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Protein

D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH

Gene

ampH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the cross-linked dimers tetrapentapeptide (D45) and tetratetrapeptide (D44). Removes the terminal D-alanine from muropeptides and disaccharide pentapeptide M5 with a C-terminal D-Ala-D-Ala dipeptide. Associated with recycling and remodeling of peptidoglycan (PG). Also displays a low beta-lactamase activity.1 Publication

Enzyme regulationi

Inhibited by cefmetazole.1 Publication

Kineticsi

  1. KM=102 µM for D45 (at 37 degrees Celsius and pH 7.3)1 Publication
  2. KM=134 µM for D44 (at 37 degrees Celsius and pH 7.3)1 Publication
  3. KM=225 µM for M5 (at 37 degrees Celsius and pH 7.5)1 Publication
  1. Vmax=4.98 nmol/min/µg enzyme with M5 as substrate (at 37 degrees Celsius and pH 7.5)1 Publication
  2. Vmax=162 nmol/min/µg enzyme with D44 as substrate (at 37 degrees Celsius and pH 7.3)1 Publication
  3. Vmax=174 nmol/min/µg enzyme with D45 as substrate (at 37 degrees Celsius and pH 7.3)1 Publication

GO - Molecular functioni

  • carboxypeptidase activity Source: UniProtKB
  • endopeptidase activity Source: UniProtKB
  • penicillin binding Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan catabolic process Source: UniProtKB
  • regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG12867-MONOMER.
ECOL316407:JW5052-MONOMER.
MetaCyc:EG12867-MONOMER.

Protein family/group databases

MEROPSiS12.012.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH (EC:3.4.-.-)
Alternative name(s):
DD-alanine-endopeptidase
DD-carboxypeptidase
Penicillin-binding protein AmpH
Gene namesi
Name:ampH
Synonyms:yaiH
Ordered Locus Names:b0376, JW5052
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12867. ampH.

Subcellular locationi

  • Cell inner membrane 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption is viable and exhibits no overt growth defects, but produces morphologically aberrant cells, particularly in cell filaments induced by aztreonam.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 385364D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpHPRO_0000195474Add
BLAST

Proteomic databases

PaxDbiP0AD70.
PRIDEiP0AD70.

Interactioni

Protein-protein interaction databases

BioGridi4259482. 219 interactions.
DIPiDIP-47910N.
IntActiP0AD70. 1 interaction.
STRINGi511145.b0376.

Structurei

3D structure databases

ProteinModelPortaliP0AD70.
SMRiP0AD70. Positions 30-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107R6D. Bacteria.
COG1680. LUCA.
HOGENOMiHOG000117477.
InParanoidiP0AD70.
KOiK18988.
OMAiPASANAY.
PhylomeDBiP0AD70.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSLLFSAV LCAASLTSVH AAQPITEPEF ASDIVDRYAD HIFYGSGATG
60 70 80 90 100
MALVVIDGNQ RVFRSYGETR PGNNVRPQLD SVVRIASLTK LMTSEMLVKL
110 120 130 140 150
LDQGTVKLND PLSKYAPPGA RVPTYNGTPI TLVNLATHTS ALPREQPGGA
160 170 180 190 200
AHRPVFVWPT REQRWKYLST AKLKAAPGSQ AAYSNLAFDL LADALANASG
210 220 230 240 250
KPYTQLFEEQ ITRPLGMKDT TYTPSPDQCR RLMVAERGAS PCNNTLAAIG
260 270 280 290 300
SGGVYSTPGD MMRWMQQYLS SDFYQRSNQA DRMQTLIYQR AQFTKVIGMD
310 320 330 340 350
VPGKADALGL GWVYMAPKEG RPGIIQKTGG GGGFITYMAM IPQKNIGAFV
360 370 380
VVTRSPLTRF KNMSDGINDL VTELSGNKPL VIPAS
Length:385
Mass (Da):41,849
Last modified:December 6, 2005 - v1
Checksum:i2D249CBA78022947
GO

Sequence cautioni

The sequence AAB18099 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Mass spectrometryi

Molecular mass is 41860 Da from positions 23 - 385. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18099.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73479.1.
AP009048 Genomic DNA. Translation: BAE76157.1.
X54153 Genomic DNA. No translation available.
PIRiH64765.
RefSeqiNP_414910.1. NC_000913.3.
WP_000830741.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73479; AAC73479; b0376.
BAE76157; BAE76157; BAE76157.
GeneIDi946904.
KEGGiecj:JW5052.
eco:b0376.
PATRICi32115891. VBIEscCol129921_0387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18099.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73479.1.
AP009048 Genomic DNA. Translation: BAE76157.1.
X54153 Genomic DNA. No translation available.
PIRiH64765.
RefSeqiNP_414910.1. NC_000913.3.
WP_000830741.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AD70.
SMRiP0AD70. Positions 30-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259482. 219 interactions.
DIPiDIP-47910N.
IntActiP0AD70. 1 interaction.
STRINGi511145.b0376.

Protein family/group databases

MEROPSiS12.012.

Proteomic databases

PaxDbiP0AD70.
PRIDEiP0AD70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73479; AAC73479; b0376.
BAE76157; BAE76157; BAE76157.
GeneIDi946904.
KEGGiecj:JW5052.
eco:b0376.
PATRICi32115891. VBIEscCol129921_0387.

Organism-specific databases

EchoBASEiEB2708.
EcoGeneiEG12867. ampH.

Phylogenomic databases

eggNOGiENOG4107R6D. Bacteria.
COG1680. LUCA.
HOGENOMiHOG000117477.
InParanoidiP0AD70.
KOiK18988.
OMAiPASANAY.
PhylomeDBiP0AD70.

Enzyme and pathway databases

BioCyciEcoCyc:EG12867-MONOMER.
ECOL316407:JW5052-MONOMER.
MetaCyc:EG12867-MONOMER.

Miscellaneous databases

PROiP0AD70.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMPH_ECOLI
AccessioniPrimary (citable) accession number: P0AD70
Secondary accession number(s): P46127, P75701, Q2MC49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.