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P0ADV9 (LPTC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipopolysaccharide export system protein LptC
Gene names
Name:lptC
Synonyms:yrbK
Ordered Locus Names:b3199, JW3166
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length191 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. Facilitates the transfer of LPS from the inner membrane to the periplasmic protein LptA. Could be a docking site for LptA. Ref.5 Ref.6 Ref.8 Ref.9 Ref.11

Subunit structure

Component of the lipopolysaccharide transport and assembly complex. Interacts with LptA and the LptBFG transporter complex. When overexpressed, can form homodimers in vivo. Ref.4 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Cell inner membrane; Single-pass membrane protein Ref.6 Ref.11.

Disruption phenotype

Leads to irreversible cell damage, growth arrest and an extreme sensitivity to SDS. Ref.5

Miscellaneous

Interacts with the LptBFG ABC transporter complex, but does not affect its ATPase activity (Ref.4).

Sequence similarities

Belongs to the LptC family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself4EBI-1131969,EBI-1131969
lptAP0ADV13EBI-1131969,EBI-1132001

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 191191Lipopolysaccharide export system protein LptC HAMAP-Rule MF_01915
PRO_0000169470

Regions

Transmembrane7 – 2519Helical; Potential

Experimental info

Mutagenesis561G → V: Impairs LPS transport. Does not abolish interaction with LptA. Ref.9
Mutagenesis1531G → R: Impairs LPS transport. Fails to interacts with LptA. Ref.9
Mutagenesis177 – 19115Missing: Fails to interact with LptA. Ref.9
Sequence conflict21S → G AA sequence Ref.4

Secondary structure

.......................... 191
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ADV9 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: F6BAE79EA232CD95

FASTA19121,703
        10         20         30         40         50         60 
MSKARRWVII VLSLAVLVMI GINMAEKDDT AQVVVNNNDP TYKSEHTDTL VYNPEGALSY 

        70         80         90        100        110        120 
RLIAQHVEYY SDQAVSWFTQ PVLTTFDKDK IPTWSVKADK AKLTNDRMLY LYGHVEVNAL 

       130        140        150        160        170        180 
VPDSQLRRIT TDNAQINLVT QDVTSEDLVT LYGTTFNSSG LKMRGNLRSK NAELIEKVRT 

       190 
SYEIQNKQTQ P 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant."
Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J.
J. Biol. Chem. 270:4822-4839(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 136-191.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides."
Narita S., Tokuda H.
FEBS Lett. 583:2160-2164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-5, INTERACTION WITH LPTBFG.
[5]"Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus."
Sperandeo P., Pozzi C., Deho G., Polissi A.
Res. Microbiol. 157:547-558(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN LPS BIOSYNTHESIS, DISRUPTION PHENOTYPE.
[6]"Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli."
Sperandeo P., Lau F.K., Carpentieri A., De Castro C., Molinaro A., Deho G., Silhavy T.J., Polissi A.
J. Bacteriol. 190:4460-4469(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, SUBCELLULAR LOCATION.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[7]"Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex."
Chng S.S., Gronenberg L.S., Kahne D.
Biochemistry 49:4565-4567(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH LPTBFG.
[8]"Characterization of interactions between LPS transport proteins of the Lpt system."
Bowyer A., Baardsnes J., Ajamian E., Zhang L., Cygler M.
Biochem. Biophys. Res. Commun. 404:1093-1098(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH LPTA.
[9]"New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex."
Sperandeo P., Villa R., Martorana A.M., Samalikova M., Grandori R., Deho G., Polissi A.
J. Bacteriol. 193:1042-1053(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH LPTA, MUTAGENESIS OF GLY-56; GLY-153 AND 177-LYS--PRO-191.
[10]"Regulated assembly of the transenvelope protein complex required for lipopolysaccharide export."
Freinkman E., Okuda S., Ruiz N., Kahne D.
Biochemistry 51:4800-4806(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH LPTA.
[11]"Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coli."
Tran A.X., Dong C., Whitfield C.
J. Biol. Chem. 285:33529-33539(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 23-191, FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18997 Genomic DNA. Translation: AAA58001.1.
U00096 Genomic DNA. Translation: AAC76231.1.
AP009048 Genomic DNA. Translation: BAE77243.1.
U12684 Genomic DNA. No translation available.
PIRA65111.
RefSeqNP_417666.1. NC_000913.3.
YP_491384.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3MY2X-ray2.20A23-191[»]
4B54X-ray2.80A/B24-191[»]
ProteinModelPortalP0ADV9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12912N.
IntActP0ADV9. 3 interactions.
MINTMINT-7137017.
STRING511145.b3199.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76231; AAC76231; b3199.
BAE77243; BAE77243; BAE77243.
GeneID12930285.
947722.
KEGGecj:Y75_p3119.
eco:b3199.
PATRIC32121818. VBIEscCol129921_3293.

Organism-specific databases

EchoBASEEB2657.
EcoGeneEG12806. lptC.

Phylogenomic databases

eggNOGCOG3117.
HOGENOMHOG000125239.
KOK11719.
OMAWFTQPVM.
OrthoDBEOG6W7212.

Enzyme and pathway databases

BioCycEcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Gene expression databases

GenevestigatorP0ADV9.

Family and domain databases

HAMAPMF_01915. LPS_assembly_LptC.
InterProIPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view]
PfamPF06835. LptC. 1 hit.
[Graphical view]
PIRSFPIRSF028513. LptC. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0ADV9.
PROP0ADV9.

Entry information

Entry nameLPTC_ECOLI
AccessionPrimary (citable) accession number: P0ADV9
Secondary accession number(s): P45397, Q2M913
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene