P0AD64 (BLA1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase SHV-1
EC=3.5.2.6

Alternative name(s):
PIT-2

Gene names

Name:	bla
Synonyms:	shv1

Encoded on

Plasmid R974 Ref.1

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Chain

22 – 286

265

Beta-lactamase SHV-1

PRO_0000043362

Regions

Region

230 – 232

3

Substrate binding

Sites

Active site

66

1

Acyl-ester intermediate

Active site

164

1

Proton acceptor

Amino acid modifications

Disulfide bond

73 ↔ 119

Experimental info

Sequence conflict

112

1

G → A in AAA26087. Ref.1

Sequence conflict

188 – 189

2

KL → NVG in AAA26087. Ref.1

Sequence conflict

278

1

A → K in AAA26087. Ref.1

Sequence conflict

281

1

I → Y in AAA26087. Ref.1

Secondary structure

1

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286

Helix Strand Turn

Details...

Helix

25 – 36

12

Beta strand

38 – 46

9

Turn

47 – 49

3

Beta strand

52 – 57

6

Helix

65 – 67

3

Helix

68 – 81

14

Helix

95 – 97

3

Helix

105 – 108

4

Turn

109 – 111

3

Helix

115 – 125

11

Helix

128 – 137

10

Helix

140 – 150

11

Helix

164 – 166

3

Helix

179 – 191

13

Beta strand

192 – 195

4

Helix

197 – 208

12

Beta strand

211 – 213

3

Helix

214 – 220

7

Beta strand

226 – 233

8

Helix

235 – 237

3

Beta strand

239 – 247

9

Beta strand

253 – 261

9

Helix

266 – 282

17

Turn

283 – 285

3

Sequences

	Sequence		Length	Mass (Da)
	P0AD64 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C78F42667E698E6C	FASTA	286	31,224
	10 20 30 40 50 60 MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR

References

[1]	"Cloning of SHV-2, OHIO-1, and OXA-6 beta-lactamases and cloning and sequencing of SHV-1 beta-lactamase." Mercier J., Levesque R.C. Antimicrob. Agents Chemother. 34:1577-1583(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12." Nuesch-Inderbinen M., Kayser F.H., Hachler H. Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KPAA-1, KPZU-13 and KPZU-8.
[3]	"High-level expression of chromosomally encoded SHV-1 beta-lactamase and an outer membrane protein change confer resistance to ceftazidime and piperacillin-tazobactam in a clinical isolate of Klebsiella pneumoniae." Rice L.B., Carias L.L., Hujer A.M., Bonafede M., Hutton R., Hoyen C., Bonomo R.A. Antimicrob. Agents Chemother. 44:362-367(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 15571.
[4]	"Structure of the SHV-1 beta-lactamase." Kuzin A.P., Nukaga M., Nukaga Y., Hujer A.M., Bonomo R.A., Knox J.R. Biochemistry 38:5720-5727(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS). Strain: 15571.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M59181 Genomic DNA. Translation: AAA26087.1.
X98098 Genomic DNA. Translation: CAA66726.1.
X98099 Genomic DNA. Translation: CAA66727.1.
X98100 Genomic DNA. Translation: CAA66728.1.
AF124984 Genomic DNA. Translation: AAD18054.1.

PIR

A44996.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ONG	X-ray	1.10	A	22-286	[»]
1Q2P	X-ray	2.00	A	22-286	[»]
1RCJ	X-ray	1.63	A	22-286	[»]
1SHV	X-ray	1.98	A	22-286	[»]
1TDG	X-ray	1.80	A	22-286	[»]
1TDL	X-ray	1.80	A	22-286	[»]
1VM1	X-ray	2.02	A	22-286	[»]
2A3U	X-ray	1.34	A	22-286	[»]
2A49	X-ray	1.43	A	22-286	[»]
2G2U	X-ray	1.60	A	22-284	[»]
2G2W	X-ray	1.80	A	22-284	[»]
2H0T	X-ray	1.60	A	22-286	[»]
2H0Y	X-ray	1.70	A	22-284	[»]
2H10	X-ray	1.75	A	22-284	[»]
2H5S	X-ray	1.28	A	22-286	[»]
2ZD8	X-ray	1.05	A	22-286	[»]
3C4O	X-ray	1.70	A	22-286	[»]
3C4P	X-ray	1.75	A	22-286	[»]
3D4F	X-ray	1.55	A	1-286	[»]
3MKE	X-ray	1.75	A	22-286	[»]
3MKF	X-ray	1.33	A	22-286	[»]
3MXR	X-ray	1.30	A	22-286	[»]
3MXS	X-ray	1.24	A	22-286	[»]
3N4I	X-ray	1.56	A	22-286	[»]
3OPH	X-ray	1.34	A	1-286	[»]
3OPL	X-ray	1.80	A	1-286	[»]
3OPP	X-ray	1.80	A	1-286	[»]
3OPR	X-ray	1.65	A	1-286	[»]
3V50	X-ray	1.45	A	22-286	[»]
3V5M	X-ray	1.30	A	22-286	[»]
4FCF	X-ray	1.09	A	22-286	[»]
4FD8	X-ray	1.52	A	22-286	[»]
4FH2	X-ray	1.44	A	22-286	[»]
4FH4	X-ray	1.09	A	22-286	[»]

ProteinModelPortal

P0AD64.

SMR

P0AD64. Positions 22-286.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

SABIO-RK

P0AD64.

Family and domain databases

Gene3D

3.40.710.10. 1 hit.

InterPro

IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P0AD64.

ChEMBL

CHEMBL5094.

EvolutionaryTrace

P0AD64.

Entry information

Entry name

BLA1_KLEPN

Accession

Primary (citable) accession number: P0AD64
Secondary accession number(s): O07941, P14557, P23982

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program