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P0ACZ8

- CUSR_ECOLI

UniProt

P0ACZ8 - CUSR_ECOLI

Protein

Transcriptional regulatory protein CusR

Gene

cusR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA and plasmid pRJ1004 gene pcoE in response to increasing levels of copper ions, and probably silver ions. Can also increase the basal-level expression of copper resistance gene operon pcoABCD.2 Publications

    GO - Molecular functioni

    1. core promoter sequence-specific DNA binding Source: EcoCyc
    2. DNA binding Source: EcoCyc
    3. identical protein binding Source: IntAct
    4. phosphorelay response regulator activity Source: InterPro

    GO - Biological processi

    1. phosphorelay signal transduction system Source: EcoCyc
    2. positive regulation of gene expression Source: EcoCyc
    3. regulation of transcription, DNA-templated Source: EcoCyc
    4. response to copper ion Source: EcoCyc
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    Copper, DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G6319-MONOMER.
    ECOL316407:JW0560-MONOMER.
    RETL1328306-WGS:GSTH-1298-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein CusR
    Gene namesi
    Name:cusR
    Synonyms:ylcA
    Ordered Locus Names:b0571, JW0560
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13851. cusR.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. protein-DNA complex Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Transcriptional regulatory protein CusRPRO_0000081063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei51 – 5114-aspartylphosphatePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by CusS.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP0ACZ8.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1118807,EBI-1118807

    Protein-protein interaction databases

    DIPiDIP-9348N.
    IntActiP0ACZ8. 9 interactions.
    STRINGi511145.b0571.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACZ8.
    SMRiP0ACZ8. Positions 1-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 116116Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034815.
    KOiK07665.
    OMAiIDVCKAI.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP0ACZ8.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR006291. Sig_transdc_resp-reg_metal.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    TIGRFAMsiTIGR01387. cztR_silR_copR. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACZ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLIVEDEK KTGEYLTKGL TEAGFVVDLA DNGLNGYHLA MTGDYDLIIL    50
    DIMLPDVNGW DIVRMLRSAN KGMPILLLTA LGTIEHRVKG LELGADDYLV 100
    KPFAFAELLA RVRTLLRRGA AVIIESQFQV ADLMVDLVSR KVTRSGTRIT 150
    LTSKEFTLLE FFLRHQGEVL PRSLIASQVW DMNFDSDTNA IDVAVKRLRG 200
    KIDNDFEPKL IQTVRGVGYM LEVPDGQ 227
    Length:227
    Mass (Da):25,395
    Last modified:November 22, 2005 - v1
    Checksum:i5CE129597FAE43C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF245661 Genomic DNA. Translation: AAF70175.1.
    U82598 Genomic DNA. Translation: AAB40769.1.
    U00096 Genomic DNA. Translation: AAC73672.1.
    AP009048 Genomic DNA. Translation: BAA35205.1.
    U82579 Genomic DNA. Translation: AAB41754.1.
    PIRiA64790.
    RefSeqiNP_415103.1. NC_000913.3.
    YP_488858.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73672; AAC73672; b0571.
    BAA35205; BAA35205; BAA35205.
    GeneIDi12930897.
    945003.
    KEGGiecj:Y75_p0558.
    eco:b0571.
    PATRICi32116310. VBIEscCol129921_0595.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF245661 Genomic DNA. Translation: AAF70175.1 .
    U82598 Genomic DNA. Translation: AAB40769.1 .
    U00096 Genomic DNA. Translation: AAC73672.1 .
    AP009048 Genomic DNA. Translation: BAA35205.1 .
    U82579 Genomic DNA. Translation: AAB41754.1 .
    PIRi A64790.
    RefSeqi NP_415103.1. NC_000913.3.
    YP_488858.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0ACZ8.
    SMRi P0ACZ8. Positions 1-224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9348N.
    IntActi P0ACZ8. 9 interactions.
    STRINGi 511145.b0571.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73672 ; AAC73672 ; b0571 .
    BAA35205 ; BAA35205 ; BAA35205 .
    GeneIDi 12930897.
    945003.
    KEGGi ecj:Y75_p0558.
    eco:b0571.
    PATRICi 32116310. VBIEscCol129921_0595.

    Organism-specific databases

    EchoBASEi EB3612.
    EcoGenei EG13851. cusR.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034815.
    KOi K07665.
    OMAi IDVCKAI.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P0ACZ8.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6319-MONOMER.
    ECOL316407:JW0560-MONOMER.
    RETL1328306-WGS:GSTH-1298-MONOMER.

    Miscellaneous databases

    PROi P0ACZ8.

    Gene expression databases

    Genevestigatori P0ACZ8.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR006291. Sig_transdc_resp-reg_metal.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    TIGRFAMsi TIGR01387. cztR_silR_copR. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12."
      Munson G.P., Lam D.L., Outten F.W., O'Halloran T.V.
      J. Bacteriol. 182:5864-5871(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: K12 / DH5-alpha.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. Morel-Deville F., Ehrlich S.D., Morel P.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-94.
      Strain: HVC45.
    7. "The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli."
      Outten F.W., Huffman D.L., Hale J.A., O'Halloran T.V.
      J. Biol. Chem. 276:30670-30677(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN COPPER HOMEOSTASIS.
      Strain: K12.
    8. "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions."
      Franke S., Grass G., Nies D.H.
      Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROBABLE FUNCTION IN SILVER HOMEOSTASIS.
      Strain: K38.
    9. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
      Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
      J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiCUSR_ECOLI
    AccessioniPrimary (citable) accession number: P0ACZ8
    Secondary accession number(s): P77380
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3