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P0ACZ4

- EVGA_ECOLI

UniProt

P0ACZ4 - EVGA_ECOLI

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Protein

Positive transcription regulator EvgA

Gene

evgA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system EvgS/EvgA. Regulates the expression of emrKY operon and yfdX. Seems also to control expression of at least one other multidrug efflux operon.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi161 – 18020H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: EcoCyc
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. phosphorelay signal transduction system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: EcoCyc
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EVGA-MONOMER.
ECOL316407:JW2366-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Positive transcription regulator EvgA
Gene namesi
Name:evgA
Ordered Locus Names:b2369, JW2366
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11609. evgA.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Positive transcription regulator EvgAPRO_0000081035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 5214-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by EvgS.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0ACZ4.
PRIDEiP0ACZ4.

Expressioni

Gene expression databases

GenevestigatoriP0ACZ4.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
glnGP0AFB83EBI-548694,EBI-1113197
proQP455773EBI-548694,EBI-8767901

Protein-protein interaction databases

DIPiDIP-48087N.
IntActiP0ACZ4. 15 interactions.
MINTiMINT-1282623.
STRINGi511145.b2369.

Structurei

Secondary structure

1
204
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65
Helixi10 – 2213
Beta strandi25 – 3410
Helixi37 – 448
Beta strandi47 – 526
Beta strandi56 – 583
Helixi60 – 6910
Beta strandi74 – 807
Helixi88 – 936
Beta strandi97 – 1015
Helixi102 – 1043
Helixi107 – 11711
Helixi127 – 1293

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F6CX-ray1.45A/B2-133[»]
ProteinModelPortaliP0ACZ4.
SMRiP0ACZ4. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACZ4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 117117Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini137 – 20266HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2197.
HOGENOMiHOG000034813.
InParanoidiP0ACZ4.
KOiK07690.
OMAiMTIMSAT.
OrthoDBiEOG69GZGV.
PhylomeDBiP0ACZ4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACZ4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNAIIIDDHP LAIAAIRNLL IKNDIEILAE LTEGGSAVQR VETLKPDIVI
60 70 80 90 100
IDVDIPGVNG IQVLETLRKR QYSGIIIIVS AKNDHFYGKH CADAGANGFV
110 120 130 140 150
SKKEGMNNII AAIEAAKNGY CYFPFSLNRF VGSLTSDQQK LDSLSKQEIS
160 170 180 190 200
VMRYILDGKD NNDIAEKMFI SNKTVSTYKS RLMEKLECKS LMDLYTFAQR

NKIG
Length:204
Mass (Da):22,690
Last modified:November 22, 2005 - v1
Checksum:iE68F932F3729FBBB
GO

Mass spectrometryi

Molecular mass is 22693 Da from positions 1 - 204. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14008 Genomic DNA. Translation: BAA03107.1.
D11142 Genomic DNA. Translation: BAA01918.1.
U00096 Genomic DNA. Translation: AAC75428.1.
AP009048 Genomic DNA. Translation: BAA16240.1.
PIRiJU0220.
RefSeqiNP_416870.1. NC_000913.3.
YP_490611.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75428; AAC75428; b2369.
BAA16240; BAA16240; BAA16240.
GeneIDi12931564.
946841.
KEGGiecj:Y75_p2336.
eco:b2369.
PATRICi32120117. VBIEscCol129921_2467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14008 Genomic DNA. Translation: BAA03107.1 .
D11142 Genomic DNA. Translation: BAA01918.1 .
U00096 Genomic DNA. Translation: AAC75428.1 .
AP009048 Genomic DNA. Translation: BAA16240.1 .
PIRi JU0220.
RefSeqi NP_416870.1. NC_000913.3.
YP_490611.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3F6C X-ray 1.45 A/B 2-133 [» ]
ProteinModelPortali P0ACZ4.
SMRi P0ACZ4. Positions 1-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48087N.
IntActi P0ACZ4. 15 interactions.
MINTi MINT-1282623.
STRINGi 511145.b2369.

Proteomic databases

PaxDbi P0ACZ4.
PRIDEi P0ACZ4.

Protocols and materials databases

DNASUi 946841.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75428 ; AAC75428 ; b2369 .
BAA16240 ; BAA16240 ; BAA16240 .
GeneIDi 12931564.
946841.
KEGGi ecj:Y75_p2336.
eco:b2369.
PATRICi 32120117. VBIEscCol129921_2467.

Organism-specific databases

EchoBASEi EB1566.
EcoGenei EG11609. evgA.

Phylogenomic databases

eggNOGi COG2197.
HOGENOMi HOG000034813.
InParanoidi P0ACZ4.
KOi K07690.
OMAi MTIMSAT.
OrthoDBi EOG69GZGV.
PhylomeDBi P0ACZ4.

Enzyme and pathway databases

BioCyci EcoCyc:EVGA-MONOMER.
ECOL316407:JW2366-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ACZ4.
PROi P0ACZ4.

Gene expression databases

Genevestigatori P0ACZ4.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PRINTSi PR00038. HTHLUXR.
SMARTi SM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view ]
SUPFAMi SSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEi PS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Newly identified genes involved in the signal transduction of Escherichia coli K-12."
    Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M.
    Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12."
    Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M.
    Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins."
    Perraud A.-L., Kimmel B., Weiss V., Gross R.
    Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Dimerization of signalling modules of the EvgAS and BvgAS phosphorelay systems."
    Perraud A.-L., Rippe K., Bantscheff M., Glocker M., Lucassen M., Jung K., Sebald W., Weiss V., Gross R.
    Biochim. Biophys. Acta 1478:341-354(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MASS SPECTROMETRY.
  8. "Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12."
    Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R.
    Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12.
  9. "Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters."
    Nishino K., Yamaguchi A.
    J. Bacteriol. 183:1455-1458(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / W3104 / ATCC 19020.

Entry informationi

Entry nameiEVGA_ECOLI
AccessioniPrimary (citable) accession number: P0ACZ4
Secondary accession number(s): P30854
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3