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Protein

D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH

Gene

ampH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes the cross-linked dimers tetrapentapeptide (D45) and tetratetrapeptide (D44). Removes the terminal D-alanine from muropeptides and disaccharide pentapeptide M5 with a C-terminal D-Ala-D-Ala dipeptide. Associated with recycling and remodeling of peptidoglycan (PG). Also displays a low beta-lactamase activity.1 Publication

Enzyme regulationi

Inhibited by cefmetazole.1 Publication

Kineticsi

  1. KM=102 µM for D45 (at 37 degrees Celsius and pH 7.3)1 Publication
  2. KM=134 µM for D44 (at 37 degrees Celsius and pH 7.3)1 Publication
  3. KM=225 µM for M5 (at 37 degrees Celsius and pH 7.5)1 Publication
  1. Vmax=4.98 nmol/min/µg enzyme with M5 as substrate (at 37 degrees Celsius and pH 7.5)1 Publication
  2. Vmax=162 nmol/min/µg enzyme with D44 as substrate (at 37 degrees Celsius and pH 7.3)1 Publication
  3. Vmax=174 nmol/min/µg enzyme with D45 as substrate (at 37 degrees Celsius and pH 7.3)1 Publication

GO - Molecular functioni

  • carboxypeptidase activity Source: UniProtKB
  • endopeptidase activity Source: UniProtKB
  • penicillin binding Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan catabolic process Source: UniProtKB
  • regulation of cell shape Source: UniProtKB

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell shape, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG12867-MONOMER
MetaCyc:EG12867-MONOMER

Protein family/group databases

MEROPSiS12.012

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH (EC:3.4.-.-)
Alternative name(s):
DD-alanine-endopeptidase
DD-carboxypeptidase
Penicillin-binding protein AmpH
Gene namesi
Name:ampH
Synonyms:yaiH
Ordered Locus Names:b0376, JW5052
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12867 ampH

Subcellular locationi

  • Cell inner membrane 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption is viable and exhibits no overt growth defects, but produces morphologically aberrant cells, particularly in cell filaments induced by aztreonam.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000019547422 – 385D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpHAdd BLAST364

Proteomic databases

PaxDbiP0AD70
PRIDEiP0AD70

Interactioni

Protein-protein interaction databases

BioGridi4259482, 246 interactors
DIPiDIP-47910N
IntActiP0AD70, 1 interactor
STRINGi316385.ECDH10B_0333

Structurei

3D structure databases

ProteinModelPortaliP0AD70
SMRiP0AD70
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107R6D Bacteria
COG1680 LUCA
HOGENOMiHOG000117477
InParanoidiP0AD70
KOiK18988
OMAiTTKYFLA
PhylomeDBiP0AD70

Family and domain databases

InterProiView protein in InterPro
IPR001466 Beta-lactam-related
IPR012338 Beta-lactam/transpept-like
PfamiView protein in Pfam
PF00144 Beta-lactamase, 1 hit
SUPFAMiSSF56601 SSF56601, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSLLFSAV LCAASLTSVH AAQPITEPEF ASDIVDRYAD HIFYGSGATG
60 70 80 90 100
MALVVIDGNQ RVFRSYGETR PGNNVRPQLD SVVRIASLTK LMTSEMLVKL
110 120 130 140 150
LDQGTVKLND PLSKYAPPGA RVPTYNGTPI TLVNLATHTS ALPREQPGGA
160 170 180 190 200
AHRPVFVWPT REQRWKYLST AKLKAAPGSQ AAYSNLAFDL LADALANASG
210 220 230 240 250
KPYTQLFEEQ ITRPLGMKDT TYTPSPDQCR RLMVAERGAS PCNNTLAAIG
260 270 280 290 300
SGGVYSTPGD MMRWMQQYLS SDFYQRSNQA DRMQTLIYQR AQFTKVIGMD
310 320 330 340 350
VPGKADALGL GWVYMAPKEG RPGIIQKTGG GGGFITYMAM IPQKNIGAFV
360 370 380
VVTRSPLTRF KNMSDGINDL VTELSGNKPL VIPAS
Length:385
Mass (Da):41,849
Last modified:December 6, 2005 - v1
Checksum:i2D249CBA78022947
GO

Sequence cautioni

The sequence AAB18099 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Mass spectrometryi

Molecular mass is 41860 Da from positions 23 - 385. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18099.1 Different initiation.
U00096 Genomic DNA Translation: AAC73479.1
AP009048 Genomic DNA Translation: BAE76157.1
X54153 Genomic DNA No translation available.
PIRiH64765
RefSeqiNP_414910.1, NC_000913.3
WP_000830741.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73479; AAC73479; b0376
BAE76157; BAE76157; BAE76157
GeneIDi946904
KEGGiecj:JW5052
eco:b0376
PATRICifig|1411691.4.peg.1903

Similar proteinsi

Entry informationi

Entry nameiAMPH_ECOLI
AccessioniPrimary (citable) accession number: P0AD70
Secondary accession number(s): P46127, P75701, Q2MC49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health