Reviewed, UniProtKB/Swiss-Prot P0AE38 (ASTA_ECO57)

Names and origin

Protein names

Recommended name:
    Arginine N-succinyltransferase
      Short name=AST
    EC=2.3.1.109
Alternative name(s):
    AOST

Gene names

Name:	astA
Ordered Locus Names:	Z2779, ECs2453

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine By similarity.
Catalytic activity	Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine. HAMAP MF_01171
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 1/5. HAMAP MF_01171
Sequence similarities	Belongs to the arginine N-succinyltransferase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	arginine N-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Arginine N-succinyltransferase HAMAP MF_01171		PRO_0000064713
Sites
	Active site	229	1	Proton donor Potential
	Binding site	125	1	Succinyl-CoA; via amide nitrogen Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AE38-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 89C7BB412A880BEF	FASTA	344	38,456
	10 20 30 40 50 60 MMVIRPVERS DVSALMQLAS KTGGGLTSLP ANEATLSARI ERAIKTWQGE LPKSEQGYVF 70 80 90 100 110 120 VLEDSETGTV AGICAIEVAV GLNDPWYNYR VGTLVHASKE LNVYNALPTL FLSNDHTGSS 130 140 150 160 170 180 ELCTLFLDPD WRKEGNGYLL SKSRFMFMAA FRDKFNDKVV AEMRGVIDEH GYSPFWQSLG 190 200 210 220 230 240 KRFFSMDFSR ADFLCGTGQK AFIAELMPKH PIYTHFLSQE AQDVIGQVHP QTAPARAVLE 250 260 270 280 290 300 KEGFRYRNYI DIFDGGPTLE CDIDRVRAIR KSRLVEVAEG QPAQGDFPAC LVANENYHHF 310 320 330 340 RVVLVRTDPA TERLILTAAQ LDALKCHAGD RVRLVRLCAE EKTA

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG56733.1. BA000007 Genomic DNA. Translation: BAB35876.1.
PIR	A85784. E90935.
RefSeq	NP_288180.1. NP_310480.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	913079. 961718.
GenomeReviews	Gene locus Z2779 in contig AE005174_GR. Gene locus ECs2453 in contig BA000007_GR.
KEGG	ece:Z2779. ecs:ECs2453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AE38.
OMA	P0AE38. TLFLAND.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2453-MON.
Family and domain databases
HAMAP	MF_01171. [Tree]
InterPro	IPR007041. Arg_N-succinylTrfase_Ast/AOST. IPR017650. Arginine_N-succinylTrfase_AstA. [Graphical view]
Pfam	PF04958. AstA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03243. arg_catab_AOST. 1 hit. TIGR03244. arg_catab_AstA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTA_ECO57

Accession

Primary (citable) accession number: P0AE38
Secondary accession number(s): P76218

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)