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Protein

Leu/Ile/Val-binding protein

Gene

livJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids.

GO - Molecular functioni

  • branched-chain amino acid transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • branched-chain amino acid transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:LIVJ-MONOMER.
ECOL316407:JW3425-MONOMER.
MetaCyc:LIVJ-MONOMER.

Protein family/group databases

TCDBi3.A.1.4.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Leu/Ile/Val-binding protein
Short name:
LIV-BP
Gene namesi
Name:livJ
Ordered Locus Names:b3460, JW3425
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10539. livJ.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 233 PublicationsAdd BLAST23
ChainiPRO_000001770024 – 367Leu/Ile/Val-binding proteinAdd BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi76 ↔ 101

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0AD96.
PaxDbiP0AD96.
PRIDEiP0AD96.

2D gel databases

SWISS-2DPAGEP0AD96.

Interactioni

Protein-protein interaction databases

BioGridi4259328. 18 interactors.
IntActiP0AD96. 4 interactors.
STRINGi511145.b3460.

Structurei

Secondary structure

1367
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 32Combined sources7
Beta strandi34 – 36Combined sources3
Helixi39 – 58Combined sources20
Turni59 – 61Combined sources3
Beta strandi67 – 73Combined sources7
Helixi78 – 90Combined sources13
Beta strandi95 – 97Combined sources3
Helixi102 – 115Combined sources14
Beta strandi118 – 123Combined sources6
Helixi127 – 130Combined sources4
Beta strandi135 – 141Combined sources7
Helixi144 – 146Combined sources3
Helixi147 – 157Combined sources11
Beta strandi162 – 168Combined sources7
Helixi172 – 187Combined sources16
Beta strandi192 – 197Combined sources6
Helixi206 – 214Combined sources9
Beta strandi219 – 224Combined sources6
Helixi226 – 238Combined sources13
Beta strandi244 – 247Combined sources4
Helixi249 – 251Combined sources3
Helixi254 – 260Combined sources7
Helixi261 – 264Combined sources4
Beta strandi268 – 271Combined sources4
Helixi275 – 277Combined sources3
Helixi279 – 281Combined sources3
Helixi282 – 290Combined sources9
Helixi298 – 314Combined sources17
Helixi320 – 329Combined sources10
Beta strandi332 – 334Combined sources3
Beta strandi337 – 339Combined sources3
Beta strandi347 – 349Combined sources3
Beta strandi353 – 357Combined sources5
Beta strandi363 – 365Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z15X-ray1.70A24-367[»]
1Z16X-ray1.72A24-367[»]
1Z17X-ray1.96A24-367[»]
1Z18X-ray2.10A24-367[»]
2LIVX-ray2.40A24-367[»]
ProteinModelPortaliP0AD96.
SMRiP0AD96.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AD96.

Family & Domainsi

Sequence similaritiesi

Belongs to the leucine-binding protein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CP9. Bacteria.
COG0683. LUCA.
HOGENOMiHOG000130989.
InParanoidiP0AD96.
KOiK01999.
OMAiLWNVARV.
PhylomeDBiP0AD96.

Family and domain databases

InterProiIPR028081. Leu-bd.
IPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR30483:SF16. PTHR30483:SF16. 1 hit.
PfamiPF13458. Peripla_BP_6. 1 hit.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKGKALLA GCIALAFSNM ALAEDIKVAV VGAMSGPVAQ YGDQEFTGAE
60 70 80 90 100
QAVADINAKG GIKGNKLQIV KYDDACDPKQ AVAVANKVVN DGIKYVIGHL
110 120 130 140 150
CSSSTQPASD IYEDEGILMI TPAATAPELT ARGYQLILRT TGLDSDQGPT
160 170 180 190 200
AAKYILEKVK PQRIAIVHDK QQYGEGLARA VQDGLKKGNA NVVFFDGITA
210 220 230 240 250
GEKDFSTLVA RLKKENIDFV YYGGYHPEMG QILRQARAAG LKTQFMGPEG
260 270 280 290 300
VANVSLSNIA GESAEGLLVT KPKNYDQVPA NKPIVDAIKA KKQDPSGAFV
310 320 330 340 350
WTTYAALQSL QAGLNQSDDP AEIAKYLKAN SVDTVMGPLT WDEKGDLKGF
360
EFGVFDWHAN GTATDAK
Length:367
Mass (Da):39,076
Last modified:December 6, 2005 - v1
Checksum:i63F0729C251F18FD
GO

Sequence cautioni

The sequence AAB18435 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3I → T (PubMed:3891753).Curated1
Sequence conflicti3I → T (PubMed:4077929).Curated1
Sequence conflicti3I → T (PubMed:2195019).Curated1
Sequence conflicti12C → L (PubMed:3891753).Curated1
Sequence conflicti12C → L (PubMed:4077929).Curated1
Sequence conflicti12C → L (PubMed:2195019).Curated1
Sequence conflicti16A → G (PubMed:3891753).Curated1
Sequence conflicti70V → A (PubMed:3891753).Curated1
Sequence conflicti70V → A (PubMed:4077929).Curated1
Sequence conflicti70V → A (PubMed:2195019).Curated1
Sequence conflicti70V → A (PubMed:328304).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05516 Genomic DNA. Translation: AAA83881.1.
M29377 Genomic DNA. Translation: AAA24075.1.
U00039 Genomic DNA. Translation: AAB18435.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48185.1.
AP009048 Genomic DNA. Translation: BAE77833.1.
PIRiG65142. BLEC.
RefSeqiYP_026223.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48185; AAT48185; b3460.
BAE77833; BAE77833; BAE77833.
GeneIDi947971.
KEGGiecj:JW3425.
eco:b3460.
PATRICi32122364. VBIEscCol129921_3559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05516 Genomic DNA. Translation: AAA83881.1.
M29377 Genomic DNA. Translation: AAA24075.1.
U00039 Genomic DNA. Translation: AAB18435.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48185.1.
AP009048 Genomic DNA. Translation: BAE77833.1.
PIRiG65142. BLEC.
RefSeqiYP_026223.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z15X-ray1.70A24-367[»]
1Z16X-ray1.72A24-367[»]
1Z17X-ray1.96A24-367[»]
1Z18X-ray2.10A24-367[»]
2LIVX-ray2.40A24-367[»]
ProteinModelPortaliP0AD96.
SMRiP0AD96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259328. 18 interactors.
IntActiP0AD96. 4 interactors.
STRINGi511145.b3460.

Protein family/group databases

TCDBi3.A.1.4.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AD96.

Proteomic databases

EPDiP0AD96.
PaxDbiP0AD96.
PRIDEiP0AD96.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48185; AAT48185; b3460.
BAE77833; BAE77833; BAE77833.
GeneIDi947971.
KEGGiecj:JW3425.
eco:b3460.
PATRICi32122364. VBIEscCol129921_3559.

Organism-specific databases

EchoBASEiEB0534.
EcoGeneiEG10539. livJ.

Phylogenomic databases

eggNOGiENOG4105CP9. Bacteria.
COG0683. LUCA.
HOGENOMiHOG000130989.
InParanoidiP0AD96.
KOiK01999.
OMAiLWNVARV.
PhylomeDBiP0AD96.

Enzyme and pathway databases

BioCyciEcoCyc:LIVJ-MONOMER.
ECOL316407:JW3425-MONOMER.
MetaCyc:LIVJ-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AD96.
PROiP0AD96.

Family and domain databases

InterProiIPR028081. Leu-bd.
IPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR30483:SF16. PTHR30483:SF16. 1 hit.
PfamiPF13458. Peripla_BP_6. 1 hit.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLIVJ_ECOLI
AccessioniPrimary (citable) accession number: P0AD96
Secondary accession number(s): P02917
, P76698, Q2M7C3, Q8CVL7, Q8X6S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.