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Protein

Translocation and assembly module TamA

Gene

tamA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the translocation and assembly module (TAM) autotransporter assembly complex, which functions in translocation of autotransporters across the outer membrane. Has anion selective channel-forming ability, but the physiological relevance of this activity is unclear.1 Publication

GO - Biological processi

  • protein secretion Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7874-MONOMER.
ECOL316407:JW4179-MONOMER.

Protein family/group databases

TCDBi1.B.33.2.4. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocation and assembly module TamA
Alternative name(s):
Autotransporter assembly factor TamA
Gene namesi
Name:tamA
Synonyms:yftM, ytfM
Ordered Locus Names:b4220, JW4179
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12513. tamA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei447 – 4548Beta strandedSequence analysis
Transmembranei465 – 4728Beta strandedSequence analysis
Transmembranei476 – 4838Beta strandedSequence analysis
Transmembranei500 – 51011Beta strandedSequence analysisAdd
BLAST
Transmembranei514 – 52310Beta strandedSequence analysis
Transmembranei535 – 54410Beta strandedSequence analysis
Transmembranei551 – 5599Beta strandedSequence analysis
Transmembranei567 – 57711Beta strandedSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of cell outer membrane Source: EcoliWiki
  • TAM protein secretion complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Not essential, cells grow more slowly than wild-type. No changes in permeability or outer membrane integrity. Loss of localization of an exogenous protein, P1121 (ROD_p1121 of Citrobacter rodentium), to outer membranes, loss of cell aggregation when adhesins are over-expressed in a double tamA-tamB deletion mutant.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 577556Translocation and assembly module TamAPRO_0000013946Add
BLAST

Proteomic databases

PaxDbiP0ADE4.
PRIDEiP0ADE4.

Interactioni

Subunit structurei

Interacts with TamB.1 Publication

Protein-protein interaction databases

BioGridi4259308. 242 interactions.
DIPiDIP-51058N.
IntActiP0ADE4. 6 interactions.
STRINGi511145.b4220.

Structurei

Secondary structure

1
577
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 305Combined sources
Helixi34 – 4310Combined sources
Beta strandi49 – 513Combined sources
Beta strandi54 – 574Combined sources
Helixi58 – 7114Combined sources
Beta strandi74 – 763Combined sources
Beta strandi78 – 803Combined sources
Beta strandi94 – 996Combined sources
Beta strandi105 – 11410Combined sources
Helixi115 – 1195Combined sources
Helixi121 – 1277Combined sources
Helixi139 – 15618Combined sources
Beta strandi162 – 17110Combined sources
Turni172 – 1754Combined sources
Beta strandi176 – 1849Combined sources
Beta strandi190 – 1989Combined sources
Helixi203 – 2075Combined sources
Helixi220 – 23213Combined sources
Beta strandi236 – 2438Combined sources
Helixi245 – 2473Combined sources
Turni248 – 2514Combined sources
Beta strandi253 – 26210Combined sources
Beta strandi267 – 2759Combined sources
Turni276 – 2783Combined sources
Beta strandi279 – 28911Combined sources
Beta strandi297 – 31822Combined sources
Turni323 – 3253Combined sources
Beta strandi326 – 33914Combined sources
Beta strandi342 – 35615Combined sources
Beta strandi360 – 37617Combined sources
Beta strandi379 – 40224Combined sources
Beta strandi404 – 41613Combined sources
Helixi418 – 4203Combined sources
Beta strandi426 – 43914Combined sources
Turni440 – 4423Combined sources
Beta strandi443 – 45715Combined sources
Helixi459 – 4613Combined sources
Helixi464 – 4663Combined sources
Turni473 – 4753Combined sources
Beta strandi496 – 51116Combined sources
Beta strandi514 – 52714Combined sources
Beta strandi535 – 54612Combined sources
Beta strandi549 – 56012Combined sources
Beta strandi568 – 5725Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LY3NMR-122-102[»]
4BZAX-ray1.84A22-275[»]
4C00X-ray2.25A22-577[»]
ProteinModelPortaliP0ADE4.
SMRiP0ADE4. Positions 22-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 26377POTRAPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni244 – 577334Sufficient for outer membrane targetingAdd
BLAST

Domaini

Sequence similaritiesi

Belongs to the TamA family.Curated
Contains 1 POTRA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CFU. Bacteria.
COG0729. LUCA.
HOGENOMiHOG000275353.
InParanoidiP0ADE4.
KOiK07278.
OMAiWDSSSGW.
PhylomeDBiP0ADE4.

Family and domain databases

InterProiIPR000184. Bac_surfAg_D15.
IPR010827. Surface_Ag_variable_number.
[Graphical view]
PfamiPF01103. Bac_surface_Ag. 1 hit.
PF07244. POTRA. 1 hit.
[Graphical view]
PROSITEiPS51779. POTRA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ADE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYIRQLCCV SLLCLSGSAV AANVRLQVEG LSGQLEKNVR AQLSTIESDE
60 70 80 90 100
VTPDRRFRAR VDDAIREGLK ALGYYQPTIE FDLRPPPKKG RQVLIAKVTP
110 120 130 140 150
GVPVLIGGTD VVLRGGARTD KDYLKLLDTR PAIGTVLNQG DYENFKKSLT
160 170 180 190 200
SIALRKGYFD SEFTKAQLGI ALGLHKAFWD IDYNSGERYR FGHVTFEGSQ
210 220 230 240 250
IRDEYLQNLV PFKEGDEYES KDLAELNRRL SATGWFNSVV VAPQFDKARE
260 270 280 290 300
TKVLPLTGVV SPRTENTIET GVGYSTDVGP RVKATWKKPW MNSYGHSLTT
310 320 330 340 350
STSISAPEQT LDFSYKMPLL KNPLEQYYLV QGGFKRTDLN DTESDSTTLV
360 370 380 390 400
ASRYWDLSSG WQRAINLRWS LDHFTQGEIT NTTMLFYPGV MISRTRSRGG
410 420 430 440 450
LMPTWGDSQR YSIDYSNTAW GSDVDFSVFQ AQNVWIRTLY DRHRFVTRGT
460 470 480 490 500
LGWIETGDFD KVPPDLRFFA GGDRSIRGYK YKSIAPKYAN GDLKGASKLI
510 520 530 540 550
TGSLEYQYNV TGKWWGAVFV DSGEAVSDIR RSDFKTGTGV GVRWESPVGP
560 570
IKLDFAVPVA DKDEHGLQFY IGLGPEL
Length:577
Mass (Da):64,796
Last modified:December 6, 2005 - v1
Checksum:i253D1D5BEA744D25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97116.1.
U00096 Genomic DNA. Translation: AAC77177.1.
AP009048 Genomic DNA. Translation: BAE78221.1.
PIRiS56445.
RefSeqiNP_418641.1. NC_000913.3.
WP_001269327.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77177; AAC77177; b4220.
BAE78221; BAE78221; BAE78221.
GeneIDi948733.
KEGGiecj:JW4179.
eco:b4220.
PATRICi32124017. VBIEscCol129921_4353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97116.1.
U00096 Genomic DNA. Translation: AAC77177.1.
AP009048 Genomic DNA. Translation: BAE78221.1.
PIRiS56445.
RefSeqiNP_418641.1. NC_000913.3.
WP_001269327.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LY3NMR-122-102[»]
4BZAX-ray1.84A22-275[»]
4C00X-ray2.25A22-577[»]
ProteinModelPortaliP0ADE4.
SMRiP0ADE4. Positions 22-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259308. 242 interactions.
DIPiDIP-51058N.
IntActiP0ADE4. 6 interactions.
STRINGi511145.b4220.

Protein family/group databases

TCDBi1.B.33.2.4. the outer membrane protein insertion porin (bam complex) (ompip) family.

Proteomic databases

PaxDbiP0ADE4.
PRIDEiP0ADE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77177; AAC77177; b4220.
BAE78221; BAE78221; BAE78221.
GeneIDi948733.
KEGGiecj:JW4179.
eco:b4220.
PATRICi32124017. VBIEscCol129921_4353.

Organism-specific databases

EchoBASEiEB2406.
EcoGeneiEG12513. tamA.

Phylogenomic databases

eggNOGiENOG4105CFU. Bacteria.
COG0729. LUCA.
HOGENOMiHOG000275353.
InParanoidiP0ADE4.
KOiK07278.
OMAiWDSSSGW.
PhylomeDBiP0ADE4.

Enzyme and pathway databases

BioCyciEcoCyc:G7874-MONOMER.
ECOL316407:JW4179-MONOMER.

Miscellaneous databases

PROiP0ADE4.

Family and domain databases

InterProiIPR000184. Bac_surfAg_D15.
IPR010827. Surface_Ag_variable_number.
[Graphical view]
PfamiPF01103. Bac_surface_Ag. 1 hit.
PF07244. POTRA. 1 hit.
[Graphical view]
PROSITEiPS51779. POTRA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAMA_ECOLI
AccessioniPrimary (citable) accession number: P0ADE4
Secondary accession number(s): P39320, Q2M685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.