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Reviewed, UniProtKB/Swiss-Prot P0AE06 (ACRA_ECOLI)

Last modified November 24, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Acriflavine resistance protein A
Gene names
Name: acrA
Synonyms: lir, mtcA
Ordered Locus Names: b0463, JW0452
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

AcrAB is a drug efflux protein with a broad substrate specificity.

Subunit structure

Homotrimeric; interacts with acrB. Ref.7 Ref.8

Subcellular location

Cell inner membrane; Lipid-anchor.

Sequence similarities

Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. [View classification]

Binary interactions

With

Entry

#Exp.

IntAct

Notes

acrBP312241EBI-875601,EBI-551006

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 397373Acriflavine resistance protein A
PRO_0000018687

Amino acid modifications

Lipidation251N-palmitoyl cysteine Potential
Lipidation251S-diacylglycerol cysteine Potential

Secondary structure

................................. 397
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AE06-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 5B81DD5BC2B0A077

FASTA39742,197
        10         20         30         40         50         60 
MNKNRGFTPL AVVLMLSGSL ALTGCDDKQA QQGGQQMPAV GVVTVKTEPL QITTELPGRT 

        70         80         90        100        110        120 
SAYRIAEVRP QVSGIILKRN FKEGSDIEAG VSLYQIDPAT YQATYDSAKG DLAKAQAAAN 

       130        140        150        160        170        180 
IAQLTVNRYQ KLLGTQYISK QEYDQALADA QQANAAVTAA KAAVETARIN LAYTKVTSPI 

       190        200        210        220        230        240 
SGRIGKSNVT EGALVQNGQA TALATVQQLD PIYVDVTQSS NDFLRLKQEL ANGTLKQENG 

       250        260        270        280        290        300 
KAKVSLITSD GIKFPQDGTL EFSDVTVDQT TGSITLRAIF PNPDHTLLPG MFVRARLEEG 

       310        320        330        340        350        360 
LNPNAILVPQ QGVTRTPRGD ATVLVVGADD KVETRPIVAS QAIGDKWLVT EGLKAGDRVV 

       370        380        390 
ISGLQKVRPG VQVKAQEVTA DNNQQAASGA QPEQSKS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of acrA and acrE genes of Escherichia coli."
Ma D., Cook D.N., Alberti M., Pon N.G., Nikaido H., Hearst J.E.
J. Bacteriol. 175:6299-6313(1993) [PubMed: 8407802] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W4573.
[2]"Nucleotide sequence of the acrAB operon from Escherichia coli."
Xu J., Bertrand K.P.
Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli."
Ma D., Cook D.N., Alberti M., Pon N.G., Nikaido H., Hearst J.E.
Mol. Microbiol. 16:45-55(1995) [PubMed: 7651136] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Molecular construction of a multidrug exporter system, AcrAB: molecular interaction between AcrA and AcrB, and cleavage of the N-terminal signal sequence of AcrA."
Kawabe T., Fujihira E., Yamaguchi A.
J. Biochem. 128:195-200(2000) [PubMed: 10920254] [Abstract]
Cited for: PROTEOLYTIC PROCESSING, INTERACTION WITH ACRB.
[8]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract]
Cited for: HOMOTRIMERIZATION, SUBCELLULAR LOCATION.
Strain: BL21-DE3.
+Additional computationally mapped references.

Cross-references

Sequence databases

U00734 Genomic DNA. Translation: AAA67134.1.
M94248 Genomic DNA. Translation: AAA23410.1.
U82664 Genomic DNA. Translation: AAB40217.1.
U00096 Genomic DNA. Translation: AAC73565.1.
AP009048 Genomic DNA. Translation: BAE76242.1.
PIRA36938.
RefSeqAP_001112.1.
NP_414996.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2F1MX-ray2.71A/B/C/D45-312[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:29039N.
IntActP0AE06. 3 interactions.
STRINGP0AE06.

Protein family/group databases

TCDB8.A.1.6.1. membrane fusion protein (MFP) family.

Proteomic databases

PRIDEP0AE06.

Genome annotation databases

GeneID945112.
GenomeReviewsGene locus JW0452 in contig AP009048_GR.
Gene locus b0463 in contig U00096_GR.
KEGGecj:JW0452.
eco:b0463.

Organism-specific databases

EchoBASEEB1654.
EcoGeneEG11703. acrA.
CMRSearch...

Phylogenomic databases

HOGENOMP0AE06.
OMATEGLQYV

Enzyme and pathway databases

BioCycEcoCyc:EG11703-MON.
ECOL168927:B0463-MON.

Gene expression databases

GenevestigatorP0AE06.

Family and domain databases

InterProIPR006143. Secretion_HlyD.
[Graphical view]
PfamPF00529. HlyD. 1 hit.
[Graphical view]
TIGRFAMsTIGR01730. RND_mfp. 1 hit.
PROSITEPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACRA_ECOLI
AccessionPrimary (citable) accession number: P0AE06
Secondary accession number(s): P31223, Q2MBW4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents