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P0ACZ4

- EVGA_ECOLI

UniProt

P0ACZ4 - EVGA_ECOLI

Protein

Positive transcription regulator EvgA

Gene

evgA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system EvgS/EvgA. Regulates the expression of emrKY operon and yfdX. Seems also to control expression of at least one other multidrug efflux operon.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi161 – 18020H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. phosphorelay response regulator activity Source: InterPro
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding Source: EcoCyc
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: EcoCyc
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EVGA-MONOMER.
    ECOL316407:JW2366-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Positive transcription regulator EvgA
    Gene namesi
    Name:evgA
    Ordered Locus Names:b2369, JW2366
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11609. evgA.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Positive transcription regulator EvgAPRO_0000081035Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 5214-aspartylphosphatePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by EvgS.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP0ACZ4.
    PRIDEiP0ACZ4.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ACZ4.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    glnGP0AFB83EBI-548694,EBI-1113197
    proQP455773EBI-548694,EBI-8767901

    Protein-protein interaction databases

    DIPiDIP-48087N.
    IntActiP0ACZ4. 15 interactions.
    MINTiMINT-1282623.
    STRINGi511145.b2369.

    Structurei

    Secondary structure

    1
    204
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi10 – 2213
    Beta strandi25 – 3410
    Helixi37 – 448
    Beta strandi47 – 526
    Beta strandi56 – 583
    Helixi60 – 6910
    Beta strandi74 – 807
    Helixi88 – 936
    Beta strandi97 – 1015
    Helixi102 – 1043
    Helixi107 – 11711
    Helixi127 – 1293

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3F6CX-ray1.45A/B2-133[»]
    ProteinModelPortaliP0ACZ4.
    SMRiP0ACZ4. Positions 1-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ACZ4.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 117117Response regulatoryPROSITE-ProRule annotationAdd
    BLAST
    Domaini137 – 20266HTH luxR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2197.
    HOGENOMiHOG000034813.
    KOiK07690.
    OMAiMTIMSAT.
    OrthoDBiEOG69GZGV.
    PhylomeDBiP0ACZ4.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR000792. Tscrpt_reg_LuxR_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00196. GerE. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PRINTSiPR00038. HTHLUXR.
    SMARTiSM00421. HTH_LUXR. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEiPS00622. HTH_LUXR_1. 1 hit.
    PS50043. HTH_LUXR_2. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACZ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAIIIDDHP LAIAAIRNLL IKNDIEILAE LTEGGSAVQR VETLKPDIVI    50
    IDVDIPGVNG IQVLETLRKR QYSGIIIIVS AKNDHFYGKH CADAGANGFV 100
    SKKEGMNNII AAIEAAKNGY CYFPFSLNRF VGSLTSDQQK LDSLSKQEIS 150
    VMRYILDGKD NNDIAEKMFI SNKTVSTYKS RLMEKLECKS LMDLYTFAQR 200
    NKIG 204
    Length:204
    Mass (Da):22,690
    Last modified:November 22, 2005 - v1
    Checksum:iE68F932F3729FBBB
    GO

    Mass spectrometryi

    Molecular mass is 22693 Da from positions 1 - 204. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14008 Genomic DNA. Translation: BAA03107.1.
    D11142 Genomic DNA. Translation: BAA01918.1.
    U00096 Genomic DNA. Translation: AAC75428.1.
    AP009048 Genomic DNA. Translation: BAA16240.1.
    PIRiJU0220.
    RefSeqiNP_416870.1. NC_000913.3.
    YP_490611.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75428; AAC75428; b2369.
    BAA16240; BAA16240; BAA16240.
    GeneIDi12931564.
    946841.
    KEGGiecj:Y75_p2336.
    eco:b2369.
    PATRICi32120117. VBIEscCol129921_2467.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14008 Genomic DNA. Translation: BAA03107.1 .
    D11142 Genomic DNA. Translation: BAA01918.1 .
    U00096 Genomic DNA. Translation: AAC75428.1 .
    AP009048 Genomic DNA. Translation: BAA16240.1 .
    PIRi JU0220.
    RefSeqi NP_416870.1. NC_000913.3.
    YP_490611.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3F6C X-ray 1.45 A/B 2-133 [» ]
    ProteinModelPortali P0ACZ4.
    SMRi P0ACZ4. Positions 1-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48087N.
    IntActi P0ACZ4. 15 interactions.
    MINTi MINT-1282623.
    STRINGi 511145.b2369.

    Proteomic databases

    PaxDbi P0ACZ4.
    PRIDEi P0ACZ4.

    Protocols and materials databases

    DNASUi 946841.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75428 ; AAC75428 ; b2369 .
    BAA16240 ; BAA16240 ; BAA16240 .
    GeneIDi 12931564.
    946841.
    KEGGi ecj:Y75_p2336.
    eco:b2369.
    PATRICi 32120117. VBIEscCol129921_2467.

    Organism-specific databases

    EchoBASEi EB1566.
    EcoGenei EG11609. evgA.

    Phylogenomic databases

    eggNOGi COG2197.
    HOGENOMi HOG000034813.
    KOi K07690.
    OMAi MTIMSAT.
    OrthoDBi EOG69GZGV.
    PhylomeDBi P0ACZ4.

    Enzyme and pathway databases

    BioCyci EcoCyc:EVGA-MONOMER.
    ECOL316407:JW2366-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0ACZ4.
    PROi P0ACZ4.

    Gene expression databases

    Genevestigatori P0ACZ4.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR000792. Tscrpt_reg_LuxR_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00196. GerE. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PRINTSi PR00038. HTHLUXR.
    SMARTi SM00421. HTH_LUXR. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEi PS00622. HTH_LUXR_1. 1 hit.
    PS50043. HTH_LUXR_2. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Newly identified genes involved in the signal transduction of Escherichia coli K-12."
      Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M.
      Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12."
      Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M.
      Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins."
      Perraud A.-L., Kimmel B., Weiss V., Gross R.
      Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Dimerization of signalling modules of the EvgAS and BvgAS phosphorelay systems."
      Perraud A.-L., Rippe K., Bantscheff M., Glocker M., Lucassen M., Jung K., Sebald W., Weiss V., Gross R.
      Biochim. Biophys. Acta 1478:341-354(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MASS SPECTROMETRY.
    8. "Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12."
      Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R.
      Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12.
    9. "Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters."
      Nishino K., Yamaguchi A.
      J. Bacteriol. 183:1455-1458(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / W3104 / ATCC 19020.

    Entry informationi

    Entry nameiEVGA_ECOLI
    AccessioniPrimary (citable) accession number: P0ACZ4
    Secondary accession number(s): P30854
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3