Isochorismatase - Escherichia coli (strain K12)

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P0ADI4 (ENTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Isochorismatase
EC=3.3.2.1

Alternative name(s):
2,3 dihydro-2,3 dihydroxybenzoate synthase
Enterobactin synthase component B
Enterochelin synthase B
Isochorismate lyase

Gene names

Name:	entB
Synonyms:	entG
Ordered Locus Names:	b0595, JW0587

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly.
Catalytic activity	Isochorismate + H₂O = 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.
Pathway	Siderophore biosynthesis; enterobactin biosynthesis.
Subunit structure	Proteins EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.
Post-translational modification	4'-phosphopantetheine is transferred from CoA to a specific serine of apo-EntB by EntD. Holo-EntB so formed is then acylated with 2,3-dihydroxybenzoate in a reaction catalyzed by EntE.
Sequence similarities	Belongs to the isochorismatase family. Contains 1 acyl carrier domain.

Ontologies

Keywords
Biological process	Enterobactin biosynthesis
Ligand	Phosphopantetheine
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	enterobactin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	isochorismatase activity Inferred from electronic annotation. Source: EC transferase activity, transferring alkyl or aryl (other than methyl) groups Inferred from mutant phenotype Ref.6. Source: EcoliWiki
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
entH	P0A8Y8	3	EBI-547993,EBI-1118982

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Isochorismatase

PRO_0000201822

Regions

Domain

214 – 285

Acyl carrier

Region

26 – 208

183

Isochorismate lyase

Amino acid modifications

Modified residue

245

O-(pantetheine 4'-phosphoryl)serine Potential

Secondary structure

285

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ADI4 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: E98C62D5ED23BAB1
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FASTA

285

32,554

        10         20         30         40         50         60 
MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC PMMEQVIANI 

        70         80         90        100        110        120 
AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQKVVD RLTPDADDTV 

       130        140        150        160        170        180 
LVKWRYSAFH RSPLEQMLKE SGRNQLIITG VYAHIGCMTT ATDAFMRDIK PFMVADALAD 

       190        200        210        220        230        240 
FSRDEHLMSL KYVAGRSGRV VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID 

       250        260        270        280 
YGLDSVRMMA LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase." Liu J., Duncan K., Walsh C.T. J. Bacteriol. 171:791-798(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA." Nahlik M.S., Brickman T.J., Ozenberger B.A., McIntosh M.A. J. Bacteriol. 171:784-790(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate." Gehring A.M., Bradley K.A., Walsh C.T. Biochemistry 36:8495-8503(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M24148 Unassigned DNA. Translation: AAA16102.1.
M24143 Genomic DNA. Translation: AAA76835.1.
U82598 Genomic DNA. Translation: AAB40795.1.
U00096 Genomic DNA. Translation: AAC73696.1.
AP009048 Genomic DNA. Translation: BAE76350.1.

PIR

YXECIC. C91904.

RefSeq

NP_415127.1. NC_000913.2.
YP_488884.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FQ1	X-ray	2.30	A/B	1-285	[»]
3RG2	X-ray	3.10	A/B/C/D/E/F/G/H/I/J	211-285	[»]

ProteinModelPortal

P0ADI4.

SMR

P0ADI4. Positions 4-282.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0ADI4. 24 interactions.

MINT

MINT-1241343.

STRING

511145.b0595.

2D gel databases

SWISS-2DPAGE

P0ADI4.

Proteomic databases

PaxDb

P0ADI4.

PRIDE

P0ADI4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73696; AAC73696; b0595.
BAE76350; BAE76350; BAE76350.

GeneID

12931911.
946178.

KEGG

ecj:Y75_p0584.
eco:b0595.

PATRIC

32116366. VBIEscCol129921_0623.

Organism-specific databases

EchoBASE

EB0256.

EcoGene

EG10260. entB.

Phylogenomic databases

eggNOG

COG3433.

HOGENOM

HOG000078667.

K01252.

OMA

MWGPGLN.

ProtClustDB

CLSK879705.

Enzyme and pathway databases

BioCyc

EcoCyc:ENTB-MONOMER.
ECOL316407:JW0587-MONOMER.
MetaCyc:ENTB-MONOMER.

SABIO-RK

P0ADI4.

UniPathway

UPA00017.

Gene expression databases

Genevestigator

P0ADI4.

Family and domain databases

Gene3D

1.10.1200.10. 1 hit.
3.40.50.850. 1 hit.

InterPro

IPR009081. Acyl_carrier_prot-like.
IPR016291. Isochorismatase.
IPR000868. Isochorismatase-like.
[Graphical view]

Pfam

PF00857. Isochorismatase. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]

PIRSF

PIRSF001111. Isochorismatase. 1 hit.

PRINTS

PR01398. ISCHRISMTASE.

SUPFAM

SSF52499. Iscrsm_hydrolase. 1 hit.

PROSITE

PS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0ADI4.

Entry information

Entry name

ENTB_ECOLI

Accession

Primary (citable) accession number: P0ADI4
Secondary accession number(s): P15048, Q2MBK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents