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P0ADG1

- ILVM_ECOLI

UniProt

P0ADG1 - ILVM_ECOLI

Protein

Acetolactate synthase isozyme 2 small subunit

Gene

ilvM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    2 pyruvate = 2-acetolactate + CO2.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 thiamine pyrophosphate per subunit.By similarity

    Pathwayi

    GO - Molecular functioni

    1. acetolactate synthase activity Source: EcoCyc
    2. amino acid binding Source: InterPro

    GO - Biological processi

    1. cellular amino acid biosynthetic process Source: EcoCyc
    2. isoleucine biosynthetic process Source: UniProtKB-UniPathway
    3. valine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

    Keywords - Ligandi

    Magnesium, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:SMALLILVM-MONOMER.
    ECOL316407:JW3742-MONOMER.
    MetaCyc:SMALLILVM-MONOMER.
    SABIO-RKP0ADG1.
    UniPathwayiUPA00047; UER00055.
    UPA00049; UER00059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetolactate synthase isozyme 2 small subunit (EC:2.2.1.6)
    Alternative name(s):
    ALS-II
    Acetohydroxy-acid synthase II small subunit
    Short name:
    AHAS-II
    Gene namesi
    Name:ilvM
    Ordered Locus Names:b3769, JW3742
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10501. ilvM.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8787Acetolactate synthase isozyme 2 small subunitPRO_0000151429Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0ADG1.

    Interactioni

    Subunit structurei

    Tetramer of two large and two small chains.

    Protein-protein interaction databases

    IntActiP0ADG1. 3 interactions.
    STRINGi511145.b3769.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ADG1.
    SMRiP0ADG1. Positions 2-82.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7874ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ACT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3978.
    HOGENOMiHOG000286146.
    KOiK11258.
    OMAiTTQQIRA.
    OrthoDBiEOG69GZRD.

    Family and domain databases

    InterProiIPR002912. ACT_dom.
    [Graphical view]
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ADG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMQHQVNVSA RFNPETLERV LRVVRHRGFH VCSMNMAAAS DAQNINIELT   50
    VASPRSVDLL FSQLNKLVDV AHVAICQSTT TSQQIRA 87
    Length:87
    Mass (Da):9,703
    Last modified:December 4, 2007 - v2
    Checksum:iCEB6B5211262E0AF
    GO

    Sequence cautioni

    The sequence AAB59051.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA26261.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02413 Genomic DNA. Translation: CAA26261.1. Different initiation.
    M10313 Genomic DNA. Translation: AAB59051.1. Different initiation.
    X04890 Genomic DNA. Translation: CAA28574.1.
    M87049 Genomic DNA. Translation: AAA67572.1.
    U00096 Genomic DNA. Translation: AAC77489.1.
    AP009048 Genomic DNA. Translation: BAE77528.1.
    RefSeqiNP_418217.1. NC_000913.3.
    YP_491669.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77489; AAC77489; b3769.
    BAE77528; BAE77528; BAE77528.
    GeneIDi12934298.
    948279.
    KEGGiecj:Y75_p3406.
    eco:b3769.
    PATRICi32123033. VBIEscCol129921_3886.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02413 Genomic DNA. Translation: CAA26261.1 . Different initiation.
    M10313 Genomic DNA. Translation: AAB59051.1 . Different initiation.
    X04890 Genomic DNA. Translation: CAA28574.1 .
    M87049 Genomic DNA. Translation: AAA67572.1 .
    U00096 Genomic DNA. Translation: AAC77489.1 .
    AP009048 Genomic DNA. Translation: BAE77528.1 .
    RefSeqi NP_418217.1. NC_000913.3.
    YP_491669.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0ADG1.
    SMRi P0ADG1. Positions 2-82.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0ADG1. 3 interactions.
    STRINGi 511145.b3769.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77489 ; AAC77489 ; b3769 .
    BAE77528 ; BAE77528 ; BAE77528 .
    GeneIDi 12934298.
    948279.
    KEGGi ecj:Y75_p3406.
    eco:b3769.
    PATRICi 32123033. VBIEscCol129921_3886.

    Organism-specific databases

    EchoBASEi EB0496.
    EcoGenei EG10501. ilvM.

    Phylogenomic databases

    eggNOGi COG3978.
    HOGENOMi HOG000286146.
    KOi K11258.
    OMAi TTQQIRA.
    OrthoDBi EOG69GZRD.

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00055 .
    UPA00049 ; UER00059 .
    BioCyci EcoCyc:SMALLILVM-MONOMER.
    ECOL316407:JW3742-MONOMER.
    MetaCyc:SMALLILVM-MONOMER.
    SABIO-RK P0ADG1.

    Miscellaneous databases

    PROi P0ADG1.

    Gene expression databases

    Genevestigatori P0ADG1.

    Family and domain databases

    InterProi IPR002912. ACT_dom.
    [Graphical view ]
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence."
      Kuramitsu S., Ogawa T., Ogawa H., Kagamiyama H.
      J. Biochem. 97:993-999(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12."
      Lawther R.P., Wek R.C., Lopes J.M., Pereira R., Taillon B.E., Hatfield G.W.
      Nucleic Acids Res. 15:2137-2155(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: K12.
    3. "DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli."
      Pagel J.M., Winkelman J.W., Adams C.W., Hatfield G.W.
      J. Mol. Biol. 224:919-935(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    4. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiILVM_ECOLI
    AccessioniPrimary (citable) accession number: P0ADG1
    Secondary accession number(s): P13048, P78269, Q2M878
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3