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Protein

Peptidoglycan D,D-transpeptidase FtsI

Gene

ftsI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum (PubMed:1103132, PubMed:6450748, PubMed:9614966, PubMed:3531167, PubMed:7030331). Required for localization of FtsN (PubMed:9282742).6 Publications

Caution

Was originally thought to be a bifunctional enzyme with transglycosylase and transpeptidase activities.1 Publication

Catalytic activityi

Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation3 Publications

Enzyme regulationi

Inhibited by beta-lactam antibiotics such as penicillin, moenomycin, macarbomycin, furazlocillin and piperacillin. Antibiotics inhibit the activity by binding to the catalytic serine.3 Publications

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei307Acyl-ester intermediateUniRule annotation1 Publication1

GO - Molecular functioni

  • penicillin binding Source: EcoliWiki
  • peptidoglycan glycosyltransferase activity Source: EcoliWiki
  • serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: EcoliWiki
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  • regulation of cell shape Source: UniProtKB-KW
  • response to drug Source: EcoliWiki

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Septation

Enzyme and pathway databases

BioCyciEcoCyc:EG10341-MONOMER
MetaCyc:EG10341-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase FtsIUniRule annotationCurated (EC:3.4.16.4UniRule annotation3 Publications)
Alternative name(s):
Essential cell division protein FtsICurated
Murein transpeptidase1 Publication
Penicillin-binding protein 32 PublicationsUniRule annotation
Short name:
PBP-31 PublicationUniRule annotation
Peptidoglycan synthase FtsICurated
Gene namesi
Name:ftsI1 PublicationUniRule annotation
Synonyms:pbpB
Ordered Locus Names:b0084, JW0082
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10341 ftsI

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18Cytoplasmic1 PublicationAdd BLAST18
Transmembranei19 – 39HelicalUniRule annotationAdd BLAST21
Topological domaini40 – 577Periplasmic1 PublicationAdd BLAST538

GO - Cellular componenti

  • cell division site Source: EcoliWiki
  • integral component of plasma membrane Source: EcoliWiki
  • intrinsic component of plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23R → C or H: Impairs septal localization. 1 Publication1
Mutagenesisi39L → P: Impairs septal localization. 1 Publication1
Mutagenesisi46Q → H: Impairs septal localization. 1 Publication1
Mutagenesisi57G → D: Impairs recruitment of FtsN to the septal ring. 1 Publication1
Mutagenesisi61S → F or P: Impairs recruitment of FtsN to the septal ring. 1 Publication1
Mutagenesisi62L → P: Impairs recruitment of FtsN to the septal ring. 1 Publication1
Mutagenesisi210R → C or H: Impairs recruitment of FtsN to the septal ring. 1 Publication1
Mutagenesisi307S → A or T: Unable to bind penicillin. 1 Publication1
Mutagenesisi307S → C: Still able to bind penicillin. 1 Publication1
Mutagenesisi361N → S in PBPBR1; obtained after selection for increased resistance to cephalexin, causes a change in the shape of the cell: The polar caps are pointed. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2354204
DrugBankiDB01327 Cefazolin
DB01413 Cefepime
DB00267 Cefmenoxime
DB00274 Cefmetazole
DB01328 Cefonicid
DB01329 Cefoperazone
DB01331 Cefoxitin
DB00430 Cefpiramide
DB01416 Cefpodoxime
DB00438 Ceftazidime
DB01415 Ceftibuten
DB01332 Ceftizoxime
DB04918 Ceftobiprole
DB00303 Ertapenem

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000170521 – 577Peptidoglycan D,D-transpeptidase FtsIAdd BLAST577
PropeptideiPRO_0000017053578 – 5881 PublicationAdd BLAST11

Proteomic databases

PaxDbiP0AD68
PRIDEiP0AD68

Interactioni

Subunit structurei

Homodimer (PubMed:20847002). Forms a complex with FtsW (PubMed:20847002). Interacts with FtsQ (PubMed:17185541).2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4261637, 166 interactors
DIPiDIP-47950N
IntActiP0AD68, 18 interactors
MINTiP0AD68
STRINGi316385.ECDH10B_0066

Structurei

3D structure databases

ProteinModelPortaliP0AD68
SMRiP0AD68
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Contains an N-terminal membrane anchor-containing module, a central non-catalytic domain and a C-terminal penicillin-binding (PB) catalytic domain. The transmembrane region is essential for localization to the septal ring, interaction with FtsW and cell septation.4 Publications

Sequence similaritiesi

Belongs to the transpeptidase family. FtsI subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN Bacteria
COG0768 LUCA
HOGENOMiHOG000049554
InParanoidiP0AD68
KOiK03587
OMAiNVGMIKI
PhylomeDBiP0AD68

Family and domain databases

HAMAPiMF_02080 FtsI_transpept, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR037532 FtsI_transpept
IPR005311 PBP_dimer
IPR036138 PBP_dimer_sf
IPR001460 PCN-bd_Tpept
PfamiView protein in Pfam
PF03717 PBP_dimer, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF56519 SSF56519, 1 hit
SSF56601 SSF56601, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAAAKTQKP KRQEEHANFI SWRFALLCGC ILLALAFLLG RVAWLQVISP
60 70 80 90 100
DMLVKEGDMR SLRVQQVSTS RGMITDRSGR PLAVSVPVKA IWADPKEVHD
110 120 130 140 150
AGGISVGDRW KALANALNIP LDQLSARINA NPKGRFIYLA RQVNPDMADY
160 170 180 190 200
IKKLKLPGIH LREESRRYYP SGEVTAHLIG FTNVDSQGIE GVEKSFDKWL
210 220 230 240 250
TGQPGERIVR KDRYGRVIED ISSTDSQAAH NLALSIDERL QALVYRELNN
260 270 280 290 300
AVAFNKAESG SAVLVDVNTG EVLAMANSPS YNPNNLSGTP KEAMRNRTIT
310 320 330 340 350
DVFEPGSTVK PMVVMTALQR GVVRENSVLN TIPYRINGHE IKDVARYSEL
360 370 380 390 400
TLTGVLQKSS NVGVSKLALA MPSSALVDTY SRFGLGKATN LGLVGERSGL
410 420 430 440 450
YPQKQRWSDI ERATFSFGYG LMVTPLQLAR VYATIGSYGI YRPLSITKVD
460 470 480 490 500
PPVPGERVFP ESIVRTVVHM MESVALPGGG GVKAAIKGYR IAIKTGTAKK
510 520 530 540 550
VGPDGRYINK YIAYTAGVAP ASQPRFALVV VINDPQAGKY YGGAVSAPVF
560 570 580
GAIMGGVLRT MNIEPDALTT GDKNEFVINQ GEGTGGRS
Length:588
Mass (Da):63,877
Last modified:March 20, 1987 - v1
Checksum:iC89A403D5980B2CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00137 Genomic DNA Translation: AAA24300.1
X55034 Genomic DNA Translation: CAA38861.1
U00096 Genomic DNA Translation: AAC73195.1
AP009048 Genomic DNA Translation: BAB96652.1
S49802 Genomic DNA Translation: AAB24312.1
S49875 Genomic DNA Translation: AAB24310.1
X55814 Genomic DNA Translation: CAA39333.1
PIRiA93123 ZPECP3
RefSeqiNP_414626.1, NC_000913.3
WP_000642196.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73195; AAC73195; b0084
BAB96652; BAB96652; BAB96652
GeneIDi944799
KEGGiecj:JW0082
eco:b0084
PATRICifig|1411691.4.peg.2196

Similar proteinsi

Entry informationi

Entry nameiFTSI_ECOLI
AccessioniPrimary (citable) accession number: P0AD68
Secondary accession number(s): P04286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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