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P0ADY1

- PPID_ECOLI

UniProt

P0ADY1 - PPID_ECOLI

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Protein
Peptidyl-prolyl cis-trans isomerase D
Gene
ppiD, ybaU, b0441, JW0431
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. Seems to be involved in the folding of outer membrane proteins. Its preference at the P1 position of the peptide substrate is Glu > Leu > Ala > His > Val > Phe > Ile > Gly > Lys > Thr.

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB-KW

GO - Biological processi

  1. protein folding Source: UniProtKB-KW
  2. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:G6242-MONOMER.
ECOL316407:JW0431-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase D (EC:5.2.1.8)
Short name:
PPIase D
Alternative name(s):
Rotamase D
Gene namesi
Name:ppiD
Synonyms:ybaU
Ordered Locus Names:b0441, JW0431
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13249. ppiD.

Subcellular locationi

Cell inner membrane; Single-pass type II membrane protein; Periplasmic side 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei16 – 3621Helical; Reviewed prediction
Add
BLAST
Topological domaini37 – 623587Periplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi312 – 3121G → A or R: Loss of activity.
Mutagenesisi313 – 3131G → A or R: Loss of activity.
Mutagenesisi347 – 3471G → A: Loss of activity.
Mutagenesisi350 – 3501I → A or F: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Peptidyl-prolyl cis-trans isomerase D
PRO_0000193423Add
BLAST

Proteomic databases

PaxDbiP0ADY1.
PRIDEiP0ADY1.

Expressioni

Inductioni

By heat shock.

Gene expression databases

GenevestigatoriP0ADY1.

Interactioni

Subunit structurei

Has been isolated as a homodimer and homotrimer from inner membrane preparations.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-562001,EBI-562001

Protein-protein interaction databases

DIPiDIP-39902N.
IntActiP0ADY1. 8 interactions.
STRINGi511145.b0441.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi268 – 27811
Helixi279 – 29113
Helixi295 – 3017
Helixi306 – 3094
Turni310 – 3123
Beta strandi313 – 3197
Helixi325 – 3284
Beta strandi338 – 3447
Beta strandi347 – 35711

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KGJNMR-A264-357[»]
ProteinModelPortaliP0ADY1.
SMRiP0ADY1. Positions 86-422.

Miscellaneous databases

EvolutionaryTraceiP0ADY1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini266 – 35590PpiC
Add
BLAST

Sequence similaritiesi

Contains 1 PpiC domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0760.
HOGENOMiHOG000276975.
KOiK03770.
OMAiQNIRDNS.
OrthoDBiEOG6M9DS4.
PhylomeDBiP0ADY1.

Family and domain databases

InterProiIPR000297. PPIase_PpiC.
IPR023058. PPIase_PpiC_CS.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]
PfamiPF13145. Rotamase_2. 1 hit.
[Graphical view]
SUPFAMiSSF109998. SSF109998. 2 hits.
PROSITEiPS01096. PPIC_PPIASE_1. 1 hit.
PS50198. PPIC_PPIASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ADY1-1 [UniParc]FASTAAdd to Basket

« Hide

MMDSLRTAAN SLVLKIIFGI IIVSFILTGV SGYLIGGGNN YAAKVNDQEI    50
SRGQFENAFN SERNRMQQQL GDQYSELAAN EGYMKTLRQQ VLNRLIDEAL 100
LDQYARELKL GISDEQVKQA IFATPAFQVD GKFDNSRYNG ILNQMGMTAD 150
QYAQALRNQL TTQQLINGVA GTDFMLKGET DELAALVAQQ RVVREATIDV 200
NALAAKQPVT EQEIASYYEQ NKNNFMTPEQ FRVSYIKLDA ATMQQPVSDA 250
DIQSYYDQHQ DQFTQPQRTR YSIIQTKTED EAKAVLDELN KGGDFAALAK 300
EKSADIISAR NGGDMGWLED ATIPDELKNA GLKEKGQLSG VIKSSVGFLI 350
VRLDDIQPAK VKSLDEVRDD IAAKVKHEKA LDAYYALQQK VSDAASNDTE 400
SLAGAEQAAG VKATQTGWFS KDNLPEELNF KPVADAIFNG GLVGENGAPG 450
INSDIITVDG DRAFVLRISE HKPEAVKPLA DVQEQVKALV QHNKAEQQAK 500
VDAEKLLVDL KAGKGAEAMQ AAGLKFGEPK TLSRSGRDPI SQAAFALPLP 550
AKDKPSYGMA TDMQGNVVLL ALDEVKQGSM PEDQKKAMVQ GITQNNAQIV 600
FEALMSNLRK EAKIKIGDAL EQQ 623
Length:623
Mass (Da):68,150
Last modified:December 6, 2005 - v1
Checksum:i0F646F687114A387
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82943 Genomic DNA. Translation: BAA11645.1.
U82664 Genomic DNA. Translation: AAB40197.1.
U00096 Genomic DNA. Translation: AAC73544.1.
AP009048 Genomic DNA. Translation: BAE76221.1.
PIRiA64774.
RefSeqiNP_414975.1. NC_000913.3.
YP_488733.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73544; AAC73544; b0441.
BAE76221; BAE76221; BAE76221.
GeneIDi12931738.
946056.
KEGGiecj:Y75_p0429.
eco:b0441.
PATRICi32116035. VBIEscCol129921_0459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82943 Genomic DNA. Translation: BAA11645.1 .
U82664 Genomic DNA. Translation: AAB40197.1 .
U00096 Genomic DNA. Translation: AAC73544.1 .
AP009048 Genomic DNA. Translation: BAE76221.1 .
PIRi A64774.
RefSeqi NP_414975.1. NC_000913.3.
YP_488733.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KGJ NMR - A 264-357 [» ]
ProteinModelPortali P0ADY1.
SMRi P0ADY1. Positions 86-422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-39902N.
IntActi P0ADY1. 8 interactions.
STRINGi 511145.b0441.

Proteomic databases

PaxDbi P0ADY1.
PRIDEi P0ADY1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73544 ; AAC73544 ; b0441 .
BAE76221 ; BAE76221 ; BAE76221 .
GeneIDi 12931738.
946056.
KEGGi ecj:Y75_p0429.
eco:b0441.
PATRICi 32116035. VBIEscCol129921_0459.

Organism-specific databases

EchoBASEi EB3038.
EcoGenei EG13249. ppiD.

Phylogenomic databases

eggNOGi COG0760.
HOGENOMi HOG000276975.
KOi K03770.
OMAi QNIRDNS.
OrthoDBi EOG6M9DS4.
PhylomeDBi P0ADY1.

Enzyme and pathway databases

BioCyci EcoCyc:G6242-MONOMER.
ECOL316407:JW0431-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ADY1.
PROi P0ADY1.

Gene expression databases

Genevestigatori P0ADY1.

Family and domain databases

InterProi IPR000297. PPIase_PpiC.
IPR023058. PPIase_PpiC_CS.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view ]
Pfami PF13145. Rotamase_2. 1 hit.
[Graphical view ]
SUPFAMi SSF109998. SSF109998. 2 hits.
PROSITEi PS01096. PPIC_PPIASE_1. 1 hit.
PS50198. PPIC_PPIASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Hatada E., Ohmori H., Qiao Y., Tsuji M., Fukuda R.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli."
    Dartigalongue C., Raina S.
    EMBO J. 17:3968-3980(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS.
  6. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiPPID_ECOLI
AccessioniPrimary (citable) accession number: P0ADY1
Secondary accession number(s): P77241, Q2MBY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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