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P0ADV9

- LPTC_ECOLI

UniProt

P0ADV9 - LPTC_ECOLI

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Protein

Lipopolysaccharide export system protein LptC

Gene

lptC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. Facilitates the transfer of LPS from the inner membrane to the periplasmic protein LptA. Could be a docking site for LptA.5 PublicationsUniRule annotation

GO - Molecular functioni

  1. glycolipid transporter activity Source: EcoCyc
  2. identical protein binding Source: IntAct
  3. lipopolysaccharide transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. glycolipid transport Source: GOC
  2. lipopolysaccharide transport Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide export system protein LptCUniRule annotation
Gene namesi
Name:lptCUniRule annotation
Synonyms:yrbK
Ordered Locus Names:b3199, JW3166
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12806. lptC.

Subcellular locationi

Cell inner membrane 2 PublicationsUniRule annotation; Single-pass membrane protein 2 PublicationsUniRule annotation

GO - Cellular componenti

  1. integral component of membrane Source: EcoCyc
  2. integral component of plasma membrane Source: EcoCyc
  3. outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to irreversible cell damage, growth arrest and an extreme sensitivity to SDS.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561G → V: Impairs LPS transport. Does not abolish interaction with LptA. 1 Publication
Mutagenesisi153 – 1531G → R: Impairs LPS transport. Fails to interacts with LptA. 1 Publication
Mutagenesisi177 – 19115Missing: Fails to interact with LptA. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Lipopolysaccharide export system protein LptCPRO_0000169470Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0ADV9.

Interactioni

Subunit structurei

Component of the lipopolysaccharide transport and assembly complex. Interacts with LptA and the LptBFG transporter complex. When overexpressed, can form homodimers in vivo.5 PublicationsUniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1131969,EBI-1131969
lptAP0ADV13EBI-1131969,EBI-1132001

Protein-protein interaction databases

DIPiDIP-12912N.
IntActiP0ADV9. 3 interactions.
MINTiMINT-7137017.
STRINGi511145.b3199.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi60 – 634
Beta strandi65 – 706
Turni71 – 744
Beta strandi75 – 806
Beta strandi82 – 865
Beta strandi92 – 976
Beta strandi99 – 1035
Beta strandi107 – 11913
Beta strandi121 – 1233
Beta strandi125 – 13713
Turni138 – 1403
Beta strandi142 – 15514
Beta strandi157 – 16610
Turni167 – 1704
Beta strandi171 – 18010

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MY2X-ray2.20A23-191[»]
4B54X-ray2.80A/B24-191[»]
ProteinModelPortaliP0ADV9.
SMRiP0ADV9. Positions 59-184.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ADV9.

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2519HelicalUniRule annotationAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the LptC family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3117.
HOGENOMiHOG000125239.
KOiK11719.
OMAiWFTQPVM.
OrthoDBiEOG6W7212.

Family and domain databases

HAMAPiMF_01915. LPS_assembly_LptC.
InterProiIPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view]
PfamiPF06835. LptC. 1 hit.
[Graphical view]
PIRSFiPIRSF028513. LptC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ADV9 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKARRWVII VLSLAVLVMI GINMAEKDDT AQVVVNNNDP TYKSEHTDTL
60 70 80 90 100
VYNPEGALSY RLIAQHVEYY SDQAVSWFTQ PVLTTFDKDK IPTWSVKADK
110 120 130 140 150
AKLTNDRMLY LYGHVEVNAL VPDSQLRRIT TDNAQINLVT QDVTSEDLVT
160 170 180 190
LYGTTFNSSG LKMRGNLRSK NAELIEKVRT SYEIQNKQTQ P
Length:191
Mass (Da):21,703
Last modified:December 6, 2005 - v1
Checksum:iF6BAE79EA232CD95
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → G AA sequence (PubMed:19500581)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18997 Genomic DNA. Translation: AAA58001.1.
U00096 Genomic DNA. Translation: AAC76231.1.
AP009048 Genomic DNA. Translation: BAE77243.1.
U12684 Genomic DNA. No translation available.
PIRiA65111.
RefSeqiNP_417666.1. NC_000913.3.
YP_491384.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76231; AAC76231; b3199.
BAE77243; BAE77243; BAE77243.
GeneIDi12930285.
947722.
KEGGiecj:Y75_p3119.
eco:b3199.
PATRICi32121818. VBIEscCol129921_3293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18997 Genomic DNA. Translation: AAA58001.1 .
U00096 Genomic DNA. Translation: AAC76231.1 .
AP009048 Genomic DNA. Translation: BAE77243.1 .
U12684 Genomic DNA. No translation available.
PIRi A65111.
RefSeqi NP_417666.1. NC_000913.3.
YP_491384.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MY2 X-ray 2.20 A 23-191 [» ]
4B54 X-ray 2.80 A/B 24-191 [» ]
ProteinModelPortali P0ADV9.
SMRi P0ADV9. Positions 59-184.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12912N.
IntActi P0ADV9. 3 interactions.
MINTi MINT-7137017.
STRINGi 511145.b3199.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76231 ; AAC76231 ; b3199 .
BAE77243 ; BAE77243 ; BAE77243 .
GeneIDi 12930285.
947722.
KEGGi ecj:Y75_p3119.
eco:b3199.
PATRICi 32121818. VBIEscCol129921_3293.

Organism-specific databases

EchoBASEi EB2657.
EcoGenei EG12806. lptC.

Phylogenomic databases

eggNOGi COG3117.
HOGENOMi HOG000125239.
KOi K11719.
OMAi WFTQPVM.
OrthoDBi EOG6W7212.

Enzyme and pathway databases

BioCyci EcoCyc:G7664-MONOMER.
ECOL316407:JW3166-MONOMER.
MetaCyc:G7664-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ADV9.
PROi P0ADV9.

Gene expression databases

Genevestigatori P0ADV9.

Family and domain databases

HAMAPi MF_01915. LPS_assembly_LptC.
InterProi IPR010664. LipoPS_assembly_LptC-rel.
IPR026265. LptC.
[Graphical view ]
Pfami PF06835. LptC. 1 hit.
[Graphical view ]
PIRSFi PIRSF028513. LptC. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant."
    Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J.
    J. Biol. Chem. 270:4822-4839(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 136-191.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides."
    Narita S., Tokuda H.
    FEBS Lett. 583:2160-2164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-5, INTERACTION WITH LPTBFG.
  5. "Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus."
    Sperandeo P., Pozzi C., Deho G., Polissi A.
    Res. Microbiol. 157:547-558(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LPS BIOSYNTHESIS, DISRUPTION PHENOTYPE.
  6. "Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli."
    Sperandeo P., Lau F.K., Carpentieri A., De Castro C., Molinaro A., Deho G., Silhavy T.J., Polissi A.
    J. Bacteriol. 190:4460-4469(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, SUBCELLULAR LOCATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  7. "Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex."
    Chng S.S., Gronenberg L.S., Kahne D.
    Biochemistry 49:4565-4567(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH LPTBFG.
  8. "Characterization of interactions between LPS transport proteins of the Lpt system."
    Bowyer A., Baardsnes J., Ajamian E., Zhang L., Cygler M.
    Biochem. Biophys. Res. Commun. 404:1093-1098(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LPTA.
  9. "New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex."
    Sperandeo P., Villa R., Martorana A.M., Samalikova M., Grandori R., Deho G., Polissi A.
    J. Bacteriol. 193:1042-1053(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH LPTA, MUTAGENESIS OF GLY-56; GLY-153 AND 177-LYS--PRO-191.
  10. "Regulated assembly of the transenvelope protein complex required for lipopolysaccharide export."
    Freinkman E., Okuda S., Ruiz N., Kahne D.
    Biochemistry 51:4800-4806(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH LPTA.
  11. "Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coli."
    Tran A.X., Dong C., Whitfield C.
    J. Biol. Chem. 285:33529-33539(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 23-191, FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiLPTC_ECOLI
AccessioniPrimary (citable) accession number: P0ADV9
Secondary accession number(s): P45397, Q2M913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Interacts with the LptBFG ABC transporter complex, but does not affect its ATPase activity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3