Transcriptional regulatory protein CusR - Escherichia coli (strain K12)

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P0ACZ8 (CUSR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein CusR

Gene names

Name:	cusR
Synonyms:	ylcA
Ordered Locus Names:	b0571, JW0560

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA and plasmid pRJ1004 gene pcoE in response to increasing levels of copper ions, and probably silver ions. Can also increase the basal-level expression of copper resistance gene operon pcoABCD. Ref.1 Ref.7 Ref.8
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by CusS. Ref.9
Miscellaneous	The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	Copper DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC phosphorelay signal transduction system Inferred from direct assay Ref.9. Source: EcoCyc positive regulation of gene expression Inferred from mutant phenotype Ref.1. Source: EcoCyc regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.1. Source: EcoCyc response to copper ion Inferred from direct assay Ref.1. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell protein-DNA complex Inferred from direct assay PubMed 15773991. Source: EcoCyc protein-DNA-RNA complex Inferred from direct assay PubMed 15773991. Source: EcoCyc
Molecular_function	core promoter sequence-specific DNA binding Inferred from direct assay PubMed 15773991. Source: EcoCyc phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 227

227

Transcriptional regulatory protein CusR

PRO_0000081063

Regions

Domain

1 – 116

116

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P0ACZ8 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 5CE129597FAE43C0
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FASTA

227

25,395

        10         20         30         40         50         60 
MKLLIVEDEK KTGEYLTKGL TEAGFVVDLA DNGLNGYHLA MTGDYDLIIL DIMLPDVNGW 

        70         80         90        100        110        120 
DIVRMLRSAN KGMPILLLTA LGTIEHRVKG LELGADDYLV KPFAFAELLA RVRTLLRRGA 

       130        140        150        160        170        180 
AVIIESQFQV ADLMVDLVSR KVTRSGTRIT LTSKEFTLLE FFLRHQGEVL PRSLIASQVW 

       190        200        210        220 
DMNFDSDTNA IDVAVKRLRG KIDNDFEPKL IQTVRGVGYM LEVPDGQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12." Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V. J. Bacteriol. 182:5864-5871(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: K12 / DH5-alpha.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	Morel-Deville F., Ehrlich S.D., Morel P. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-94. Strain: HVC45.
[7]	"The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli." Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V. J. Biol. Chem. 276:30670-30677(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN COPPER HOMEOSTASIS. Strain: K12.
[8]	"The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions." Franke S., Grass G., Nies D.H. Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROBABLE FUNCTION IN SILVER HOMEOSTASIS. Strain: K38.
[9]	"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A. J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF245661 Genomic DNA. Translation: AAF70175.1. U82598 Genomic DNA. Translation: AAB40769.1. U00096 Genomic DNA. Translation: AAC73672.1. AP009048 Genomic DNA. Translation: BAA35205.1. U82579 Genomic DNA. Translation: AAB41754.1.
PIR	A64790.
RefSeq	NP_415103.1. NC_000913.2. YP_488858.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACZ8.
SMR	P0ACZ8. Positions 1-224.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0ACZ8. 7 interactions.
STRING	511145.b0571.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73672; AAC73672; b0571. BAA35205; BAA35205; BAA35205.
GeneID	12930897. 945003.
KEGG	ecj:Y75_p0558. eco:b0571.
PATRIC	32116310. VBIEscCol129921_0595.
Organism-specific databases
EchoBASE	EB3612.
EcoGene	EG13851. cusR.
Phylogenomic databases
eggNOG	COG0745.
HOGENOM	HOG000034815.
KO	K07665.
OMA	AMTEPYD.
ProtClustDB	PRK09836.
Enzyme and pathway databases
BioCyc	EcoCyc:G6319-MONOMER. ECOL316407:JW0560-MONOMER.
Gene expression databases
Genevestigator	P0ACZ8.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR001867. Sig_transdc_resp-reg_C. IPR006291. Sig_transdc_resp-reg_metal. IPR001789. Sig_transdc_resp-reg_receiver. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00072. Response_reg. 1 hit. PF00486. Trans_reg_C. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. SM00862. Trans_reg_C. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
TIGRFAMs	TIGR01387. cztR_silR_copR. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CUSR_ECOLI

Accession

Primary (citable) accession number: P0ACZ8
Secondary accession number(s): P77380

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents