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P0AD65

- PBP2_ECOLI

UniProt

P0AD65 - PBP2_ECOLI

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Protein

Penicillin-binding protein 2

Gene

mrdA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. It synthesizes cross-linked peptidoglycan from lipid intermediates.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei330 – 3301Acyl-ester intermediate1 Publication

GO - Molecular functioni

  1. drug binding Source: EcoCyc
  2. penicillin binding Source: EcoliWiki
  3. peptidoglycan L,D-transpeptidase activity Source: EcoliWiki
  4. serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc

GO - Biological processi

  1. cell wall organization Source: EcoCyc
  2. peptidoglycan biosynthetic process Source: EcoCyc
  3. proteolysis Source: GOC
  4. regulation of cell shape Source: EcoCyc
  5. response to antibiotic Source: EcoliWiki
  6. response to drug Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10606-MONOMER.
ECOL316407:JW0630-MONOMER.
MetaCyc:EG10606-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2
Short name:
PBP-2
Gene namesi
Name:mrdA
Synonyms:pbpA
Ordered Locus Names:b0635, JW0630
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10606. mrdA.

Subcellular locationi

Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi330 – 3301S → C: No longer binds penicillin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Penicillin-binding protein 2PRO_0000195444Add
BLAST

Proteomic databases

PaxDbiP0AD65.
PRIDEiP0AD65.

Expressioni

Gene expression databases

GenevestigatoriP0AD65.

Interactioni

Protein-protein interaction databases

DIPiDIP-48190N.
IntActiP0AD65. 2 interactions.
STRINGi511145.b0635.

Structurei

3D structure databases

ProteinModelPortaliP0AD65.
SMRiP0AD65. Positions 56-614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Sequence similaritiesi

Belongs to the transpeptidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0768.
HOGENOMiHOG000266120.
InParanoidiP0AD65.
KOiK05515.
OMAiNDGQVKT.
OrthoDBiEOG6N0HHV.
PhylomeDBiP0AD65.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
[Graphical view]
PfamiPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AD65-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY
60 70 80 90 100
QTRSNENRIK LVPIAPSRGI IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ
110 120 130 140 150
TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE VQVARFAVNQ
160 170 180 190 200
YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA
210 220 230 240 250
ATHDIGKLGI ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI
260 270 280 290 300
YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT GGVLALVSTP SYDPNLFVDG
310 320 330 340 350
ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT
360 370 380 390 400
TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG
410 420 430 440 450
IDRLSEWMGK FGYGHYTGID LAEERSGNMP TREWKQKRFK KPWYQGDTIP
460 470 480 490 500
VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED GKQVPWVQPH
510 520 530 540 550
EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV
560 570 580 590 600
FGLKANETYN AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV
610 620 630
GTLMRQILDH IMLGDNNTDL PAENPAVAAA EDH
Length:633
Mass (Da):70,857
Last modified:August 1, 1988 - v1
Checksum:iFE2305C002743AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04516 Genomic DNA. Translation: CAA28201.1.
U82598 Genomic DNA. Translation: AAB40835.1.
U00096 Genomic DNA. Translation: AAC73736.1.
AP009048 Genomic DNA. Translation: BAA35282.1.
M22857 Genomic DNA. Translation: AAA24570.1.
PIRiC24995. ZPECP2.
RefSeqiNP_415168.1. NC_000913.3.
YP_488926.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73736; AAC73736; b0635.
BAA35282; BAA35282; BAA35282.
GeneIDi12930918.
945240.
KEGGiecj:Y75_p0625.
eco:b0635.
PATRICi32116453. VBIEscCol129921_0666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04516 Genomic DNA. Translation: CAA28201.1 .
U82598 Genomic DNA. Translation: AAB40835.1 .
U00096 Genomic DNA. Translation: AAC73736.1 .
AP009048 Genomic DNA. Translation: BAA35282.1 .
M22857 Genomic DNA. Translation: AAA24570.1 .
PIRi C24995. ZPECP2.
RefSeqi NP_415168.1. NC_000913.3.
YP_488926.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AD65.
SMRi P0AD65. Positions 56-614.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48190N.
IntActi P0AD65. 2 interactions.
STRINGi 511145.b0635.

Chemistry

ChEMBLi CHEMBL2354204.
DrugBanki DB01327. Cefazolin.
DB01413. Cefepime.
DB01328. Cefonicid.
DB01329. Cefoperazone.
DB00438. Ceftazidime.
DB01415. Ceftibuten.
DB00303. Ertapenem.
DB01598. Imipenem.
DB00948. Mezlocillin.

Proteomic databases

PaxDbi P0AD65.
PRIDEi P0AD65.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73736 ; AAC73736 ; b0635 .
BAA35282 ; BAA35282 ; BAA35282 .
GeneIDi 12930918.
945240.
KEGGi ecj:Y75_p0625.
eco:b0635.
PATRICi 32116453. VBIEscCol129921_0666.

Organism-specific databases

EchoBASEi EB0601.
EcoGenei EG10606. mrdA.

Phylogenomic databases

eggNOGi COG0768.
HOGENOMi HOG000266120.
InParanoidi P0AD65.
KOi K05515.
OMAi NDGQVKT.
OrthoDBi EOG6N0HHV.
PhylomeDBi P0AD65.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci EcoCyc:EG10606-MONOMER.
ECOL316407:JW0630-MONOMER.
MetaCyc:EG10606-MONOMER.

Miscellaneous databases

PROi P0AD65.

Gene expression databases

Genevestigatori P0AD65.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
[Graphical view ]
Pfami PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03423. pbp2_mrdA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12."
    Asoh S., Matsuzawa H., Ishino F., Strominger J.L., Matsuhashi M., Ohta T.
    Eur. J. Biochem. 160:231-238(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Identification of the penicillin-binding active site of penicillin-binding protein 2 of Escherichia coli."
    Takasuga A., Adachi H., Ishino F., Matsuhashi M., Ohta T., Matsuzawa H.
    J. Biochem. 104:822-826(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 321-334 AND 633, ACTIVE SITE, PENICILLIN-BINDING, MUTAGENESIS OF SER-330.
  7. "Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon."
    Matsuzawa H., Asoh S., Kunai K., Muraiso K., Takasuga A., Ohta T.
    J. Bacteriol. 171:558-560(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 606-633.
    Strain: K12.
  8. "Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12."
    Spratt B.G.
    Proc. Natl. Acad. Sci. U.S.A. 72:2999-3003(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ANTIBIOTIC-BINDING.
    Strain: K12.

Entry informationi

Entry nameiPBP2_ECOLI
AccessioniPrimary (citable) accession number: P0AD65
Secondary accession number(s): P08150
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3