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P0AE13 (AMN_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AMP nucleosidase

EC=3.2.2.4
Gene names
Name:amn
Ordered Locus Names:Z3139, ECs2779
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in regulation of AMP concentrations By similarity.

Catalytic activity

AMP + H2O = D-ribose 5-phosphate + adenine.

Enzyme regulation

Allosterically activated by Mg-ATP, and inactivated by inorganic phosphate By similarity.

Pathway

Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.

Miscellaneous

AMP nucleosidase binds AMP at the catalytic site, Mg-ATP at an allosteric regulatory site, and inorganic phosphate also at a regulatory site By similarity.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termAllosteric enzyme
Complete proteome
Gene Ontology (GO)
   Biological_processAMP salvage

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionAMP nucleosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484AMP nucleosidase
PRO_0000064587

Sequences

Sequence LengthMass (Da)Tools
P0AE13 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: ADC2FD95CC220527

FASTA48453,995
        10         20         30         40         50         60 
MNNKGSGLTP AQALDKLDAL YEQSVVALRN AIGNYITSGE LPDENARKQG LFVYPSLTVT 

        70         80         90        100        110        120 
WDGSTTNPPK TRAFGRFTHA GSYTTTITRP TLFRSYLNEQ LTLLYQDYGA HISVQPSQHE 

       130        140        150        160        170        180 
IPYPYVIDGS ELTLDRSMSA GLTRYFPTTE LAQIGDETAD GIYHPTEFSP LSHFDARRVD 

       190        200        210        220        230        240 
FSLARLRHYT GTPVEHFQPF VLFTNYTRYV DEFVRWGCSQ ILDPDSPYIA LSCAGGNWIT 

       250        260        270        280        290        300 
AETEAPEEAI SDLAWKKHQM PAWHLITADG QGITLVNIGV GPSNAKTICD HLAVLRPDVW 

       310        320        330        340        350        360 
LMIGHCGGLR ESQAIGDYVL AHAYLRDDHV LDAVLPPDIP IPSIAEVQRA LYDATKLVSG 

       370        380        390        400        410        420 
RPGEEVKQRL RTGTVVTTDD RNWELRYSAS ALRFNLSRAV AIDMESATIA AQGYRFRVPY 

       430        440        450        460        470        480 
GTLLCVSDKP LHGEIKLPGQ ANRFYEGAIS EHLQIGIRAI DLLRAEGDRL HSRKLRTFNE 


PPFR 

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG57045.1.
BA000007 Genomic DNA. Translation: BAB36202.1.
PIRA85823.
C90976.
RefSeqNP_288491.1. NC_002655.2.
NP_310806.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0AE13.
SMRP0AE13. Positions 8-484.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z3139.

Proteomic databases

PRIDEP0AE13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG57045; AAG57045; Z3139.
BAB36202; BAB36202; BAB36202.
GeneID912716.
962041.
KEGGece:Z3139.
ecs:ECs2779.
PATRIC18354950. VBIEscCol44059_2679.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0775.
HOGENOMHOG000156781.
KOK01241.
OMAQNIPYPY.
OrthoDBEOG67DPGS.

Enzyme and pathway databases

BioCycECOL386585:GJFA-2743-MONOMER.
ECOO157:AMN-MONOMER.
UniPathwayUPA00588; UER00646.

Family and domain databases

Gene3D3.30.1730.10. 1 hit.
3.40.50.1580. 1 hit.
InterProIPR011271. AMP_nucleosidase.
IPR018953. AMP_nucleoside_Pase_N.
IPR018017. Nucleoside_phosphorylase.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERPTHR21234. PTHR21234. 1 hit.
PfamPF10423. AMNp_N. 1 hit.
PF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01717. AMP-nucleosdse. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMN_ECO57
AccessionPrimary (citable) accession number: P0AE13
Secondary accession number(s): P15272, P78074
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways