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Protein

Arabinose-proton symporter

Gene

araE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of arabinose across the boundary membrane with the concomitant transport of protons into the cell (symport system).

GO - Molecular functioni

  • fucose transmembrane transporter activity Source: EcoCyc
  • L-arabinose transmembrane transporter activity Source: EcoCyc
  • sugar:proton symporter activity Source: GO_Central
  • symporter activity Source: EcoCyc

GO - Biological processi

  • fucose transport Source: EcoCyc
  • L-arabinose transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:ARAE-MONOMER.
ECOL316407:JW2809-MONOMER.
MetaCyc:ARAE-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.2. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinose-proton symporter
Alternative name(s):
Arabinose transporter
Gene namesi
Name:araE
Ordered Locus Names:b2841, JW2809
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10056. araE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicSequence analysisAdd
BLAST
Transmembranei30 – 5021Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini51 – 6313PeriplasmicSequence analysisAdd
BLAST
Transmembranei64 – 8421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini85 – 917CytoplasmicSequence analysis
Transmembranei92 – 11221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini113 – 1142PeriplasmicSequence analysis
Transmembranei115 – 13521Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini136 – 15419CytoplasmicSequence analysisAdd
BLAST
Transmembranei155 – 17521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini176 – 1783PeriplasmicSequence analysis
Transmembranei179 – 19921Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini200 – 25758CytoplasmicSequence analysisAdd
BLAST
Transmembranei258 – 27821Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini279 – 29719PeriplasmicSequence analysisAdd
BLAST
Transmembranei298 – 31821Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini319 – 3257CytoplasmicSequence analysis
Transmembranei326 – 34621Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini347 – 36115PeriplasmicSequence analysisAdd
BLAST
Transmembranei362 – 38221Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini383 – 40422CytoplasmicSequence analysisAdd
BLAST
Transmembranei405 – 42521Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini426 – 4272PeriplasmicSequence analysis
Transmembranei428 – 44619Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini447 – 47226CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Arabinose-proton symporterPRO_0000050289Add
BLAST

Proteomic databases

PaxDbiP0AE24.
PRIDEiP0AE24.

Interactioni

Protein-protein interaction databases

BioGridi4259474. 16 interactions.
STRINGi511145.b2841.

Structurei

3D structure databases

ProteinModelPortaliP0AE24.
SMRiP0AE24. Positions 39-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QNK. Bacteria.
ENOG410XNQK. LUCA.
HOGENOMiHOG000202868.
InParanoidiP0AE24.
KOiK02100.
OMAiQIETDMA.
OrthoDBiEOG65J51S.
PhylomeDBiP0AE24.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AE24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTINTESAL TPRSLRDTRR MNMFVSVAAA VAGLLFGLDI GVIAGALPFI
60 70 80 90 100
TDHFVLTSRL QEWVVSSMML GAAIGALFNG WLSFRLGRKY SLMAGAILFV
110 120 130 140 150
LGSIGSAFAT SVEMLIAARV VLGIAVGIAS YTAPLYLSEM ASENVRGKMI
160 170 180 190 200
SMYQLMVTLG IVLAFLSDTA FSYSGNWRAM LGVLALPAVL LIILVVFLPN
210 220 230 240 250
SPRWLAEKGR HIEAEEVLRM LRDTSEKARE ELNEIRESLK LKQGGWALFK
260 270 280 290 300
INRNVRRAVF LGMLLQAMQQ FTGMNIIMYY APRIFKMAGF TTTEQQMIAT
310 320 330 340 350
LVVGLTFMFA TFIAVFTVDK AGRKPALKIG FSVMALGTLV LGYCLMQFDN
360 370 380 390 400
GTASSGLSWL SVGMTMMCIA GYAMSAAPVV WILCSEIQPL KCRDFGITCS
410 420 430 440 450
TTTNWVSNMI IGATFLTLLD SIGAAGTFWL YTALNIAFVG ITFWLIPETK
460 470
NVTLEHIERK LMAGEKLRNI GV
Length:472
Mass (Da):51,684
Last modified:December 6, 2005 - v1
Checksum:i411990A441D44393
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 283SVA → YDR in CAA25075 (PubMed:6319708).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03732 Genomic DNA. Translation: AAA23469.1.
U29581 Genomic DNA. Translation: AAB40488.1.
X00272 Genomic DNA. Translation: CAA25075.1.
U00096 Genomic DNA. Translation: AAC75880.1.
AP009048 Genomic DNA. Translation: BAE76910.1.
PIRiB26430.
RefSeqiNP_417318.1. NC_000913.3.
WP_000256438.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75880; AAC75880; b2841.
BAE76910; BAE76910; BAE76910.
GeneIDi947341.
KEGGiecj:JW2809.
eco:b2841.
PATRICi32121100. VBIEscCol129921_2939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03732 Genomic DNA. Translation: AAA23469.1.
U29581 Genomic DNA. Translation: AAB40488.1.
X00272 Genomic DNA. Translation: CAA25075.1.
U00096 Genomic DNA. Translation: AAC75880.1.
AP009048 Genomic DNA. Translation: BAE76910.1.
PIRiB26430.
RefSeqiNP_417318.1. NC_000913.3.
WP_000256438.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AE24.
SMRiP0AE24. Positions 39-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259474. 16 interactions.
STRINGi511145.b2841.

Protein family/group databases

TCDBi2.A.1.1.2. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP0AE24.
PRIDEiP0AE24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75880; AAC75880; b2841.
BAE76910; BAE76910; BAE76910.
GeneIDi947341.
KEGGiecj:JW2809.
eco:b2841.
PATRICi32121100. VBIEscCol129921_2939.

Organism-specific databases

EchoBASEiEB0054.
EcoGeneiEG10056. araE.

Phylogenomic databases

eggNOGiENOG4107QNK. Bacteria.
ENOG410XNQK. LUCA.
HOGENOMiHOG000202868.
InParanoidiP0AE24.
KOiK02100.
OMAiQIETDMA.
OrthoDBiEOG65J51S.
PhylomeDBiP0AE24.

Enzyme and pathway databases

BioCyciEcoCyc:ARAE-MONOMER.
ECOL316407:JW2809-MONOMER.
MetaCyc:ARAE-MONOMER.

Miscellaneous databases

PROiP0AE24.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian and bacterial sugar transport proteins are homologous."
    Maiden M.C.J., Davis E.O., Baldwin S.A., Moore D.C.M., Henderson P.J.F.
    Nature 325:641-643(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The cloning, DNA sequence, and overexpression of the gene araE coding for arabinose-proton symport in Escherichia coli K12."
    Maiden M.C.J., Jones-Mortimer M.C., Henderson P.J.F.
    J. Biol. Chem. 263:8003-8010(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / JM2433.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The araE low affinity L-arabinose transport promoter. Cloning, sequence, transcription start site and DNA binding sites of regulatory proteins."
    Stoner C., Schleif R.F.
    J. Mol. Biol. 171:369-381(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiARAE_ECOLI
AccessioniPrimary (citable) accession number: P0AE24
Secondary accession number(s): P09830, Q2M9Z6, Q46937
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.