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Protein

Lipopolysaccharide export system protein LptC

Gene

lptC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. Facilitates the transfer of LPS from the inner membrane to the periplasmic protein LptA. Could be a docking site for LptA.UniRule annotation5 Publications

GO - Molecular functioni

  • glycolipid transporter activity Source: EcoCyc
  • lipopolysaccharide transmembrane transporter activity Source: InterPro

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: UniProtKB-HAMAP
  • lipopolysaccharide transport Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide export system protein LptCUniRule annotation
Gene namesi
Name:lptCUniRule annotation
Synonyms:yrbK
Ordered Locus Names:b3199, JW3166
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12806. lptC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 25HelicalUniRule annotationAdd BLAST19

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to irreversible cell damage, growth arrest and an extreme sensitivity to SDS.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56G → V: Impairs LPS transport. Does not abolish interaction with LptA. 1 Publication1
Mutagenesisi153G → R: Impairs LPS transport. Fails to interacts with LptA. 1 Publication1
Mutagenesisi177 – 191Missing : Fails to interact with LptA. 1 PublicationAdd BLAST15

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694701 – 191Lipopolysaccharide export system protein LptCAdd BLAST191

Proteomic databases

PaxDbiP0ADV9.
PRIDEiP0ADV9.

Interactioni

Subunit structurei

Component of the lipopolysaccharide transport and assembly complex. Interacts with LptA and the LptBFG transporter complex. When overexpressed, can form homodimers in vivo.UniRule annotation5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1131969,EBI-1131969
lptAP0ADV13EBI-1131969,EBI-1132001

Protein-protein interaction databases

BioGridi4259279. 264 interactors.
DIPiDIP-12912N.
IntActiP0ADV9. 3 interactors.
MINTiMINT-7137017.
STRINGi511145.b3199.

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 63Combined sources4
Beta strandi65 – 70Combined sources6
Turni71 – 74Combined sources4
Beta strandi75 – 80Combined sources6
Beta strandi82 – 86Combined sources5
Beta strandi92 – 97Combined sources6
Beta strandi99 – 103Combined sources5
Beta strandi107 – 119Combined sources13
Beta strandi121 – 123Combined sources3
Beta strandi125 – 137Combined sources13
Turni138 – 140Combined sources3
Beta strandi142 – 155Combined sources14
Beta strandi157 – 166Combined sources10
Turni167 – 170Combined sources4
Beta strandi171 – 180Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MY2X-ray2.20A23-191[»]
4B54X-ray2.80A/B24-191[»]
ProteinModelPortaliP0ADV9.
SMRiP0ADV9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ADV9.

Family & Domainsi

Sequence similaritiesi

Belongs to the LptC family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108P1K. Bacteria.
COG3117. LUCA.
HOGENOMiHOG000125239.
KOiK11719.
OMAiWFTQPVM.

Family and domain databases

HAMAPiMF_01915. LPS_assembly_LptC. 1 hit.
InterProiIPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view]
PfamiPF06835. LptC. 1 hit.
[Graphical view]
PIRSFiPIRSF028513. LptC. 1 hit.
TIGRFAMsiTIGR04409. LptC_YrbK. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ADV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKARRWVII VLSLAVLVMI GINMAEKDDT AQVVVNNNDP TYKSEHTDTL
60 70 80 90 100
VYNPEGALSY RLIAQHVEYY SDQAVSWFTQ PVLTTFDKDK IPTWSVKADK
110 120 130 140 150
AKLTNDRMLY LYGHVEVNAL VPDSQLRRIT TDNAQINLVT QDVTSEDLVT
160 170 180 190
LYGTTFNSSG LKMRGNLRSK NAELIEKVRT SYEIQNKQTQ P
Length:191
Mass (Da):21,703
Last modified:December 6, 2005 - v1
Checksum:iF6BAE79EA232CD95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → G AA sequence (PubMed:19500581).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58001.1.
U00096 Genomic DNA. Translation: AAC76231.1.
AP009048 Genomic DNA. Translation: BAE77243.1.
U12684 Genomic DNA. No translation available.
PIRiA65111.
RefSeqiNP_417666.1. NC_000913.3.
WP_000030537.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76231; AAC76231; b3199.
BAE77243; BAE77243; BAE77243.
GeneIDi947722.
KEGGiecj:JW3166.
eco:b3199.
PATRICi32121818. VBIEscCol129921_3293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58001.1.
U00096 Genomic DNA. Translation: AAC76231.1.
AP009048 Genomic DNA. Translation: BAE77243.1.
U12684 Genomic DNA. No translation available.
PIRiA65111.
RefSeqiNP_417666.1. NC_000913.3.
WP_000030537.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MY2X-ray2.20A23-191[»]
4B54X-ray2.80A/B24-191[»]
ProteinModelPortaliP0ADV9.
SMRiP0ADV9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259279. 264 interactors.
DIPiDIP-12912N.
IntActiP0ADV9. 3 interactors.
MINTiMINT-7137017.
STRINGi511145.b3199.

Proteomic databases

PaxDbiP0ADV9.
PRIDEiP0ADV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76231; AAC76231; b3199.
BAE77243; BAE77243; BAE77243.
GeneIDi947722.
KEGGiecj:JW3166.
eco:b3199.
PATRICi32121818. VBIEscCol129921_3293.

Organism-specific databases

EchoBASEiEB2657.
EcoGeneiEG12806. lptC.

Phylogenomic databases

eggNOGiENOG4108P1K. Bacteria.
COG3117. LUCA.
HOGENOMiHOG000125239.
KOiK11719.
OMAiWFTQPVM.

Enzyme and pathway databases

BioCyciEcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ADV9.
PROiP0ADV9.

Family and domain databases

HAMAPiMF_01915. LPS_assembly_LptC. 1 hit.
InterProiIPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view]
PfamiPF06835. LptC. 1 hit.
[Graphical view]
PIRSFiPIRSF028513. LptC. 1 hit.
TIGRFAMsiTIGR04409. LptC_YrbK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPTC_ECOLI
AccessioniPrimary (citable) accession number: P0ADV9
Secondary accession number(s): P45397, Q2M913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Interacts with the LptBFG ABC transporter complex, but does not affect its ATPase activity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.