Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-lactamase SHV-1

Gene

bla

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Acyl-ester intermediate1
Active sitei164Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6. 2814.
SABIO-RKP0AD64.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase SHV-1 (EC:3.5.2.6)
Alternative name(s):
PIT-2
Gene namesi
Name:bla
Synonyms:shv1
Encoded oniPlasmid R9741 Publication
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5094.
DrugBankiDB09060. Avibactam.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000004336222 – 286Beta-lactamase SHV-1Add BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 119

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi272620.KPN_01607.

Chemistry databases

BindingDBiP0AD64.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 36Combined sources12
Beta strandi38 – 46Combined sources9
Turni47 – 49Combined sources3
Beta strandi52 – 57Combined sources6
Helixi65 – 67Combined sources3
Helixi68 – 81Combined sources14
Helixi95 – 97Combined sources3
Helixi105 – 108Combined sources4
Turni109 – 111Combined sources3
Helixi115 – 125Combined sources11
Helixi128 – 137Combined sources10
Helixi140 – 150Combined sources11
Helixi164 – 166Combined sources3
Helixi179 – 191Combined sources13
Beta strandi192 – 195Combined sources4
Helixi197 – 208Combined sources12
Beta strandi211 – 213Combined sources3
Helixi214 – 220Combined sources7
Beta strandi226 – 233Combined sources8
Helixi235 – 237Combined sources3
Beta strandi239 – 247Combined sources9
Beta strandi253 – 261Combined sources9
Helixi266 – 282Combined sources17
Turni283 – 285Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ONGX-ray1.10A22-286[»]
1Q2PX-ray2.00A22-286[»]
1RCJX-ray1.63A22-286[»]
1SHVX-ray1.98A22-286[»]
1TDGX-ray1.80A22-286[»]
1TDLX-ray1.80A22-286[»]
1VM1X-ray2.02A22-286[»]
2A3UX-ray1.34A22-286[»]
2A49X-ray1.43A22-286[»]
2G2UX-ray1.60A22-284[»]
2G2WX-ray1.80A22-284[»]
2H0TX-ray1.60A22-286[»]
2H0YX-ray1.70A22-284[»]
2H10X-ray1.75A22-284[»]
2H5SX-ray1.28A22-286[»]
2ZD8X-ray1.05A22-286[»]
3C4OX-ray1.70A22-286[»]
3C4PX-ray1.75A22-286[»]
3D4FX-ray1.55A1-286[»]
3MKEX-ray1.75A22-286[»]
3MKFX-ray1.33A22-286[»]
3MXRX-ray1.30A22-286[»]
3MXSX-ray1.24A22-286[»]
3N4IX-ray1.56A22-286[»]
3OPHX-ray1.34A1-286[»]
3OPLX-ray1.80A1-286[»]
3OPPX-ray1.80A1-286[»]
3OPRX-ray1.65A1-286[»]
3V50X-ray1.45A22-286[»]
3V5MX-ray1.30A22-286[»]
4FCFX-ray1.09A22-286[»]
4FD8X-ray1.52A22-286[»]
4FH2X-ray1.44A22-286[»]
4FH4X-ray1.09A22-286[»]
4GD6X-ray1.53A1-286[»]
4GD8X-ray1.60A1-286[»]
4GDBX-ray1.84A1-286[»]
4JPMX-ray1.14A1-286[»]
4MBFX-ray1.54A22-286[»]
4MBHX-ray1.22A22-286[»]
4MBKX-ray1.46A22-286[»]
4R3BX-ray1.37A22-286[»]
4ZAMX-ray1.42A22-286[»]
5EE8X-ray1.54A22-286[»]
ProteinModelPortaliP0AD64.
SMRiP0AD64.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AD64.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 232Substrate binding3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG
60 70 80 90 100
RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD
110 120 130 140 150
YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ
160 170 180 190 200
IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ
210 220 230 240 250
RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK
260 270 280
AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
Length:286
Mass (Da):31,224
Last modified:December 6, 2005 - v1
Checksum:iC78F42667E698E6C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112G → A in AAA26087 (PubMed:2221867).Curated1
Sequence conflicti188 – 189KL → NVG in AAA26087 (PubMed:2221867).Curated2
Sequence conflicti278A → K in AAA26087 (PubMed:2221867).Curated1
Sequence conflicti281I → Y in AAA26087 (PubMed:2221867).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59181 Genomic DNA. Translation: AAA26087.1.
X98098 Genomic DNA. Translation: CAA66726.1.
X98099 Genomic DNA. Translation: CAA66727.1.
X98100 Genomic DNA. Translation: CAA66728.1.
AF124984 Genomic DNA. Translation: AAD18054.1.
PIRiA44996.
RefSeqiWP_001620095.1. NZ_LZCT01000013.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59181 Genomic DNA. Translation: AAA26087.1.
X98098 Genomic DNA. Translation: CAA66726.1.
X98099 Genomic DNA. Translation: CAA66727.1.
X98100 Genomic DNA. Translation: CAA66728.1.
AF124984 Genomic DNA. Translation: AAD18054.1.
PIRiA44996.
RefSeqiWP_001620095.1. NZ_LZCT01000013.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ONGX-ray1.10A22-286[»]
1Q2PX-ray2.00A22-286[»]
1RCJX-ray1.63A22-286[»]
1SHVX-ray1.98A22-286[»]
1TDGX-ray1.80A22-286[»]
1TDLX-ray1.80A22-286[»]
1VM1X-ray2.02A22-286[»]
2A3UX-ray1.34A22-286[»]
2A49X-ray1.43A22-286[»]
2G2UX-ray1.60A22-284[»]
2G2WX-ray1.80A22-284[»]
2H0TX-ray1.60A22-286[»]
2H0YX-ray1.70A22-284[»]
2H10X-ray1.75A22-284[»]
2H5SX-ray1.28A22-286[»]
2ZD8X-ray1.05A22-286[»]
3C4OX-ray1.70A22-286[»]
3C4PX-ray1.75A22-286[»]
3D4FX-ray1.55A1-286[»]
3MKEX-ray1.75A22-286[»]
3MKFX-ray1.33A22-286[»]
3MXRX-ray1.30A22-286[»]
3MXSX-ray1.24A22-286[»]
3N4IX-ray1.56A22-286[»]
3OPHX-ray1.34A1-286[»]
3OPLX-ray1.80A1-286[»]
3OPPX-ray1.80A1-286[»]
3OPRX-ray1.65A1-286[»]
3V50X-ray1.45A22-286[»]
3V5MX-ray1.30A22-286[»]
4FCFX-ray1.09A22-286[»]
4FD8X-ray1.52A22-286[»]
4FH2X-ray1.44A22-286[»]
4FH4X-ray1.09A22-286[»]
4GD6X-ray1.53A1-286[»]
4GD8X-ray1.60A1-286[»]
4GDBX-ray1.84A1-286[»]
4JPMX-ray1.14A1-286[»]
4MBFX-ray1.54A22-286[»]
4MBHX-ray1.22A22-286[»]
4MBKX-ray1.46A22-286[»]
4R3BX-ray1.37A22-286[»]
4ZAMX-ray1.42A22-286[»]
5EE8X-ray1.54A22-286[»]
ProteinModelPortaliP0AD64.
SMRiP0AD64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_01607.

Chemistry databases

BindingDBiP0AD64.
ChEMBLiCHEMBL5094.
DrugBankiDB09060. Avibactam.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.

Enzyme and pathway databases

BRENDAi3.5.2.6. 2814.
SABIO-RKP0AD64.

Miscellaneous databases

EvolutionaryTraceiP0AD64.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLA1_KLEPN
AccessioniPrimary (citable) accession number: P0AD64
Secondary accession number(s): O07941, P14557, P23982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.