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Protein

LPS-assembly lipoprotein LptE

Gene

lptE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Together with LptD, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Required for the proper assembly of LptD. Binds LPS and may serve as the LPS recognition site at the outer membrane.UniRule annotation3 Publications

GO - Molecular functioni

  • lipopolysaccharide binding Source: EcoCyc

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
  • lipopolysaccharide transport Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10855-MONOMER.
ECOL316407:JW0636-MONOMER.
MetaCyc:EG10855-MONOMER.

Protein family/group databases

TCDBi1.B.42.1.2. the outer membrane lipopolysaccharide export porin (lps-ep) family.

Names & Taxonomyi

Protein namesi
Recommended name:
LPS-assembly lipoprotein LptEUniRule annotation
Alternative name(s):
Rare lipoprotein B
Gene namesi
Name:lptEUniRule annotation
Synonyms:rlpB
Ordered Locus Names:b0641, JW0636
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10855. lptE.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi117 – 1193PIS → R: Affects interaction with LptD and LptD biogenesis. Increases outer membrane permeability. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Chaini19 – 193175LPS-assembly lipoprotein LptEPRO_0000018185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi19 – 191N-palmitoyl cysteineUniRule annotation
Lipidationi19 – 191S-diacylglycerol cysteineUniRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0ADC1.
PaxDbiP0ADC1.
PRIDEiP0ADC1.

Interactioni

Subunit structurei

Component of the lipopolysaccharide transport and assembly complex. Interacts with LptD. May interact with LptD during its assembly by the beta-barrel assembly machine. Directly contacts LptD at a wide range of positions, encompassing multiple surfaces of LptE. In one specific interaction, LptE contacts a predicted extracellular loop of LptD through the lumen of the beta-barrel. This specific interaction is required for proper folding of LptD and assembly of the complex.UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
lptDP315543EBI-1119442,EBI-549369

Protein-protein interaction databases

BioGridi4260757. 223 interactions.
DIPiDIP-35987N.
IntActiP0ADC1. 6 interactions.
STRINGi511145.b0641.

Structurei

Secondary structure

1
193
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 333Combined sources
Beta strandi34 – 418Combined sources
Helixi46 – 5712Combined sources
Beta strandi61 – 633Combined sources
Turni69 – 713Combined sources
Beta strandi74 – 11037Combined sources
Turni111 – 1133Combined sources
Beta strandi114 – 12613Combined sources
Helixi130 – 1323Combined sources
Helixi140 – 15617Combined sources
Helixi159 – 16810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NHRX-ray2.34A20-182[»]
4RH8X-ray2.20A/B/C/D20-186[»]
4RHBX-ray3.35B/D19-193[»]
ProteinModelPortaliP0ADC1.
SMRiP0ADC1. Positions 20-169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LptE lipoprotein family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108U2H. Bacteria.
COG2980. LUCA.
HOGENOMiHOG000256192.
KOiK03643.
OMAiRKLLTVH.

Family and domain databases

HAMAPiMF_01186. LPS_assembly_LptE. 1 hit.
InterProiIPR007485. LPS_assembly_LptE.
[Graphical view]
PfamiPF04390. LptE. 1 hit.
[Graphical view]
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ADC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYLATLLLS LAVLITAGCG WHLRDTTQVP STMKVMILDS GDPNGPLSRA
60 70 80 90 100
VRNQLRLNGV ELLDKETTRK DVPSLRLGKV SIAKDTASVF RNGQTAEYQM
110 120 130 140 150
IMTVNATVLI PGRDIYPISA KVFRSFFDNP QMALAKDNEQ DMIVKEMYDR
160 170 180 190
AAEQLIRKLP SIRAADIRSD EEQTSTTTDT PATPARVSTT LGN
Length:193
Mass (Da):21,357
Last modified:December 6, 2005 - v1
Checksum:iC9387BC2008ADEDC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171P → S in AAA24554 (PubMed:3316191).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18277 Genomic DNA. Translation: AAA24554.1.
U82598 Genomic DNA. Translation: AAB40842.1.
U00096 Genomic DNA. Translation: AAC73742.1.
AP009048 Genomic DNA. Translation: BAA35288.1.
PIRiG64798. LPECRB.
RefSeqiNP_415174.1. NC_000913.3.
WP_001269673.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73742; AAC73742; b0641.
BAA35288; BAA35288; BAA35288.
GeneIDi946257.
KEGGiecj:JW0636.
eco:b0641.
PATRICi32116465. VBIEscCol129921_0672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18277 Genomic DNA. Translation: AAA24554.1.
U82598 Genomic DNA. Translation: AAB40842.1.
U00096 Genomic DNA. Translation: AAC73742.1.
AP009048 Genomic DNA. Translation: BAA35288.1.
PIRiG64798. LPECRB.
RefSeqiNP_415174.1. NC_000913.3.
WP_001269673.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NHRX-ray2.34A20-182[»]
4RH8X-ray2.20A/B/C/D20-186[»]
4RHBX-ray3.35B/D19-193[»]
ProteinModelPortaliP0ADC1.
SMRiP0ADC1. Positions 20-169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260757. 223 interactions.
DIPiDIP-35987N.
IntActiP0ADC1. 6 interactions.
STRINGi511145.b0641.

Protein family/group databases

TCDBi1.B.42.1.2. the outer membrane lipopolysaccharide export porin (lps-ep) family.

Proteomic databases

EPDiP0ADC1.
PaxDbiP0ADC1.
PRIDEiP0ADC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73742; AAC73742; b0641.
BAA35288; BAA35288; BAA35288.
GeneIDi946257.
KEGGiecj:JW0636.
eco:b0641.
PATRICi32116465. VBIEscCol129921_0672.

Organism-specific databases

EchoBASEiEB0848.
EcoGeneiEG10855. lptE.

Phylogenomic databases

eggNOGiENOG4108U2H. Bacteria.
COG2980. LUCA.
HOGENOMiHOG000256192.
KOiK03643.
OMAiRKLLTVH.

Enzyme and pathway databases

BioCyciEcoCyc:EG10855-MONOMER.
ECOL316407:JW0636-MONOMER.
MetaCyc:EG10855-MONOMER.

Miscellaneous databases

PROiP0ADC1.

Family and domain databases

HAMAPiMF_01186. LPS_assembly_LptE. 1 hit.
InterProiIPR007485. LPS_assembly_LptE.
[Graphical view]
PfamiPF04390. LptE. 1 hit.
[Graphical view]
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLPTE_ECOLI
AccessioniPrimary (citable) accession number: P0ADC1
Secondary accession number(s): P10101, P77576
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.