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Protein

Sensor histidine kinase YehU

Gene

yehU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system YehU/YehT, which may be involved in the stationary-phase control network. Activates YehT by phosphorylation.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: InterPro
  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12007-MONOMER.
ECOL316407:JW5353-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase YehU (EC:2.7.13.3)
Gene namesi
Name:yehU
Ordered Locus Names:b2126, JW5353
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12007. yehU.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 4319PeriplasmicSequence analysisAdd
BLAST
Transmembranei44 – 6421HelicalSequence analysisAdd
BLAST
Topological domaini65 – 728CytoplasmicSequence analysis
Transmembranei73 – 9321HelicalSequence analysisAdd
BLAST
Topological domaini94 – 10815PeriplasmicSequence analysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence analysisAdd
BLAST
Topological domaini130 – 14011CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 1709PeriplasmicSequence analysis
Transmembranei171 – 19121HelicalSequence analysisAdd
BLAST
Topological domaini192 – 561370CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of yehUT has no obvious phenotypic effect under the conditions tested.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi382 – 3821H → Q: Lack of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Sensor histidine kinase YehUPRO_0000013870Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AD14.
PRIDEiP0AD14.

Interactioni

Protein-protein interaction databases

BioGridi4260444. 239 interactions.
DIPiDIP-11909N.
STRINGi511145.b2126.

Structurei

3D structure databases

ProteinModelPortaliP0AD14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini354 – 559206Histidine kinaseAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108HRK. Bacteria.
COG3275. LUCA.
HOGENOMiHOG000242219.
InParanoidiP0AD14.
KOiK02478.
OMAiSHFFRSN.
OrthoDBiEOG6RVFWR.
PhylomeDBiP0AD14.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR010559. Sig_transdc_His_kin_internal.
IPR011620. Sig_transdc_His_kinase_LytS_TM.
[Graphical view]
PfamiPF07694. 5TM-5TMR_LYT. 1 hit.
PF13492. GAF_3. 1 hit.
PF06580. His_kinase. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AD14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDFNLVLLL LQQMCVFLVI AWLMSKTPLF IPLMQVTVRL PHKFLCYIVF
60 70 80 90 100
SIFCIMGTWF GLHIDDSIAN TRAIGAVMGG LLGGPVVGGL VGLTGGLHRY
110 120 130 140 150
SMGGMTALSC MISTIVEGLL GGLVHSILIR RGRTDKVFNP ITAGAVTFVA
160 170 180 190 200
EMVQMLIILA IARPYEDAVR LVSNIAAPMM VTNTVGAALF MRILLDKRAM
210 220 230 240 250
FEKYTSAFSA TALKVAASTE GILRQGFNEV NSMKVAQVLY QELDIGAVAI
260 270 280 290 300
TDREKLLAFT GIGDDHHLPG KPISSTYTLK AIETGEVVYA DGNEVPYRCS
310 320 330 340 350
LHPQCKLGST LVIPLRGENQ RVMGTIKLYE AKNRLFSSIN RTLGEGIAQL
360 370 380 390 400
LSAQILAGQY ERQKAMLTQS EIKLLHAQVN PHFLFNALNT IKAVIRRDSE
410 420 430 440 450
QASQLVQYLS TFFRKNLKRP SEFVTLADEI EHVNAYLQIE KARFQSRLQV
460 470 480 490 500
NIAIPQELSQ QQLPAFTLQP IVENAIKHGT SQLLDTGRVA ISARREGQHL
510 520 530 540 550
MLEIEDNAGL YQPVTNASGL GMNLVDKRLR ERFGDDYGIS VACEPDSYTR
560
ITLRLPWRDE A
Length:561
Mass (Da):62,092
Last modified:November 22, 2005 - v1
Checksum:i437B09A2010231C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1762IA → MP in AAA60489 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60489.1.
U00096 Genomic DNA. Translation: AAC75187.1.
AP009048 Genomic DNA. Translation: BAE76602.1.
PIRiE64980.
RefSeqiNP_416630.1. NC_000913.3.
WP_001295431.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75187; AAC75187; b2126.
BAE76602; BAE76602; BAE76602.
GeneIDi949027.
KEGGiecj:JW5353.
eco:b2126.
PATRICi32119589. VBIEscCol129921_2205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60489.1.
U00096 Genomic DNA. Translation: AAC75187.1.
AP009048 Genomic DNA. Translation: BAE76602.1.
PIRiE64980.
RefSeqiNP_416630.1. NC_000913.3.
WP_001295431.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AD14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260444. 239 interactions.
DIPiDIP-11909N.
STRINGi511145.b2126.

Proteomic databases

PaxDbiP0AD14.
PRIDEiP0AD14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75187; AAC75187; b2126.
BAE76602; BAE76602; BAE76602.
GeneIDi949027.
KEGGiecj:JW5353.
eco:b2126.
PATRICi32119589. VBIEscCol129921_2205.

Organism-specific databases

EchoBASEiEB1945.
EcoGeneiEG12007. yehU.

Phylogenomic databases

eggNOGiENOG4108HRK. Bacteria.
COG3275. LUCA.
HOGENOMiHOG000242219.
InParanoidiP0AD14.
KOiK02478.
OMAiSHFFRSN.
OrthoDBiEOG6RVFWR.
PhylomeDBiP0AD14.

Enzyme and pathway databases

BioCyciEcoCyc:EG12007-MONOMER.
ECOL316407:JW5353-MONOMER.

Miscellaneous databases

PROiP0AD14.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR010559. Sig_transdc_His_kin_internal.
IPR011620. Sig_transdc_His_kinase_LytS_TM.
[Graphical view]
PfamiPF07694. 5TM-5TMR_LYT. 1 hit.
PF13492. GAF_3. 1 hit.
PF06580. His_kinase. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / BHB2600.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOPHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "First insights into the unexplored two-component system YehU/YehT in Escherichia coli."
    Kraxenberger T., Fried L., Behr S., Jung K.
    J. Bacteriol. 194:4272-4284(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF HIS-382.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiYEHU_ECOLI
AccessioniPrimary (citable) accession number: P0AD14
Secondary accession number(s): P33357, P76434, Q2MAV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.