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Protein

Peptidoglycan D,D-transpeptidase MrdA

Gene

mrdA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes cross-linking of the peptidoglycan cell wall (PubMed:3009484). Responsible for the determination of the rod shape of the cell (PubMed:1103132). Is probably required for lateral peptidoglycan synthesis and maintenance of the correct diameter during lateral and centripetal growth (PubMed:12519203).3 Publications

Catalytic activityi

Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation1 Publication

Enzyme regulationi

Inhibited by mecillinam and benzylpenicillin.2 Publications

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei330Acyl-ester intermediateUniRule annotation1 Publication1

GO - Molecular functioni

  • drug binding Source: EcoCyc
  • penicillin binding Source: EcoliWiki
  • peptidoglycan L,D-transpeptidase activity Source: EcoliWiki
  • serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: EcoCyc
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: EcoliWiki
  • response to antibiotic Source: EcoliWiki
  • response to drug Source: EcoCyc

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10606-MONOMER
MetaCyc:EG10606-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase MrdAUniRule annotationCurated (EC:3.4.16.4UniRule annotation1 Publication)
Alternative name(s):
Penicillin-binding protein 22 PublicationsUniRule annotation
Short name:
PBP-21 PublicationUniRule annotation
Gene namesi
Name:mrdA1 PublicationUniRule annotation
Synonyms:pbpA2 Publications
Ordered Locus Names:b0635, JW0630
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10606 mrdA

Subcellular locationi

  • Cell inner membrane UniRule annotation2 Publications; Single-pass membrane protein UniRule annotation
  • Note: Localizes preferentially in the lateral wall and at mid-cell in comparison with the old cell pole. Localization at mid-cell is dependent on active FtsI.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei22 – 42HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Temperature-sensitive mutants grow as normal rods at 30 degrees Celsius but grow and divide as cocci during prolonged culturing at 42 degrees Celsius.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi330S → C: No longer binds penicillin. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1840
DrugBankiDB01163 Amdinocillin
DB01327 Cefazolin
DB01413 Cefepime
DB01328 Cefonicid
DB01329 Cefoperazone
DB00438 Ceftazidime
DB01415 Ceftibuten
DB06211 Doripenem
DB00303 Ertapenem
DB01598 Imipenem
DB00948 Mezlocillin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954441 – 633Peptidoglycan D,D-transpeptidase MrdAAdd BLAST633

Proteomic databases

PaxDbiP0AD65
PRIDEiP0AD65

Interactioni

Protein-protein interaction databases

BioGridi4259912573 interactors.
DIPiDIP-48190N
IntActiP0AD65 2 interactors.
STRINGi316385.ECDH10B_0704

Structurei

3D structure databases

ProteinModelPortaliP0AD65
SMRiP0AD65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transpeptidase family. MrdA subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN Bacteria
COG0768 LUCA
HOGENOMiHOG000266120
InParanoidiP0AD65
KOiK05515
OMAiSCDTYYY
PhylomeDBiP0AD65

Family and domain databases

HAMAPiMF_02081 MrdA_transpept, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR005311 PBP_dimer
IPR036138 PBP_dimer_sf
IPR001460 PCN-bd_Tpept
IPR017790 Penicillin-binding_protein_2
PfamiView protein in Pfam
PF03717 PBP_dimer, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF56519 SSF56519, 1 hit
SSF56601 SSF56601, 1 hit
TIGRFAMsiTIGR03423 pbp2_mrdA, 1 hit

Sequencei

Sequence statusi: Complete.

P0AD65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY
60 70 80 90 100
QTRSNENRIK LVPIAPSRGI IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ
110 120 130 140 150
TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE VQVARFAVNQ
160 170 180 190 200
YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA
210 220 230 240 250
ATHDIGKLGI ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI
260 270 280 290 300
YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT GGVLALVSTP SYDPNLFVDG
310 320 330 340 350
ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT
360 370 380 390 400
TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG
410 420 430 440 450
IDRLSEWMGK FGYGHYTGID LAEERSGNMP TREWKQKRFK KPWYQGDTIP
460 470 480 490 500
VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED GKQVPWVQPH
510 520 530 540 550
EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV
560 570 580 590 600
FGLKANETYN AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV
610 620 630
GTLMRQILDH IMLGDNNTDL PAENPAVAAA EDH
Length:633
Mass (Da):70,857
Last modified:August 1, 1988 - v1
Checksum:iFE2305C002743AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04516 Genomic DNA Translation: CAA28201.1
U82598 Genomic DNA Translation: AAB40835.1
U00096 Genomic DNA Translation: AAC73736.1
AP009048 Genomic DNA Translation: BAA35282.1
M22857 Genomic DNA Translation: AAA24570.1
PIRiC24995 ZPECP2
RefSeqiNP_415168.1, NC_000913.3
WP_000776191.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73736; AAC73736; b0635
BAA35282; BAA35282; BAA35282
GeneIDi945240
KEGGiecj:JW0630
eco:b0635
PATRICifig|1411691.4.peg.1633

Similar proteinsi

Entry informationi

Entry nameiMRDA_ECOLI
AccessioniPrimary (citable) accession number: P0AD65
Secondary accession number(s): P08150
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome