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Protein

Arginine N-succinyltransferase

Gene

astA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine.1 Publication

Catalytic activityi

Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Succinyl-CoA; via amide nitrogenSequence Analysis
Active sitei229 – 2291Proton donorSequence Analysis

GO - Molecular functioni

  1. arginine N-succinyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine catabolic process to glutamate Source: UniProtKB-HAMAP
  2. arginine catabolic process to succinate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Arginine metabolism, Stress response

Enzyme and pathway databases

BioCyciEcoCyc:ARGSUCCTRAN-MONOMER.
ECOL316407:JW1736-MONOMER.
MetaCyc:ARGSUCCTRAN-MONOMER.
UniPathwayiUPA00185; UER00279.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine N-succinyltransferase (EC:2.3.1.109)
Short name:
AST
Alternative name(s):
AOST
Gene namesi
Name:astA
Synonyms:ydjV
Ordered Locus Names:b1747, JW1736
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13998. astA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Arginine N-succinyltransferasePRO_0000064712Add
BLAST

Proteomic databases

PRIDEiP0AE37.

Expressioni

Inductioni

By nitrogen starvation, and arginine. Induced at stationary phase by sigma S.1 Publication

Gene expression databases

GenevestigatoriP0AE37.

Interactioni

Protein-protein interaction databases

IntActiP0AE37. 6 interactions.
STRINGi511145.b1747.

Structurei

3D structure databases

ProteinModelPortaliP0AE37.
SMRiP0AE37. Positions 1-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3138.
HOGENOMiHOG000258231.
InParanoidiP0AE37.
KOiK00673.
OMAiPVLECHI.
OrthoDBiEOG6XQ3H4.
PhylomeDBiP0AE37.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
HAMAPiMF_01171. AstA.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007041. Arg_N-succinylTrfase_Ast/AOST.
IPR017650. Arginine_N-succinylTrfase_AstA.
[Graphical view]
PfamiPF04958. AstA. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03243. arg_catab_AOST. 1 hit.
TIGR03244. arg_catab_AstA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AE37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVIRPVERS DVSALMQLAS KTGGGLTSLP ANEATLSARI ERAIKTWQGE
60 70 80 90 100
LPKSEQGYVF VLEDSETGTV AGICAIEVAV GLNDPWYNYR VGTLVHASKE
110 120 130 140 150
LNVYNALPTL FLSNDHTGSS ELCTLFLDPD WRKEGNGYLL SKSRFMFMAA
160 170 180 190 200
FRDKFNDKVV AEMRGVIDEH GYSPFWQSLG KRFFSMDFSR ADFLCGTGQK
210 220 230 240 250
AFIAELMPKH PIYTHFLSQE AQDVIGQVHP QTAPARAVLE KEGFRYRNYI
260 270 280 290 300
DIFDGGPTLE CDIDRVRAIR KSRLVEVAEG QPAQGDFPAC LVANENYHHF
310 320 330 340
RVVLVRTDPA TERLILTAAQ LDALKCHAGD RVRLVRLCAE EKTA
Length:344
Mass (Da):38,456
Last modified:December 5, 2005 - v1
Checksum:i89C7BB412A880BEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74817.1.
AP009048 Genomic DNA. Translation: BAE76517.1.
PIRiC64934.
RefSeqiNP_416261.1. NC_000913.3.
YP_490008.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74817; AAC74817; b1747.
BAE76517; BAE76517; BAE76517.
GeneIDi12933241.
946261.
KEGGiecj:Y75_p1722.
eco:b1747.
PATRICi32118803. VBIEscCol129921_1819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74817.1.
AP009048 Genomic DNA. Translation: BAE76517.1.
PIRiC64934.
RefSeqiNP_416261.1. NC_000913.3.
YP_490008.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0AE37.
SMRiP0AE37. Positions 1-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0AE37. 6 interactions.
STRINGi511145.b1747.

Proteomic databases

PRIDEiP0AE37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74817; AAC74817; b1747.
BAE76517; BAE76517; BAE76517.
GeneIDi12933241.
946261.
KEGGiecj:Y75_p1722.
eco:b1747.
PATRICi32118803. VBIEscCol129921_1819.

Organism-specific databases

EchoBASEiEB3754.
EcoGeneiEG13998. astA.

Phylogenomic databases

eggNOGiCOG3138.
HOGENOMiHOG000258231.
InParanoidiP0AE37.
KOiK00673.
OMAiPVLECHI.
OrthoDBiEOG6XQ3H4.
PhylomeDBiP0AE37.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00279.
BioCyciEcoCyc:ARGSUCCTRAN-MONOMER.
ECOL316407:JW1736-MONOMER.
MetaCyc:ARGSUCCTRAN-MONOMER.

Miscellaneous databases

PROiP0AE37.

Gene expression databases

GenevestigatoriP0AE37.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
HAMAPiMF_01171. AstA.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007041. Arg_N-succinylTrfase_Ast/AOST.
IPR017650. Arginine_N-succinylTrfase_AstA.
[Graphical view]
PfamiPF04958. AstA. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03243. arg_catab_AOST. 1 hit.
TIGR03244. arg_catab_AstA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli."
    Schneider B.L., Kiupakis A.K., Reitzer L.J.
    J. Bacteriol. 180:4278-4286(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli."
    Kiupakis A.K., Reitzer L.
    J. Bacteriol. 184:2940-2950(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Prediction of the structure and function of AstA and AstB, the first two enzymes of the arginine succinyltransferase pathway of arginine catabolism."
    Shirai H., Mizuguchi K.
    FEBS Lett. 555:505-510(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, REACTION MECHANISM.

Entry informationi

Entry nameiASTA_ECOLI
AccessioniPrimary (citable) accession number: P0AE37
Secondary accession number(s): P76218, Q2MB39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2005
Last sequence update: December 5, 2005
Last modified: January 6, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.