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P0AD69

- FTSI_ECO57

UniProt

P0AD69 - FTSI_ECO57

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Protein

Peptidoglycan synthase FtsI

Gene

ftsI

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates (By similarity).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei307 – 3071Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro
  2. peptidoglycan glycosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  5. regulation of cell shape Source: UniProtKB-KW
  6. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciECOL386585:GJFA-86-MONOMER.
ECOO157:FTSI-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan synthase FtsI (EC:2.4.1.129)
Alternative name(s):
Penicillin-binding protein 3
Short name:
PBP-3
Peptidoglycan glycosyltransferase 3
Gene namesi
Name:ftsI
Synonyms:pbpB
Ordered Locus Names:Z0094, ECs0088
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cell inner membrane By similarity; Single-pass membrane protein By similarity; Periplasmic side By similarity
Note: The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3921HelicalSequence AnalysisAdd
BLAST
Topological domaini40 – 577538PeriplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Peptidoglycan synthase FtsIPRO_0000043363Add
BLAST
Propeptidei578 – 58811By similarityPRO_0000043364Add
BLAST

Interactioni

Protein-protein interaction databases

MINTiMINT-1031936.
STRINGi155864.Z0094.

Structurei

3D structure databases

ProteinModelPortaliP0AD69.
SMRiP0AD69. Positions 71-567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).By similarity

Sequence similaritiesi

Belongs to the transpeptidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0768.
HOGENOMiHOG000049554.
KOiK03587.
OMAiHPRYFNF.
OrthoDBiEOG6N0HHV.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD69-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAAAKTQKP KRQEEHANFI SWRFALLCGC ILLALAFLLG RVAWLQVISP
60 70 80 90 100
DMLVKEGDMR SLRVQQVSTS RGMITDRSGR PLAVSVPVKA IWADPKEVHD
110 120 130 140 150
AGGISVGDRW KALANALNIP LDQLSARINA NPKGRFIYLA RQVNPDMADY
160 170 180 190 200
IKKLKLPGIH LREESRRYYP SGEVTAHLIG FTNVDSQGIE GVEKSFDKWL
210 220 230 240 250
TGQPGERIVR KDRYGRVIED ISSTDSQAAH NLALSIDERL QALVYRELNN
260 270 280 290 300
AVAFNKAESG SAVLVDVNTG EVLAMANSPS YNPNNLSGTP KEAMRNRTIT
310 320 330 340 350
DVFEPGSTVK PMVVMTALQR GVVRENSVLN TIPYRINGHE IKDVARYSEL
360 370 380 390 400
TLTGVLQKSS NVGVSKLALA MPSSALVDTY SRFGLGKATN LGLVGERSGL
410 420 430 440 450
YPQKQRWSDI ERATFSFGYG LMVTPLQLAR VYATIGSYGI YRPLSITKVD
460 470 480 490 500
PPVPGERVFP ESIVRTVVHM MESVALPGGG GVKAAIKGYR IAIKTGTAKK
510 520 530 540 550
VGPDGRYINK YIAYTAGVAP ASQPRFALVV VINDPQAGKY YGGAVSAPVF
560 570 580
GAIMGGVLRT MNIEPDALTT GDKNEFVINQ GEGTGGRS
Length:588
Mass (Da):63,877
Last modified:March 20, 1987 - v1
Checksum:iC89A403D5980B2CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54388.1.
BA000007 Genomic DNA. Translation: BAB33511.1.
PIRiH85490.
H90639.
RefSeqiNP_285780.1. NC_002655.2.
NP_308115.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54388; AAG54388; Z0094.
BAB33511; BAB33511; BAB33511.
GeneIDi913535.
956770.
KEGGiece:Z0094.
ecs:ECs0088.
PATRICi18349118. VBIEscCol44059_0089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54388.1 .
BA000007 Genomic DNA. Translation: BAB33511.1 .
PIRi H85490.
H90639.
RefSeqi NP_285780.1. NC_002655.2.
NP_308115.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0AD69.
SMRi P0AD69. Positions 71-567.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1031936.
STRINGi 155864.Z0094.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG54388 ; AAG54388 ; Z0094 .
BAB33511 ; BAB33511 ; BAB33511 .
GeneIDi 913535.
956770.
KEGGi ece:Z0094.
ecs:ECs0088.
PATRICi 18349118. VBIEscCol44059_0089.

Phylogenomic databases

eggNOGi COG0768.
HOGENOMi HOG000049554.
KOi K03587.
OMAi HPRYFNF.
OrthoDBi EOG6N0HHV.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci ECOL386585:GJFA-86-MONOMER.
ECOO157:FTSI-MONOMER.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiFTSI_ECO57
AccessioniPrimary (citable) accession number: P0AD69
Secondary accession number(s): P04286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3