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Protein

Type 1 fimbriae regulatory protein FimB

Gene

fimB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, FimA. FimB switches FimA on.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei176 – 1761O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA integration Source: EcoCyc
  • DNA recombination Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination, Fimbrium biogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10309-MONOMER.
ECOL316407:JW4275-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 1 fimbriae regulatory protein FimB
Gene namesi
Name:fimB
Ordered Locus Names:b4312, JW4275
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10309. fimB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Type 1 fimbriae regulatory protein FimBPRO_0000197539Add
BLAST

Proteomic databases

PaxDbiP0ADH5.

Interactioni

Protein-protein interaction databases

BioGridi4259374. 158 interactions.
DIPiDIP-47842N.
IntActiP0ADH5. 23 interactions.
MINTiMINT-1221137.
STRINGi511145.b4312.

Structurei

3D structure databases

ProteinModelPortaliP0ADH5.
SMRiP0ADH5. Positions 12-174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

eggNOGiENOG4108W9T. Bacteria.
ENOG410YF4U. LUCA.
HOGENOMiHOG000117333.
InParanoidiP0ADH5.
KOiK07357.
OMAiNFLTQNE.
OrthoDBiEOG6PZXFP.
PhylomeDBiP0ADH5.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
[Graphical view]
PfamiPF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ADH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNKADNKKR NFLTHSEIES LLKAANTGPH AARNYCLTLL CFIHGFRASE
60 70 80 90 100
ICRLRISDID LKAKCIYIHR LKKGFSTTHP LLNKEVQALK NWLSIRTSYP
110 120 130 140 150
HAESEWVFLS RKGNPLSRQQ FYHIISTSGG NAGLSLEIHP HMLRHSCGFA
160 170 180 190 200
LANMGIDTRL IQDYLGHRNI RHTVWYTASN AGRFYGIWDR ARGRQRHAVL
Length:200
Mass (Da):22,993
Last modified:December 6, 2005 - v1
Checksum:i36BFC656C68837C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131L → V in AAA23779 (PubMed:8121417).Curated
Sequence conflicti32 – 321A → G in AAA23779 (PubMed:8121417).Curated
Sequence conflicti85 – 851E → D in CAA27560 (PubMed:2874022).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03923 Genomic DNA. Translation: CAA27560.1.
U14003 Genomic DNA. Translation: AAA97208.1.
U00096 Genomic DNA. Translation: AAC77268.1.
AP009048 Genomic DNA. Translation: BAE78305.1.
L07756 Genomic DNA. Translation: AAA23779.1.
PIRiS56537. RGECFF.
RefSeqiNP_418732.1. NC_000913.3.
WP_000790583.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77268; AAC77268; b4312.
BAE78305; BAE78305; BAE78305.
GeneIDi948832.
KEGGiecj:JW4275.
eco:b4312.
PATRICi32124220. VBIEscCol129921_4453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03923 Genomic DNA. Translation: CAA27560.1.
U14003 Genomic DNA. Translation: AAA97208.1.
U00096 Genomic DNA. Translation: AAC77268.1.
AP009048 Genomic DNA. Translation: BAE78305.1.
L07756 Genomic DNA. Translation: AAA23779.1.
PIRiS56537. RGECFF.
RefSeqiNP_418732.1. NC_000913.3.
WP_000790583.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ADH5.
SMRiP0ADH5. Positions 12-174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259374. 158 interactions.
DIPiDIP-47842N.
IntActiP0ADH5. 23 interactions.
MINTiMINT-1221137.
STRINGi511145.b4312.

Proteomic databases

PaxDbiP0ADH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77268; AAC77268; b4312.
BAE78305; BAE78305; BAE78305.
GeneIDi948832.
KEGGiecj:JW4275.
eco:b4312.
PATRICi32124220. VBIEscCol129921_4453.

Organism-specific databases

EchoBASEiEB0305.
EcoGeneiEG10309. fimB.

Phylogenomic databases

eggNOGiENOG4108W9T. Bacteria.
ENOG410YF4U. LUCA.
HOGENOMiHOG000117333.
InParanoidiP0ADH5.
KOiK07357.
OMAiNFLTQNE.
OrthoDBiEOG6PZXFP.
PhylomeDBiP0ADH5.

Enzyme and pathway databases

BioCyciEcoCyc:EG10309-MONOMER.
ECOL316407:JW4275-MONOMER.

Miscellaneous databases

PROiP0ADH5.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
[Graphical view]
PfamiPF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli."
    Klemm P.
    EMBO J. 5:1389-1393(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Analysis of the fimB promoter region involved in type 1 pilus phase variation in Escherichia coli."
    Schwan W.R., Seifert H.S., Duncan J.L.
    Mol. Gen. Genet. 242:623-630(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
    Strain: ATCC 700336 / J96.

Entry informationi

Entry nameiFIMB_ECOLI
AccessioniPrimary (citable) accession number: P0ADH5
Secondary accession number(s): P04742, Q2M601, Q47219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 6, 2005
Last modified: April 13, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.