Reviewed, UniProtKB/Swiss-Prot P0ADZ4 (RS15_ECOLI)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S15

Gene names

Name:	rpsO
Synonyms:	secC
Ordered Locus Names:	b3165, JW3134

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. HAMAP MF_01343 In the E.coli 70S ribosome it has been modeled (Ref.11) to contact the 23S rRNA of the 50S subunit forming part of bridge B4. In the two 3.5 A resolved ribosome structures (Ref.12) there are minor differences between side-chain conformations. HAMAP MF_01343
Subunit structure	Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA. Ref.11 Ref.12
Sequence similarities	Belongs to the ribosomal protein S15P family.
Mass spectrometry	Molecular mass is 10137.6 Da from positions 2 - 89. Determined by MALDI. Ref.9

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.7

Chain

2 – 89

88

30S ribosomal protein S15 HAMAP MF_01343

PRO_0000115437

Experimental info

Sequence conflict

66

1

Missing Ref.3

Secondary structure

1

.

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.

.

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89

Helix Strand Turn

Details...

Helix

5 – 12

8

Turn

13 – 15

3

Beta strand

17 – 20

4

Helix

25 – 44

20

Helix

50 – 73

24

Helix

75 – 82

8

Turn

83 – 85

3

Sequences

	Sequence		Length	Mass (Da)
	P0ADZ4-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AABDDA6674B99B1C	FASTA	89	10,269
	10 20 30 40 50 60 MSLSTEATAK IVSEFGRDAN DTGSTEVQVA LLTAQINHLQ GHFAEHKKDH HSRRGLLRMV 70 80 SQRRKLLDYL KRKDVARYTQ LIERLGLRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO)." Takata R., Mukai T., Aoyagi M., Hori K. Mol. Gen. Genet. 197:225-229(1984) [PubMed: 6394953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Expression of the rpsO and pnp genes: structural analysis of a DNA fragment carrying their control regions." Portier C., Regnier P. Nucleic Acids Res. 12:6091-6102(1984) [PubMed: 6382163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Promoter activity and transcript mapping in the regulatory region for genes encoding ribosomal protein S15 and polynucleotide phosphorylase of Escherichia coli." Evans S., Dennis P.P. Gene 40:15-22(1985) [PubMed: 3005122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein S1." Regnier P., Grunberg-Manago M., Portier C. J. Biol. Chem. 262:63-68(1987) [PubMed: 2432069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Primary structure of the 16S rRNA binding protein S15 from Escherichia coli ribosomes." Morinaga T., Funatsu G., Funatsu M., Wittmann H.G. FEBS Lett. 64:307-309(1976) [PubMed: 776686] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-89. Strain: K.
[8]	"The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping." Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B. Nucleic Acids Res. 16:10803-10816(1988) [PubMed: 2849753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[9]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[10]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[11]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS), INTERSUBUNIT BRIDGE FORMATION. Strain: MRE-600.
[12]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES INCLUDING THE INTERSUBUNIT BRIDGE. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01073 Genomic DNA. Translation: CAA25536.1.
X00761 Genomic DNA. Translation: CAA25331.1.
M14425 Genomic DNA. Translation: AAA24595.1.
J02638 Genomic DNA. Translation: AAA83904.1.
U18997 Genomic DNA. Translation: AAA57968.1.
U00096 Genomic DNA. Translation: AAC76199.1.
AP009048 Genomic DNA. Translation: BAE77211.1.
X13775 Genomic DNA. Translation: CAA32022.1.

PIR

R3EC15. B26118.

RefSeq

AP_003710.1.
NP_417634.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P6G	electron microscopy	12.30	O	1-89	[»]
1P87	electron microscopy	11.50	O	1-89	[»]
2AVY	X-ray	3.46	O	1-89	[»]
2AW7	X-ray	3.46	O	1-89	[»]
2GY9	electron microscopy	-	O	3-87	[»]
2GYB	electron microscopy	-	O	3-87	[»]
2QAL	X-ray	3.21	O	1-89	[»]
2QAN	X-ray	3.21	O	1-89	[»]
2QB9	X-ray	3.54	O	1-89	[»]
2QBB	X-ray	3.54	O	1-89	[»]
2QBD	X-ray	3.30	O	1-89	[»]
2QBF	X-ray	3.30	O	1-89	[»]
2QBH	X-ray	4.00	O	1-89	[»]
2QBJ	X-ray	4.00	O	1-89	[»]
2QOU	X-ray	3.93	O	1-89	[»]
2QOW	X-ray	3.93	O	1-89	[»]
2QOY	X-ray	3.50	O	1-89	[»]
2QP0	X-ray	3.50	O	1-89	[»]
2VAZ	electron microscopy	10.00	F	2-88	[»]
2Z4K	X-ray	4.45	O	1-89	[»]
2Z4M	X-ray	4.45	O	1-89	[»]
3FIH	electron microscopy	-	O	2-89	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

I012.0. 6TH EDITION.

Genome annotation databases

GeneID

947686.

GenomeReviews

Gene locus JW3134 in contig AP009048_GR.
Gene locus b3165 in contig U00096_GR.

KEGG

ecj:JW3134.
eco:b3165.

Organism-specific databases

EchoBASE

EB0907.

EcoGene

EG10914. rpsO.

CMR

Search...

Phylogenomic databases

HOGENOM

P0ADZ4.

OMA

P0ADZ4. YLRNKDV.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10914-MON.

Family and domain databases

HAMAP

MF_01343.
[Tree]

InterPro

IPR000589. Ribosomal_S15.
IPR005290. Ribosomal_S15_bac-type.
IPR009068. S15_NS1_RNA_bd.
[Graphical view]

Gene3D

G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.

PANTHER

PTHR23321:SF10. Ribosom_S15_bac. 1 hit.

Pfam

PF00312. Ribosomal_S15. 1 hit.
[Graphical view]

ProDom

PD157043. Ribosomal_S15_b. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00952. S15_bact. 1 hit.

PROSITE

PS00362. RIBOSOMAL_S15. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RS15_ECOLI

Accession

Primary (citable) accession number: P0ADZ4
Secondary accession number(s): P02371, Q2M945

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)