Lipopolysaccharide export system protein lptA precursor

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P0ADV1 (LPTA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Lipopolysaccharide export system protein lptA

Gene names

Name:	lptA
Synonyms:	yhbN
Ordered Locus Names:	b3200, JW3167

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. May act as a chaperone that facilitates LPS transfer across the aquaeous environment of the periplasm. Interacts specifically with the lipid A domain of LPS. Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Monomer. Ref.8 Ref.9
Subcellular location	Periplasm Ref.6 Ref.8.
Induction	Transcriptionally regulated by sigma-E factor. Ref.6
Disruption phenotype	Results in an earlier growth arrest and onset of cell lethality. Ref.5
Sequence similarities	Belongs to the lptA family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Periplasm
Domain	Signal
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide transport Inferred from mutant phenotype Ref.6. Source: EcoCyc
Cellular component	periplasmic space Inferred from direct assay Ref.6. Source: EcoCyc
Molecular function	glycolipid transporter activity Inferred from mutant phenotype Ref.6. Source: EcoCyc lipopolysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Ref.4

Chain

28 – 185

158

Lipopolysaccharide export system protein lptA

PRO_0000013914

Experimental info

Mutagenesis

I → D or E: No change in activity. Ref.9

Mutagenesis

I → D: Decrease in activity. Ref.9

Mutagenesis

I → E: No change in activity. Ref.9

Mutagenesis

R → D or E: No change in activity. Ref.9

Mutagenesis

F → A: No change in activity. Ref.9

Secondary structure

185

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ADV1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: BA69198479C68DC1
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FASTA

185

20,127

        10         20         30         40         50         60 
MKFKTNKLSL NLVLASSLLA ASIPAFAVTG DTDQPIHIES DQQSLDMQGN VVTFTGNVIV 

        70         80         90        100        110        120 
TQGTIKINAD KVVVTRPGGE QGKEVIDGYG KPATFYQMQD NGKPVEGHAS QMHYELAKDF 

       130        140        150        160        170        180 
VVLTGNAYLQ QVDSNIKGDK ITYLVKEQKM QAFSDKGKRV TTVLVPSQLQ DKNNKGQTPA 


QKKGN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J. J. Biol. Chem. 270:4822-4839(1995) [PubMed: 7876255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-39. Strain: K12 / EMG2.
[5]	"Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus." Sperandeo P., Pozzi C., Deho G., Polissi A. Res. Microbiol. 157:547-558(2006) [PubMed: 16765569] [Abstract] Cited for: FUNCTION IN LPS BIOSYNTHESIS, DISRUPTION PHENOTYPE.
[6]	"Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli." Sperandeo P., Cescutti R., Villa R., Di Benedetto C., Candia D., Deho G., Polissi A. J. Bacteriol. 189:244-253(2007) [PubMed: 17056748] [Abstract] Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, SUBCELLULAR LOCATION, INDUCTION.
[7]	"Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli." Sperandeo P., Lau F.K., Carpentieri A., De Castro C., Molinaro A., Deho G., Silhavy T.J., Polissi A. J. Bacteriol. 190:4460-4469(2008) [PubMed: 18424520] [Abstract] Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[8]	"The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway." Tran A.X., Trent M.S., Whitfield C. J. Biol. Chem. 283:20342-20349(2008) [PubMed: 18480051] [Abstract] Cited for: FUNCTION IN LIPOPOLYSACCHARIDE TRANSPORT, SUBUNIT, SUBCELLULAR LOCATION, LIPID A-BINDING. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[9]	"Novel structure of the conserved Gram-negative lipopolysaccharide transport protein A and mutagenesis analysis." Suits M.D.L., Sperandeo P., Deho G., Polissi A., Jia Z. J. Mol. Biol. 380:476-488(2008) [PubMed: 18534617] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 27-185, SUBUNIT, MUTAGENESIS OF ILE-36; ILE-38; ARG-76 AND PHE-95. Strain: K12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U12684 Genomic DNA. Translation: AAB60161.1.
U18997 Genomic DNA. Translation: AAA58002.1.
U00096 Genomic DNA. Translation: AAC76232.1.
AP009048 Genomic DNA. Translation: BAE77244.1.

PIR

B65111.

RefSeq

NP_417667.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R19	X-ray	2.16	A/B	27-185	[»]
2R1A	X-ray	3.26	A/B/C/D/E/F/G/H	27-185	[»]

ProteinModelPortal

P0ADV1.

SMR

P0ADV1. Positions 28-164.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0ADV1. 3 interactions.

Protein family/group databases

TCDB

1.B.42.1.2. outer membrane lipopolysaccharide export porin (LPS-EP) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001811; EBESCP00000001811; EBESCG00000001495.
EBESCT00000017131; EBESCP00000016422; EBESCG00000016190.

GeneID

947920.

GenomeReviews

Gene locus JW3167 in contig AP009048_GR.
Gene locus b3200 in contig U00096_GR.

KEGG

ecj:JW3167.
eco:b3200.

PATRIC

32121820. VBIEscCol129921_3294.

Organism-specific databases

EchoBASE

EB2502.

EcoGene

EG12618. lptA.

Phylogenomic databases

eggNOG

COG1934.

GeneTree

EBGT00050000009111.

HOGENOM

HBG701445.

OMA

VTFTDNV.

PhylomeDB

P0ADV1.

ProtClustDB

PRK10894.

Enzyme and pathway databases

BioCyc

EcoCyc:YHBN-MONOMER.

Gene expression databases

Genevestigator

P0ADV1.

Family and domain databases

InterPro

IPR014340. LipoPS_assembly_LptA.
IPR005653. OstA-like_N.
[Graphical view]

K09774.

Pfam

PF03968. OstA. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03002. Outer_YhbN_LptA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

LPTA_ECOLI

Accession

Primary (citable) accession number: P0ADV1
Secondary accession number(s): P38685, Q2M912

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents