P0ACZ4 (EVGA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Positive transcription regulator EvgA

Gene names

Name:	evgA
Ordered Locus Names:	b2369, JW2366

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system EvgS/EvgA. Regulates the expression of emrKY operon and yfdX. Seems also to control expression of at least one other multidrug efflux operon.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by EvgS.
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain. Contains 1 response regulatory domain.
Mass spectrometry	Molecular mass is 22693 Da from positions 1 - 204. Determined by MALDI. Ref.7

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 19352034. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from direct assay PubMed 15489450. Source: EcoCyc sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

Positive transcription regulator EvgA

PRO_0000081035

Regions

Domain

1 – 117

117

Response regulatory

Domain

137 – 202

66

HTH luxR-type

DNA binding

161 – 180

20

H-T-H motif By similarity

Amino acid modifications

Modified residue

52

1

4-aspartylphosphate By similarity

Secondary structure

1

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204

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

10 – 22

13

Beta strand

25 – 34

10

Helix

37 – 44

8

Beta strand

47 – 52

6

Beta strand

56 – 58

3

Helix

60 – 69

10

Beta strand

74 – 80

7

Helix

88 – 93

6

Beta strand

97 – 101

5

Helix

102 – 104

3

Helix

107 – 117

11

Helix

127 – 129

3

Sequences

	Sequence		Length	Mass (Da)
	P0ACZ4 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E68F932F3729FBBB	FASTA	204	22,690
	10 20 30 40 50 60 MNAIIIDDHP LAIAAIRNLL IKNDIEILAE LTEGGSAVQR VETLKPDIVI IDVDIPGVNG 70 80 90 100 110 120 IQVLETLRKR QYSGIIIIVS AKNDHFYGKH CADAGANGFV SKKEGMNNII AAIEAAKNGY 130 140 150 160 170 180 CYFPFSLNRF VGSLTSDQQK LDSLSKQEIS VMRYILDGKD NNDIAEKMFI SNKTVSTYKS 190 200 RLMEKLECKS LMDLYTFAQR NKIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Newly identified genes involved in the signal transduction of Escherichia coli K-12." Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M. Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12." Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M. Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins." Perraud A.-L., Kimmel B., Weiss V., Gross R. Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Dimerization of signalling modules of the EvgAS and BvgAS phosphorelay systems." Perraud A.-L., Rippe K., Bantscheff M., Glocker M., Lucassen M., Jung K., Sebald W., Weiss V., Gross R. Biochim. Biophys. Acta 1478:341-354(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MASS SPECTROMETRY.
[8]	"Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12." Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R. Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.
[9]	"Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters." Nishino K., Yamaguchi A. J. Bacteriol. 183:1455-1458(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / W3104 / ATCC 19020.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D14008 Genomic DNA. Translation: BAA03107.1.
D11142 Genomic DNA. Translation: BAA01918.1.
U00096 Genomic DNA. Translation: AAC75428.1.
AP009048 Genomic DNA. Translation: BAA16240.1.

PIR

JU0220.

RefSeq

NP_416870.1. NC_000913.2.
YP_490611.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3F6C	X-ray	1.45	A/B	2-133	[»]

ProteinModelPortal

P0ACZ4.

SMR

P0ACZ4. Positions 1-178.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48087N.

IntAct

P0ACZ4. 5 interactions.

MINT

MINT-1282623.

STRING

511145.b2369.

Proteomic databases

PaxDb

P0ACZ4.

PRIDE

P0ACZ4.

Protocols and materials databases

DNASU

946841.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75428; AAC75428; b2369.
BAA16240; BAA16240; BAA16240.

GeneID

12931564.
946841.

KEGG

ecj:Y75_p2336.
eco:b2369.

PATRIC

32120117. VBIEscCol129921_2467.

Organism-specific databases

EchoBASE

EB1566.

EcoGene

EG11609. evgA.

Phylogenomic databases

eggNOG

COG2197.

HOGENOM

HOG000034813.

KO

K07690.

OMA

NDIADKM.

ProtClustDB

PRK09958.

Enzyme and pathway databases

BioCyc

EcoCyc:EVGA-MONOMER.
ECOL316407:JW2366-MONOMER.

Gene expression databases

Genevestigator

P0ACZ4.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]

PRINTS

PR00038. HTHLUXR.

SMART

SM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]

SUPFAM

SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
SSF52172. CheY_like. 1 hit.

PROSITE

PS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0ACZ4.

Entry information

Entry name

EVGA_ECOLI

Accession

Primary (citable) accession number: P0ACZ4
Secondary accession number(s): P30854

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program