P0ADY5 (RL14_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L14

Gene names

Name:	rplN
Ordered Locus Names:	Z4680, ECs4175

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome By similarity. HAMAP MF_01367_B
Subunit structure	Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8 By similarity.
Sequence similarities	Belongs to the ribosomal protein L14P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 123

123

50S ribosomal protein L14 HAMAP MF_01367_B

PRO_0000128541

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

123

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Beta strand

19 – 21

3

Beta strand

39 – 45

7

Beta strand

57 – 60

4

Helix

89 – 91

3

Turn

110 – 112

3

Helix

115 – 117

3

Beta strand

118 – 120

3

Sequences

	Sequence		Length	Mass (Da)
	P0ADY5 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 192ACB1623979510	FASTA	123	13,541
	10 20 30 40 50 60 MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA 70 80 90 100 110 120 VVVRTKKGVR RPDGSVIRFD GNACVLLNNN SEQPIGTRIF GPVTRELRSE KFMKIISLAP EVL

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE005174 Genomic DNA. Translation: AAG58431.1.
BA000007 Genomic DNA. Translation: BAB37598.1.

PIR

C85996.
G91150.

RefSeq

NP_289871.1. NC_002655.2.
NP_312202.1. NC_002695.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DF2	X-ray	3.50	K	1-123	[»]
3DF4	X-ray	3.50	K	1-123	[»]

ProteinModelPortal

P0ADY5.

SMR

P0ADY5. Positions 2-123.

ModBase

Search...

Proteomic databases

PRIDE

P0ADY5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000024763; EBESCP00000023656; EBESCG00000023817.
EBESCT00000057238; EBESCP00000055066; EBESCG00000056286.

GeneID

915973.
958775.

GenomeReviews

Gene locus Z4680 in contig AE005174_GR.
Gene locus ECs4175 in contig BA000007_GR.

KEGG

ece:Z4680.
ecs:ECs4175.

PATRIC

18357898. VBIEscCol44059_4116.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000009221.

HOGENOM

HBG594899.

OMA

MIQKETN.

ProtClustDB

PRK05483.

Enzyme and pathway databases

BioCyc

ECOL83334:ECS4175-MONOMER.

Family and domain databases

HAMAP

MF_01367_B. Ribosomal_L14_B.
[Tree]

InterPro

IPR005745. Ribosomal_L14_bac-type.
IPR019972. Ribosomal_L14_CS.
IPR023571. Ribosomal_L14_dom.
IPR000218. Ribosomal_L14b/L23e.
[Graphical view]

Gene3D

G3DSA:2.40.150.20. Ribosomal_L14. 1 hit.

KO

K02874.

PANTHER

PTHR11761. Ribosomal_L14. 1 hit.

Pfam

PF00238. Ribosomal_L14. 1 hit.
[Graphical view]

SUPFAM

SSF50193. Ribosomal_L14. 1 hit.

TIGRFAMs

TIGR01067. RplN_bact. 1 hit.

PROSITE

PS00049. RIBOSOMAL_L14. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL14_ECO57

Accession

Primary (citable) accession number: P0ADY5
Secondary accession number(s): P02411

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 1, 1988
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program