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P0AD64

- BLA1_KLEPN

UniProt

P0AD64 - BLA1_KLEPN

Protein

Beta-lactamase SHV-1

Gene

bla

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Acyl-ester intermediate
    Active sitei164 – 1641Proton acceptor

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    SABIO-RKP0AD64.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase SHV-1 (EC:3.5.2.6)
    Alternative name(s):
    PIT-2
    Gene namesi
    Name:bla
    Synonyms:shv1
    Encoded oniPlasmid R9741 Publication
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 286265Beta-lactamase SHV-1PRO_0000043362Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 119

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Structurei

    Secondary structure

    1
    286
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi25 – 3612
    Beta strandi38 – 469
    Turni47 – 493
    Beta strandi52 – 576
    Helixi65 – 673
    Helixi68 – 8114
    Helixi95 – 973
    Helixi105 – 1084
    Turni109 – 1113
    Helixi115 – 12511
    Helixi128 – 13710
    Helixi140 – 15011
    Helixi164 – 1663
    Helixi179 – 19113
    Beta strandi192 – 1954
    Helixi197 – 20812
    Beta strandi211 – 2133
    Helixi214 – 2207
    Beta strandi226 – 2338
    Helixi235 – 2373
    Beta strandi239 – 2479
    Beta strandi253 – 2619
    Helixi266 – 28217
    Turni283 – 2853

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ONGX-ray1.10A22-286[»]
    1Q2PX-ray2.00A22-286[»]
    1RCJX-ray1.63A22-286[»]
    1SHVX-ray1.98A22-286[»]
    1TDGX-ray1.80A22-286[»]
    1TDLX-ray1.80A22-286[»]
    1VM1X-ray2.02A22-286[»]
    2A3UX-ray1.34A22-286[»]
    2A49X-ray1.43A22-286[»]
    2G2UX-ray1.60A22-284[»]
    2G2WX-ray1.80A22-284[»]
    2H0TX-ray1.60A22-286[»]
    2H0YX-ray1.70A22-284[»]
    2H10X-ray1.75A22-284[»]
    2H5SX-ray1.28A22-286[»]
    2ZD8X-ray1.05A22-286[»]
    3C4OX-ray1.70A22-286[»]
    3C4PX-ray1.75A22-286[»]
    3D4FX-ray1.55A1-286[»]
    3MKEX-ray1.75A22-286[»]
    3MKFX-ray1.33A22-286[»]
    3MXRX-ray1.30A22-286[»]
    3MXSX-ray1.24A22-286[»]
    3N4IX-ray1.56A22-286[»]
    3OPHX-ray1.34A1-286[»]
    3OPLX-ray1.80A1-286[»]
    3OPPX-ray1.80A1-286[»]
    3OPRX-ray1.65A1-286[»]
    3V50X-ray1.45A22-286[»]
    3V5MX-ray1.30A22-286[»]
    4FCFX-ray1.09A22-286[»]
    4FD8X-ray1.52A22-286[»]
    4FH2X-ray1.44A22-286[»]
    4FH4X-ray1.09A22-286[»]
    4GD6X-ray1.53A1-286[»]
    4GD8X-ray1.60A1-286[»]
    4GDBX-ray1.84A1-286[»]
    4JPMX-ray1.14A1-286[»]
    ProteinModelPortaliP0AD64.
    SMRiP0AD64. Positions 22-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AD64.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni230 – 2323Substrate binding

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AD64-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG    50
    RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD 100
    YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ 150
    IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ 200
    RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK 250
    AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 286
    Length:286
    Mass (Da):31,224
    Last modified:December 6, 2005 - v1
    Checksum:iC78F42667E698E6C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121G → A in AAA26087. (PubMed:2221867)Curated
    Sequence conflicti188 – 1892KL → NVG in AAA26087. (PubMed:2221867)Curated
    Sequence conflicti278 – 2781A → K in AAA26087. (PubMed:2221867)Curated
    Sequence conflicti281 – 2811I → Y in AAA26087. (PubMed:2221867)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59181 Genomic DNA. Translation: AAA26087.1.
    X98098 Genomic DNA. Translation: CAA66726.1.
    X98099 Genomic DNA. Translation: CAA66727.1.
    X98100 Genomic DNA. Translation: CAA66728.1.
    AF124984 Genomic DNA. Translation: AAD18054.1.
    PIRiA44996.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59181 Genomic DNA. Translation: AAA26087.1 .
    X98098 Genomic DNA. Translation: CAA66726.1 .
    X98099 Genomic DNA. Translation: CAA66727.1 .
    X98100 Genomic DNA. Translation: CAA66728.1 .
    AF124984 Genomic DNA. Translation: AAD18054.1 .
    PIRi A44996.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ONG X-ray 1.10 A 22-286 [» ]
    1Q2P X-ray 2.00 A 22-286 [» ]
    1RCJ X-ray 1.63 A 22-286 [» ]
    1SHV X-ray 1.98 A 22-286 [» ]
    1TDG X-ray 1.80 A 22-286 [» ]
    1TDL X-ray 1.80 A 22-286 [» ]
    1VM1 X-ray 2.02 A 22-286 [» ]
    2A3U X-ray 1.34 A 22-286 [» ]
    2A49 X-ray 1.43 A 22-286 [» ]
    2G2U X-ray 1.60 A 22-284 [» ]
    2G2W X-ray 1.80 A 22-284 [» ]
    2H0T X-ray 1.60 A 22-286 [» ]
    2H0Y X-ray 1.70 A 22-284 [» ]
    2H10 X-ray 1.75 A 22-284 [» ]
    2H5S X-ray 1.28 A 22-286 [» ]
    2ZD8 X-ray 1.05 A 22-286 [» ]
    3C4O X-ray 1.70 A 22-286 [» ]
    3C4P X-ray 1.75 A 22-286 [» ]
    3D4F X-ray 1.55 A 1-286 [» ]
    3MKE X-ray 1.75 A 22-286 [» ]
    3MKF X-ray 1.33 A 22-286 [» ]
    3MXR X-ray 1.30 A 22-286 [» ]
    3MXS X-ray 1.24 A 22-286 [» ]
    3N4I X-ray 1.56 A 22-286 [» ]
    3OPH X-ray 1.34 A 1-286 [» ]
    3OPL X-ray 1.80 A 1-286 [» ]
    3OPP X-ray 1.80 A 1-286 [» ]
    3OPR X-ray 1.65 A 1-286 [» ]
    3V50 X-ray 1.45 A 22-286 [» ]
    3V5M X-ray 1.30 A 22-286 [» ]
    4FCF X-ray 1.09 A 22-286 [» ]
    4FD8 X-ray 1.52 A 22-286 [» ]
    4FH2 X-ray 1.44 A 22-286 [» ]
    4FH4 X-ray 1.09 A 22-286 [» ]
    4GD6 X-ray 1.53 A 1-286 [» ]
    4GD8 X-ray 1.60 A 1-286 [» ]
    4GDB X-ray 1.84 A 1-286 [» ]
    4JPM X-ray 1.14 A 1-286 [» ]
    ProteinModelPortali P0AD64.
    SMRi P0AD64. Positions 22-286.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P0AD64.
    ChEMBLi CHEMBL5094.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P0AD64.

    Miscellaneous databases

    EvolutionaryTracei P0AD64.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of SHV-2, OHIO-1, and OXA-6 beta-lactamases and cloning and sequencing of SHV-1 beta-lactamase."
      Mercier J., Levesque R.C.
      Antimicrob. Agents Chemother. 34:1577-1583(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: R974
    2. "Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12."
      Nuesch-Inderbinen M., Kayser F.H., Hachler H.
      Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KPAA-1, KPZU-13 and KPZU-8.
    3. "High-level expression of chromosomally encoded SHV-1 beta-lactamase and an outer membrane protein change confer resistance to ceftazidime and piperacillin-tazobactam in a clinical isolate of Klebsiella pneumoniae."
      Rice L.B., Carias L.L., Hujer A.M., Bonafede M., Hutton R., Hoyen C., Bonomo R.A.
      Antimicrob. Agents Chemother. 44:362-367(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 15571.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS).
      Strain: 15571.

    Entry informationi

    Entry nameiBLA1_KLEPN
    AccessioniPrimary (citable) accession number: P0AD64
    Secondary accession number(s): O07941, P14557, P23982
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3