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P0AD64 (BLA1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase SHV-1

EC=3.5.2.6
Alternative name(s):
PIT-2
Gene names
Name:bla
Synonyms:shv1
Encoded onPlasmid R974 Ref.1
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 286265Beta-lactamase SHV-1
PRO_0000043362

Regions

Region230 – 2323Substrate binding

Sites

Active site661Acyl-ester intermediate
Active site1641Proton acceptor

Amino acid modifications

Disulfide bond73 ↔ 119

Experimental info

Sequence conflict1121G → A in AAA26087. Ref.1
Sequence conflict188 – 1892KL → NVG in AAA26087. Ref.1
Sequence conflict2781A → K in AAA26087. Ref.1
Sequence conflict2811I → Y in AAA26087. Ref.1

Secondary structure

........................................... 286
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AD64 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: C78F42667E698E6C

FASTA28631,224
        10         20         30         40         50         60 
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 

        70         80         90        100        110        120 
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 

       130        140        150        160        170        180 
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 

       190        200        210        220        230        240 
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG 

       250        260        270        280 
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 

« Hide

References

[1]"Cloning of SHV-2, OHIO-1, and OXA-6 beta-lactamases and cloning and sequencing of SHV-1 beta-lactamase."
Mercier J., Levesque R.C.
Antimicrob. Agents Chemother. 34:1577-1583(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12."
Nuesch-Inderbinen M., Kayser F.H., Hachler H.
Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KPAA-1, KPZU-13 and KPZU-8.
[3]"High-level expression of chromosomally encoded SHV-1 beta-lactamase and an outer membrane protein change confer resistance to ceftazidime and piperacillin-tazobactam in a clinical isolate of Klebsiella pneumoniae."
Rice L.B., Carias L.L., Hujer A.M., Bonafede M., Hutton R., Hoyen C., Bonomo R.A.
Antimicrob. Agents Chemother. 44:362-367(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 15571.
[4]"Structure of the SHV-1 beta-lactamase."
Kuzin A.P., Nukaga M., Nukaga Y., Hujer A.M., Bonomo R.A., Knox J.R.
Biochemistry 38:5720-5727(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS).
Strain: 15571.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59181 Genomic DNA. Translation: AAA26087.1.
X98098 Genomic DNA. Translation: CAA66726.1.
X98099 Genomic DNA. Translation: CAA66727.1.
X98100 Genomic DNA. Translation: CAA66728.1.
AF124984 Genomic DNA. Translation: AAD18054.1.
PIRA44996.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ONGX-ray1.10A22-286[»]
1Q2PX-ray2.00A22-286[»]
1RCJX-ray1.63A22-286[»]
1SHVX-ray1.98A22-286[»]
1TDGX-ray1.80A22-286[»]
1TDLX-ray1.80A22-286[»]
1VM1X-ray2.02A22-286[»]
2A3UX-ray1.34A22-286[»]
2A49X-ray1.43A22-286[»]
2G2UX-ray1.60A22-284[»]
2G2WX-ray1.80A22-284[»]
2H0TX-ray1.60A22-286[»]
2H0YX-ray1.70A22-284[»]
2H10X-ray1.75A22-284[»]
2H5SX-ray1.28A22-286[»]
2ZD8X-ray1.05A22-286[»]
3C4OX-ray1.70A22-286[»]
3C4PX-ray1.75A22-286[»]
3D4FX-ray1.55A1-286[»]
3MKEX-ray1.75A22-286[»]
3MKFX-ray1.33A22-286[»]
3MXRX-ray1.30A22-286[»]
3MXSX-ray1.24A22-286[»]
3N4IX-ray1.56A22-286[»]
3OPHX-ray1.34A1-286[»]
3OPLX-ray1.80A1-286[»]
3OPPX-ray1.80A1-286[»]
3OPRX-ray1.65A1-286[»]
3V50X-ray1.45A22-286[»]
3V5MX-ray1.30A22-286[»]
4FCFX-ray1.09A22-286[»]
4FD8X-ray1.52A22-286[»]
4FH2X-ray1.44A22-286[»]
4FH4X-ray1.09A22-286[»]
4GD6X-ray1.53A1-286[»]
4GD8X-ray1.60A1-286[»]
4GDBX-ray1.84A1-286[»]
4JPMX-ray1.14A1-286[»]
ProteinModelPortalP0AD64.
SMRP0AD64. Positions 22-286.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP0AD64.
ChEMBLCHEMBL5094.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP0AD64.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0AD64.

Entry information

Entry nameBLA1_KLEPN
AccessionPrimary (citable) accession number: P0AD64
Secondary accession number(s): O07941, P14557, P23982
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references