Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P0AD57 (ISPB_ECOLI)

Last modified November 24, 2009. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Octaprenyl-diphosphate synthase
    EC=2.5.1.-
Alternative name(s):
    Octaprenyl pyrophosphate synthetase
      Short name=OPP synthetase
Gene names
Name: ispB
Synonyms: cel, yhbD
Ordered Locus Names: b3187, JW3154
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Hide | Top

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone. Ref.5

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Sequence similarities

Belongs to the FPP/GGPP synthetase family.

Hide | Top

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

transferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Binary interactions

With

Entry

#Exp.

IntAct

Notes

rplBP604221EBI-1131851,EBI-543515

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Octaprenyl-diphosphate synthase
PRO_0000124005

Sites

Metal binding841Magnesium 1 By similarity
Metal binding841Magnesium 2 By similarity
Metal binding881Magnesium 1 By similarity
Metal binding881Magnesium 2 By similarity
Metal binding2111Magnesium 3 By similarity
Binding site451Isopentenyl diphosphate By similarity
Binding site481Isopentenyl diphosphate By similarity
Binding site771Isopentenyl diphosphate By similarity
Binding site931Polyprenyl diphosphate By similarity
Binding site941Isopentenyl diphosphate By similarity
Binding site1701Polyprenyl diphosphate By similarity
Binding site1711Polyprenyl diphosphate By similarity
Binding site2081Polyprenyl diphosphate By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P0AD57-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 08AD7AE7AC230EF8

FASTA32335,217
        10         20         30         40         50         60 
MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM IAVLAARAVG 

        70         80         90        100        110        120 
YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN AAFGNAASVL VGDFIYTRAF 

       130        140        150        160        170        180 
QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL MNVNDPDITE ENYMRVIYSK TARLFEAAAQ 

       190        200        210        220        230        240 
CSGILAGCTP EEEKGLQDYG RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL 

       250        260        270        280        290        300 
LHAMHHGTPE QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ 

       310        320 
VLPDTPWREA LIGLAHIAVQ RDR

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for ribosomal proteins L21 and L27 of Escherichia coli."
Jeong J.H., Kitakawa M.S., Isono S., Isono K.
DNA Seq. 4:59-67(1993) [PubMed: 8312607] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108."
Choi Y.-L., Nishida T., Kawamukai M., Utsumi R., Sakai H., Komano T.
J. Bacteriol. 171:5222-5225(1989) [PubMed: 2670911] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 196-323.
[5]"The identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase."
Asai K., Fujisaki S., Nishimura Y., Nishino T., Okada K., Nakagawa T., Kawamukai M., Matsuda H.
Biochem. Biophys. Res. Commun. 202:340-345(1994) [PubMed: 8037730] [Abstract]
Cited for: FUNCTION.
Strain: K12 / JM109 / ATCC 53323.

Hide | Top

Cross-references

Sequence databases

D13267 Genomic DNA. No translation available.
U18997 Genomic DNA. Translation: AAA57988.1.
U00096 Genomic DNA. Translation: AAC76219.1.
AP009048 Genomic DNA. Translation: BAE77231.1.
X68873 Genomic DNA. Translation: CAA48735.1. Sequence problems.
PIRE65109.
RefSeqAP_003730.1.
NP_417654.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP0AD57. 1 interaction.
STRINGP0AD57.

Genome annotation databases

GeneID947364.
GenomeReviewsGene locus JW3154 in contig AP009048_GR.
Gene locus b3187 in contig U00096_GR.
KEGGecj:JW3154.
eco:b3187.

Organism-specific databases

EchoBASEEB0017.
EcoGeneEG10017. ispB.
CMRSearch...

Phylogenomic databases

HOGENOMP0AD57.
OMAVQQLMNV

Enzyme and pathway databases

BioCycEcoCyc:OPPSYN-MON.
ECOL168927:B3187-MON.
MetaCyc:OPPSYN-MON.

Gene expression databases

GenevestigatorP0AD57.

Family and domain databases

InterProIPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]
Gene3DG3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHERPTHR12001. Polyprenyl_synt. 1 hit.
PfamPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
PROSITEPS00723. POLYPRENYL_SYNTHET_1. 1 hit.
PS00444. POLYPRENYL_SYNTHET_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameISPB_ECOLI
AccessionPrimary (citable) accession number: P0AD57
Secondary accession number(s): P19641, Q2M925
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 24, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents