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P0AD65

- PBP2_ECOLI

UniProt

P0AD65 - PBP2_ECOLI

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Protein
Penicillin-binding protein 2
Gene
mrdA, pbpA, b0635, JW0630
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross-linked peptidoglycan from the lipid intermediates.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei330 – 3301Acyl-ester intermediate

GO - Molecular functioni

  1. drug binding Source: EcoCyc
  2. penicillin binding Source: EcoliWiki
  3. peptidoglycan L,D-transpeptidase activity Source: EcoliWiki
  4. serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc

GO - Biological processi

  1. cell wall organization Source: EcoCyc
  2. peptidoglycan biosynthetic process Source: EcoCyc
  3. proteolysis Source: GOC
  4. regulation of cell shape Source: EcoCyc
  5. response to antibiotic Source: EcoliWiki
  6. response to drug Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10606-MONOMER.
ECOL316407:JW0630-MONOMER.
MetaCyc:EG10606-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2
Short name:
PBP-2
Gene namesi
Name:mrdA
Synonyms:pbpA
Ordered Locus Names:b0635, JW0630
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10606. mrdA.

Subcellular locationi

GO - Cellular componenti

  1. integral component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Penicillin-binding protein 2
PRO_0000195444Add
BLAST

Proteomic databases

PaxDbiP0AD65.
PRIDEiP0AD65.

Expressioni

Gene expression databases

GenevestigatoriP0AD65.

Interactioni

Protein-protein interaction databases

DIPiDIP-48190N.
IntActiP0AD65. 2 interactions.
STRINGi511145.b0635.

Structurei

3D structure databases

ProteinModelPortaliP0AD65.
SMRiP0AD65. Positions 56-614.

Family & Domainsi

Domaini

Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Sequence similaritiesi

Belongs to the transpeptidase family.

Phylogenomic databases

eggNOGiCOG0768.
HOGENOMiHOG000266120.
KOiK05515.
OMAiNDGQVKT.
OrthoDBiEOG6N0HHV.
PhylomeDBiP0AD65.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
[Graphical view]
PfamiPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AD65-1 [UniParc]FASTAAdd to Basket

« Hide

MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY    50
QTRSNENRIK LVPIAPSRGI IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ 100
TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE VQVARFAVNQ 150
YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA 200
ATHDIGKLGI ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI 250
YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT GGVLALVSTP SYDPNLFVDG 300
ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT 350
TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG 400
IDRLSEWMGK FGYGHYTGID LAEERSGNMP TREWKQKRFK KPWYQGDTIP 450
VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED GKQVPWVQPH 500
EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV 550
FGLKANETYN AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV 600
GTLMRQILDH IMLGDNNTDL PAENPAVAAA EDH 633
Length:633
Mass (Da):70,857
Last modified:August 1, 1988 - v1
Checksum:iFE2305C002743AF6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04516 Genomic DNA. Translation: CAA28201.1.
U82598 Genomic DNA. Translation: AAB40835.1.
U00096 Genomic DNA. Translation: AAC73736.1.
AP009048 Genomic DNA. Translation: BAA35282.1.
M22857 Genomic DNA. Translation: AAA24570.1.
PIRiC24995. ZPECP2.
RefSeqiNP_415168.1. NC_000913.3.
YP_488926.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73736; AAC73736; b0635.
BAA35282; BAA35282; BAA35282.
GeneIDi12930918.
945240.
KEGGiecj:Y75_p0625.
eco:b0635.
PATRICi32116453. VBIEscCol129921_0666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04516 Genomic DNA. Translation: CAA28201.1 .
U82598 Genomic DNA. Translation: AAB40835.1 .
U00096 Genomic DNA. Translation: AAC73736.1 .
AP009048 Genomic DNA. Translation: BAA35282.1 .
M22857 Genomic DNA. Translation: AAA24570.1 .
PIRi C24995. ZPECP2.
RefSeqi NP_415168.1. NC_000913.3.
YP_488926.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AD65.
SMRi P0AD65. Positions 56-614.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48190N.
IntActi P0AD65. 2 interactions.
STRINGi 511145.b0635.

Chemistry

ChEMBLi CHEMBL1840.

Proteomic databases

PaxDbi P0AD65.
PRIDEi P0AD65.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73736 ; AAC73736 ; b0635 .
BAA35282 ; BAA35282 ; BAA35282 .
GeneIDi 12930918.
945240.
KEGGi ecj:Y75_p0625.
eco:b0635.
PATRICi 32116453. VBIEscCol129921_0666.

Organism-specific databases

EchoBASEi EB0601.
EcoGenei EG10606. mrdA.

Phylogenomic databases

eggNOGi COG0768.
HOGENOMi HOG000266120.
KOi K05515.
OMAi NDGQVKT.
OrthoDBi EOG6N0HHV.
PhylomeDBi P0AD65.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci EcoCyc:EG10606-MONOMER.
ECOL316407:JW0630-MONOMER.
MetaCyc:EG10606-MONOMER.

Miscellaneous databases

PROi P0AD65.

Gene expression databases

Genevestigatori P0AD65.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
[Graphical view ]
Pfami PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03423. pbp2_mrdA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12."
    Asoh S., Matsuzawa H., Ishino F., Strominger J.L., Matsuhashi M., Ohta T.
    Eur. J. Biochem. 160:231-238(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon."
    Matsuzawa H., Asoh S., Kunai K., Muraiso K., Takasuga A., Ohta T.
    J. Bacteriol. 171:558-560(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 606-633.
    Strain: K12.

Entry informationi

Entry nameiPBP2_ECOLI
AccessioniPrimary (citable) accession number: P0AD65
Secondary accession number(s): P08150
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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