P0ADG1 (ILVM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase isozyme 2 small subunit
EC=2.2.1.6

Alternative name(s):
ALS-II
Acetohydroxy-acid synthase II small subunit
Short name=AHAS-II

Gene names

Name:	ilvM
Ordered Locus Names:	b3769, JW3742

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Tetramer of two large and two small chains.
Miscellaneous	E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.
Sequence caution	The sequence AAB59051.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA26261.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	Magnesium Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid biosynthetic process Inferred from direct assay PubMed 9581571. Source: EcoCyc isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway valine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acetolactate synthase activity Inferred from direct assay PubMed 9581571. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 87	87	Acetolactate synthase isozyme 2 small subunit		PRO_0000151429

Sequences

	Sequence		Length	Mass (Da)
	P0ADG1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: CEB6B5211262E0AF	FASTA	87	9,703
	10 20 30 40 50 60 MMQHQVNVSA RFNPETLERV LRVVRHRGFH VCSMNMAAAS DAQNINIELT VASPRSVDLL 70 80 FSQLNKLVDV AHVAICQSTT TSQQIRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence." Kuramitsu S., Ogawa T., Ogawa H., Kagamiyama H. J. Biochem. 97:993-999(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12." Lawther R.P., Wek R.C., Lopes J.M., Pereira R., Taillon B.E., Hatfield G.W. Nucleic Acids Res. 15:2137-2155(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[3]	"DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli." Pagel J.M., Winkelman J.W., Adams C.W., Hatfield G.W. J. Mol. Biol. 224:919-935(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02413 Genomic DNA. Translation: CAA26261.1. Different initiation. M10313 Genomic DNA. Translation: AAB59051.1. Different initiation. X04890 Genomic DNA. Translation: CAA28574.1. M87049 Genomic DNA. Translation: AAA67572.1. U00096 Genomic DNA. Translation: AAC77489.1. AP009048 Genomic DNA. Translation: BAE77528.1.
RefSeq	NP_418217.1. NC_000913.2. YP_491669.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ADG1.
SMR	P0ADG1. Positions 2-84.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0ADG1. 2 interactions.
STRING	511145.b3769.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77489; AAC77489; b3769. BAE77528; BAE77528; BAE77528.
GeneID	12934298. 948279.
KEGG	ecj:Y75_p3406. eco:b3769.
PATRIC	32123033. VBIEscCol129921_3886.
Organism-specific databases
EchoBASE	EB0496.
EcoGene	EG10501. ilvM.
Phylogenomic databases
eggNOG	COG3978.
HOGENOM	HOG000286146.
KO	K11258.
OMA	MMQHQVA.
ProtClustDB	PRK11152.
Enzyme and pathway databases
BioCyc	EcoCyc:SMALLILVM-MONOMER. ECOL316407:JW3742-MONOMER. MetaCyc:SMALLILVM-MONOMER.
SABIO-RK	P0ADG1.
UniPathway	UPA00047; UER00055. UPA00049; UER00059.
Gene expression databases
Genevestigator	P0ADG1.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ILVM_ECOLI

Accession

Primary (citable) accession number: P0ADG1
Secondary accession number(s): P13048, P78269, Q2M878

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program