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P0ADG1 (ILVM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetolactate synthase isozyme 2 small subunit

EC=2.2.1.6
Alternative name(s):
ALS-II
Acetohydroxy-acid synthase II small subunit
Short name=AHAS-II
Gene names
Name:ilvM
Ordered Locus Names:b3769, JW3742
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length87 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2 pyruvate = 2-acetolactate + CO2.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.

Subunit structure

Tetramer of two large and two small chains.

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Sequence similarities

Contains 1 ACT domain.

Sequence caution

The sequence AAB59051.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA26261.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8787Acetolactate synthase isozyme 2 small subunit
PRO_0000151429

Regions

Domain5 – 7874ACT

Sequences

Sequence LengthMass (Da)Tools
P0ADG1 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: CEB6B5211262E0AF

FASTA879,703
        10         20         30         40         50         60 
MMQHQVNVSA RFNPETLERV LRVVRHRGFH VCSMNMAAAS DAQNINIELT VASPRSVDLL 

        70         80 
FSQLNKLVDV AHVAICQSTT TSQQIRA 

« Hide

References

« Hide 'large scale' references
[1]"Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence."
Kuramitsu S., Ogawa T., Ogawa H., Kagamiyama H.
J. Biochem. 97:993-999(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12."
Lawther R.P., Wek R.C., Lopes J.M., Pereira R., Taillon B.E., Hatfield G.W.
Nucleic Acids Res. 15:2137-2155(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: K12.
[3]"DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli."
Pagel J.M., Winkelman J.W., Adams C.W., Hatfield G.W.
J. Mol. Biol. 224:919-935(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[4]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02413 Genomic DNA. Translation: CAA26261.1. Different initiation.
M10313 Genomic DNA. Translation: AAB59051.1. Different initiation.
X04890 Genomic DNA. Translation: CAA28574.1.
M87049 Genomic DNA. Translation: AAA67572.1.
U00096 Genomic DNA. Translation: AAC77489.1.
AP009048 Genomic DNA. Translation: BAE77528.1.
RefSeqNP_418217.1. NC_000913.3.
YP_491669.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0ADG1.
SMRP0ADG1. Positions 2-82.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0ADG1. 3 interactions.
STRING511145.b3769.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77489; AAC77489; b3769.
BAE77528; BAE77528; BAE77528.
GeneID12934298.
948279.
KEGGecj:Y75_p3406.
eco:b3769.
PATRIC32123033. VBIEscCol129921_3886.

Organism-specific databases

EchoBASEEB0496.
EcoGeneEG10501. ilvM.

Phylogenomic databases

eggNOGCOG3978.
HOGENOMHOG000286146.
KOK11258.
OMATTQQIRA.
OrthoDBEOG69GZRD.
ProtClustDBPRK11152.

Enzyme and pathway databases

BioCycEcoCyc:SMALLILVM-MONOMER.
ECOL316407:JW3742-MONOMER.
MetaCyc:SMALLILVM-MONOMER.
SABIO-RKP0ADG1.
UniPathwayUPA00047; UER00055.
UPA00049; UER00059.

Gene expression databases

GenevestigatorP0ADG1.

Family and domain databases

InterProIPR002912. ACT_dom.
[Graphical view]
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0ADG1.

Entry information

Entry nameILVM_ECOLI
AccessionPrimary (citable) accession number: P0ADG1
Secondary accession number(s): P13048, P78269, Q2M878
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene