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Protein

Acetolactate synthase isozyme 2 small subunit

Gene

ilvM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase isozyme 2 large subunit (ilvG)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase isozyme 2 large subunit (ilvG)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • acetolactate synthase activity Source: EcoCyc
  • amino acid binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Magnesium, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciEcoCyc:SMALLILVM-MONOMER.
ECOL316407:JW3742-MONOMER.
MetaCyc:SMALLILVM-MONOMER.
SABIO-RKP0ADG1.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 2 small subunit (EC:2.2.1.6)
Alternative name(s):
ALS-II
Acetohydroxy-acid synthase II small subunit
Short name:
AHAS-II
Gene namesi
Name:ilvM
Ordered Locus Names:b3769, JW3742
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10501. ilvM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514291 – 87Acetolactate synthase isozyme 2 small subunitAdd BLAST87

Proteomic databases

PaxDbiP0ADG1.
PRIDEiP0ADG1.

Interactioni

Subunit structurei

Tetramer of two large and two small chains.

Protein-protein interaction databases

BioGridi4263292. 7 interactors.
IntActiP0ADG1. 3 interactors.
STRINGi511145.b3769.

Structurei

3D structure databases

ProteinModelPortaliP0ADG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 78ACTPROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105P3Y. Bacteria.
COG3978. LUCA.
HOGENOMiHOG000286146.
KOiK11258.
OMAiTRHRGFQ.

Family and domain databases

InterProiIPR002912. ACT_dom.
[Graphical view]
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ADG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQHQVNVSA RFNPETLERV LRVVRHRGFH VCSMNMAAAS DAQNINIELT
60 70 80
VASPRSVDLL FSQLNKLVDV AHVAICQSTT TSQQIRA
Length:87
Mass (Da):9,703
Last modified:December 4, 2007 - v2
Checksum:iCEB6B5211262E0AF
GO

Sequence cautioni

The sequence AAB59051 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA26261 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02413 Genomic DNA. Translation: CAA26261.1. Different initiation.
M10313 Genomic DNA. Translation: AAB59051.1. Different initiation.
X04890 Genomic DNA. Translation: CAA28574.1.
M87049 Genomic DNA. Translation: AAA67572.1.
U00096 Genomic DNA. Translation: AAC77489.1.
AP009048 Genomic DNA. Translation: BAE77528.1.
RefSeqiNP_418217.1. NC_000913.3.
WP_000983255.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77489; AAC77489; b3769.
BAE77528; BAE77528; BAE77528.
GeneIDi948279.
KEGGiecj:JW3742.
eco:b3769.
PATRICi32123033. VBIEscCol129921_3886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02413 Genomic DNA. Translation: CAA26261.1. Different initiation.
M10313 Genomic DNA. Translation: AAB59051.1. Different initiation.
X04890 Genomic DNA. Translation: CAA28574.1.
M87049 Genomic DNA. Translation: AAA67572.1.
U00096 Genomic DNA. Translation: AAC77489.1.
AP009048 Genomic DNA. Translation: BAE77528.1.
RefSeqiNP_418217.1. NC_000913.3.
WP_000983255.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ADG1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263292. 7 interactors.
IntActiP0ADG1. 3 interactors.
STRINGi511145.b3769.

Proteomic databases

PaxDbiP0ADG1.
PRIDEiP0ADG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77489; AAC77489; b3769.
BAE77528; BAE77528; BAE77528.
GeneIDi948279.
KEGGiecj:JW3742.
eco:b3769.
PATRICi32123033. VBIEscCol129921_3886.

Organism-specific databases

EchoBASEiEB0496.
EcoGeneiEG10501. ilvM.

Phylogenomic databases

eggNOGiENOG4105P3Y. Bacteria.
COG3978. LUCA.
HOGENOMiHOG000286146.
KOiK11258.
OMAiTRHRGFQ.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.
BioCyciEcoCyc:SMALLILVM-MONOMER.
ECOL316407:JW3742-MONOMER.
MetaCyc:SMALLILVM-MONOMER.
SABIO-RKP0ADG1.

Miscellaneous databases

PROiP0ADG1.

Family and domain databases

InterProiIPR002912. ACT_dom.
[Graphical view]
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILVM_ECOLI
AccessioniPrimary (citable) accession number: P0ADG1
Secondary accession number(s): P13048, P78269, Q2M878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.