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P0AD69 (FTSI_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidoglycan synthase FtsI

EC=2.4.1.129
Alternative name(s):
Penicillin-binding protein 3
Short name=PBP-3
Peptidoglycan glycosyltransferase 3
Gene names
Name:ftsI
Synonyms:pbpB
Ordered Locus Names:Z0094, ECs0088
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates By similarity.

Catalytic activity

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell inner membrane; Single-pass membrane protein; Periplasmic side By similarity. Note: The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein By similarity.

Domain

Has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits) By similarity.

Sequence similarities

Belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Peptidoglycan synthase FtsI
PRO_0000043363
Propeptide578 – 58811 By similarity
PRO_0000043364

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 3921Helical; Potential
Topological domain40 – 577538Periplasmic Potential

Sites

Active site3071Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AD69 [UniParc].

Last modified March 20, 1987. Version 1.
Checksum: C89A403D5980B2CD

FASTA58863,877
        10         20         30         40         50         60 
MKAAAKTQKP KRQEEHANFI SWRFALLCGC ILLALAFLLG RVAWLQVISP DMLVKEGDMR 

        70         80         90        100        110        120 
SLRVQQVSTS RGMITDRSGR PLAVSVPVKA IWADPKEVHD AGGISVGDRW KALANALNIP 

       130        140        150        160        170        180 
LDQLSARINA NPKGRFIYLA RQVNPDMADY IKKLKLPGIH LREESRRYYP SGEVTAHLIG 

       190        200        210        220        230        240 
FTNVDSQGIE GVEKSFDKWL TGQPGERIVR KDRYGRVIED ISSTDSQAAH NLALSIDERL 

       250        260        270        280        290        300 
QALVYRELNN AVAFNKAESG SAVLVDVNTG EVLAMANSPS YNPNNLSGTP KEAMRNRTIT 

       310        320        330        340        350        360 
DVFEPGSTVK PMVVMTALQR GVVRENSVLN TIPYRINGHE IKDVARYSEL TLTGVLQKSS 

       370        380        390        400        410        420 
NVGVSKLALA MPSSALVDTY SRFGLGKATN LGLVGERSGL YPQKQRWSDI ERATFSFGYG 

       430        440        450        460        470        480 
LMVTPLQLAR VYATIGSYGI YRPLSITKVD PPVPGERVFP ESIVRTVVHM MESVALPGGG 

       490        500        510        520        530        540 
GVKAAIKGYR IAIKTGTAKK VGPDGRYINK YIAYTAGVAP ASQPRFALVV VINDPQAGKY 

       550        560        570        580 
YGGAVSAPVF GAIMGGVLRT MNIEPDALTT GDKNEFVINQ GEGTGGRS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG54388.1.
BA000007 Genomic DNA. Translation: BAB33511.1.
PIRH85490.
H90639.
RefSeqNP_285780.1. NC_002655.2.
NP_308115.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0AD69.
SMRP0AD69. Positions 60-570.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1031936.
STRING155864.Z0094.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG54388; AAG54388; Z0094.
BAB33511; BAB33511; BAB33511.
GeneID913535.
956770.
KEGGece:Z0094.
ecs:ECs0088.
PATRIC18349118. VBIEscCol44059_0089.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0768.
HOGENOMHOG000049554.
KOK03587.
OMAHPRYFNF.
OrthoDBEOG6N0HHV.

Enzyme and pathway databases

BioCycECOL386585:GJFA-86-MONOMER.
ECOO157:FTSI-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFTSI_ECO57
AccessionPrimary (citable) accession number: P0AD69
Secondary accession number(s): P04286
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways