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P0AD59 (IVY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inhibitor of vertebrate lysozyme
Gene names
Name:ivy
Synonyms:ykfE
Ordered Locus Names:b0220, JW0210
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Strong inhibitor of lysozyme C.

Subunit structure

Homodimer.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the ivy family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of catalytic activity

Inferred from direct assay Ref.7. Source: EcoCyc

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlysozyme inhibitor activity

Inferred from direct assay Ref.7. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.5 Ref.6
Chain29 – 157129Inhibitor of vertebrate lysozyme
PRO_0000016546

Sites

Site881Important for lysozyme inhibition

Amino acid modifications

Disulfide bond85 ↔ 90

Experimental info

Sequence conflict361Missing AA sequence Ref.6

Secondary structure

............................. 157
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AD59 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 9156C5944DFE6D84

FASTA15716,872
        10         20         30         40         50         60 
MGRISSGGMM FKAITTVAAL VIATSAMAQD DLTISSLAKG ETTKAAFNQM VQGHKLPAWV 

        70         80         90        100        110        120 
MKGGTYTPAQ TVTLGDETYQ VMSACKPHDC GSQRIAVMWS EKSNQMTGLF STIDEKTSQE 

       130        140        150 
KLTWLNVNDA LSIDGKTVLF AALTGSLENH PDGFNFK 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.
Submitted (AUG-1995) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 29-39.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Small genes/gene-products in Escherichia coli K-12."
Wasinger V.C., Humphery-Smith I.
FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-38.
Strain: K12.
[7]"Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme."
Montchois V., Abergel C., Sturgis J., Jeudy S., Claverie J.-M.
J. Biol. Chem. 276:18437-18441(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12 / XL1-Blue.
[8]"Crystallization and preliminary crystallographic study of b0220, an 'ORFan' protein of unknown function from Escherichia coli."
Abergel C., Monchois V., Chenivesse S., Jeudy S., Claverie J.-M.
Acta Crystallogr. D 56:1694-1695(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
[9]"Structure and evolution of a paradoxical protein family of vertebrate lysozyme inhibitors only found in Gram-negative bacteria."
Abergel C., Monchois V., Claverie J.-M.
Submitted (NOV-2001) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U70214 Genomic DNA. Translation: AAB08642.1.
U00096 Genomic DNA. Translation: AAC73324.1.
AP009048 Genomic DNA. Translation: BAA77890.1.
PIRE64746.
RefSeqNP_414755.1. NC_000913.3.
YP_488517.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GPQX-ray1.60A/B29-156[»]
1XS0X-ray1.58A/B/C29-156[»]
ProteinModelPortalP0AD59.
SMRP0AD59. Positions 30-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b0220.

2D gel databases

SWISS-2DPAGEP0AD59.

Proteomic databases

PaxDbP0AD59.
PRIDEP0AD59.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73324; AAC73324; b0220.
BAA77890; BAA77890; BAA77890.
GeneID12932218.
946530.
KEGGecj:Y75_p0211.
eco:b0220.
PATRIC32115555. VBIEscCol129921_0222.

Organism-specific databases

EchoBASEEB3317.
EcoGeneEG13547. ivy.

Phylogenomic databases

eggNOGNOG146309.
HOGENOMHOG000117011.
OMADCAAQNI.
OrthoDBEOG68H883.

Enzyme and pathway databases

BioCycEcoCyc:G6104-MONOMER.
ECOL316407:JW0210-MONOMER.

Gene expression databases

GenevestigatorP0AD59.

Family and domain databases

Gene3D3.40.1420.10. 1 hit.
InterProIPR014453. Ihibitor_vertebrate_lysozyme.
[Graphical view]
PIRSFPIRSF009103. Ivy. 1 hit.
SUPFAMSSF89872. SSF89872. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AD59.
PROP0AD59.

Entry information

Entry nameIVY_ECOLI
AccessionPrimary (citable) accession number: P0AD59
Secondary accession number(s): P45502, P77185
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene