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Protein

Octaprenyl diphosphate synthase

Gene

ispB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-octaprenyl diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Isopentenyl diphosphateBy similarity1
Binding sitei48Isopentenyl diphosphateBy similarity1
Binding sitei77Isopentenyl diphosphateBy similarity1
Metal bindingi84Magnesium 1By similarity1
Metal bindingi84Magnesium 2By similarity1
Metal bindingi88Magnesium 1By similarity1
Metal bindingi88Magnesium 2By similarity1
Binding sitei93Polyprenyl diphosphateBy similarity1
Binding sitei94Isopentenyl diphosphateBy similarity1
Binding sitei170Polyprenyl diphosphateBy similarity1
Binding sitei171Polyprenyl diphosphateBy similarity1
Binding sitei208Polyprenyl diphosphateBy similarity1
Metal bindingi211Magnesium 3By similarity1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • prenyltransferase activity Source: EcoCyc

GO - Biological processi

  • polyprenol biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processIsoprene biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:OPPSYN-MONOMER
MetaCyc:OPPSYN-MONOMER
BRENDAi2.5.1.90 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Octaprenyl diphosphate synthase1 Publication (EC:2.5.1.901 Publication)
Alternative name(s):
All-trans-octaprenyl-diphosphate synthase
Octaprenyl pyrophosphate synthase
Short name:
OPP synthase
Gene namesi
Name:ispB
Synonyms:cel, yhbD
Ordered Locus Names:b3187, JW3154
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10017 ispB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001240051 – 323Octaprenyl diphosphate synthaseAdd BLAST323

Proteomic databases

PaxDbiP0AD57
PRIDEiP0AD57

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261111, 137 interactors
851687, 2 interactors
IntActiP0AD57, 5 interactors
MINTiP0AD57
STRINGi316385.ECDH10B_3361

Structurei

3D structure databases

ProteinModelPortaliP0AD57
SMRiP0AD57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105EAH Bacteria
COG0142 LUCA
HOGENOMiHOG000009102
InParanoidiP0AD57
KOiK02523
OMAiGKQMRPM
PhylomeDBiP0AD57

Family and domain databases

Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR000092 Polyprenyl_synt
IPR033749 Polyprenyl_synt_CS
PfamiView protein in Pfam
PF00348 polyprenyl_synt, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
PROSITEiView protein in PROSITE
PS00723 POLYPRENYL_SYNTHASE_1, 1 hit
PS00444 POLYPRENYL_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0AD57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM
60 70 80 90 100
IAVLAARAVG YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN
110 120 130 140 150
AAFGNAASVL VGDFIYTRAF QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL
160 170 180 190 200
MNVNDPDITE ENYMRVIYSK TARLFEAAAQ CSGILAGCTP EEEKGLQDYG
210 220 230 240 250
RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL LHAMHHGTPE
260 270 280 290 300
QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ
310 320
VLPDTPWREA LIGLAHIAVQ RDR
Length:323
Mass (Da):35,217
Last modified:December 6, 2005 - v1
Checksum:i08AD7AE7AC230EF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13267 Genomic DNA No translation available.
U18997 Genomic DNA Translation: AAA57988.1
U00096 Genomic DNA Translation: AAC76219.1
AP009048 Genomic DNA Translation: BAE77231.1
X68873 Genomic DNA Translation: CAA48735.1
PIRiE65109
RefSeqiNP_417654.1, NC_000913.3
WP_001047336.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76219; AAC76219; b3187
BAE77231; BAE77231; BAE77231
GeneIDi947364
KEGGiecj:JW3154
eco:b3187
PATRICifig|1411691.4.peg.3544

Similar proteinsi

Entry informationi

Entry nameiISPB_ECOLI
AccessioniPrimary (citable) accession number: P0AD57
Secondary accession number(s): P19641, Q2M925
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health