Reviewed, UniProtKB/Swiss-Prot P0AE52 (BCP_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative peroxiredoxin bcp
    EC=1.11.1.15
Alternative name(s):
    Thioredoxin reductase
    Bacterioferritin comigratory protein

Gene names

Name:	bcp
Ordered Locus Names:	b2480, JW2465

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Domain	Redox-active center
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Putative peroxiredoxin bcp		PRO_0000135134
Regions
	Domain	4 – 156	153	Thioredoxin
Sites
	Active site	46	1	Cysteine sulfenic acid (-SOH) intermediate

Sequences

	Sequence		Length	Mass (Da)
	P0AE52-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C7D267A671409EFC	FASTA	156	17,634
	10 20 30 40 50 60 MNPLKAGDIA PKFSLPDQDG EQVNLTDFQG QRVLVYFYPK AMTPGCTVQA CGLRDNMDEL 70 80 90 100 110 120 KKAGVDVLGI STDKPEKLSR FAEKELLNFT LLSDEDHQVC EQFGVWGEKS FMGKTYDGIH 130 140 150 RISFLIDADG KIEHVFDDFK TSNHHDVVLN WLKEHA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map." Andrews S.C., Harrison P.M., Guest J.R. J. Gen. Microbiol. 137:361-367(1991) [PubMed: 2016588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Promoter characterization and constitutive expression of the Escherichia coli gcvR gene." Ghrist A.C., Stauffer G.V. J. Bacteriol. 180:1803-1807(1998) [PubMed: 9537378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15. Strain: K12 / EMG2.
[7]	Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F. Submitted (FEB-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Thioredoxin-dependent hydroperoxide peroxidase activity of bacterioferritin comigratory protein (BCP) as a new member of the thiol-specific antioxidant protein (TSA)/Alkyl hydroperoxide peroxidase C (AhpC) family." Jeong W., Cha M.K., Kim I.H. J. Biol. Chem. 275:2924-2930(2000) [PubMed: 10644761] [Abstract] Cited for: ACTIVE SITE CYS-46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63654 Genomic DNA. Translation: AAB88562.1. U00096 Genomic DNA. Translation: AAC75533.1. AP009048 Genomic DNA. Translation: BAA16358.1. AF023337 Genomic DNA. Translation: AAC46233.1.
PIR	B49749.
RefSeq	AP_003066.1. NP_416975.1.
3D structure databases
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P0AE52.
Genome annotation databases
GeneID	946949.
GenomeReviews	Gene locus b2480 in contig U00096_GR. Gene locus JW2465 in contig AP009048_GR.
KEGG	ecj:JW2465. eco:b2480.
Organism-specific databases
EchoBASE	EB0106.
EcoGene	EG10108. bcp.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AE52.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10108-MON. MetaCyc:EG10108-MON.
Family and domain databases
InterPro	IPR000866. AhpC-TSA. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BCP_ECOLI

Accession

Primary (citable) accession number: P0AE52
Secondary accession number(s): P23480

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)