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P0AD65

- PBP2_ECOLI

UniProt

P0AD65 - PBP2_ECOLI

Protein

Penicillin-binding protein 2

Gene

mrdA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. It synthesizes cross-linked peptidoglycan from lipid intermediates.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei330 – 3301Acyl-ester intermediate1 Publication

    GO - Molecular functioni

    1. drug binding Source: EcoCyc
    2. penicillin binding Source: EcoliWiki
    3. peptidoglycan L,D-transpeptidase activity Source: EcoliWiki
    4. serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc

    GO - Biological processi

    1. cell wall organization Source: EcoCyc
    2. peptidoglycan biosynthetic process Source: EcoCyc
    3. proteolysis Source: GOC
    4. regulation of cell shape Source: EcoCyc
    5. response to antibiotic Source: EcoliWiki
    6. response to drug Source: EcoCyc

    Keywords - Biological processi

    Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10606-MONOMER.
    ECOL316407:JW0630-MONOMER.
    MetaCyc:EG10606-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 2
    Short name:
    PBP-2
    Gene namesi
    Name:mrdA
    Synonyms:pbpA
    Ordered Locus Names:b0635, JW0630
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10606. mrdA.

    Subcellular locationi

    Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of plasma membrane Source: EcoliWiki

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi330 – 3301S → C: No longer binds penicillin. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 633633Penicillin-binding protein 2PRO_0000195444Add
    BLAST

    Proteomic databases

    PaxDbiP0AD65.
    PRIDEiP0AD65.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AD65.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-48190N.
    IntActiP0AD65. 2 interactions.
    STRINGi511145.b0635.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AD65.
    SMRiP0AD65. Positions 56-614.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

    Sequence similaritiesi

    Belongs to the transpeptidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0768.
    HOGENOMiHOG000266120.
    KOiK05515.
    OMAiNDGQVKT.
    OrthoDBiEOG6N0HHV.
    PhylomeDBiP0AD65.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    IPR017790. Penicillin-binding_protein_2.
    [Graphical view]
    PfamiPF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AD65-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY    50
    QTRSNENRIK LVPIAPSRGI IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ 100
    TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE VQVARFAVNQ 150
    YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA 200
    ATHDIGKLGI ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI 250
    YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT GGVLALVSTP SYDPNLFVDG 300
    ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT 350
    TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG 400
    IDRLSEWMGK FGYGHYTGID LAEERSGNMP TREWKQKRFK KPWYQGDTIP 450
    VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED GKQVPWVQPH 500
    EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV 550
    FGLKANETYN AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV 600
    GTLMRQILDH IMLGDNNTDL PAENPAVAAA EDH 633
    Length:633
    Mass (Da):70,857
    Last modified:August 1, 1988 - v1
    Checksum:iFE2305C002743AF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04516 Genomic DNA. Translation: CAA28201.1.
    U82598 Genomic DNA. Translation: AAB40835.1.
    U00096 Genomic DNA. Translation: AAC73736.1.
    AP009048 Genomic DNA. Translation: BAA35282.1.
    M22857 Genomic DNA. Translation: AAA24570.1.
    PIRiC24995. ZPECP2.
    RefSeqiNP_415168.1. NC_000913.3.
    YP_488926.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73736; AAC73736; b0635.
    BAA35282; BAA35282; BAA35282.
    GeneIDi12930918.
    945240.
    KEGGiecj:Y75_p0625.
    eco:b0635.
    PATRICi32116453. VBIEscCol129921_0666.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04516 Genomic DNA. Translation: CAA28201.1 .
    U82598 Genomic DNA. Translation: AAB40835.1 .
    U00096 Genomic DNA. Translation: AAC73736.1 .
    AP009048 Genomic DNA. Translation: BAA35282.1 .
    M22857 Genomic DNA. Translation: AAA24570.1 .
    PIRi C24995. ZPECP2.
    RefSeqi NP_415168.1. NC_000913.3.
    YP_488926.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AD65.
    SMRi P0AD65. Positions 56-614.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48190N.
    IntActi P0AD65. 2 interactions.
    STRINGi 511145.b0635.

    Chemistry

    ChEMBLi CHEMBL1840.

    Proteomic databases

    PaxDbi P0AD65.
    PRIDEi P0AD65.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73736 ; AAC73736 ; b0635 .
    BAA35282 ; BAA35282 ; BAA35282 .
    GeneIDi 12930918.
    945240.
    KEGGi ecj:Y75_p0625.
    eco:b0635.
    PATRICi 32116453. VBIEscCol129921_0666.

    Organism-specific databases

    EchoBASEi EB0601.
    EcoGenei EG10606. mrdA.

    Phylogenomic databases

    eggNOGi COG0768.
    HOGENOMi HOG000266120.
    KOi K05515.
    OMAi NDGQVKT.
    OrthoDBi EOG6N0HHV.
    PhylomeDBi P0AD65.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci EcoCyc:EG10606-MONOMER.
    ECOL316407:JW0630-MONOMER.
    MetaCyc:EG10606-MONOMER.

    Miscellaneous databases

    PROi P0AD65.

    Gene expression databases

    Genevestigatori P0AD65.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    IPR017790. Penicillin-binding_protein_2.
    [Graphical view ]
    Pfami PF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03423. pbp2_mrdA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12."
      Asoh S., Matsuzawa H., Ishino F., Strominger J.L., Matsuhashi M., Ohta T.
      Eur. J. Biochem. 160:231-238(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Identification of the penicillin-binding active site of penicillin-binding protein 2 of Escherichia coli."
      Takasuga A., Adachi H., Ishino F., Matsuhashi M., Ohta T., Matsuzawa H.
      J. Biochem. 104:822-826(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 321-334 AND 633, ACTIVE SITE, PENICILLIN-BINDING, MUTAGENESIS OF SER-330.
    7. "Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon."
      Matsuzawa H., Asoh S., Kunai K., Muraiso K., Takasuga A., Ohta T.
      J. Bacteriol. 171:558-560(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 606-633.
      Strain: K12.
    8. "Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12."
      Spratt B.G.
      Proc. Natl. Acad. Sci. U.S.A. 72:2999-3003(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, ANTIBIOTIC-BINDING.
      Strain: K12.

    Entry informationi

    Entry nameiPBP2_ECOLI
    AccessioniPrimary (citable) accession number: P0AD65
    Secondary accession number(s): P08150
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3