P0AE68 (CHEY_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis protein CheY

Gene names

Name:	cheY
Ordered Locus Names:	Z2936, ECs2592

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation By similarity.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Phosphorylated by CheA or acetylated by acetyl-CoA synthetase, depending on which acetate metabolism pathway is available By similarity.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis Flagellar rotation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW ciliary or flagellar motility Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 129	128	Chemotaxis protein CheY		PRO_0000081041
Regions
	Domain	7 – 124	118	Response regulatory
Sites
	Metal binding	12	1	Magnesium By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	57	1	Magnesium By similarity
	Metal binding	59	1	Magnesium; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity
	Modified residue	92	1	N6-acetyllysine By similarity
	Modified residue	109	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AE68 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E4B60B8A73DA14DC	FASTA	129	14,097
	10 20 30 40 50 60 MADKELKFLV VDDFSTMRRI VRNLLKELGF NNVEEAEDGV DALNKLQAGG YGFVISDWNM 70 80 90 100 110 120 PNMDGLELLK TIRADGAMSA LPVLMVTAEA KKENIIAAAQ AGASGYVVKP FTAATLEEKL NKIFEKLGM

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56872.1. BA000007 Genomic DNA. Translation: BAB36015.1.
PIR	D85801. H90952.
RefSeq	NP_288319.1. NC_002655.2. NP_310619.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AE68.
SMR	P0AE68. Positions 2-129.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-98475.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000024252; EBESCP00000023145; EBESCG00000023306. EBESCT00000059093; EBESCP00000056921; EBESCG00000058141.
GeneID	914203. 961854.
GenomeReviews	Gene locus Z2936 in contig AE005174_GR. Gene locus ECs2592 in contig BA000007_GR.
KEGG	ece:Z2936. ecs:ECs2592.
PATRIC	18354562. VBIEscCol44059_2492.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008654.
HOGENOM	HBG753323.
OMA	GFTNTSE.
ProtClustDB	PRK10610.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2592-MONOMER.
Family and domain databases
InterPro	IPR011006. CheY-like_superfamily. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
KO	K03413.
Pfam	PF00072. Response_reg. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEY_ECO57

Accession

Primary (citable) accession number: P0AE68
Secondary accession number(s): P06143

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program