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Protein

Putative acid--amine ligase YgiC

Gene

ygiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be a ligase forming an amide bond. Shows ATPase activity. Despite its similarity to the C-terminal synthetase domain of Gss, is not a glutathionylspermidine (Gsp) synthetase. Cannot synthesize Gsp, glutathione (GSH), or GSH intermediates, from GSH and spermidine, cysteine and glutamate, gamma-glutamylcysteine and spermidine, and gamma-glutamylcysteine and glycine. Does not bind to Gsp.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei100Transition state stabilizerBy similarity1
Metal bindingi102Magnesium 1By similarity1
Metal bindingi115Magnesium 1By similarity1
Metal bindingi115Magnesium 2By similarity1
Metal bindingi117Magnesium 2By similarity1
Binding sitei267ATPBy similarity1
Binding sitei302ATPBy similarity1
Binding sitei309ATP; via amide nitrogenBy similarity1
Binding sitei336ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 102ATPBy similarity3
Nucleotide bindingi371 – 373ATPBy similarity3

GO - Molecular functioni

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11165-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative acid--amine ligase YgiC (EC:6.3.1.-)
Gene namesi
Name:ygiC
Ordered Locus Names:b3038, JW3006
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11165 ygiC

Pathology & Biotechi

Disruption phenotypei

Deletion of this gene does not affect the ability to synthesize glutathionylspermidine. There is no visible differences between wild-type and mutant strain growth in LB media.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694011 – 386Putative acid--amine ligase YgiCAdd BLAST386

Proteomic databases

PaxDbiP0ADT5
PRIDEiP0ADT5

Interactioni

Protein-protein interaction databases

BioGridi4262389, 16 interactors
DIPiDIP-48176N
IntActiP0ADT5, 14 interactors
STRINGi316385.ECDH10B_3212

Structurei

3D structure databases

ProteinModelPortaliP0ADT5
SMRiP0ADT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106032 Bacteria
COG0754 LUCA
HOGENOMiHOG000249104
InParanoidiP0ADT5
OMAiQWNSLHE
PhylomeDBiP0ADT5

Family and domain databases

InterProiView protein in InterPro
IPR005494 GSPS_pre-ATP-grasp-like_dom
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF03738 GSP_synth, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit

Sequencei

Sequence statusi: Complete.

P0ADT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERVSITERP DWREKAHEYG FNFHTMYGEP YWCEDAYYKL TLAQVEKLEE
60 70 80 90 100
VTAELHQMCL KVVEKVIASD ELMTKFRIPK HTWSFVRQSW LTHQPSLYSR
110 120 130 140 150
LDLAWDGTGE PKLLENNADT PTSLYEAAFF QWIWLEDQLN AGNLPEGSDQ
160 170 180 190 200
FNSLQEKLID RFVELREQYG FQLLHLTCCR DTVEDRGTIQ YLQDCATEAE
210 220 230 240 250
IATEFLYIDD IGLGEKGQFT DLQDQVISNL FKLYPWEFML REMFSTKLED
260 270 280 290 300
AGVRWLEPAW KSIISNKALL PLLWEMFPNH PNLLPAYFAE DDHPQMEKYV
310 320 330 340 350
VKPIFSREGA NVSIIENGKT IEAAEGPYGE EGMIVQQFHP LPKFGDSYML
360 370 380
IGSWLVNDQP AGIGIREDRA LITQDMSRFY PHIFVE
Length:386
Mass (Da):45,026
Last modified:December 6, 2005 - v1
Checksum:i5DB19CE4F3FE4783
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77129 Genomic DNA Translation: AAA71876.1
U28377 Genomic DNA Translation: AAA69206.1
U00096 Genomic DNA Translation: AAC76074.1
AP009048 Genomic DNA Translation: BAE77094.1
PIRiS22361
RefSeqiNP_417510.1, NC_000913.3
WP_000442860.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76074; AAC76074; b3038
BAE77094; BAE77094; BAE77094
GeneIDi947249
KEGGiecj:JW3006
eco:b3038
PATRICifig|1411691.4.peg.3694

Similar proteinsi

Entry informationi

Entry nameiYGIC_ECOLI
AccessioniPrimary (citable) accession number: P0ADT5
Secondary accession number(s): P24196, Q2M9G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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