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P0AD63

- BLA1_ECOLX

UniProt

P0AD63 - BLA1_ECOLX

Protein

Beta-lactamase SHV-1

Gene

bla

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei164 – 1641Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase SHV-1 (EC:3.5.2.6)
    Alternative name(s):
    PIT-2
    Gene namesi
    Name:bla
    Synonyms:shv1
    Encoded oniPlasmid p4532 Publications
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21212 PublicationsAdd
    BLAST
    Chaini22 – 286265Beta-lactamase SHV-1PRO_0000016980Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 119By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP0AD63.
    SMRiP0AD63. Positions 22-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni230 – 2323Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AD63-1 [UniParc]FASTAAdd to Basket

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    MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG    50
    RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD 100
    YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ 150
    IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ 200
    RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK 250
    AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 286
    Length:286
    Mass (Da):31,224
    Last modified:December 6, 2005 - v1
    Checksum:iC78F42667E698E6C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti136 – 1372AT → TA AA sequence (PubMed:3260490)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148850 Genomic DNA. Translation: AAD37412.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148850 Genomic DNA. Translation: AAD37412.1 .

    3D structure databases

    ProteinModelPortali P0AD63.
    SMRi P0AD63. Positions 22-286.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P0AD63.
    ChEMBLi CHEMBL5956.
    DrugBanki DB01606. Tazobactam.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Automated thermal cycling is superior to traditional methods for nucleotide sequencing of bla(SHV) genes."
      Bradford P.A.
      Antimicrob. Agents Chemother. 43:2960-2963(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33694 / HB101.
    2. "Complete amino acid sequence of p453-plasmid-mediated PIT-2 beta-lactamase (SHV-1)."
      Barthelemy M., Peduzzi J., Labia R.
      Biochem. J. 251:73-79(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-286.
      Plasmid: p453
    3. "N-terminal amino acid sequence of PIT-2 beta-lactamase (SHV-1)."
      Barthelemy M., Peduzzi J., Labia R.
      J. Antimicrob. Chemother. 19:839-841(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-128.
      Plasmid: p453

    Entry informationi

    Entry nameiBLA1_ECOLX
    AccessioniPrimary (citable) accession number: P0AD63
    Secondary accession number(s): O07941, P14557, P23982
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3