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Protein

Bifunctional adenosylcobalamin biosynthesis protein CobU

Gene

cobU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.By similarity

Catalytic activityi

ATP or GTP + adenosylcobinamide = adenosylcobinamide phosphate + ADP or GDP.By similarity
GTP + adenosylcobinamide phosphate = diphosphate + adenosylcobinamide-GDP.By similarity

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 5 and 6 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (btuR)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  6. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47GMP-histidine intermediateBy similarity1
Binding sitei59GTPBy similarity1
Binding sitei81GTP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 14GTPBy similarity8
Nucleotide bindingi31 – 33GTPBy similarity3
Nucleotide bindingi48 – 51GTPBy similarity4

GO - Molecular functioni

  • adenosylcobinamide kinase activity Source: UniProtKB-EC
  • ATP binding Source: UniProtKB-KW
  • cobinamide kinase activity Source: EcoCyc
  • cobinamide phosphate guanylyltransferase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:COBU-MONOMER.
ECOL316407:JW1971-MONOMER.
UniPathwayiUPA00148; UER00236.
UPA00148; UER00237.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional adenosylcobalamin biosynthesis protein CobU
Alternative name(s):
Adenosylcobinamide kinase (EC:2.7.1.156By similarity)
Adenosylcobinamide-phosphate guanylyltransferase (EC:2.7.7.62By similarity)
Gene namesi
Name:cobU
Ordered Locus Names:b1993, JW1971
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13238. cobU.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899961 – 181Bifunctional adenosylcobalamin biosynthesis protein CobUAdd BLAST181

Proteomic databases

PaxDbiP0AE76.
PRIDEiP0AE76.

Expressioni

Inductioni

By cobinamide.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262116. 18 interactors.
IntActiP0AE76. 4 interactors.
STRINGi511145.b1993.

Structurei

3D structure databases

ProteinModelPortaliP0AE76.
SMRiP0AE76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobU/CobP family.Curated

Phylogenomic databases

eggNOGiENOG4108YZT. Bacteria.
COG2087. LUCA.
HOGENOMiHOG000286613.
InParanoidiP0AE76.
KOiK02231.
OMAiLWLTNHL.
PhylomeDBiP0AE76.

Family and domain databases

CDDicd00544. CobU. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003203. Cobinamide_kinase/P_G-Trfase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02283. CobU. 1 hit.
[Graphical view]
PIRSFiPIRSF006135. CobU. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AE76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMILVTGGAR SGKSRHAEAL IGDSSQVLYI ATSQILDDEM AARIEHHRQG
60 70 80 90 100
RPEHWRTVER WQHLDELIHA DINPNEVVLL ECVTTMVTNL LFDYGGDKDP
110 120 130 140 150
DEWDYQAMEQ AINAEIQSLI AACQRCPAKV VLVTNEVGMG IVPESRLARH
160 170 180
FRDIAGRVNQ QLAAAANEVW LVVSGIGVKI K
Length:181
Mass (Da):20,164
Last modified:December 6, 2005 - v1
Checksum:i072EB233416EBCB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78906.1.
U00096 Genomic DNA. Translation: AAC75054.1.
AP009048 Genomic DNA. Translation: BAA15810.1.
PIRiH64963.
RefSeqiNP_416497.1. NC_000913.3.
WP_000973176.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75054; AAC75054; b1993.
BAA15810; BAA15810; BAA15810.
GeneIDi946519.
KEGGiecj:JW1971.
eco:b1993.
PATRICi32119317. VBIEscCol129921_2068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78906.1.
U00096 Genomic DNA. Translation: AAC75054.1.
AP009048 Genomic DNA. Translation: BAA15810.1.
PIRiH64963.
RefSeqiNP_416497.1. NC_000913.3.
WP_000973176.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AE76.
SMRiP0AE76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262116. 18 interactors.
IntActiP0AE76. 4 interactors.
STRINGi511145.b1993.

Proteomic databases

PaxDbiP0AE76.
PRIDEiP0AE76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75054; AAC75054; b1993.
BAA15810; BAA15810; BAA15810.
GeneIDi946519.
KEGGiecj:JW1971.
eco:b1993.
PATRICi32119317. VBIEscCol129921_2068.

Organism-specific databases

EchoBASEiEB3027.
EcoGeneiEG13238. cobU.

Phylogenomic databases

eggNOGiENOG4108YZT. Bacteria.
COG2087. LUCA.
HOGENOMiHOG000286613.
InParanoidiP0AE76.
KOiK02231.
OMAiLWLTNHL.
PhylomeDBiP0AE76.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00236.
UPA00148; UER00237.
BioCyciEcoCyc:COBU-MONOMER.
ECOL316407:JW1971-MONOMER.

Miscellaneous databases

PROiP0AE76.

Family and domain databases

CDDicd00544. CobU. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003203. Cobinamide_kinase/P_G-Trfase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02283. CobU. 1 hit.
[Graphical view]
PIRSFiPIRSF006135. CobU. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBU_ECOLI
AccessioniPrimary (citable) accession number: P0AE76
Secondary accession number(s): P46886
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.