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P0ADY1

- PPID_ECOLI

UniProt

P0ADY1 - PPID_ECOLI

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Protein

Peptidyl-prolyl cis-trans isomerase D

Gene

ppiD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. Seems to be involved in the folding of outer membrane proteins. Its preference at the P1 position of the peptide substrate is Glu > Leu > Ala > His > Val > Phe > Ile > Gly > Lys > Thr.

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB-KW

GO - Biological processi

  1. protein folding Source: UniProtKB-KW
  2. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:G6242-MONOMER.
ECOL316407:JW0431-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase D (EC:5.2.1.8)
Short name:
PPIase D
Alternative name(s):
Rotamase D
Gene namesi
Name:ppiD
Synonyms:ybaU
Ordered Locus Names:b0441, JW0431
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13249. ppiD.

Subcellular locationi

Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication; Periplasmic side 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence AnalysisAdd
BLAST
Topological domaini37 – 623587PeriplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi312 – 3121G → A or R: Loss of activity. 1 Publication
Mutagenesisi313 – 3131G → A or R: Loss of activity. 1 Publication
Mutagenesisi347 – 3471G → A: Loss of activity. 1 Publication
Mutagenesisi350 – 3501I → A or F: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Peptidyl-prolyl cis-trans isomerase DPRO_0000193423Add
BLAST

Proteomic databases

PaxDbiP0ADY1.
PRIDEiP0ADY1.

Expressioni

Inductioni

By heat shock.

Gene expression databases

GenevestigatoriP0ADY1.

Interactioni

Subunit structurei

Has been isolated as a homodimer and homotrimer from inner membrane preparations.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-562001,EBI-562001

Protein-protein interaction databases

DIPiDIP-39902N.
IntActiP0ADY1. 8 interactions.
STRINGi511145.b0441.

Structurei

Secondary structure

1
623
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi268 – 27811Combined sources
Helixi279 – 29113Combined sources
Helixi295 – 3017Combined sources
Helixi306 – 3094Combined sources
Turni310 – 3123Combined sources
Beta strandi313 – 3197Combined sources
Helixi325 – 3284Combined sources
Beta strandi338 – 3447Combined sources
Beta strandi347 – 35711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KGJNMR-A264-357[»]
ProteinModelPortaliP0ADY1.
SMRiP0ADY1. Positions 86-422.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ADY1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini266 – 35590PpiCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PpiC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0760.
HOGENOMiHOG000276975.
InParanoidiP0ADY1.
KOiK03770.
OMAiQNIRDNS.
OrthoDBiEOG6M9DS4.
PhylomeDBiP0ADY1.

Family and domain databases

InterProiIPR000297. PPIase_PpiC.
IPR023058. PPIase_PpiC_CS.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]
PfamiPF13145. Rotamase_2. 1 hit.
[Graphical view]
SUPFAMiSSF109998. SSF109998. 2 hits.
PROSITEiPS01096. PPIC_PPIASE_1. 1 hit.
PS50198. PPIC_PPIASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ADY1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMDSLRTAAN SLVLKIIFGI IIVSFILTGV SGYLIGGGNN YAAKVNDQEI
60 70 80 90 100
SRGQFENAFN SERNRMQQQL GDQYSELAAN EGYMKTLRQQ VLNRLIDEAL
110 120 130 140 150
LDQYARELKL GISDEQVKQA IFATPAFQVD GKFDNSRYNG ILNQMGMTAD
160 170 180 190 200
QYAQALRNQL TTQQLINGVA GTDFMLKGET DELAALVAQQ RVVREATIDV
210 220 230 240 250
NALAAKQPVT EQEIASYYEQ NKNNFMTPEQ FRVSYIKLDA ATMQQPVSDA
260 270 280 290 300
DIQSYYDQHQ DQFTQPQRTR YSIIQTKTED EAKAVLDELN KGGDFAALAK
310 320 330 340 350
EKSADIISAR NGGDMGWLED ATIPDELKNA GLKEKGQLSG VIKSSVGFLI
360 370 380 390 400
VRLDDIQPAK VKSLDEVRDD IAAKVKHEKA LDAYYALQQK VSDAASNDTE
410 420 430 440 450
SLAGAEQAAG VKATQTGWFS KDNLPEELNF KPVADAIFNG GLVGENGAPG
460 470 480 490 500
INSDIITVDG DRAFVLRISE HKPEAVKPLA DVQEQVKALV QHNKAEQQAK
510 520 530 540 550
VDAEKLLVDL KAGKGAEAMQ AAGLKFGEPK TLSRSGRDPI SQAAFALPLP
560 570 580 590 600
AKDKPSYGMA TDMQGNVVLL ALDEVKQGSM PEDQKKAMVQ GITQNNAQIV
610 620
FEALMSNLRK EAKIKIGDAL EQQ
Length:623
Mass (Da):68,150
Last modified:December 6, 2005 - v1
Checksum:i0F646F687114A387
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA. Translation: BAA11645.1.
U82664 Genomic DNA. Translation: AAB40197.1.
U00096 Genomic DNA. Translation: AAC73544.1.
AP009048 Genomic DNA. Translation: BAE76221.1.
PIRiA64774.
RefSeqiNP_414975.1. NC_000913.3.
YP_488733.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73544; AAC73544; b0441.
BAE76221; BAE76221; BAE76221.
GeneIDi12931738.
946056.
KEGGiecj:Y75_p0429.
eco:b0441.
PATRICi32116035. VBIEscCol129921_0459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA. Translation: BAA11645.1 .
U82664 Genomic DNA. Translation: AAB40197.1 .
U00096 Genomic DNA. Translation: AAC73544.1 .
AP009048 Genomic DNA. Translation: BAE76221.1 .
PIRi A64774.
RefSeqi NP_414975.1. NC_000913.3.
YP_488733.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KGJ NMR - A 264-357 [» ]
ProteinModelPortali P0ADY1.
SMRi P0ADY1. Positions 86-422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-39902N.
IntActi P0ADY1. 8 interactions.
STRINGi 511145.b0441.

Proteomic databases

PaxDbi P0ADY1.
PRIDEi P0ADY1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73544 ; AAC73544 ; b0441 .
BAE76221 ; BAE76221 ; BAE76221 .
GeneIDi 12931738.
946056.
KEGGi ecj:Y75_p0429.
eco:b0441.
PATRICi 32116035. VBIEscCol129921_0459.

Organism-specific databases

EchoBASEi EB3038.
EcoGenei EG13249. ppiD.

Phylogenomic databases

eggNOGi COG0760.
HOGENOMi HOG000276975.
InParanoidi P0ADY1.
KOi K03770.
OMAi QNIRDNS.
OrthoDBi EOG6M9DS4.
PhylomeDBi P0ADY1.

Enzyme and pathway databases

BioCyci EcoCyc:G6242-MONOMER.
ECOL316407:JW0431-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ADY1.
PROi P0ADY1.

Gene expression databases

Genevestigatori P0ADY1.

Family and domain databases

InterProi IPR000297. PPIase_PpiC.
IPR023058. PPIase_PpiC_CS.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view ]
Pfami PF13145. Rotamase_2. 1 hit.
[Graphical view ]
SUPFAMi SSF109998. SSF109998. 2 hits.
PROSITEi PS01096. PPIC_PPIASE_1. 1 hit.
PS50198. PPIC_PPIASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Hatada E., Ohmori H., Qiao Y., Tsuji M., Fukuda R.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli."
    Dartigalongue C., Raina S.
    EMBO J. 17:3968-3980(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS.
  6. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiPPID_ECOLI
AccessioniPrimary (citable) accession number: P0ADY1
Secondary accession number(s): P77241, Q2MBY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3