Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peptidoglycan D,D-transpeptidase FtsI

Gene

ftsI

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes cross-linking of the peptidoglycan cell wall at the division septum.UniRule annotation

Catalytic activityi

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei307Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Septation

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase FtsIUniRule annotation (EC:3.4.16.4UniRule annotation)
Alternative name(s):
Penicillin-binding protein 3UniRule annotation
Short name:
PBP-3UniRule annotation
Gene namesi
Name:ftsIUniRule annotation
Synonyms:pbpB
Ordered Locus Names:Z0094, ECs0088
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei19 – 39HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB05659. faropenem medoxomil.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000433631 – 577Peptidoglycan D,D-transpeptidase FtsIAdd BLAST577
PropeptideiPRO_0000043364578 – 588By similarityAdd BLAST11

Interactioni

Protein-protein interaction databases

MINTiMINT-1031936.
STRINGi155864.Z0094.

Structurei

3D structure databases

ProteinModelPortaliP0AD69.
SMRiP0AD69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transpeptidase family. FtsI subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
HOGENOMiHOG000049554.
KOiK03587.
OMAiSIEMDSC.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_02080. FtsI_transpept. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
PfamiView protein in Pfam
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAAAKTQKP KRQEEHANFI SWRFALLCGC ILLALAFLLG RVAWLQVISP
60 70 80 90 100
DMLVKEGDMR SLRVQQVSTS RGMITDRSGR PLAVSVPVKA IWADPKEVHD
110 120 130 140 150
AGGISVGDRW KALANALNIP LDQLSARINA NPKGRFIYLA RQVNPDMADY
160 170 180 190 200
IKKLKLPGIH LREESRRYYP SGEVTAHLIG FTNVDSQGIE GVEKSFDKWL
210 220 230 240 250
TGQPGERIVR KDRYGRVIED ISSTDSQAAH NLALSIDERL QALVYRELNN
260 270 280 290 300
AVAFNKAESG SAVLVDVNTG EVLAMANSPS YNPNNLSGTP KEAMRNRTIT
310 320 330 340 350
DVFEPGSTVK PMVVMTALQR GVVRENSVLN TIPYRINGHE IKDVARYSEL
360 370 380 390 400
TLTGVLQKSS NVGVSKLALA MPSSALVDTY SRFGLGKATN LGLVGERSGL
410 420 430 440 450
YPQKQRWSDI ERATFSFGYG LMVTPLQLAR VYATIGSYGI YRPLSITKVD
460 470 480 490 500
PPVPGERVFP ESIVRTVVHM MESVALPGGG GVKAAIKGYR IAIKTGTAKK
510 520 530 540 550
VGPDGRYINK YIAYTAGVAP ASQPRFALVV VINDPQAGKY YGGAVSAPVF
560 570 580
GAIMGGVLRT MNIEPDALTT GDKNEFVINQ GEGTGGRS
Length:588
Mass (Da):63,877
Last modified:March 20, 1987 - v1
Checksum:iC89A403D5980B2CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54388.1.
BA000007 Genomic DNA. Translation: BAB33511.1.
PIRiH85490.
H90639.
RefSeqiNP_308115.1. NC_002695.1.
WP_000642196.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG54388; AAG54388; Z0094.
BAB33511; BAB33511; BAB33511.
GeneIDi913535.
KEGGiece:Z0094.
ecs:ECs0088.
PATRICi18349118. VBIEscCol44059_0089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54388.1.
BA000007 Genomic DNA. Translation: BAB33511.1.
PIRiH85490.
H90639.
RefSeqiNP_308115.1. NC_002695.1.
WP_000642196.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0AD69.
SMRiP0AD69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1031936.
STRINGi155864.Z0094.

Chemistry databases

DrugBankiDB05659. faropenem medoxomil.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54388; AAG54388; Z0094.
BAB33511; BAB33511; BAB33511.
GeneIDi913535.
KEGGiece:Z0094.
ecs:ECs0088.
PATRICi18349118. VBIEscCol44059_0089.

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
HOGENOMiHOG000049554.
KOiK03587.
OMAiSIEMDSC.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_02080. FtsI_transpept. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
PfamiView protein in Pfam
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFTSI_ECO57
AccessioniPrimary (citable) accession number: P0AD69
Secondary accession number(s): P04286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: March 15, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.