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Protein

Acyl-CoA thioesterase I

Gene

tesA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361NucleophileBy similarity
Binding sitei70 – 701Substrate; via amide nitrogenBy similarity
Binding sitei99 – 991SubstrateBy similarity
Active sitei180 – 1801By similarity
Active sitei183 – 1831By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA thioesterase I (EC:3.1.2.-)
Alternative name(s):
Lecithinase B
Lysophospholipase L1 (EC:3.1.1.5)
Protease I
Gene namesi
Name:tesA
Ordered Locus Names:c0615
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 208182Acyl-CoA thioesterase IPRO_0000043365Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi199310.c0615.

Structurei

3D structure databases

ProteinModelPortaliP0ADA2.
SMRiP0ADA2. Positions 27-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'GDSL' lipolytic enzyme family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000261382.
KOiK10804.
OMAiRGFPPQQ.
OrthoDBiEOG6PGK4B.

Family and domain databases

Gene3Di3.40.50.1110. 1 hit.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 1 hit.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 1 hit.
PROSITEiPS01098. LIPASE_GDSL_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ADA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNFNNVFRW HLPFLFLVLL TFRAAAADTL LILGDSLSAG YRMSASAAWP
60 70 80 90 100
ALLNDKWQSK TSVVNASISG DTSQQGLARL PALLKQHQPR WVLVELGGND
110 120 130 140 150
GLRGFQPQQT EQTLRQILQD VKAANAEPLL MQIRLPANYG RRYNEAFSAI
160 170 180 190 200
YPKLAKEFDV PLLPFFMEEV YLKPQWMQDD GIHPNRDAQP FIADWMAKQL

QPLVNHDS
Length:208
Mass (Da):23,622
Last modified:December 6, 2005 - v1
Checksum:iCD03F23EA39541F1
GO

Sequence cautioni

The sequence AAN79092.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79092.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiAAN79092; AAN79092; c0615.
KEGGiecc:c0615.
PATRICi18279271. VBIEscCol75197_0580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79092.1. Different initiation.

3D structure databases

ProteinModelPortaliP0ADA2.
SMRiP0ADA2. Positions 27-204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN79092; AAN79092; c0615.
KEGGiecc:c0615.
PATRICi18279271. VBIEscCol75197_0580.

Phylogenomic databases

HOGENOMiHOG000261382.
KOiK10804.
OMAiRGFPPQQ.
OrthoDBiEOG6PGK4B.

Family and domain databases

Gene3Di3.40.50.1110. 1 hit.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 1 hit.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 1 hit.
PROSITEiPS01098. LIPASE_GDSL_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiTESA_ECOL6
AccessioniPrimary (citable) accession number: P0ADA2
Secondary accession number(s): P29679, P37331, P77125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 24, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.