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Protein

Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase

Gene

ppnN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the N-glycosidic bond of diverse pyrimidine and purine nucleotide 5'-monophosphates, to form ribose 5-phosphate and the corresponding free base. Can use AMP, GMP, IMP, CMP, dTMP and UMP as substrates. Cannot catalyze the reverse reactions. Is required for optimal growth in glucose minimal medium, possibly because it contributes to nucleoside pool homeostasis by degrading excess nucleotides and feeding back the ribose moiety to catabolism.1 Publication

Catalytic activityi

A pyrimidine 5'-nucleotide + H2O = D-ribose 5-phosphate + a pyrimidine base.1 Publication
AMP + H2O = D-ribose 5-phosphate + adenine.1 Publication
GMP + H2O = D-ribose 5-phosphate + guanine.1 Publication
CMP + H2O = D-ribose 5-phosphate + cytosine.1 Publication
IMP + H2O = D-ribose 5-phosphate + hypoxanthine.1 Publication
UMP + H2O = D-ribose 5-phosphate + uracil.1 Publication
dTMP + H2O = 2-deoxy-D-ribose 5-phosphate + thymine.1 Publication

GO - Molecular functioni

  • AMP nucleosidase activity Source: UniProtKB-EC
  • pyrimidine-5'-nucleotide nucleosidase activity Source: EcoCyc

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG12373-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase1 Publication (EC:3.2.2.-1 Publication, EC:3.2.2.101 Publication)
Alternative name(s):
AMP nucleosidase1 Publication (EC:3.2.2.41 Publication)
CMP nucleosidase1 Publication
GMP nucleosidase1 Publication
IMP nucleosidase1 Publication
UMP nucleosidase1 Publication
dTMP nucleosidase1 Publication
Gene namesi
Name:ppnN1 Publication
Synonyms:ygdHImported
Ordered Locus Names:b2795, JW2766
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12373. ygdH.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a reduced growth rate and yield in glucose minimal medium compared to wild-type. They also show a consistent change in the level of several metabolites whose masses can correspond to adenine, xanthine or 4-hydroxy-L-threonine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001693341 – 454Pyrimidine/purine nucleotide 5'-monophosphate nucleosidaseAdd BLAST454

Proteomic databases

PaxDbiP0ADR8.
PRIDEiP0ADR8.

Interactioni

Protein-protein interaction databases

BioGridi4261305. 16 interactors.
DIPiDIP-47955N.
IntActiP0ADR8. 4 interactors.
MINTiMINT-1293384.
STRINGi316385.ECDH10B_2964.

Structurei

3D structure databases

ProteinModelPortaliP0ADR8.
SMRiP0ADR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LOG family.Curated

Phylogenomic databases

eggNOGiENOG4105EWT. Bacteria.
COG1611. LUCA.
HOGENOMiHOG000273722.
InParanoidiP0ADR8.
KOiK06966.

Family and domain databases

Gene3Di3.30.1850.10. 1 hit.
InterProiView protein in InterPro
IPR021826. DUF3412.
IPR031100. LOG_fam.
IPR037153. YgdH-like_sf.
IPR027820. YgdH_N.
PfamiView protein in Pfam
PF11892. DUF3412. 1 hit.
PF14793. DUF4478. 1 hit.
PF03641. Lysine_decarbox. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ADR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITHISPLGS MDMLSQLEVD MLKRTASSDL YQLFRNCSLA VLNSGSLTDN
60 70 80 90 100
SKELLSRFEN FDINVLRRER GVKLELINPP EEAFVDGRII RALQANLFAV
110 120 130 140 150
LRDILFVYGQ IHNTVRFPNL NLDNSVHITN LVFSILRNAR ALHVGEAPNM
160 170 180 190 200
VVCWGGHSIN ENEYLYARRV GNQLGLRELN ICTGCGPGAM EAPMKGAAVG
210 220 230 240 250
HAQQRYKDSR FIGMTEPSII AAEPPNPLVN ELIIMPDIEK RLEAFVRIAH
260 270 280 290 300
GIIIFPGGVG TAEELLYLLG ILMNPANKDQ VLPLILTGPK ESADYFRVLD
310 320 330 340 350
EFVVHTLGEN ARRHYRIIID DAAEVARQMK KSMPLVKENR RDTGDAYSFN
360 370 380 390 400
WSMRIAPDLQ MPFEPSHENM ANLKLYPDQP VEVLAADLRR AFSGIVAGNV
410 420 430 440 450
KEVGIRAIEE FGPYKINGDK EIMRRMDDLL QGFVAQHRMK LPGSAYIPCY

EICT
Length:454
Mass (Da):50,972
Last modified:December 6, 2005 - v1
Checksum:i39068DCC61DD1CF6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti389R → A in U01233 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29581 Genomic DNA. Translation: AAB40445.1.
U00096 Genomic DNA. Translation: AAC75837.1.
AP009048 Genomic DNA. Translation: BAE76867.1.
U01233 Genomic DNA. No translation available.
PIRiG65061.
RefSeqiNP_417275.1. NC_000913.3.
WP_000627995.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75837; AAC75837; b2795.
BAE76867; BAE76867; BAE76867.
GeneIDi947266.
KEGGiecj:JW2766.
eco:b2795.
PATRICifig|511145.12.peg.2895.

Similar proteinsi

Entry informationi

Entry nameiPPNN_ECOLI
AccessioniPrimary (citable) accession number: P0ADR8
Secondary accession number(s): P37350, Q2MA39, Q46921
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: October 25, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families