Protein AmpG - Escherichia coli (strain K12)

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P0AE16 (AMPG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein AmpG

Gene names

Name:	ampG
Ordered Locus Names:	b0433, JW0423

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably acts as a permease in the beta-lactamase induction system and in peptidoglycan recycling.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidoglycan metabolic process Inferred from genetic interaction PubMed 12426329. Source: EcoCyc peptidoglycan transport Inferred from genetic interaction PubMed 12426329. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	solute:hydrogen symporter activity Inferred from genetic interaction PubMed 12426329. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 491

491

Protein AmpG

PRO_0000084830

Regions

Topological domain

1 – 12

Cytoplasmic Potential

Transmembrane

13 – 33

Helical; Potential

Topological domain

34 – 80

Periplasmic Potential

Transmembrane

81 – 105

Helical; Potential

Topological domain

106 – 109

Cytoplasmic Potential

Transmembrane

110 – 128

Helical; Potential

Topological domain

129 – 173

Periplasmic Potential

Transmembrane

174 – 197

Helical; Potential

Topological domain

198 – 223

Cytoplasmic Potential

Transmembrane

224 – 244

Helical; Potential

Topological domain

245 – 265

Periplasmic Potential

Transmembrane

266 – 283

Helical; Potential

Topological domain

284 – 327

Cytoplasmic Potential

Transmembrane

328 – 345

Helical; Potential

Topological domain

346 – 381

Periplasmic Potential

Transmembrane

382 – 406

Helical; Potential

Topological domain

407 – 424

Cytoplasmic Potential

Transmembrane

425 – 450

Helical; Potential

Topological domain

451 – 460

Periplasmic Potential

Transmembrane

461 – 485

Helical; Potential

Topological domain

486 – 491

Cytoplasmic Potential

Natural variations

Natural variant

151

G → D in non-inducible mutant SN0301-1.

Natural variant

268

G → D in non-inducible mutant SN0301-3.

Natural variant

373

G → D in non-inducible mutant SN0301-5.

Sequences

Sequence

Length

Mass (Da)

Tools

P0AE16 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 6B0D7E2BC699CA9C
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FASTA

491

53,245

        10         20         30         40         50         60 
MSSQYLRIFQ QPRSAILLIL GFASGLPLAL TSGTLQAWMT VENIDLKTIG FFSLVGQAYV 

        70         80         90        100        110        120 
FKFLWSPLMD RYTPPFFGRR RGWLLATQIL LLVAIAAMGF LEPGTQLRWM AALAVVIAFC 

       130        140        150        160        170        180 
SASQDIVFDA WKTDVLPAEE RGAGAAISVL GYRLGMLVSG GLALWLADKW LGWQGMYWLM 

       190        200        210        220        230        240 
AALLIPCIIA TLLAPEPTDT IPVPKTLEQA VVAPLRDFFG RNNAWLILLL IVLYKLGDAF 

       250        260        270        280        290        300 
AMSLTTTFLI RGVGFDAGEV GVVNKTLGLL ATIVGALYGG ILMQRLSLFR ALLIFGILQG 

       310        320        330        340        350        360 
ASNAGYWLLS ITDKHLYSMG AAVFFENLCG GMGTSAFVAL LMTLCNKSFS ATQFALLSAL 

       370        380        390        400        410        420 
SAVGRVYVGP VAGWFVEAHG WSTFYLFSVA AAVPGLILLL VCRQTLEYTR VNDNFISRTA 

       430        440        450        460        470        480 
YPAGYAFAMW TLAAGVSLLA VWLLLLTMDA LDLTHFSFLP ALLEVGVLVA LSGVVLGGLL 

       490 
DYLALRKTHL T

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AmpG, a signal transducer in chromosomal beta-lactamase induction." Lindquist S., Weston-Hafer K., Schmidt H., Pul C., Korfmann G., Erickson J., Sanders C., Martin H.H., Normark S. Mol. Microbiol. 9:703-715(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The signal transducer encoded by ampG is essential for induction of chromosomal AmpC beta-lactamase in Escherichia coli by beta-lactam antibiotics and 'unspecific' inducers." Schmidt H., Korfmann G., Barth H., Martin H.H. Microbiology 141:1085-1092(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SN0301-1, SN0301-3 and SN0301-5.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S67816 Genomic DNA. Translation: AAB28884.1. X82158 mRNA. Translation: CAA57651.1. X82159 mRNA. Translation: CAA57652.1. X82160 mRNA. Translation: CAA57653.1. U82664 Genomic DNA. Translation: AAB40189.1. U00096 Genomic DNA. Translation: AAC73536.1. AP009048 Genomic DNA. Translation: BAE76213.1.
PIR	S37391.
RefSeq	NP_414967.1. NC_000913.2. YP_488725.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AE16.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0433.
Protein family/group databases
TCDB	2.A.1.25.2. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73536; AAC73536; b0433. BAE76213; BAE76213; BAE76213.
GeneID	12930844. 946438.
KEGG	ecj:Y75_p0421. eco:b0433.
PATRIC	32116017. VBIEscCol129921_0450.
Organism-specific databases
EchoBASE	EB2100.
EcoGene	EG12183. ampG.
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000217208.
KO	K08218.
OMA	AKNAGLW.
ProtClustDB	PRK11010.
Enzyme and pathway databases
BioCyc	EcoCyc:AMPG-MONOMER. ECOL316407:JW0423-MONOMER.
Gene expression databases
Genevestigator	P0AE16.
Family and domain databases
InterPro	IPR004752. AmpG_permease/AT-1. IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00901. 2A0125. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPG_ECOLI

Accession

Primary (citable) accession number: P0AE16
Secondary accession number(s): P36670, Q2MBZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents