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Protein

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

Gene

trmJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA.
S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(32) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei134S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei143S-adenosyl-L-methionine; via amide nitrogenBy similarity1

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • tRNA (cytosine-2'-O-)-methyltransferase activity Source: EcoCyc
  • tRNA (uracil-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA nucleoside ribose methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7327-MONOMER.
ECOL316407:JW2516-MONOMER.
MetaCyc:G7327-MONOMER.
BRENDAi2.1.1.200. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ (EC:2.1.1.200)
Alternative name(s):
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase
tRNA Cm32/Um32 methyltransferase
Gene namesi
Name:trmJ
Synonyms:yfhQ
Ordered Locus Names:b2532, JW2516
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13452. trmJ.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598241 – 246tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJAdd BLAST246

Proteomic databases

PaxDbiP0AE01.
PRIDEiP0AE01.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4263050. 2 interactors.
IntActiP0AE01. 3 interactors.
STRINGi511145.b2532.

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Beta strandi5 – 11Combined sources7
Helixi15 – 27Combined sources13
Beta strandi32 – 37Combined sources6
Helixi44 – 50Combined sources7
Helixi51 – 53Combined sources3
Helixi54 – 58Combined sources5
Beta strandi61 – 64Combined sources4
Helixi66 – 70Combined sources5
Beta strandi74 – 79Combined sources6
Beta strandi84 – 86Combined sources3
Helixi93 – 104Combined sources12
Beta strandi109 – 113Combined sources5
Turni116 – 118Combined sources3
Helixi122 – 125Combined sources4
Beta strandi129 – 132Combined sources4
Helixi145 – 162Combined sources18
Helixi180 – 196Combined sources17
Helixi207 – 218Combined sources12
Helixi223 – 237Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CNDX-ray1.50A/B1-246[»]
4CNEX-ray1.90A/B1-246[»]
4XBOX-ray2.60A/B1-246[»]
ProteinModelPortaliP0AE01.
SMRiP0AE01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CVS. Bacteria.
COG0565. LUCA.
HOGENOMiHOG000229628.
InParanoidiP0AE01.
KOiK15396.
OMAiELQRCHF.
PhylomeDBiP0AE01.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR004384. rRNA_MeTrfase_TrmH_1.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004808. LasT. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00050. rRNA_methyl_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AE01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQNIRIVLV ETSHTGNMGS VARAMKTMGL TNLWLVNPLV KPDSQAIALA
60 70 80 90 100
AGASDVIGNA HIVDTLDEAL AGCSLVVGTS ARSRTLPWPM LDPRECGLKS
110 120 130 140 150
VAEAANTPVA LVFGRERVGL TNEELQKCHY HVAIAANPEY SSLNLAMAVQ
160 170 180 190 200
VIAYEVRMAW LATQENGEQV EHEETPYPLV DDLERFYGHL EQTLLATGFI
210 220 230 240
RENHPGQVMN KLRRLFTRAR PESQELNILR GILASIEQQN KGNKAE
Length:246
Mass (Da):27,048
Last modified:December 6, 2005 - v1
Checksum:i83C1AE4507E097D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75585.1.
AP009048 Genomic DNA. Translation: BAA16426.1.
PIRiC65030.
RefSeqiNP_417027.1. NC_000913.3.
WP_000940019.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75585; AAC75585; b2532.
BAA16426; BAA16426; BAA16426.
GeneIDi948610.
KEGGiecj:JW2516.
eco:b2532.
PATRICi32120461. VBIEscCol129921_2633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75585.1.
AP009048 Genomic DNA. Translation: BAA16426.1.
PIRiC65030.
RefSeqiNP_417027.1. NC_000913.3.
WP_000940019.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CNDX-ray1.50A/B1-246[»]
4CNEX-ray1.90A/B1-246[»]
4XBOX-ray2.60A/B1-246[»]
ProteinModelPortaliP0AE01.
SMRiP0AE01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263050. 2 interactors.
IntActiP0AE01. 3 interactors.
STRINGi511145.b2532.

Proteomic databases

PaxDbiP0AE01.
PRIDEiP0AE01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75585; AAC75585; b2532.
BAA16426; BAA16426; BAA16426.
GeneIDi948610.
KEGGiecj:JW2516.
eco:b2532.
PATRICi32120461. VBIEscCol129921_2633.

Organism-specific databases

EchoBASEiEB3225.
EcoGeneiEG13452. trmJ.

Phylogenomic databases

eggNOGiENOG4105CVS. Bacteria.
COG0565. LUCA.
HOGENOMiHOG000229628.
InParanoidiP0AE01.
KOiK15396.
OMAiELQRCHF.
PhylomeDBiP0AE01.

Enzyme and pathway databases

BioCyciEcoCyc:G7327-MONOMER.
ECOL316407:JW2516-MONOMER.
MetaCyc:G7327-MONOMER.
BRENDAi2.1.1.200. 2026.

Miscellaneous databases

PROiP0AE01.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR004384. rRNA_MeTrfase_TrmH_1.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004808. LasT. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00050. rRNA_methyl_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMJ_ECOLI
AccessioniPrimary (citable) accession number: P0AE01
Secondary accession number(s): P76993, P77438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.