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Protein

Class B acid phosphatase

Gene

aphA

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69NucleophileBy similarity1
Metal bindingi69MagnesiumBy similarity1
Active sitei71Proton donorBy similarity1
Metal bindingi71Magnesium; via carbonyl oxygenBy similarity1
Binding sitei177SubstrateBy similarity1
Metal bindingi192MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2By similarity)
Short name:
CBAP
Gene namesi
Name:aphA
Ordered Locus Names:SF4149, S3580
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Or 23Sequence analysisAdd BLAST25
ChainiPRO_000004337926 – 237Class B acid phosphataseAdd BLAST212

Proteomic databases

PaxDbiP0AE23.
PRIDEiP0AE23.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi198214.SF4149.

Structurei

3D structure databases

ProteinModelPortaliP0AE23.
SMRiP0AE23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 138Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AE23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKITQAISA VCLLFALNSS AVALASSPSP LNPGTNVARL AEQAPIHWVS
60 70 80 90 100
VAQIENSLAG RPPMAVGFDI DDTVLFSSPG FWRGKKTFSP ESEDYLKNPV
110 120 130 140 150
FWEKMNNGWD EFSIPKEVAR QLIDMHVRRG DAIFFVTGRS PTKTETVSKT
160 170 180 190 200
LADNFHIPAT NMNPVIFAGD KPGQNTKSQW LQDKNIRIFY GDSDNDITAA
210 220 230
RDVGARGIRI LRASNSTYKP LPQAGAFGEE VIVNSEY
Length:237
Mass (Da):26,104
Last modified:December 6, 2005 - v1
Checksum:i35C1BFE6DF9E530D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45571.2.
AE014073 Genomic DNA. Translation: AAP18626.1.
RefSeqiNP_709864.2. NC_004337.2.
WP_001226928.1. NZ_LVJC01000057.1.

Genome annotation databases

EnsemblBacteriaiAAN45571; AAN45571; SF4149.
AAP18626; AAP18626; S3580.
GeneIDi1027006.
KEGGisfl:SF4149.
sft:NCTC1_04512.
sfx:S3580.
PATRICi18708940. VBIShiFle31049_3908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45571.2.
AE014073 Genomic DNA. Translation: AAP18626.1.
RefSeqiNP_709864.2. NC_004337.2.
WP_001226928.1. NZ_LVJC01000057.1.

3D structure databases

ProteinModelPortaliP0AE23.
SMRiP0AE23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF4149.

Proteomic databases

PaxDbiP0AE23.
PRIDEiP0AE23.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN45571; AAN45571; SF4149.
AAP18626; AAP18626; S3580.
GeneIDi1027006.
KEGGisfl:SF4149.
sft:NCTC1_04512.
sfx:S3580.
PATRICi18708940. VBIShiFle31049_3908.

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_SHIFL
AccessioniPrimary (citable) accession number: P0AE23
Secondary accession number(s): P32697, P76787, Q57085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.