P0AE52 (BCP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative peroxiredoxin bcp
EC=1.11.1.15

Alternative name(s):
Bacterioferritin comigratory protein
Thioredoxin reductase

Gene names

Name:	bcp
Ordered Locus Names:	b2480, JW2465

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Sequence similarities	Belongs to the AhpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Domain	Redox-active center
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to oxidative stress Inferred from mutant phenotype Ref.8. Source: EcoCyc
Molecular_function	hydroperoxide reductase activity Inferred from direct assay Ref.8. Source: EcoCyc thioredoxin peroxidase activity Inferred from direct assay Ref.8. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Putative peroxiredoxin bcp		PRO_0000135134
Regions
	Domain	4 – 156	153	Thioredoxin
Sites
	Active site	46	1	Cysteine sulfenic acid (-SOH) intermediate Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P0AE52 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C7D267A671409EFC	FASTA	156	17,634
	10 20 30 40 50 60 MNPLKAGDIA PKFSLPDQDG EQVNLTDFQG QRVLVYFYPK AMTPGCTVQA CGLRDNMDEL 70 80 90 100 110 120 KKAGVDVLGI STDKPEKLSR FAEKELLNFT LLSDEDHQVC EQFGVWGEKS FMGKTYDGIH 130 140 150 RISFLIDADG KIEHVFDDFK TSNHHDVVLN WLKEHA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map." Andrews S.C., Harrison P.M., Guest J.R. J. Gen. Microbiol. 137:361-367(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Promoter characterization and constitutive expression of the Escherichia coli gcvR gene." Ghrist A.C., Stauffer G.V. J. Bacteriol. 180:1803-1807(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15. Strain: K12 / EMG2.
[7]	Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F. Submitted (FEB-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Thioredoxin-dependent hydroperoxide peroxidase activity of bacterioferritin comigratory protein (BCP) as a new member of the thiol-specific antioxidant protein (TSA)/Alkyl hydroperoxide peroxidase C (AhpC) family." Jeong W., Cha M.K., Kim I.H. J. Biol. Chem. 275:2924-2930(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVE SITE CYS-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63654 Genomic DNA. Translation: AAB88562.1. U00096 Genomic DNA. Translation: AAC75533.1. AP009048 Genomic DNA. Translation: BAA16358.1. AF023337 Genomic DNA. Translation: AAC46233.1.
PIR	B49749.
RefSeq	NP_416975.1. NC_000913.2. YP_490708.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AE52.
SMR	P0AE52. Positions 1-154.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AE52. 6 interactions.
STRING	511145.b2480.
2D gel databases
SWISS-2DPAGE	P0AE52.
Proteomic databases
PaxDb	P0AE52.
PRIDE	P0AE52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75533; AAC75533; b2480. BAA16358; BAA16358; BAA16358.
GeneID	12931774. 946949.
KEGG	ecj:Y75_p2433. eco:b2480.
PATRIC	32120347. VBIEscCol129921_2576.
Organism-specific databases
EchoBASE	EB0106.
EcoGene	EG10108. bcp.
Phylogenomic databases
eggNOG	COG1225.
HOGENOM	HOG000022344.
KO	K03564.
OMA	NHHDIVL.
ProtClustDB	PRK09437.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10108-MONOMER. ECOL316407:JW2465-MONOMER. MetaCyc:EG10108-MONOMER.
Gene expression databases
Genevestigator	P0AE52.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000866. AhpC/TSA. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BCP_ECOLI

Accession

Primary (citable) accession number: P0AE52
Secondary accession number(s): P23480

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program