Reviewed, UniProtKB/Swiss-Prot P0ADG6 (SUHB_ECO57)

Names and origin

Protein names

Recommended name:
    Inositol-1-monophosphatase
      Short name=IMPase
      Short name=Inositol-1-phosphatase
      Short name=I-1-Pase
    EC=3.1.3.25

Gene names

Name:	suhB
Ordered Locus Names:	Z3800, ECs3399

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	inositol-1(or 4)-monophosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Inositol-1-monophosphatase		PRO_0000142560
Regions
	Region	86 – 89	4	Substrate binding By similarity
Sites
	Metal binding	67	1	Magnesium 1 By similarity
	Metal binding	84	1	Magnesium 1 By similarity
	Metal binding	84	1	Magnesium 2 By similarity
	Metal binding	86	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	87	1	Magnesium 2 By similarity
	Metal binding	212	1	Magnesium 2 By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ADG6-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 8FEC3508BD111301	FASTA	267	29,172
	10 20 30 40 50 60 MHPMLNIAVR AARKAGNLIA KNYETPDAVE ASQKGSNDFV TNVDKAAEAV IIDTIRKSYP 70 80 90 100 110 120 QHTIITEESG ELEGTDQDVQ WVIDPLDGTT NFIKRLPHFA VSIAVRIKGR TEVAVVYDPM 130 140 150 160 170 180 RNELFTATRG QGAQLNGYRL RGSTARDLDG TILATGFPFK AKQYATTYIN IVGKLFNECA 190 200 210 220 230 240 DFRRTGSAAL DLAYVAAGRV DGFFEIGLRP WDFAAGELLV REAGGIVSDF TGGHNYMLTG 250 260 NIVAGNPRVV KAMLANMRDE LSDALKR

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. No translation available. BA000007 Genomic DNA. Translation: BAB36822.1.
PIR	G91053.
RefSeq	NP_311426.1.
3D structure databases
HSSP	HSSP built from PDB template 1IMF based on UniProtKB P29218.
ModBase	Search...
Genome annotation databases
GeneID	915157.
GenomeReviews	Gene locus ECs3399 in contig AE005174_GR. Gene locus ECs3399 in contig BA000007_GR.
KEGG	ecs:ECs3399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0ADG6.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3399-MON.
Family and domain databases
InterPro	IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES.
ProDom	PD023420. Inositol_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUHB_ECO57

Accession

Primary (citable) accession number: P0ADG6
Secondary accession number(s): P22783, P77511, Q8X2E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)