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Protein

Leu/Ile/Val-binding protein

Gene

livJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids.

GO - Molecular functioni

  • branched-chain amino acid transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • branched-chain amino acid transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:LIVJ-MONOMER.
ECOL316407:JW3425-MONOMER.
MetaCyc:LIVJ-MONOMER.

Protein family/group databases

TCDBi3.A.1.4.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Leu/Ile/Val-binding protein
Short name:
LIV-BP
Gene namesi
Name:livJ
Ordered Locus Names:b3460, JW3425
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10539. livJ.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23233 PublicationsAdd
BLAST
Chaini24 – 367344Leu/Ile/Val-binding proteinPRO_0000017700Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 101

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0AD96.
PaxDbiP0AD96.
PRIDEiP0AD96.

2D gel databases

SWISS-2DPAGEP0AD96.

Interactioni

Protein-protein interaction databases

BioGridi4259328. 18 interactions.
IntActiP0AD96. 4 interactions.
STRINGi511145.b3460.

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 327Combined sources
Beta strandi34 – 363Combined sources
Helixi39 – 5820Combined sources
Turni59 – 613Combined sources
Beta strandi67 – 737Combined sources
Helixi78 – 9013Combined sources
Beta strandi95 – 973Combined sources
Helixi102 – 11514Combined sources
Beta strandi118 – 1236Combined sources
Helixi127 – 1304Combined sources
Beta strandi135 – 1417Combined sources
Helixi144 – 1463Combined sources
Helixi147 – 15711Combined sources
Beta strandi162 – 1687Combined sources
Helixi172 – 18716Combined sources
Beta strandi192 – 1976Combined sources
Helixi206 – 2149Combined sources
Beta strandi219 – 2246Combined sources
Helixi226 – 23813Combined sources
Beta strandi244 – 2474Combined sources
Helixi249 – 2513Combined sources
Helixi254 – 2607Combined sources
Helixi261 – 2644Combined sources
Beta strandi268 – 2714Combined sources
Helixi275 – 2773Combined sources
Helixi279 – 2813Combined sources
Helixi282 – 2909Combined sources
Helixi298 – 31417Combined sources
Helixi320 – 32910Combined sources
Beta strandi332 – 3343Combined sources
Beta strandi337 – 3393Combined sources
Beta strandi347 – 3493Combined sources
Beta strandi353 – 3575Combined sources
Beta strandi363 – 3653Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z15X-ray1.70A24-367[»]
1Z16X-ray1.72A24-367[»]
1Z17X-ray1.96A24-367[»]
1Z18X-ray2.10A24-367[»]
2LIVX-ray2.40A24-367[»]
ProteinModelPortaliP0AD96.
SMRiP0AD96. Positions 24-367.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AD96.

Family & Domainsi

Sequence similaritiesi

Belongs to the leucine-binding protein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CP9. Bacteria.
COG0683. LUCA.
HOGENOMiHOG000130989.
InParanoidiP0AD96.
KOiK01999.
OMAiLWNVARV.
OrthoDBiEOG63587B.
PhylomeDBiP0AD96.

Family and domain databases

InterProiIPR028081. Leu-bd.
IPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR30483:SF16. PTHR30483:SF16. 1 hit.
PfamiPF13458. Peripla_BP_6. 1 hit.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKGKALLA GCIALAFSNM ALAEDIKVAV VGAMSGPVAQ YGDQEFTGAE
60 70 80 90 100
QAVADINAKG GIKGNKLQIV KYDDACDPKQ AVAVANKVVN DGIKYVIGHL
110 120 130 140 150
CSSSTQPASD IYEDEGILMI TPAATAPELT ARGYQLILRT TGLDSDQGPT
160 170 180 190 200
AAKYILEKVK PQRIAIVHDK QQYGEGLARA VQDGLKKGNA NVVFFDGITA
210 220 230 240 250
GEKDFSTLVA RLKKENIDFV YYGGYHPEMG QILRQARAAG LKTQFMGPEG
260 270 280 290 300
VANVSLSNIA GESAEGLLVT KPKNYDQVPA NKPIVDAIKA KKQDPSGAFV
310 320 330 340 350
WTTYAALQSL QAGLNQSDDP AEIAKYLKAN SVDTVMGPLT WDEKGDLKGF
360
EFGVFDWHAN GTATDAK
Length:367
Mass (Da):39,076
Last modified:December 6, 2005 - v1
Checksum:i63F0729C251F18FD
GO

Sequence cautioni

The sequence AAB18435.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31I → T (PubMed:3891753).Curated
Sequence conflicti3 – 31I → T (PubMed:4077929).Curated
Sequence conflicti3 – 31I → T (PubMed:2195019).Curated
Sequence conflicti12 – 121C → L (PubMed:3891753).Curated
Sequence conflicti12 – 121C → L (PubMed:4077929).Curated
Sequence conflicti12 – 121C → L (PubMed:2195019).Curated
Sequence conflicti16 – 161A → G (PubMed:3891753).Curated
Sequence conflicti70 – 701V → A (PubMed:3891753).Curated
Sequence conflicti70 – 701V → A (PubMed:4077929).Curated
Sequence conflicti70 – 701V → A (PubMed:2195019).Curated
Sequence conflicti70 – 701V → A (PubMed:328304).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05516 Genomic DNA. Translation: AAA83881.1.
M29377 Genomic DNA. Translation: AAA24075.1.
U00039 Genomic DNA. Translation: AAB18435.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48185.1.
AP009048 Genomic DNA. Translation: BAE77833.1.
PIRiG65142. BLEC.
RefSeqiYP_026223.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48185; AAT48185; b3460.
BAE77833; BAE77833; BAE77833.
GeneIDi947971.
KEGGiecj:JW3425.
eco:b3460.
PATRICi32122364. VBIEscCol129921_3559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05516 Genomic DNA. Translation: AAA83881.1.
M29377 Genomic DNA. Translation: AAA24075.1.
U00039 Genomic DNA. Translation: AAB18435.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48185.1.
AP009048 Genomic DNA. Translation: BAE77833.1.
PIRiG65142. BLEC.
RefSeqiYP_026223.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z15X-ray1.70A24-367[»]
1Z16X-ray1.72A24-367[»]
1Z17X-ray1.96A24-367[»]
1Z18X-ray2.10A24-367[»]
2LIVX-ray2.40A24-367[»]
ProteinModelPortaliP0AD96.
SMRiP0AD96. Positions 24-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259328. 18 interactions.
IntActiP0AD96. 4 interactions.
STRINGi511145.b3460.

Protein family/group databases

TCDBi3.A.1.4.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AD96.

Proteomic databases

EPDiP0AD96.
PaxDbiP0AD96.
PRIDEiP0AD96.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48185; AAT48185; b3460.
BAE77833; BAE77833; BAE77833.
GeneIDi947971.
KEGGiecj:JW3425.
eco:b3460.
PATRICi32122364. VBIEscCol129921_3559.

Organism-specific databases

EchoBASEiEB0534.
EcoGeneiEG10539. livJ.

Phylogenomic databases

eggNOGiENOG4105CP9. Bacteria.
COG0683. LUCA.
HOGENOMiHOG000130989.
InParanoidiP0AD96.
KOiK01999.
OMAiLWNVARV.
OrthoDBiEOG63587B.
PhylomeDBiP0AD96.

Enzyme and pathway databases

BioCyciEcoCyc:LIVJ-MONOMER.
ECOL316407:JW3425-MONOMER.
MetaCyc:LIVJ-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AD96.
PROiP0AD96.

Family and domain databases

InterProiIPR028081. Leu-bd.
IPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR30483:SF16. PTHR30483:SF16. 1 hit.
PfamiPF13458. Peripla_BP_6. 1 hit.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport."
    Landick R., Oxender D.L.
    J. Biol. Chem. 260:8257-8261(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function."
    Antonucci T.K., Landick R., Oxender D.L.
    J. Cell. Biochem. 29:209-216(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli."
    Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K., Gibson A.L., Oxender D.L.
    J. Biol. Chem. 265:11436-11443(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "The primary structure of a Leu, Ile and Val (LIV)-binding protein from Escherichia coli."
    Ovchinnikov Y.A., Aldanova N.A., Grinkevich V.A., Arzamazova N.M., Moroz I.N.
    FEBS Lett. 78:313-316(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-367.
    Strain: K12.
  8. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-35.
    Strain: K12 / EMG2.
  9. Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F.
    Submitted (FEB-1996) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 24-35.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  10. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  11. "Periplasmic binding protein structure and function. Refined X-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine."
    Sack J.S., Saper M.A., Quiocho F.A.
    J. Mol. Biol. 206:171-191(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiLIVJ_ECOLI
AccessioniPrimary (citable) accession number: P0AD96
Secondary accession number(s): P02917
, P76698, Q2M7C3, Q8CVL7, Q8X6S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 6, 2005
Last modified: March 16, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.