Ribosomal RNA small subunit methyltransferase D - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0ADX9 (RSMD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase D
EC=2.1.1.171

Alternative name(s):
16S rRNA m2G966 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase

Gene names

Name:	rsmD
Synonyms:	yhhF
Ordered Locus Names:	b3465, JW3430

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle. Ref.5
Catalytic activity	S-adenosyl-L-methionine + guanosine(966) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(966) in 16S rRNA.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmD family.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA base methylation Inferred from mutant phenotype Ref.5. Source: EcoCyc
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaK	P0A6Y8	1	EBI-561207,EBI-542092
tufA	P0CE47	1	EBI-561207,EBI-301077

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

Ribosomal RNA small subunit methyltransferase D

PRO_0000169548

Secondary structure

198

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ADX9 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 04FB9D9DAF972605
Show »

FASTA

198

21,678

        10         20         30         40         50         60 
MKKPNHSGSG QIRIIGGQWR GRKLPVPDSP GLRPTTDRVR ETLFNWLAPV IVDAQCLDCF 

        70         80         90        100        110        120 
AGSGALGLEA LSRYAAGATL IEMDRAVSQQ LIKNLATLKA GNARVVNSNA MSFLAQKGTP 

       130        140        150        160        170        180 
HNIVFVDPPF RRGLLEETIN LLEDNGWLAD EALIYVESEV ENGLPTVPAN WSLHREKVAG 

       190 
QVAYRLYQRE AQGESDAD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new cell division operon in Escherichia coli." Gill D.R., Hatfull G.F., Salmond G.P.C. Mol. Gen. Genet. 205:134-145(1986) [PubMed: 3025556] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure." Lesnyak D.V., Osipiuk J., Skarina T., Sergiev P.V., Bogdanov A.A., Edwards A., Savchenko A., Joachimiak A., Dontsova O.A. J. Biol. Chem. 282:5880-5887(2007) [PubMed: 17189261] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS), FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04398 Genomic DNA. Translation: CAA27983.1.
U00039 Genomic DNA. Translation: AAB18440.1.
U00096 Genomic DNA. Translation: AAC76490.1.
AP009048 Genomic DNA. Translation: BAE77828.1.

PIR

QQECX3. S03129.

RefSeq

NP_417922.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FPO	X-ray	2.05	A/B/C/D/E/F	1-198	[»]

ProteinModelPortal

P0ADX9.

SMR

P0ADX9. Positions 10-192.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48275N.

IntAct

P0ADX9. 6 interactions.

MINT

MINT-1267285.

2D gel databases

SWISS-2DPAGE

P0ADX9.

ECO2DBASE

H023.2. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004909; EBESCP00000004909; EBESCG00000004008.
EBESCT00000017653; EBESCP00000016944; EBESCG00000016709.

GeneID

947977.

GenomeReviews

Gene locus JW3430 in contig AP009048_GR.
Gene locus b3465 in contig U00096_GR.

KEGG

ecj:JW3430.
eco:b3465.

PATRIC

32122374. VBIEscCol129921_3564.

Organism-specific databases

EchoBASE

EB0339.

EcoGene

EG10343. rsmD.

Phylogenomic databases

eggNOG

COG0742.

GeneTree

EBGT00050000011512.

HOGENOM

HBG706795.

OMA

ISGQWRS.

PhylomeDB

P0ADX9.

ProtClustDB

PRK10909.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10343-MONOMER.
MetaCyc:EG10343-MONOMER.

Gene expression databases

Genevestigator

P0ADX9.

Family and domain databases

InterPro

IPR002052. DNA_methylase_N6_adenine_CS.
IPR004398. RNA_MeTrfase_RsmD.
[Graphical view]

K08316.

Pfam

PF03602. Cons_hypoth95. 1 hit.
[Graphical view]

PIRSF

PIRSF004553. CHP00095. 1 hit.

TIGRFAMs

TIGR00095. TIGR00095. 1 hit.

PROSITE

PS00092. N6_MTASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RSMD_ECOLI

Accession

Primary (citable) accession number: P0ADX9
Secondary accession number(s): P10120, Q2M7C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents