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Protein

Inhibitor of vertebrate lysozyme

Gene

ivy

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Strong inhibitor of lysozyme C.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei88 – 881Important for lysozyme inhibition

GO - Molecular functioni

  1. lysozyme inhibitor activity Source: EcoCyc

GO - Biological processi

  1. regulation of catalytic activity Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6104-MONOMER.
ECOL316407:JW0210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of vertebrate lysozyme
Gene namesi
Name:ivy
Synonyms:ykfE
Ordered Locus Names:b0220, JW0210
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13547. ivy.

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28282 PublicationsAdd
BLAST
Chaini29 – 157129Inhibitor of vertebrate lysozymePRO_0000016546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi85 ↔ 90

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0AD59.
PRIDEiP0AD59.

2D gel databases

SWISS-2DPAGEP0AD59.

Expressioni

Gene expression databases

GenevestigatoriP0AD59.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-10051N.
STRINGi511145.b0220.

Structurei

Secondary structure

1
157
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 396Combined sources
Turni41 – 433Combined sources
Helixi44 – 518Combined sources
Helixi59 – 624Combined sources
Beta strandi65 – 7511Combined sources
Beta strandi77 – 859Combined sources
Turni90 – 923Combined sources
Beta strandi93 – 997Combined sources
Turni101 – 1033Combined sources
Beta strandi106 – 1138Combined sources
Turni115 – 1173Combined sources
Beta strandi120 – 1256Combined sources
Helixi129 – 1313Combined sources
Helixi133 – 14412Combined sources
Helixi146 – 1494Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GPQX-ray1.60A/B29-156[»]
1XS0X-ray1.58A/B/C29-156[»]
ProteinModelPortaliP0AD59.
SMRiP0AD59. Positions 30-156.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AD59.

Family & Domainsi

Sequence similaritiesi

Belongs to the ivy family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG146309.
HOGENOMiHOG000117011.
InParanoidiP0AD59.
OMAiDCAAQNI.
OrthoDBiEOG68H883.

Family and domain databases

Gene3Di3.40.1420.10. 1 hit.
InterProiIPR014453. Ihibitor_vertebrate_lysozyme.
[Graphical view]
PIRSFiPIRSF009103. Ivy. 1 hit.
SUPFAMiSSF89872. SSF89872. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AD59-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRISSGGMM FKAITTVAAL VIATSAMAQD DLTISSLAKG ETTKAAFNQM
60 70 80 90 100
VQGHKLPAWV MKGGTYTPAQ TVTLGDETYQ VMSACKPHDC GSQRIAVMWS
110 120 130 140 150
EKSNQMTGLF STIDEKTSQE KLTWLNVNDA LSIDGKTVLF AALTGSLENH

PDGFNFK
Length:157
Mass (Da):16,872
Last modified:December 6, 2005 - v1
Checksum:i9156C5944DFE6D84
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361Missing AA sequence (PubMed:9868784)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08642.1.
U00096 Genomic DNA. Translation: AAC73324.1.
AP009048 Genomic DNA. Translation: BAA77890.1.
PIRiE64746.
RefSeqiNP_414755.1. NC_000913.3.
YP_488517.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73324; AAC73324; b0220.
BAA77890; BAA77890; BAA77890.
GeneIDi12932218.
946530.
KEGGiecj:Y75_p0211.
eco:b0220.
PATRICi32115555. VBIEscCol129921_0222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08642.1.
U00096 Genomic DNA. Translation: AAC73324.1.
AP009048 Genomic DNA. Translation: BAA77890.1.
PIRiE64746.
RefSeqiNP_414755.1. NC_000913.3.
YP_488517.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GPQX-ray1.60A/B29-156[»]
1XS0X-ray1.58A/B/C29-156[»]
ProteinModelPortaliP0AD59.
SMRiP0AD59. Positions 30-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10051N.
STRINGi511145.b0220.

2D gel databases

SWISS-2DPAGEP0AD59.

Proteomic databases

PaxDbiP0AD59.
PRIDEiP0AD59.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73324; AAC73324; b0220.
BAA77890; BAA77890; BAA77890.
GeneIDi12932218.
946530.
KEGGiecj:Y75_p0211.
eco:b0220.
PATRICi32115555. VBIEscCol129921_0222.

Organism-specific databases

EchoBASEiEB3317.
EcoGeneiEG13547. ivy.

Phylogenomic databases

eggNOGiNOG146309.
HOGENOMiHOG000117011.
InParanoidiP0AD59.
OMAiDCAAQNI.
OrthoDBiEOG68H883.

Enzyme and pathway databases

BioCyciEcoCyc:G6104-MONOMER.
ECOL316407:JW0210-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AD59.
PROiP0AD59.

Gene expression databases

GenevestigatoriP0AD59.

Family and domain databases

Gene3Di3.40.1420.10. 1 hit.
InterProiIPR014453. Ihibitor_vertebrate_lysozyme.
[Graphical view]
PIRSFiPIRSF009103. Ivy. 1 hit.
SUPFAMiSSF89872. SSF89872. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: PROTEIN SEQUENCE OF 29-39.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Small genes/gene-products in Escherichia coli K-12."
    Wasinger V.C., Humphery-Smith I.
    FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-38.
    Strain: K12.
  7. "Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme."
    Montchois V., Abergel C., Sturgis J., Jeudy S., Claverie J.-M.
    J. Biol. Chem. 276:18437-18441(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / XL1-Blue.
  8. "Crystallization and preliminary crystallographic study of b0220, an 'ORFan' protein of unknown function from Escherichia coli."
    Abergel C., Monchois V., Chenivesse S., Jeudy S., Claverie J.-M.
    Acta Crystallogr. D 56:1694-1695(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  9. "Structure and evolution of a paradoxical protein family of vertebrate lysozyme inhibitors only found in Gram-negative bacteria."
    Abergel C., Monchois V., Claverie J.-M.
    Submitted (NOV-2001) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiIVY_ECOLI
AccessioniPrimary (citable) accession number: P0AD59
Secondary accession number(s): P45502, P77185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.