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Protein

Biotin transporter

Gene

bioP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Uptake of biotin. Acts probably by facilitated diffusion.1 Publication

Enzyme regulationi

Biotin uptake is weakly inhibited by CCCP and FCCP protonophores and by the respiratory chain blocker NaN3.1 Publication

Kineticsi

  1. KM=74 nM for biotin1 Publication

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11471-MONOMER
    MetaCyc:EG11471-MONOMER

    Protein family/group databases

    TCDBi2.A.7.3.61 the drug/metabolite transporter (dmt) superfamily

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin transporter1 Publication
    Alternative name(s):
    Plasmamembrane biotin transport protein1 Publication
    Gene namesi
    Name:bioP1 Publication
    Synonyms:yigM
    Ordered Locus Names:b3827, JW3803
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11471 yigM

    Subcellular locationi

    • Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei2 – 22HelicalSequence analysisAdd BLAST21
    Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
    Transmembranei56 – 76HelicalSequence analysisAdd BLAST21
    Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
    Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
    Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
    Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
    Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
    Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
    Transmembranei256 – 276HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Deletion of the gene abolishes biotin uptake (Ref. 5, PubMed:1091631). BioH-bioP double mutant is unable to grow on trace levels of biotin (PubMed:24256712).3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001696631 – 299Biotin transporterAdd BLAST299

    Proteomic databases

    PaxDbiP0ADP5
    PRIDEiP0ADP5

    Expressioni

    Inductioni

    Expression is repressed by biotin.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi4259475, 5 interactors
    STRINGi316407.85676224

    Structurei

    3D structure databases

    ProteinModelPortaliP0ADP5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini3 – 128EamA 1Sequence analysisAdd BLAST126
    Domaini139 – 274EamA 2Sequence analysisAdd BLAST136

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105EMK Bacteria
    COG0697 LUCA
    HOGENOMiHOG000269445
    InParanoidiP0ADP5
    OMAiRFNPWAL
    PhylomeDBiP0ADP5

    Family and domain databases

    InterProiView protein in InterPro
    IPR004779 CO/AA/NH_transpt
    IPR000620 EamA_dom
    PfamiView protein in Pfam
    PF00892 EamA, 2 hits
    TIGRFAMsiTIGR00950 2A78, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0ADP5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALLIITTIL WAFSFSFYGE YLAGHVDSYF AVLVRVGLAA LVFLPFLRTR
    60 70 80 90 100
    GNSLKTVGLY MLVGAMQLGV MYMLSFRAYL YLTVSELLLF TVLTPLYITL
    110 120 130 140 150
    IYDIMSKRRL RWGYAFSALL AVIGAGIIRY DQVTDHFWTG LLLVQLSNIT
    160 170 180 190 200
    FAIGMVGYKR LMETRPMPQH NAFAWFYLGA FLVAVIAWFL LGNAQKMPQT
    210 220 230 240 250
    TLQWGILVFL GVVASGIGYF MWNYGATQVD AGTLGIMNNM HVPAGLLVNL
    260 270 280 290
    AIWHQQPHWP TFITGALVIL ASLWVHRKWV APRSSQTADD RRRDCALSE
    Length:299
    Mass (Da):33,727
    Last modified:December 6, 2005 - v1
    Checksum:i2AF656D013405165
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M87049 Genomic DNA Translation: AAA67623.1
    U00096 Genomic DNA Translation: AAC76830.1
    AP009048 Genomic DNA Translation: BAE77474.1
    PIRiD65187
    RefSeqiNP_418271.1, NC_000913.3
    WP_001196238.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76830; AAC76830; b3827
    BAE77474; BAE77474; BAE77474
    GeneIDi948309
    KEGGiecj:JW3803
    eco:b3827
    PATRICifig|511145.12.peg.3943

    Similar proteinsi

    Entry informationi

    Entry nameiBIOP_ECOLI
    AccessioniPrimary (citable) accession number: P0ADP5
    Secondary accession number(s): P27849, Q2M8D2, Q8X5G7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: April 25, 2018
    This is version 88 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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