamin biosynthesis protein CobU
Alternative name(s):
Adenosylcobinamide kinase (EC:2.7.1.156By similarity)
Adenosylcobinamide-phosphate guanylyltransferase (EC:2.7.7.62By similarity)
Gene namesi
Name:cobU
Ordered Locus Names:b1993, JW1971
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13238 cobU

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899961 – 181Bifunctional adenosylcobalamin biosynthesis protein CobUAdd BLAST181

Proteomic databases

PaxDbiP0AE76
PRIDEiP0AE76

Expressioni

Inductioni

By cobinamide.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262116, 22 interactors
IntActiP0AE76, 4 interactors
STRINGi316385.ECDH10B_2137

Structurei

3D structure databases

ProteinModelPortaliP0AE76
SMRiP0AE76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobU/CobP family.Curated

Phylogenomic databases

eggNOGiENOG4108YZT Bacteria
COG2087 LUCA
HOGENOMiHOG000286613
InParanoidiP0AE76
KOiK02231
OMAiDCLTVWL
PhylomeDBiP0AE76

Family and domain databases

CDDicd00544 CobU, 1 hit
InterProiView protein in InterPro
IPR003203 Cobinamide_kinase/P_G-Trfase
IPR027417 P-loop_NTPase
PANTHERiPTHR34848 PTHR34848, 1 hit
PfamiView protein in Pfam
PF02283 CobU, 1 hit
PIRSFiPIRSF006135 CobU, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

P0AE76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMILVTGGAR SGKSRHAEAL IGDSSQVLYI ATSQILDDEM AARIEHHRQG
60 70 80 90 100
RPEHWRTVER WQHLDELIHA DINPNEVVLL ECVTTMVTNL LFDYGGDKDP
110 120 130 140 150
DEWDYQAMEQ AINAEIQSLI AACQRCPAKV VLVTNEVGMG IVPESRLARH
160 170 180
FRDIAGRVNQ QLAAAANEVW LVVSGIGVKI K
Length:181
Mass (Da):20,164
Last modified:December 6, 2005 - v1
Checksum:i072EB233416EBCB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA Translation: AAA78906.1
U00096 Genomic DNA Translation: AAC75054.1
AP009048 Genomic DNA Translation: BAA15810.1
PIRiH64963
RefSeqiNP_416497.1, NC_000913.3
WP_000973176.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75054; AAC75054; b1993
BAA15810; BAA15810; BAA15810
GeneIDi946519
KEGGiecj:JW1971
eco:b1993
PATRICifig|511145.12.peg.2068

Similar proteinsi

Entry informationi

Entry nameiCOBU_ECOLI
AccessioniPrimary (citable) accession number: P0AE76
Secondary accession number(s): P46886
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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