PemK-like protein 1 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0AE70 (CHPA_ECOLI)

Last modified June 16, 2009. Version 30. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
PemK-like protein 1
Alternative name(s):
Protein mazF

Gene names

Name:	chpA
Synonyms:	chpAK, mazF
Ordered Locus Names:	b2782, JW2753

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the regulation of cell growth. It acts as a growth inhibitor. Both chpR and chpA bind to the promoter region of the chpRA operon to autoregulate their synthesis.
Sequence similarities	Belongs to the pemK family.

Ontologies

Keywords
Ligand	DNA-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yibL	P0ADK8	1	EBI-1036253,EBI-549687

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

111

PemK-like protein 1

PRO_0000201897

Secondary structure

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111

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

P0AE70-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 1579C867DD6B96AC
Show »

111

12,098

        10         20         30         40         50         60 
MVSRYVPDMG DLIWVDFDPT KGSEQAGHRP AVVLSPFMYN NKTGMCLCVP CTTQSKGYPF 

        70         80         90        100        110 
EVVLSGQERD GVALADQVKS IAWRARGATK KGTVAPEELQ LIKAKINVLI G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E. J. Bacteriol. 175:6850-6856(1993) [PubMed: 8226627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	Aizenman E., Glaser G. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

D16450 Genomic DNA. Translation: BAA03918.1.
J04039 Unassigned DNA. Translation: AAA03239.1.
U29580 Genomic DNA. Translation: AAA69292.1.
U00096 Genomic DNA. Translation: AAC75824.1.
AP009048 Genomic DNA. Translation: BAE76856.1.

PIR

RefSeq

AP_003348.1.
NP_417262.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UB4	X-ray	1.70	A/B	2-111	[»]

ModBase

Protein-protein interaction databases

IntAct

P0AE70. 2 interactions.

Genome annotation databases

GeneID

GenomeReviews

Gene locus JW2753 in contig AP009048_GR.
Gene locus b2782 in contig U00096_GR.

KEGG

ecj:JW2753.
eco:b2782.

Organism-specific databases

EchoBASE

EcoGene

EG11249. chpA.

CMR

Phylogenomic databases

HOGENOM

OMA

P0AE70. SGHEQAG.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11249-MON.

Family and domain databases

InterPro

IPR003477. PemK.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]

Gene3D

G3DSA:2.30.30.110. CcdB. 1 hit.

Pfam

PF02452. PemK. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

CHPA_ECOLI

Accession

Primary (citable) accession number: P0AE70
Secondary accession number(s): P33645, Q2MA50

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents