P0AE13 (AMN_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AMP nucleosidase
EC=3.2.2.4

Gene names

Name:	amn
Ordered Locus Names:	Z3139, ECs2779

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in regulation of AMP concentrations By similarity.
Catalytic activity	AMP + H2O = D-ribose 5-phosphate + adenine.
Enzyme regulation	Allosterically activated by Mg-ATP, and inactivated by inorganic phosphate By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
Miscellaneous	AMP nucleosidase binds AMP at the catalytic site, Mg-ATP at an allosteric regulatory site, and inorganic phosphate also at a regulatory site By similarity.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	AMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	AMP nucleosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	AMP nucleosidase		PRO_0000064587

Sequences

	Sequence		Length	Mass (Da)
	P0AE13 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: ADC2FD95CC220527	FASTA	484	53,995
	10 20 30 40 50 60 MNNKGSGLTP AQALDKLDAL YEQSVVALRN AIGNYITSGE LPDENARKQG LFVYPSLTVT 70 80 90 100 110 120 WDGSTTNPPK TRAFGRFTHA GSYTTTITRP TLFRSYLNEQ LTLLYQDYGA HISVQPSQHE 130 140 150 160 170 180 IPYPYVIDGS ELTLDRSMSA GLTRYFPTTE LAQIGDETAD GIYHPTEFSP LSHFDARRVD 190 200 210 220 230 240 FSLARLRHYT GTPVEHFQPF VLFTNYTRYV DEFVRWGCSQ ILDPDSPYIA LSCAGGNWIT 250 260 270 280 290 300 AETEAPEEAI SDLAWKKHQM PAWHLITADG QGITLVNIGV GPSNAKTICD HLAVLRPDVW 310 320 330 340 350 360 LMIGHCGGLR ESQAIGDYVL AHAYLRDDHV LDAVLPPDIP IPSIAEVQRA LYDATKLVSG 370 380 390 400 410 420 RPGEEVKQRL RTGTVVTTDD RNWELRYSAS ALRFNLSRAV AIDMESATIA AQGYRFRVPY 430 440 450 460 470 480 GTLLCVSDKP LHGEIKLPGQ ANRFYEGAIS EHLQIGIRAI DLLRAEGDRL HSRKLRTFNE PPFR

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57045.1. BA000007 Genomic DNA. Translation: BAB36202.1.
PIR	A85823. C90976.
RefSeq	NP_288491.1. NC_002655.2. NP_310806.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AE13.
SMR	P0AE13. Positions 8-484.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z3139.
Proteomic databases
PRIDE	P0AE13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG57045; AAG57045; Z3139. BAB36202; BAB36202; BAB36202.
GeneID	912716. 962041.
KEGG	ece:Z3139. ecs:ECs2779.
PATRIC	18354950. VBIEscCol44059_2679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0775.
HOGENOM	HOG000156781.
KO	K01241.
OMA	TPIPLHF.
ProtClustDB	PRK08292.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-2743-MONOMER.
UniPathway	UPA00588; UER00646.
Family and domain databases
Gene3D	3.30.1730.10. 1 hit.
InterPro	IPR011271. AMP_nucleosidase. IPR018953. AMP_nucleoside_Pase_N. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
PANTHER	PTHR21234. PTHR21234. 1 hit.
Pfam	PF10423. AMNp_N. 1 hit. PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01717. AMP-nucleosdse. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMN_ECO57

Accession

Primary (citable) accession number: P0AE13
Secondary accession number(s): P15272, P78074

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program