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Protein

Sensor histidine kinase BtsS

Gene

btsS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BtsS/BtsR, which is part of a nutrient-sensing regulatory network composed of BtsS/BtsR, the low-affinity pyruvate signaling system YpdA/YpdB and their respective target proteins, YjiY and YhjX. Responds to depletion of nutrients, specifically serine, and the concomitant presence of extracellular pyruvate. BtsS is a high-affinity receptor for extracellular pyruvate that activates BtsR by phosphorylation. Activation of the BtsS/BtsR signaling cascade also suppresses YpdA/YpdB-mediated yhjX induction.4 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.Curated

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: InterPro
  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12007-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase BtsSCurated (EC:2.7.13.3)
Gene namesi
Name:btsS1 Publication
Synonyms:yehU
Ordered Locus Names:b2126, JW5353
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12007. yehU.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3Cytoplasmic1 Publication3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 43Periplasmic1 PublicationAdd BLAST19
Transmembranei44 – 64HelicalSequence analysisAdd BLAST21
Topological domaini65 – 72Cytoplasmic1 Publication8
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Topological domaini94 – 108Periplasmic1 PublicationAdd BLAST15
Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
Topological domaini130 – 140Cytoplasmic1 PublicationAdd BLAST11
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 170Periplasmic1 Publication9
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Topological domaini192 – 561Cytoplasmic1 PublicationAdd BLAST370

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of btsSR has no obvious phenotypic effect under the conditions tested.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi382H → Q: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000138701 – 561Sensor histidine kinase BtsSAdd BLAST561

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AD14.
PRIDEiP0AD14.

Interactioni

Subunit structurei

Interacts with YjiY and YhjX.1 Publication

Protein-protein interaction databases

BioGridi4260444. 239 interactors.
DIPiDIP-11909N.
STRINGi316385.ECDH10B_2281.

Structurei

3D structure databases

ProteinModelPortaliP0AD14.
SMRiP0AD14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 559Histidine kinaseAdd BLAST206

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108HRK. Bacteria.
COG3275. LUCA.
HOGENOMiHOG000242219.
InParanoidiP0AD14.
KOiK02478.
PhylomeDBiP0AD14.

Family and domain databases

Gene3Di3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR010559. Sig_transdc_His_kin_internal.
IPR011620. Sig_transdc_His_kinase_LytS_TM.
PfamiView protein in Pfam
PF07694. 5TM-5TMR_LYT. 1 hit.
PF06580. His_kinase. 1 hit.
SMARTiView protein in SMART
SM00065. GAF. 1 hit.
SUPFAMiSSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AD14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDFNLVLLL LQQMCVFLVI AWLMSKTPLF IPLMQVTVRL PHKFLCYIVF
60 70 80 90 100
SIFCIMGTWF GLHIDDSIAN TRAIGAVMGG LLGGPVVGGL VGLTGGLHRY
110 120 130 140 150
SMGGMTALSC MISTIVEGLL GGLVHSILIR RGRTDKVFNP ITAGAVTFVA
160 170 180 190 200
EMVQMLIILA IARPYEDAVR LVSNIAAPMM VTNTVGAALF MRILLDKRAM
210 220 230 240 250
FEKYTSAFSA TALKVAASTE GILRQGFNEV NSMKVAQVLY QELDIGAVAI
260 270 280 290 300
TDREKLLAFT GIGDDHHLPG KPISSTYTLK AIETGEVVYA DGNEVPYRCS
310 320 330 340 350
LHPQCKLGST LVIPLRGENQ RVMGTIKLYE AKNRLFSSIN RTLGEGIAQL
360 370 380 390 400
LSAQILAGQY ERQKAMLTQS EIKLLHAQVN PHFLFNALNT IKAVIRRDSE
410 420 430 440 450
QASQLVQYLS TFFRKNLKRP SEFVTLADEI EHVNAYLQIE KARFQSRLQV
460 470 480 490 500
NIAIPQELSQ QQLPAFTLQP IVENAIKHGT SQLLDTGRVA ISARREGQHL
510 520 530 540 550
MLEIEDNAGL YQPVTNASGL GMNLVDKRLR ERFGDDYGIS VACEPDSYTR
560
ITLRLPWRDE A
Length:561
Mass (Da):62,092
Last modified:November 22, 2005 - v1
Checksum:i437B09A2010231C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175 – 176IA → MP in AAA60489 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60489.1.
U00096 Genomic DNA. Translation: AAC75187.1.
AP009048 Genomic DNA. Translation: BAE76602.1.
PIRiE64980.
RefSeqiNP_416630.1. NC_000913.3.
WP_001295431.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75187; AAC75187; b2126.
BAE76602; BAE76602; BAE76602.
GeneIDi949027.
KEGGiecj:JW5353.
eco:b2126.
PATRICifig|1411691.4.peg.119.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiBTSS_ECOLI
AccessioniPrimary (citable) accession number: P0AD14
Secondary accession number(s): P33357, P76434, Q2MAV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: July 5, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene