Reviewed, UniProtKB/Swiss-Prot P0ADH7 (FIME_ECOLI)

Names and origin

Protein names

Recommended name:
    Type 1 fimbriae regulatory protein fimE

Gene names

Name:	fimE
Ordered Locus Names:	b4313, JW4276

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	FimE is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, fimA. FimE switches fimA off.
Sequence similarities	Belongs to the 'phage' integrase family.

Ontologies

Keywords
Biological process	DNA integration DNA recombination Fimbrium biogenesis Transcription Transcription regulation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Type 1 fimbriae regulatory protein fimE		PRO_0000197541
Sites
	Active site	171	1	O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ADH7-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: A33139B0378CB37D	FASTA	198	23,116
	10 20 30 40 50 60 MSKRRYLTGK EVQAMMQAVC YGATGARDYC LILLAYRHGM RISELLDLHY QDLDLNEGRI 70 80 90 100 110 120 NIRRLKNGFS TVHPLRFDER EAVERWTQER ANWKGADRTD AIFISRRGSR LSRQQAYRII 130 140 150 160 170 180 RDAGIEAGTV TQTHPHMLRH ACGYELAERG ADTRLIQDYL GHRNIRHTVR YTASNAARFA 190 GLWERNNLIN EKLKREEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli." Klemm P. EMBO J. 5:1389-1393(1986) [PubMed: 2874022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03923 Genomic DNA. Translation: CAA27561.1. U14003 Genomic DNA. Translation: AAA97209.1. U00096 Genomic DNA. Translation: AAC77269.1. AP009048 Genomic DNA. Translation: BAE78306.1.
PIR	RGECFE. B25111.
RefSeq	AP_004805.1. NP_418733.1.
3D structure databases
SMR	P0ADH7. Positions 3-174.
ModBase	Search...
Protein-protein interaction databases
STRING	P0ADH7.
Genome annotation databases
GeneID	948836.
GenomeReviews	Gene locus JW4276 in contig AP009048_GR. Gene locus b4313 in contig U00096_GR.
KEGG	ecj:JW4276. eco:b4313.
Organism-specific databases
EchoBASE	EB0308.
EcoGene	EG10312. fimE.
CMR	Search...
Phylogenomic databases
eggNOG	COG0582.
HOGENOM	HBG287305.
OMA	TDALFIS.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10312-MONOMER. ECOL168927:B4313-MONOMER.
Gene expression databases
Genevestigator	P0ADH7.
Family and domain databases
InterPro	IPR011010. DNA_brk_join_enz. IPR013762. Integrase-like_cat-core_phage. IPR002104. Integrase_cat-core_phage. [Graphical view]
Gene3D	G3DSA:1.10.443.10. Phage_intgr_like. 1 hit.
Pfam	PF00589. Phage_integrase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIME_ECOLI

Accession

Primary (citable) accession number: P0ADH7
Secondary accession number(s): P04741, Q2M600

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)