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Protein

Phosphogluconate dehydratase

Gene

edd

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of 6-phospho-D-gluconate to 2-dehydro-3-deoxy-6-phospho-D-gluconate.UniRule annotation2 Publications

Catalytic activityi

6-phospho-D-gluconate = 2-dehydro-3-deoxy-6-phospho-D-gluconate + H2O.UniRule annotation2 Publications

Cofactori

[4Fe-4S] clusterUniRule annotationBy similarity1 PublicationNote: Binds 1 [4Fe-4S] cluster.UniRule annotationBy similarity

Enzyme regulationi

Sensitive to oxidants such as superoxide or hydrogen peroxide.2 Publications

Pathwayi: Entner-Doudoroff pathway

This protein is involved in the pathway Entner-Doudoroff pathway, which is part of Carbohydrate metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway Entner-Doudoroff pathway and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi154Iron-sulfur (4Fe-4S)UniRule annotationBy similarity1
Metal bindingi221Iron-sulfur (4Fe-4S)UniRule annotationBy similarity1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphogluconate dehydratase activity Source: EcoliWiki

GO - Biological processi

  • D-gluconate catabolic process Source: EcoCyc
  • Entner-Doudoroff pathway through 6-phosphogluconate Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLyase
Biological processCarbohydrate metabolism, Gluconate utilization
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:PGLUCONDEHYDRAT-MONOMER
MetaCyc:PGLUCONDEHYDRAT-MONOMER
UniPathwayiUPA00226

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphogluconate dehydrataseUniRule annotationCurated (EC:4.2.1.12UniRule annotation2 Publications)
Alternative name(s):
6-phosphogluconate dehydratase1 Publication
Entner-Doudoroff dehydrase1 Publication
Gene namesi
Name:edd1 PublicationUniRule annotation
Ordered Locus Names:b1851, JW1840
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10257 edd

Pathology & Biotechi

Disruption phenotypei

The mutant grows at normal rates on glucose and fructose, whereas on gluconate it grows about one-third as fast as the wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001035531 – 603Phosphogluconate dehydrataseAdd BLAST603

Proteomic databases

PaxDbiP0ADF6
PRIDEiP0ADF6

Expressioni

Inductioni

By growth on gluconate.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261382, 12 interactors
DIPiDIP-47906N
IntActiP0ADF6, 12 interactors
STRINGi316385.ECDH10B_1992

Structurei

3D structure databases

ProteinModelPortaliP0ADF6
SMRiP0ADF6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IlvD/Edd family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C01 Bacteria
COG0129 LUCA
HOGENOMiHOG000173157
InParanoidiP0ADF6
KOiK01690
OMAiCANIAHV
PhylomeDBiP0ADF6

Family and domain databases

HAMAPiMF_02094 Edd, 1 hit
InterProiView protein in InterPro
IPR004786 6-phosphgluc_deHydtase
IPR000581 DiOHA_6PGluconate_deHydtase
IPR020558 DiOHA_6PGluconate_deHydtase_CS
IPR037237 IlvD/EDD_N
PANTHERiPTHR43661:SF1 PTHR43661:SF1, 1 hit
PfamiView protein in Pfam
PF00920 ILVD_EDD, 1 hit
SUPFAMiSSF143975 SSF143975, 1 hit
TIGRFAMsiTIGR01196 edd, 1 hit
PROSITEiView protein in PROSITE
PS00886 ILVD_EDD_1, 1 hit
PS00887 ILVD_EDD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0ADF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPQLLRVTN RIIERSRETR SAYLARIEQA KTSTVHRSQL ACGNLAHGFA
60 70 80 90 100
ACQPEDKASL KSMLRNNIAI ITSYNDMLSA HQPYEHYPEI IRKALHEANA
110 120 130 140 150
VGQVAGGVPA MCDGVTQGQD GMELSLLSRE VIAMSAAVGL SHNMFDGALF
160 170 180 190 200
LGVCDKIVPG LTMAALSFGH LPAVFVPSGP MASGLPNKEK VRIRQLYAEG
210 220 230 240 250
KVDRMALLES EAASYHAPGT CTFYGTANTN QMVVEFMGMQ LPGSSFVHPD
260 270 280 290 300
SPLRDALTAA AARQVTRMTG NGNEWMPIGK MIDEKVVVNG IVALLATGGS
310 320 330 340 350
TNHTMHLVAM ARAAGIQINW DDFSDLSDVV PLMARLYPNG PADINHFQAA
360 370 380 390 400
GGVPVLVREL LKAGLLHEDV NTVAGFGLSR YTLEPWLNNG ELDWREGAEK
410 420 430 440 450
SLDSNVIASF EQPFSHHGGT KVLSGNLGRA VMKTSAVPVE NQVIEAPAVV
460 470 480 490 500
FESQHDVMPA FEAGLLDRDC VVVVRHQGPK ANGMPELHKL MPPLGVLLDR
510 520 530 540 550
CFKIALVTDG RLSGASGKVP SAIHVTPEAY DGGLLAKVRD GDIIRVNGQT
560 570 580 590 600
GELTLLVDEA ELAAREPHIP DLSASRVGTG RELFSALREK LSGAEQGATC

ITF
Length:603
Mass (Da):64,639
Last modified:December 6, 2005 - v1
Checksum:iD046405193BFC185
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252 – 254PLR → AC in AAA23722 (PubMed:1624451).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87458 Genomic DNA Translation: AAA23722.1
X63694 Genomic DNA Translation: CAA45221.1
U00096 Genomic DNA Translation: AAC74921.1
AP009048 Genomic DNA Translation: BAA15659.1
L20897 Genomic DNA Translation: AAA23863.1
PIRiA42986
RefSeqiNP_416365.1, NC_000913.3
WP_001069467.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74921; AAC74921; b1851
BAA15659; BAA15659; BAA15659
GeneIDi946362
KEGGiecj:JW1840
eco:b1851
PATRICifig|511145.12.peg.1929

Similar proteinsi

Entry informationi

Entry nameiEDD_ECOLI
AccessioniPrimary (citable) accession number: P0ADF6
Secondary accession number(s): P25530
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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