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P0ADG1

- ILVM_ECOLI

UniProt

P0ADG1 - ILVM_ECOLI

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Protein

Acetolactate synthase isozyme 2 small subunit

Gene

ilvM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Binds 1 magnesium ion per subunit.By similarity
Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi

GO - Molecular functioni

  1. acetolactate synthase activity Source: EcoCyc
  2. amino acid binding Source: InterPro

GO - Biological processi

  1. cellular amino acid biosynthetic process Source: EcoCyc
  2. isoleucine biosynthetic process Source: UniProtKB-UniPathway
  3. valine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Magnesium, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciEcoCyc:SMALLILVM-MONOMER.
ECOL316407:JW3742-MONOMER.
MetaCyc:SMALLILVM-MONOMER.
SABIO-RKP0ADG1.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 2 small subunit (EC:2.2.1.6)
Alternative name(s):
ALS-II
Acetohydroxy-acid synthase II small subunit
Short name:
AHAS-II
Gene namesi
Name:ilvM
Ordered Locus Names:b3769, JW3742
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10501. ilvM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8787Acetolactate synthase isozyme 2 small subunitPRO_0000151429Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0ADG1.

Interactioni

Subunit structurei

Tetramer of two large and two small chains.

Protein-protein interaction databases

IntActiP0ADG1. 3 interactions.
STRINGi511145.b3769.

Structurei

3D structure databases

ProteinModelPortaliP0ADG1.
SMRiP0ADG1. Positions 2-82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7874ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3978.
HOGENOMiHOG000286146.
KOiK11258.
OMAiTTQQIRA.
OrthoDBiEOG69GZRD.

Family and domain databases

InterProiIPR002912. ACT_dom.
[Graphical view]
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ADG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMQHQVNVSA RFNPETLERV LRVVRHRGFH VCSMNMAAAS DAQNINIELT
60 70 80
VASPRSVDLL FSQLNKLVDV AHVAICQSTT TSQQIRA
Length:87
Mass (Da):9,703
Last modified:December 4, 2007 - v2
Checksum:iCEB6B5211262E0AF
GO

Sequence cautioni

The sequence AAB59051.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA26261.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02413 Genomic DNA. Translation: CAA26261.1. Different initiation.
M10313 Genomic DNA. Translation: AAB59051.1. Different initiation.
X04890 Genomic DNA. Translation: CAA28574.1.
M87049 Genomic DNA. Translation: AAA67572.1.
U00096 Genomic DNA. Translation: AAC77489.1.
AP009048 Genomic DNA. Translation: BAE77528.1.
RefSeqiNP_418217.1. NC_000913.3.
YP_491669.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77489; AAC77489; b3769.
BAE77528; BAE77528; BAE77528.
GeneIDi12934298.
948279.
KEGGiecj:Y75_p3406.
eco:b3769.
PATRICi32123033. VBIEscCol129921_3886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02413 Genomic DNA. Translation: CAA26261.1 . Different initiation.
M10313 Genomic DNA. Translation: AAB59051.1 . Different initiation.
X04890 Genomic DNA. Translation: CAA28574.1 .
M87049 Genomic DNA. Translation: AAA67572.1 .
U00096 Genomic DNA. Translation: AAC77489.1 .
AP009048 Genomic DNA. Translation: BAE77528.1 .
RefSeqi NP_418217.1. NC_000913.3.
YP_491669.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0ADG1.
SMRi P0ADG1. Positions 2-82.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0ADG1. 3 interactions.
STRINGi 511145.b3769.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77489 ; AAC77489 ; b3769 .
BAE77528 ; BAE77528 ; BAE77528 .
GeneIDi 12934298.
948279.
KEGGi ecj:Y75_p3406.
eco:b3769.
PATRICi 32123033. VBIEscCol129921_3886.

Organism-specific databases

EchoBASEi EB0496.
EcoGenei EG10501. ilvM.

Phylogenomic databases

eggNOGi COG3978.
HOGENOMi HOG000286146.
KOi K11258.
OMAi TTQQIRA.
OrthoDBi EOG69GZRD.

Enzyme and pathway databases

UniPathwayi UPA00047 ; UER00055 .
UPA00049 ; UER00059 .
BioCyci EcoCyc:SMALLILVM-MONOMER.
ECOL316407:JW3742-MONOMER.
MetaCyc:SMALLILVM-MONOMER.
SABIO-RK P0ADG1.

Miscellaneous databases

PROi P0ADG1.

Gene expression databases

Genevestigatori P0ADG1.

Family and domain databases

InterProi IPR002912. ACT_dom.
[Graphical view ]
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence."
    Kuramitsu S., Ogawa T., Ogawa H., Kagamiyama H.
    J. Biochem. 97:993-999(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12."
    Lawther R.P., Wek R.C., Lopes J.M., Pereira R., Taillon B.E., Hatfield G.W.
    Nucleic Acids Res. 15:2137-2155(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: K12.
  3. "DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli."
    Pagel J.M., Winkelman J.W., Adams C.W., Hatfield G.W.
    J. Mol. Biol. 224:919-935(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  4. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiILVM_ECOLI
AccessioniPrimary (citable) accession number: P0ADG1
Secondary accession number(s): P13048, P78269, Q2M878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 4, 2007
Last modified: October 1, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3