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Protein

Lipopolysaccharide export system protein LptC

Gene

lptC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. Facilitates the transfer of LPS from the inner membrane to the periplasmic protein LptA. Could be a docking site for LptA.UniRule annotation5 Publications

GO - Molecular functioni

  • glycolipid transporter activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • lipopolysaccharide transmembrane transporter activity Source: InterPro

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: UniProtKB-HAMAP
  • lipopolysaccharide transport Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide export system protein LptCUniRule annotation
Gene namesi
Name:lptCUniRule annotation
Synonyms:yrbK
Ordered Locus Names:b3199, JW3166
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12806. lptC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2519HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to irreversible cell damage, growth arrest and an extreme sensitivity to SDS.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561G → V: Impairs LPS transport. Does not abolish interaction with LptA. 1 Publication
Mutagenesisi153 – 1531G → R: Impairs LPS transport. Fails to interacts with LptA. 1 Publication
Mutagenesisi177 – 19115Missing : Fails to interact with LptA. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Lipopolysaccharide export system protein LptCPRO_0000169470Add
BLAST

Proteomic databases

PaxDbiP0ADV9.

Interactioni

Subunit structurei

Component of the lipopolysaccharide transport and assembly complex. Interacts with LptA and the LptBFG transporter complex. When overexpressed, can form homodimers in vivo.UniRule annotation5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1131969,EBI-1131969
lptAP0ADV13EBI-1131969,EBI-1132001

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259279. 264 interactions.
DIPiDIP-12912N.
IntActiP0ADV9. 3 interactions.
MINTiMINT-7137017.
STRINGi511145.b3199.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi60 – 634Combined sources
Beta strandi65 – 706Combined sources
Turni71 – 744Combined sources
Beta strandi75 – 806Combined sources
Beta strandi82 – 865Combined sources
Beta strandi92 – 976Combined sources
Beta strandi99 – 1035Combined sources
Beta strandi107 – 11913Combined sources
Beta strandi121 – 1233Combined sources
Beta strandi125 – 13713Combined sources
Turni138 – 1403Combined sources
Beta strandi142 – 15514Combined sources
Beta strandi157 – 16610Combined sources
Turni167 – 1704Combined sources
Beta strandi171 – 18010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MY2X-ray2.20A23-191[»]
4B54X-ray2.80A/B24-191[»]
ProteinModelPortaliP0ADV9.
SMRiP0ADV9. Positions 59-184.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ADV9.

Family & Domainsi

Sequence similaritiesi

Belongs to the LptC family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108P1K. Bacteria.
COG3117. LUCA.
HOGENOMiHOG000125239.
KOiK11719.
OMAiWFTQPVM.

Family and domain databases

HAMAPiMF_01915. LPS_assembly_LptC. 1 hit.
InterProiIPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view]
PfamiPF06835. LptC. 1 hit.
[Graphical view]
PIRSFiPIRSF028513. LptC. 1 hit.
TIGRFAMsiTIGR04409. LptC_YrbK. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ADV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKARRWVII VLSLAVLVMI GINMAEKDDT AQVVVNNNDP TYKSEHTDTL
60 70 80 90 100
VYNPEGALSY RLIAQHVEYY SDQAVSWFTQ PVLTTFDKDK IPTWSVKADK
110 120 130 140 150
AKLTNDRMLY LYGHVEVNAL VPDSQLRRIT TDNAQINLVT QDVTSEDLVT
160 170 180 190
LYGTTFNSSG LKMRGNLRSK NAELIEKVRT SYEIQNKQTQ P
Length:191
Mass (Da):21,703
Last modified:December 6, 2005 - v1
Checksum:iF6BAE79EA232CD95
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → G AA sequence (PubMed:19500581).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58001.1.
U00096 Genomic DNA. Translation: AAC76231.1.
AP009048 Genomic DNA. Translation: BAE77243.1.
U12684 Genomic DNA. No translation available.
PIRiA65111.
RefSeqiNP_417666.1. NC_000913.3.
WP_000030537.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76231; AAC76231; b3199.
BAE77243; BAE77243; BAE77243.
GeneIDi947722.
KEGGiecj:JW3166.
eco:b3199.
PATRICi32121818. VBIEscCol129921_3293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58001.1.
U00096 Genomic DNA. Translation: AAC76231.1.
AP009048 Genomic DNA. Translation: BAE77243.1.
U12684 Genomic DNA. No translation available.
PIRiA65111.
RefSeqiNP_417666.1. NC_000913.3.
WP_000030537.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MY2X-ray2.20A23-191[»]
4B54X-ray2.80A/B24-191[»]
ProteinModelPortaliP0ADV9.
SMRiP0ADV9. Positions 59-184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259279. 264 interactions.
DIPiDIP-12912N.
IntActiP0ADV9. 3 interactions.
MINTiMINT-7137017.
STRINGi511145.b3199.

Proteomic databases

PaxDbiP0ADV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76231; AAC76231; b3199.
BAE77243; BAE77243; BAE77243.
GeneIDi947722.
KEGGiecj:JW3166.
eco:b3199.
PATRICi32121818. VBIEscCol129921_3293.

Organism-specific databases

EchoBASEiEB2657.
EcoGeneiEG12806. lptC.

Phylogenomic databases

eggNOGiENOG4108P1K. Bacteria.
COG3117. LUCA.
HOGENOMiHOG000125239.
KOiK11719.
OMAiWFTQPVM.

Enzyme and pathway databases

BioCyciEcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ADV9.
PROiP0ADV9.

Family and domain databases

HAMAPiMF_01915. LPS_assembly_LptC. 1 hit.
InterProiIPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view]
PfamiPF06835. LptC. 1 hit.
[Graphical view]
PIRSFiPIRSF028513. LptC. 1 hit.
TIGRFAMsiTIGR04409. LptC_YrbK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPTC_ECOLI
AccessioniPrimary (citable) accession number: P0ADV9
Secondary accession number(s): P45397, Q2M913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Interacts with the LptBFG ABC transporter complex, but does not affect its ATPase activity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.