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Protein

Purine ribonucleoside efflux pump NepI

Gene

nepI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine.1 Publication

GO - Molecular functioni

  • purine nucleoside transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • purine nucleoside transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YICM-MONOMER.
ECOL316407:JW5938-MONOMER.
MetaCyc:YICM-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.26. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Purine ribonucleoside efflux pump NepI
Gene namesi
Name:nepI
Synonyms:yicM
Ordered Locus Names:b3662, JW5938
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11689. yicM.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Topological domaini43 – 54PeriplasmicSequence analysisAdd BLAST12
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 85CytoplasmicSequence analysis10
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Topological domaini107PeriplasmicSequence analysis1
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 147CytoplasmicSequence analysisAdd BLAST19
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 175PeriplasmicSequence analysis7
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 215CytoplasmicSequence analysisAdd BLAST19
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 255PeriplasmicSequence analysisAdd BLAST19
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 281CytoplasmicSequence analysis5
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Topological domaini303 – 305PeriplasmicSequence analysis3
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Topological domaini327 – 343CytoplasmicSequence analysisAdd BLAST17
Transmembranei344 – 364HelicalSequence analysisAdd BLAST21
Topological domaini365 – 366PeriplasmicSequence analysis2
Transmembranei367 – 387HelicalSequence analysisAdd BLAST21
Topological domaini388 – 396CytoplasmicSequence analysis9

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001696161 – 396Purine ribonucleoside efflux pump NepIAdd BLAST396

Proteomic databases

PaxDbiP0ADL1.
PRIDEiP0ADL1.

Expressioni

Inductioni

In stationary phase.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261472. 10 interactors.
DIPiDIP-48180N.
STRINGi511145.b3662.

Structurei

3D structure databases

ProteinModelPortaliP0ADL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RTG. Bacteria.
COG2814. LUCA.
HOGENOMiHOG000136928.
InParanoidiP0ADL1.
KOiK03445.
OMAiWTMATAT.
PhylomeDBiP0ADL1.

Family and domain databases

CDDicd06174. MFS. 1 hit.
HAMAPiMF_01189. MFS_NepI. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR023680. MFS_NepI.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ADL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFIAENRG ADAITRPNWS AVFSVAFCVA CLIIVEFLPV SLLTPMAQDL
60 70 80 90 100
GISEGVAGQS VTVTAFVAMF ASLFITQTIQ ATDRRYVVIL FAVLLTLSCL
110 120 130 140 150
LVSFANSFSL LLIGRACLGL ALGGFWAMSA SLTMRLVPPR TVPKALSVIF
160 170 180 190 200
GAVSIALVIA APLGSFLGEL IGWRNVFNAA AVMGVLCIFW IIKSLPSLPG
210 220 230 240 250
EPSHQKQNTF RLLQRPGVMA GMIAIFMSFA GQFAFFTYIR PVYMNLAGFG
260 270 280 290 300
VDGLTLVLLS FGIASFIGTS LSSFILKRSV KLALAGAPLI LAVSALVLTL
310 320 330 340 350
WGSDKIVATG VAIIWGLTFA LVPVGWSTWI TRSLADQAEK AGSIQVAVIQ
360 370 380 390
LANTCGAAIG GYALDNIGLT SPLMLSGTLM LLTALLVTAK VKMKKS
Length:396
Mass (Da):41,842
Last modified:July 10, 2007 - v2
Checksum:iDB240A4A8C1B9D1B
GO

Sequence cautioni

The sequence AAA62014 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAW51767 differs from that shown. Reason: Frameshift at position 367.Curated
The sequence BAE77631 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti158V → M in strain: BEN2908 / O2:K1:H5 / APEC. 1
Natural varianti182V → A in strain: BEN2908 / O2:K1:H5 / APEC. 1
Natural varianti267I → V in strain: BEN2908 / O2:K1:H5 / APEC. 1
Natural varianti289 – 290LI → FV in strain: BEN2908 / O2:K1:H5 / APEC. 2
Natural varianti293V → L in strain: BEN2908 / O2:K1:H5 / APEC. 1
Natural varianti322V → I in strain: BEN2908 / O2:K1:H5 / APEC. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62014.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76685.2.
AP009048 Genomic DNA. Translation: BAE77631.1. Different initiation.
AY857617 Genomic DNA. Translation: AAW51767.1. Frameshift.
PIRiG65167.
RefSeqiNP_418118.2. NC_000913.3.
WP_001288549.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76685; AAC76685; b3662.
BAE77631; BAE77631; BAE77631.
GeneIDi948213.
KEGGiecj:JW5938.
eco:b3662.
PATRICi32122817. VBIEscCol129921_3784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62014.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76685.2.
AP009048 Genomic DNA. Translation: BAE77631.1. Different initiation.
AY857617 Genomic DNA. Translation: AAW51767.1. Frameshift.
PIRiG65167.
RefSeqiNP_418118.2. NC_000913.3.
WP_001288549.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ADL1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261472. 10 interactors.
DIPiDIP-48180N.
STRINGi511145.b3662.

Protein family/group databases

TCDBi2.A.1.2.26. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP0ADL1.
PRIDEiP0ADL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76685; AAC76685; b3662.
BAE77631; BAE77631; BAE77631.
GeneIDi948213.
KEGGiecj:JW5938.
eco:b3662.
PATRICi32122817. VBIEscCol129921_3784.

Organism-specific databases

EchoBASEiEB1640.
EcoGeneiEG11689. yicM.

Phylogenomic databases

eggNOGiENOG4107RTG. Bacteria.
COG2814. LUCA.
HOGENOMiHOG000136928.
InParanoidiP0ADL1.
KOiK03445.
OMAiWTMATAT.
PhylomeDBiP0ADL1.

Enzyme and pathway databases

BioCyciEcoCyc:YICM-MONOMER.
ECOL316407:JW5938-MONOMER.
MetaCyc:YICM-MONOMER.

Miscellaneous databases

PROiP0ADL1.

Family and domain databases

CDDicd06174. MFS. 1 hit.
HAMAPiMF_01189. MFS_NepI. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR023680. MFS_NepI.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEPI_ECOLI
AccessioniPrimary (citable) accession number: P0ADL1
Secondary accession number(s): P31438
, P76725, Q2M7X5, Q5I3I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.