P0AD70 (AMPH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Penicillin-binding protein AmpH

Gene names

Name:	ampH
Synonyms:	yaiH
Ordered Locus Names:	b0376, JW5052

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds penicillin and contributes to the normal morphology of E.coli.
Sequence similarities	Belongs to the beta-lactamase family.
Sequence caution	The sequence AAB18099.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to drug Inferred from direct assay Ref.6. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Penicillin-binding protein AmpH		PRO_0000195474

Sequences

	Sequence		Length	Mass (Da)
	P0AD70 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 2D249CBA78022947	FASTA	385	41,849
	10 20 30 40 50 60 MKRSLLFSAV LCAASLTSVH AAQPITEPEF ASDIVDRYAD HIFYGSGATG MALVVIDGNQ 70 80 90 100 110 120 RVFRSYGETR PGNNVRPQLD SVVRIASLTK LMTSEMLVKL LDQGTVKLND PLSKYAPPGA 130 140 150 160 170 180 RVPTYNGTPI TLVNLATHTS ALPREQPGGA AHRPVFVWPT REQRWKYLST AKLKAAPGSQ 190 200 210 220 230 240 AAYSNLAFDL LADALANASG KPYTQLFEEQ ITRPLGMKDT TYTPSPDQCR RLMVAERGAS 250 260 270 280 290 300 PCNNTLAAIG SGGVYSTPGD MMRWMQQYLS SDFYQRSNQA DRMQTLIYQR AQFTKVIGMD 310 320 330 340 350 360 VPGKADALGL GWVYMAPKEG RPGIIQKTGG GGGFITYMAM IPQKNIGAFV VVTRSPLTRF 370 380 KNMSDGINDL VTELSGNKPL VIPAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Nucleotide sequence of the Escherichia coli regulatory gene mprA and construction and characterization of mprA-deficient mutants." del Castillo I., Gonzalez-Pastor J.E., San Millan J.L., Moreno F. J. Bacteriol. 173:3924-3929(1991) [PubMed: 1840583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-78. Strain: K12.
[5]	"Detection of new genes in a bacterial genome using Markov models for three gene classes." Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A. Nucleic Acids Res. 23:3554-3562(1995) [PubMed: 7567469] [Abstract] Cited for: IDENTIFICATION.
[6]	"AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli." Henderson T.A., Young K.D., Denome S.A., Elf P.K. J. Bacteriol. 179:6112-6121(1997) [PubMed: 9324260] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18099.1. Different initiation. U00096 Genomic DNA. Translation: AAC73479.1. AP009048 Genomic DNA. Translation: BAE76157.1. X54153 Genomic DNA. No translation available.
PIR	H64765.
RefSeq	NP_414910.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AD70.
SMR	P0AD70. Positions 30-374.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47910N.
Protein family/group databases
MEROPS	S12.A02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000029; EBESCP00000000029; EBESCG00000000023. EBESCT00000016362; EBESCP00000015653; EBESCG00000015422.
GeneID	946904.
GenomeReviews	Gene locus JW5052 in contig AP009048_GR. Gene locus b0376 in contig U00096_GR.
KEGG	ecj:JW5052. eco:b0376.
PATRIC	32115891. VBIEscCol129921_0387.
Organism-specific databases
EchoBASE	EB2708.
EcoGene	EG12867. ampH.
Phylogenomic databases
eggNOG	COG1680.
GeneTree	EBGT00050000010224.
HOGENOM	HBG416564.
OMA	GQRYEDY.
PhylomeDB	P0AD70.
ProtClustDB	PRK10662.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12867-MONOMER. MetaCyc:EG12867-MONOMER.
Gene expression databases
Genevestigator	P0AD70.
Family and domain databases
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. [Graphical view]
Gene3D	G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMPH_ECOLI

Accession

Primary (citable) accession number: P0AD70
Secondary accession number(s): P46127, P75701, Q2MC49

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program