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Protein

Octaprenyl-diphosphate synthase

Gene

ispB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-octaprenyl diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Isopentenyl diphosphateBy similarity1
Binding sitei48Isopentenyl diphosphateBy similarity1
Binding sitei77Isopentenyl diphosphateBy similarity1
Metal bindingi84Magnesium 1By similarity1
Metal bindingi84Magnesium 2By similarity1
Metal bindingi88Magnesium 1By similarity1
Metal bindingi88Magnesium 2By similarity1
Binding sitei93Polyprenyl diphosphateBy similarity1
Binding sitei94Isopentenyl diphosphateBy similarity1
Binding sitei170Polyprenyl diphosphateBy similarity1
Binding sitei171Polyprenyl diphosphateBy similarity1
Binding sitei208Polyprenyl diphosphateBy similarity1
Metal bindingi211Magnesium 3By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • prenyltransferase activity Source: EcoCyc

GO - Biological processi

  • polyprenol biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:OPPSYN-MONOMER.
ECOL316407:JW3154-MONOMER.
MetaCyc:OPPSYN-MONOMER.
BRENDAi2.5.1.90. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Octaprenyl-diphosphate synthase (EC:2.5.1.90)
Alternative name(s):
All-trans-octaprenyl-diphosphate synthase
Octaprenyl pyrophosphate synthase
Short name:
OPP synthase
Gene namesi
Name:ispB
Synonyms:cel, yhbD
Ordered Locus Names:b3187, JW3154
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10017. ispB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001240051 – 323Octaprenyl-diphosphate synthaseAdd BLAST323

Proteomic databases

PaxDbiP0AD57.
PRIDEiP0AD57.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1131851,EBI-1131851
dlp1O138512EBI-1131851,EBI-7701234From a different organism.
dps1O430912EBI-1131851,EBI-7701164From a different organism.

Protein-protein interaction databases

BioGridi4261111. 123 interactors.
851687. 2 interactors.
IntActiP0AD57. 5 interactors.
MINTiMINT-7384996.
STRINGi511145.b3187.

Structurei

3D structure databases

ProteinModelPortaliP0AD57.
SMRiP0AD57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105EAH. Bacteria.
COG0142. LUCA.
HOGENOMiHOG000009102.
InParanoidiP0AD57.
KOiK02523.
OMAiMRVMEVL.
PhylomeDBiP0AD57.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AD57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM
60 70 80 90 100
IAVLAARAVG YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN
110 120 130 140 150
AAFGNAASVL VGDFIYTRAF QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL
160 170 180 190 200
MNVNDPDITE ENYMRVIYSK TARLFEAAAQ CSGILAGCTP EEEKGLQDYG
210 220 230 240 250
RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL LHAMHHGTPE
260 270 280 290 300
QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ
310 320
VLPDTPWREA LIGLAHIAVQ RDR
Length:323
Mass (Da):35,217
Last modified:December 6, 2005 - v1
Checksum:i08AD7AE7AC230EF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13267 Genomic DNA. No translation available.
U18997 Genomic DNA. Translation: AAA57988.1.
U00096 Genomic DNA. Translation: AAC76219.1.
AP009048 Genomic DNA. Translation: BAE77231.1.
X68873 Genomic DNA. Translation: CAA48735.1. Sequence problems.
PIRiE65109.
RefSeqiNP_417654.1. NC_000913.3.
WP_001047336.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76219; AAC76219; b3187.
BAE77231; BAE77231; BAE77231.
GeneIDi947364.
KEGGiecj:JW3154.
eco:b3187.
PATRICi32121794. VBIEscCol129921_3281.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13267 Genomic DNA. No translation available.
U18997 Genomic DNA. Translation: AAA57988.1.
U00096 Genomic DNA. Translation: AAC76219.1.
AP009048 Genomic DNA. Translation: BAE77231.1.
X68873 Genomic DNA. Translation: CAA48735.1. Sequence problems.
PIRiE65109.
RefSeqiNP_417654.1. NC_000913.3.
WP_001047336.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AD57.
SMRiP0AD57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261111. 123 interactors.
851687. 2 interactors.
IntActiP0AD57. 5 interactors.
MINTiMINT-7384996.
STRINGi511145.b3187.

Proteomic databases

PaxDbiP0AD57.
PRIDEiP0AD57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76219; AAC76219; b3187.
BAE77231; BAE77231; BAE77231.
GeneIDi947364.
KEGGiecj:JW3154.
eco:b3187.
PATRICi32121794. VBIEscCol129921_3281.

Organism-specific databases

EchoBASEiEB0017.
EcoGeneiEG10017. ispB.

Phylogenomic databases

eggNOGiENOG4105EAH. Bacteria.
COG0142. LUCA.
HOGENOMiHOG000009102.
InParanoidiP0AD57.
KOiK02523.
OMAiMRVMEVL.
PhylomeDBiP0AD57.

Enzyme and pathway databases

BioCyciEcoCyc:OPPSYN-MONOMER.
ECOL316407:JW3154-MONOMER.
MetaCyc:OPPSYN-MONOMER.
BRENDAi2.5.1.90. 2026.

Miscellaneous databases

PROiP0AD57.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISPB_ECOLI
AccessioniPrimary (citable) accession number: P0AD57
Secondary accession number(s): P19641, Q2M925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.