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Protein

Murein hydrolase activator NlpD

Gene

nlpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.2 Publications

GO - Biological processi

  • autolysis Source: EcoliWiki
  • cell separation after cytokinesis Source: EcoliWiki
  • division septum assembly Source: EcoCyc
  • positive regulation of hydrolase activity Source: EcoCyc
  • response to drug Source: EcoCyc

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG12111-MONOMER
MetaCyc:EG12111-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Murein hydrolase activator NlpD
Gene namesi
Name:nlpD
Ordered Locus Names:b2742, JW2712
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12111 nlpD

Subcellular locationi

  • Cell inner membrane Curated; Lipid-anchor PROSITE-ProRule annotation
  • Note: Localizes at the septal ring.1 Publication

GO - Cellular componenti

  • cell division site Source: EcoliWiki
  • cell outer membrane Source: EcoCyc
  • cytosol Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells are shorter in a single mutant, double envC-nlpD disruptions have defects in septation and cell separation and form long filaments (8-fold longer). Cell length increase is more exacerbated with a triple mepM (yebA) or ygeR disruption (15-fold longer) and further yet by the quadruple disruption mutant (envC-nlpD-mepM(yebA)-ygeR, over 21-fold longer). Quadruple mutants are less sensitive to ampicillin lysis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000001802926 – 379Murein hydrolase activator NlpDAdd BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi26N-palmitoyl cysteinePROSITE-ProRule annotation1 Publication1
Lipidationi26S-diacylglycerol cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP0ADA3
PRIDEiP0ADA3

2D gel databases

SWISS-2DPAGEP0ADA3

Interactioni

Protein-protein interaction databases

BioGridi4261894, 278 interactors
DIPiDIP-48067N
IntActiP0ADA3, 4 interactors
STRINGi316385.ECDH10B_2910

Structurei

3D structure databases

ProteinModelPortaliP0ADA3
SMRiP0ADA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati66 – 731-18
Repeati74 – 811-2; approximate8
Repeati82 – 891-38
Repeati90 – 971-4; approximate8
Domaini121 – 165LysMPROSITE-ProRule annotationAdd BLAST45
Repeati205 – 2112-17
Repeati227 – 2332-27
Repeati239 – 2452-37
Repeati246 – 2522-47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 974 X 8 AA tandem repeats of Q-Q-P-Q-I-Q-P-VAdd BLAST32
Regioni205 – 2524 X 7 AA approximate repeatsAdd BLAST48

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108K5P Bacteria
COG0739 LUCA
HOGENOMiHOG000159026
InParanoidiP0ADA3
KOiK06194
OMAiTMFLIAY
PhylomeDBiP0ADA3

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR011055 Dup_hybrid_motif
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR016047 Peptidase_M23
PfamiView protein in Pfam
PF01476 LysM, 1 hit
PF01551 Peptidase_M23, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF51261 SSF51261, 1 hit
PROSITEiView protein in PROSITE
PS51782 LYSM, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ADA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGSPKFTV RRIAALSLVS LWLAGCSDTS NPPAPVSSVN GNAPANTNSG
60 70 80 90 100
MLITPPPKMG TTSTAQQPQI QPVQQPQIQA TQQPQIQPVQ PVAQQPVQME
110 120 130 140 150
NGRIVYNRQY GNIPKGSYSG STYTVKKGDT LFYIAWITGN DFRDLAQRNN
160 170 180 190 200
IQAPYALNVG QTLQVGNASG TPITGGNAIT QADAAEQGVV IKPAQNSTVA
210 220 230 240 250
VASQPTITYS ESSGEQSANK MLPNNKPTAT TVTAPVTVPT ASTTEPTVSS
260 270 280 290 300
TSTSTPISTW RWPTEGKVIE TFGASEGGNK GIDIAGSKGQ AIIATADGRV
310 320 330 340 350
VYAGNALRGY GNLIIIKHND DYLSAYAHND TMLVREQQEV KAGQKIATMG
360 370
STGTSSTRLH FEIRYKGKSV NPLRYLPQR
Length:379
Mass (Da):40,149
Last modified:December 6, 2005 - v1
Checksum:iA8E6A2B8456105FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139G → A in BAA04487 (PubMed:8208244).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07869 Unassigned DNA Translation: AAA17875.1
U29579 Genomic DNA Translation: AAA69252.1
U00096 Genomic DNA Translation: AAC75784.1
AP009048 Genomic DNA Translation: BAE76819.1
D17549 Genomic DNA Translation: BAA04487.1
PIRiB55522
RefSeqiNP_417222.1, NC_000913.3
WP_001272592.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75784; AAC75784; b2742
BAE76819; BAE76819; BAE76819
GeneIDi947011
KEGGiecj:JW2712
eco:b2742
PATRICifig|1411691.4.peg.3998

Similar proteinsi

Entry informationi

Entry nameiNLPD_ECOLI
AccessioniPrimary (citable) accession number: P0ADA3
Secondary accession number(s): P33648, Q2MA87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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