Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipopolysaccharide export system protein LptA

Gene

lptA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.UniRule annotation5 Publications

GO - Molecular functioni

  • glycolipid transporter activity Source: EcoCyc
  • lipopolysaccharide binding Source: InterPro

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: UniProtKB-HAMAP
  • lipopolysaccharide transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YHBN-MONOMER.
ECOL316407:JW3167-MONOMER.
MetaCyc:YHBN-MONOMER.

Protein family/group databases

TCDBi1.B.42.1.2. the outer membrane lipopolysaccharide export porin (lps-ep) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide export system protein LptAUniRule annotation
Gene namesi
Name:lptAUniRule annotation
Synonyms:yhbN
Ordered Locus Names:b3200, JW3167
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12618. lptA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Results in an earlier growth arrest and onset of cell lethality.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 361I → D or E: No change in activity. 1 Publication
Mutagenesisi38 – 381I → D: Decrease in activity. 1 Publication
Mutagenesisi38 – 381I → E: No change in activity. 1 Publication
Mutagenesisi76 – 761R → D or E: No change in activity. 1 Publication
Mutagenesisi95 – 951F → A: No change in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727UniRule annotation1 PublicationAdd
BLAST
Chaini28 – 185158Lipopolysaccharide export system protein LptAPRO_0000013914Add
BLAST

Proteomic databases

EPDiP0ADV1.
PaxDbiP0ADV1.
PRIDEiP0ADV1.

Expressioni

Inductioni

Transcriptionally regulated by sigma-E factor.1 Publication

Interactioni

Subunit structurei

Component of the lipopolysaccharide transport and assembly complex. Can form head-to-tail homodimers and oligomers. Interacts with LptC, LptD and with the lipid A domain of LPS.UniRule annotation7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
lptCP0ADV93EBI-1132001,EBI-1131969
lptDP315543EBI-1132001,EBI-549369

Protein-protein interaction databases

BioGridi4259280. 236 interactions.
DIPiDIP-12262N.
IntActiP0ADV1. 5 interactions.
STRINGi511145.b3200.

Structurei

Secondary structure

1
185
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 335Combined sources
Beta strandi36 – 4611Combined sources
Turni47 – 504Combined sources
Beta strandi51 – 6212Combined sources
Beta strandi65 – 7511Combined sources
Beta strandi85 – 895Combined sources
Beta strandi94 – 974Combined sources
Beta strandi105 – 1084Combined sources
Beta strandi110 – 1156Combined sources
Helixi116 – 1183Combined sources
Beta strandi120 – 13112Combined sources
Beta strandi134 – 14411Combined sources
Turni145 – 1484Combined sources
Beta strandi149 – 1535Combined sources
Beta strandi155 – 1573Combined sources
Beta strandi160 – 1623Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R19X-ray2.16A/B27-185[»]
2R1AX-ray3.26A/B/C/D/E/F/G/H27-185[»]
ProteinModelPortaliP0ADV1.
SMRiP0ADV1. Positions 28-164.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ADV1.

Family & Domainsi

Domaini

The N-terminal domain interacts with LptC, at the inner membrane, and the C-terminal domain interacts with LptD, at the outer membrane.1 Publication

Sequence similaritiesi

Belongs to the LptA family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105WI0. Bacteria.
COG1934. LUCA.
HOGENOMiHOG000264762.
InParanoidiP0ADV1.
KOiK09774.
OMAiNGQKKSN.
PhylomeDBiP0ADV1.

Family and domain databases

HAMAPiMF_01914. LPS_assembly_LptA. 1 hit.
InterProiIPR014340. LptA.
IPR005653. OstA-like_N.
[Graphical view]
PfamiPF03968. OstA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03002. outer_YhbN_LptA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ADV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFKTNKLSL NLVLASSLLA ASIPAFAVTG DTDQPIHIES DQQSLDMQGN
60 70 80 90 100
VVTFTGNVIV TQGTIKINAD KVVVTRPGGE QGKEVIDGYG KPATFYQMQD
110 120 130 140 150
NGKPVEGHAS QMHYELAKDF VVLTGNAYLQ QVDSNIKGDK ITYLVKEQKM
160 170 180
QAFSDKGKRV TTVLVPSQLQ DKNNKGQTPA QKKGN
Length:185
Mass (Da):20,127
Last modified:December 6, 2005 - v1
Checksum:iBA69198479C68DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60161.1.
U18997 Genomic DNA. Translation: AAA58002.1.
U00096 Genomic DNA. Translation: AAC76232.1.
AP009048 Genomic DNA. Translation: BAE77244.1.
PIRiB65111.
RefSeqiNP_417667.1. NC_000913.3.
WP_000669785.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76232; AAC76232; b3200.
BAE77244; BAE77244; BAE77244.
GeneIDi947920.
KEGGiecj:JW3167.
eco:b3200.
PATRICi32121820. VBIEscCol129921_3294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12684 Genomic DNA. Translation: AAB60161.1.
U18997 Genomic DNA. Translation: AAA58002.1.
U00096 Genomic DNA. Translation: AAC76232.1.
AP009048 Genomic DNA. Translation: BAE77244.1.
PIRiB65111.
RefSeqiNP_417667.1. NC_000913.3.
WP_000669785.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R19X-ray2.16A/B27-185[»]
2R1AX-ray3.26A/B/C/D/E/F/G/H27-185[»]
ProteinModelPortaliP0ADV1.
SMRiP0ADV1. Positions 28-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259280. 236 interactions.
DIPiDIP-12262N.
IntActiP0ADV1. 5 interactions.
STRINGi511145.b3200.

Protein family/group databases

TCDBi1.B.42.1.2. the outer membrane lipopolysaccharide export porin (lps-ep) family.

Proteomic databases

EPDiP0ADV1.
PaxDbiP0ADV1.
PRIDEiP0ADV1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76232; AAC76232; b3200.
BAE77244; BAE77244; BAE77244.
GeneIDi947920.
KEGGiecj:JW3167.
eco:b3200.
PATRICi32121820. VBIEscCol129921_3294.

Organism-specific databases

EchoBASEiEB2502.
EcoGeneiEG12618. lptA.

Phylogenomic databases

eggNOGiENOG4105WI0. Bacteria.
COG1934. LUCA.
HOGENOMiHOG000264762.
InParanoidiP0ADV1.
KOiK09774.
OMAiNGQKKSN.
PhylomeDBiP0ADV1.

Enzyme and pathway databases

BioCyciEcoCyc:YHBN-MONOMER.
ECOL316407:JW3167-MONOMER.
MetaCyc:YHBN-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ADV1.
PROiP0ADV1.

Family and domain databases

HAMAPiMF_01914. LPS_assembly_LptA. 1 hit.
InterProiIPR014340. LptA.
IPR005653. OstA-like_N.
[Graphical view]
PfamiPF03968. OstA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03002. outer_YhbN_LptA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPTA_ECOLI
AccessioniPrimary (citable) accession number: P0ADV1
Secondary accession number(s): P38685, Q2M912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.