P0AE05 (AADB1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2''-aminoglycoside nucleotidyltransferase
EC=2.7.7.46

Alternative name(s):
AAD(2'')
Gentamicin 2''-nucleotidyltransferase
Gentamicin resistance protein

Gene names

Name:

aadB

Encoded on

Plasmid pBP201 Ref.1
Plasmid IncM pBWH1 Ref.2

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	This enzyme confers resistance to kanamycin, gentamicin, dibekacin, sisomicin and tobramycin by adenylating the 2''-hydroxyl group of these antibiotics.
Catalytic activity	Nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Nucleotidyltransferase Transferase
Technical term	Plasmid Transposable element
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminoglycoside 2''-nucleotidyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	2''-aminoglycoside nucleotidyltransferase		PRO_0000068557

Sequences

	Sequence		Length	Mass (Da)
	P0AE05 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 9A084D96AE99F2F1	FASTA	177	19,873
	10 20 30 40 50 60 MDTTQVTLIH KILAAADERN LPLWIGGGWA IDARLGRVTR KHDDIDLTFP GERRGELEAI 70 80 90 100 110 120 VEMLGGRVME ELDYGFLAEI GDELLDCEPA WWADEAYEIA EAPQGSCPEA AEGVIAGRPV 130 140 150 160 170 RCNSWEAIIW DYFYYADEVP PVDWPTKHIE SYRLACTSLG AEKVEVLRAA FRSRYAA

References

[1]	"Nucleotide sequence analysis of 2''-aminoglycoside nucleotidyl-transferase ANT(2'') from Tn4000: its relationship with AAD(3'') and impact on Tn21 evolution." Schmidt F.R.J., Nuecken E.J., Henschke R.B. Mol. Microbiol. 2:709-717(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 18440. Transposon: Tn4000.
[2]	"Molecular characterization and comparison of the regions encoding gentamicin resistance (aadB) in pBW1 and pLST1000 and their relationship to Tn21 family of transposons and elements." Zieg J., Jiang H., McCabe F., O'Brien T. Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12618 Genomic DNA. Translation: CAA31139.1. X64368 Genomic DNA. Translation: CAA45721.1.
PIR	XNKBGP. S04170.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019646. Aminoglyc_AdlTrfase. [Graphical view]
Pfam	PF10706. Aminoglyc_resit. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AADB1_KLEPN

Accession

Primary (citable) accession number: P0AE05
Secondary accession number(s): P08880, P10019

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program